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TitleConformational control of Cas9 by CRISPR hybrid RNA-DNA guides mitigates off-target activity in T cells.
Journal, issue, pagesMol. Cell, Vol. 81, Page 3637-3649.e5, Year 2021
Publish dateJun 22, 2021 (structure data deposition date)
AuthorsDonohoue, P.D. / Pacesa, M. / Lau, E. / Vidal, B. / Irby, M.J. / Nyer, D.B. / Rotstein, T. / Banh, L. / Toh, M.S. / Gibson, J. ...Donohoue, P.D. / Pacesa, M. / Lau, E. / Vidal, B. / Irby, M.J. / Nyer, D.B. / Rotstein, T. / Banh, L. / Toh, M.S. / Gibson, J. / Kohrs, B. / Baek, K. / Owen, A.L.G. / Slorach, E.M. / van Overbeek, M. / Fuller, C.K. / May, A.P. / Jinek, M. / Cameron, P.
External linksMol. Cell / PubMed:34478654
MethodsX-ray diffraction
Resolution2.15 - 2.75 Å
Structure data

PDB-7ox7:
Target-bound SpCas9 complex with TRAC chimeric RNA-DNA guide
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-7ox8:
Target-bound SpCas9 complex with TRAC full RNA guide
Method: X-RAY DIFFRACTION / Resolution: 2.75 Å

PDB-7ox9:
Target-bound SpCas9 complex with AAVS1 all-RNA guide
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-7oxa:
Target-bound SpCas9 complex with AAVS1 chimeric RNA-DNA guide
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-HOH:
WATER

Source
  • streptococcus pyogenes serotype m1 (bacteria)
  • synthetic construct (others)
KeywordsHYDROLASE / Cas9 / CRISPR / chRDNA / TRAC / nuclease / sgRNA / AAVS1

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