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- PDB-7lrm: Structure of HIV-1 Reverse Transcriptase in complex with DNA, dCT... -

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Basic information

Entry
Database: PDB / ID: 7lrm
TitleStructure of HIV-1 Reverse Transcriptase in complex with DNA, dCTP, and CA(2+) ion
Components
  • (Reverse transcriptase ...) x 2
  • DNA/RNA (38-MER)
KeywordsTRANSFERASE/DNA / Human immunodeficiency virus 1 / Protein/dsDNA / Aptamer / dNTP / TRANSFERASE / TRANSFERASE-DNA complex
Function / homology
Function and homology information


RNA-directed DNA polymerase activity / HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase ...RNA-directed DNA polymerase activity / HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / telomerase activity / viral penetration into host nucleus / RNA stem-loop binding / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / host cell / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / aspartic-type endopeptidase activity / Hydrolases; Acting on ester bonds / DNA-directed DNA polymerase activity / symbiont-mediated suppression of host gene expression / symbiont entry into host cell / viral translational frameshifting / lipid binding / host cell nucleus / host cell plasma membrane / virion membrane / structural molecule activity / proteolysis / DNA binding / zinc ion binding / membrane
Similarity search - Function
Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain ...Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain / Integrase, C-terminal, retroviral / Integrase DNA binding domain profile. / Immunodeficiency lentiviral matrix, N-terminal / gag gene protein p17 (matrix protein) / RNase H / Integrase core domain / Integrase, catalytic core / Integrase catalytic domain profile. / Retropepsin-like catalytic domain / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Retroviral nucleocapsid Gag protein p24, C-terminal domain / Gag protein p24 C-terminal domain / RNase H type-1 domain profile. / Ribonuclease H domain / Retropepsins / Retroviral aspartyl protease / Aspartyl protease, retroviral-type family profile. / Peptidase A2A, retrovirus, catalytic / Reverse transcriptase domain / Reverse transcriptase (RNA-dependent DNA polymerase) / Reverse transcriptase (RT) catalytic domain profile. / Retrovirus capsid, C-terminal / Retroviral matrix protein / Retrovirus capsid, N-terminal / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Aspartic peptidase, active site / Eukaryotic and viral aspartyl proteases active site. / Aspartic peptidase domain superfamily / Ribonuclease H superfamily / Ribonuclease H-like superfamily / Reverse transcriptase/Diguanylate cyclase domain / DNA/RNA polymerase superfamily
Similarity search - Domain/homology
2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE / DNA / DNA (> 10) / Gag-Pol polyprotein
Similarity search - Component
Biological speciesHuman immunodeficiency virus type 1
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.14 Å
AuthorsHoang, A. / Ruiz, F.X. / Arnold, E.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R37 AI027690 United States
CitationJournal: Drug Discov Today / Year: 2022
Title: Structural basis of HIV inhibition by L-nucleosides: Opportunities for drug development and repurposing.
Authors: Ruiz, F.X. / Hoang, A. / Dilmore, C.R. / DeStefano, J.J. / Arnold, E.
History
DepositionFeb 16, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 23, 2022Provider: repository / Type: Initial release
Revision 1.1Mar 9, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.name
Revision 1.2Jul 6, 2022Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title
Revision 1.3Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Reverse transcriptase p66
B: Reverse transcriptase p51
C: Reverse transcriptase p66
D: Reverse transcriptase p51
F: DNA/RNA (38-MER)
E: DNA/RNA (38-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)254,46726
Polymers251,4706
Non-polymers2,99620
Water2,702150
1
A: Reverse transcriptase p66
B: Reverse transcriptase p51
E: DNA/RNA (38-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)127,23313
Polymers125,7353
Non-polymers1,49810
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area15080 Å2
ΔGint-51 kcal/mol
Surface area46410 Å2
MethodPISA
2
C: Reverse transcriptase p66
D: Reverse transcriptase p51
F: DNA/RNA (38-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)127,23313
Polymers125,7353
Non-polymers1,49810
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area15120 Å2
ΔGint-60 kcal/mol
Surface area46520 Å2
MethodPISA
Unit cell
Length a, b, c (Å)89.663, 131.247, 137.491
Angle α, β, γ (deg.)90.000, 97.717, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Reverse transcriptase ... , 2 types, 4 molecules ACBD

#1: Protein Reverse transcriptase p66


Mass: 63874.133 Da / Num. of mol.: 2 / Mutation: C280S, D498N
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 / Production host: Escherichia coli (E. coli)
References: UniProt: P03366, RNA-directed DNA polymerase, DNA-directed DNA polymerase, retroviral ribonuclease H
#2: Protein Reverse transcriptase p51


Mass: 50096.539 Da / Num. of mol.: 2 / Mutation: C280S
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 / Production host: Escherichia coli (E. coli) / References: UniProt: P03366, RNA-directed DNA polymerase

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DNA chain / Sugars , 2 types, 4 molecules FE

#3: DNA chain DNA/RNA (38-MER)


Mass: 11764.525 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#4: Polysaccharide beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose


Type: oligosaccharide / Mass: 342.297 Da / Num. of mol.: 2 / Source method: obtained synthetically
DescriptorTypeProgram
DFrufb2-1DGlcpaGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,2,1/[ha122h-2b_2-5][a2122h-1a_1-5]/1-2/a2-b1WURCSPDB2Glycan 1.1.0
[][b-D-Fruf]{[(2+1)][a-D-Glcp]{}}LINUCSPDB-CARE

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Non-polymers , 5 types, 168 molecules

#5: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#6: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: C3H8O3
#7: Chemical ChemComp-DCP / 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE


Mass: 467.157 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C9H16N3O13P3 / Feature type: SUBJECT OF INVESTIGATION
#8: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca
#9: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 150 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.08 Å3/Da / Density % sol: 60.11 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.2
Details: 10-12% PEG 8000, 50 MM BISTRIS-PROPANE PH 7.2, 50 MM AMMONIUM SULFATE, 5% GLYCEROL, 5% SUCROSE

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.977 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 31, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.977 Å / Relative weight: 1
ReflectionResolution: 3.14→50 Å / Num. obs: 53976 / % possible obs: 98 % / Redundancy: 5.5 % / Biso Wilson estimate: 56.82 Å2 / CC1/2: 0.914 / Rmerge(I) obs: 0.088 / Rpim(I) all: 0.039 / Rrim(I) all: 0.097 / Net I/σ(I): 16.2
Reflection shellResolution: 3.14→3.2 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.499 / Mean I/σ(I) obs: 1.5 / Num. unique obs: 2194 / CC1/2: 0.574 / Rpim(I) all: 0.305 / Rrim(I) all: 0.59 / % possible all: 79.8

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Processing

Software
NameVersionClassification
PHENIX1.18.2_3874refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5D3G

5d3g


Resolution: 3.14→34.13 Å / SU ML: 0.2989 / Cross valid method: FREE R-VALUE / σ(F): 1.41 / Phase error: 23.9301
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2437 1998 4.51 %
Rwork0.1939 42330 -
obs0.1962 44328 80.32 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 77.46 Å2
Refinement stepCycle: LAST / Resolution: 3.14→34.13 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15836 1442 186 150 17614
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002418075
X-RAY DIFFRACTIONf_angle_d0.582224851
X-RAY DIFFRACTIONf_chiral_restr0.07852702
X-RAY DIFFRACTIONf_plane_restr0.00332871
X-RAY DIFFRACTIONf_dihedral_angle_d16.8026941
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.14-3.220.3105360.2439758X-RAY DIFFRACTION20.12
3.22-3.310.2535500.23221072X-RAY DIFFRACTION28.64
3.31-3.40.2754680.23231445X-RAY DIFFRACTION38.95
3.4-3.510.26651030.23322174X-RAY DIFFRACTION57.91
3.51-3.640.29611460.22753100X-RAY DIFFRACTION82.74
3.64-3.780.26221720.20063659X-RAY DIFFRACTION97.41
3.78-3.950.2431790.19533764X-RAY DIFFRACTION99.6
3.95-4.160.22951740.17453713X-RAY DIFFRACTION99.92
4.16-4.420.2031790.17133781X-RAY DIFFRACTION99.92
4.42-4.760.19361770.16673742X-RAY DIFFRACTION99.97
4.76-5.240.22591770.17433768X-RAY DIFFRACTION99.95
5.24-60.26671790.20273773X-RAY DIFFRACTION99.9
6-7.540.27721780.22413784X-RAY DIFFRACTION99.92
7.54-34.130.2431800.18623797X-RAY DIFFRACTION98.49
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.1597574754-0.6824437406780.7710425109082.1219915951-0.02398002300823.39891384305-0.05657963094611.145808288260.812295168857-1.37181982862-0.123320675108-0.185664187653-0.6709190761990.1592572772820.2059600322771.37054273618-0.3413738874430.1019852334010.8000813862240.07478721660810.63583651481616.536683932922.5902788626164.191299136
22.848747982511.36373627502-0.6602049254632.81284090814-0.3472106463652.536724537890.02684571658760.1388452185560.657736012297-0.459055184810.182195352450.00727332856117-0.7158377738650.0240974560755-0.1305698230960.550817907005-0.130616105551-0.04208135942280.269204692611-0.1586947843750.60021788772712.288828568927.8711491951189.449304309
30.2677300340710.00805023478573-0.0976329999720.459533570136-0.2314986810090.79115977874-0.0642890394978-0.1067927857890.1340790800230.0537216380175-0.0354873517372-0.0229230751076-0.3283316703740.031946553701-0.02454721993020.144700688707-0.124734664866-0.02711090457980.147445187919-0.407505703250.3133142976028.790225436938.61719591307204.051341787
40.545374317565-0.0125060163256-0.1272855601860.3206501164730.02831641483380.302343578865-0.0159390830943-0.02570959396780.0136899449341-0.01728954422480.09420799363530.0749512689967-0.0478189453313-0.05410358177230.00699964343699-0.1920614627290.1490569790850.02958000904970.398047083879-0.06520864447460.422915671209-21.4539970982-4.75695722603210.384244503
52.82762643231-0.495973966797-0.1582309595270.817820214317-0.5940854654112.16893231433-0.04138761956460.224587205815-0.372406224889-0.05636766692440.073187942350.2218608080930.164028003-0.445313927115-0.01610345632280.05709110483280.003879247303560.07723688029010.256269171688-0.1621643596470.480805600229-17.4692191091-5.37368820366208.314022292
61.27816143502-1.151417187180.5403177491452.26739266781-0.2375247073133.076540058780.07769658447880.403572322173-0.281897245319-0.6825949709230.0870358950041-0.1531450411220.1033702316470.333662895773-0.2401686300830.498106639395-0.2066945799640.1846033201470.475456567185-0.3616732463420.50405443447721.5566965469-4.75351420149177.300813422
70.0134004058133-0.04988401067120.001226300187393.77477927529-1.259754171420.437460290967-0.06243964516570.230415269034-0.253997543595-0.753672904682-0.002007651846130.1494413885150.3894526377470.30668488623-0.03325532057780.698977293594-0.1657805418660.2703042853030.639231197758-0.4291868500830.74279650180221.4091106361-14.4871635798174.445249069
87.15642079988-3.51854031496-2.364482773213.182313648391.842095673623.4852895635-0.136979648717-0.740319568703-0.00572716846915-0.3984963530250.34064506327-0.9030498664390.07159939165791.3099751472-0.1679277832360.6200403495130.1254606245370.08138824978660.776052892258-0.3728023623570.92947904670322.8774595954-29.0013423373189.517610871
91.24459383761-1.52703939847-1.762672855191.975953866422.752150518615.74479163823-0.7167579940270.00504268559899-0.8370822555220.433574645584-0.1008327596760.6964451695361.32774020701-0.614809274550.9256725622250.485554301115-0.01331482539020.2172596319460.378108890946-0.2083479962870.876322994969-13.1452838871-29.062005419204.195476753
100.5697091407080.6122912924350.4275620156171.677388146670.7850903942631.12446646956-0.10398225624-0.011505212089-0.0580517511995-0.117468180423-0.0131988813020.1232036716040.135428033326-0.137941442828-0.01135248413910.15477272698-0.0924049502255-0.04800975594740.240915027309-0.3637627932170.4289246493656.86809615543-15.3127782419195.131968856
110.9664737047190.0869281954245-0.9049629484880.908841309430.3093793189681.016663589920.01183602212531.30704746744-0.155608241254-1.189556933170.09613466175770.296179901837-0.0580929107352-0.421923599511-0.07743903329542.314401178640.37582293094-0.1601066546291.58082403556-0.1584481919690.6703786364021.4689033939-53.4406838932122.609050676
123.12623871640.412208795232-2.792993585151.21868172383-0.8158741361425.487458759110.3507078907710.9899227804850.510430222355-0.896487672631-0.1198438003070.0939639959122-0.398385029306-0.467089059764-0.2276652193491.320262576370.3176161197820.02642293509980.8021125658420.1208554008960.5339093776780.540913627364-37.6707604267139.804133958
131.030290598070.455691895695-0.2458791212521.716995459890.1238267954890.918642154980.0576997764409-0.04063536871490.104159880001-0.516729875409-0.01383218378220.111944964851-0.332517443294-0.191384972436-0.17984657174-0.240697051083-0.178091831424-0.003512420437110.0257138774685-0.08070031181530.101761500568-5.24465246752-52.5728553248175.008185059
143.731024748240.321186333993-2.249449057782.075481828370.7485591673225.339698830220.08383505176980.412559762957-0.048974751905-0.5642762780640.242435888254-0.6185768308650.08345682956810.794537355252-0.331279752130.9433305349950.2442813575890.3143315689040.9185252719610.06592816581450.52185358575723.5083669542-56.8628626206147.67129722
152.02447000261-1.789723704081.275385785292.37060544436-1.30375975850.842942886480.962240706351.61367180657-1.39389435772-0.6637227859490.275255819936-0.3738191526551.158705908232.21838645989-1.235230451951.191138380050.6948127074870.3009264952281.935475246060.1022164314551.449995049732.9680608433-76.5834388427158.661757109
160.855135788605-0.165179258251-0.1714050345020.510783629463-0.02843570928910.043460334823-0.0744744221913-0.158667995133-0.341974410003-0.297916601327-0.0623594884343-0.3361913154260.221002918290.620828981520.1533436702161.092606650490.5064762046320.4176897509431.464864186170.1792868524720.6939703035532.8857053139-63.1477580748149.879320615
171.948651283420.156712840746-1.049654640670.7717985429110.3888461980680.8576015071080.07968237095730.6782616840310.367121540206-0.607867218740.0473482559616-0.615801453688-0.4081793274310.52537290434-0.2093161545950.5198677946650.01899173889840.3042000351661.305866753530.1893613455870.9336843085230.3435494141-65.4481602403176.043918141
183.09259084886-0.4740793716-1.616407111961.81462254029-0.5031833907463.870651270930.09012447529940.0663948556896-0.381553725005-0.28571660536-0.343828572565-0.1837696376710.3029079095320.4147407545130.2833053479790.07998074568130.02953813679360.07302670076290.4077233506950.0868867345040.27762053104314.4035477835-73.3927701718185.071859293
192.97783558137-2.046129951460.2234083878574.760497493110.8084065655381.26727425836-0.0160368873459-0.293076925309-0.140847066319-0.121462986574-0.235527536584-0.08863400537430.3129243304520.4256548845970.2089244654740.32492958480.1342219992290.1876516294820.5469623876770.1153659278040.41467595725117.0877582637-61.734185796168.268684429
202.758129777160.639691350971.300046905090.6065524603011.093252096641.97790150699-0.1195551590211.0381868185-0.788245179759-0.810904757955-0.1580648341670.1104925039060.996908415801-0.6934683034880.2765225805721.29143756491-0.000147073706107-0.1017510956440.80194223874-0.2043446825140.544264810499-5.04075055053-55.4235833909146.535130915
213.80139790659-1.28023266956-3.246805277543.0268495116-1.029789940884.51001800922-0.439263895672-2.089720338061.259482712921.519943077210.0218991864220.519566011744-2.5537611937-0.7183300569860.410669145061.190903102630.1919288963640.06261601533021.11482875966-0.3608681998151.04604869605-0.956575880394-72.8920642747163.688117977
223.6751466702-2.42845827523-5.154315262842.811584930233.654318870287.92816732773-0.474447000721-0.177122224891-2.061744289480.4219524232860.287439463807-1.850699149042.193512648571.429672280830.1883559365430.850224875550.1193098706530.03022723302920.53583074051-0.1252811049080.828964104842-7.16473216337-73.6848855079169.537476279
230.736192773117-1.374163644512.439173767282.7033038553-4.826885478168.627115073710.2490109825970.571448073635-0.272935140974-1.70931819159-0.3981967421150.195815719680.961380057088-0.1992834437650.1061037210111.440455305220.0519048164833-0.1356716877940.872959248577-0.06473087538380.542710537261-4.95111865169-54.3218523614147.217559595
242.27413800878-0.81874392341.07935684472.873587526261.471777888781.85371076715-0.247894479460.914772186078-0.00566189864287-0.9045569662860.1452054983860.9064812679780.366850479393-0.9082611406790.1152021350780.674178615182-0.279271241441-0.1675564300760.7124452074740.02090379497310.5693398849750.70527072406415.4899079044180.877912474
253.67954085433-1.1450426963-0.7153351261774.197240219893.49226777179.00284966191-0.2620219295810.316496513054-2.563775925-0.4727923818290.1565887352750.4456216526622.54495744769-1.001044556410.06343120344260.96159208158-0.2941695310720.08329930176510.671561826343-0.08643261480441.11745053092-15.0452113478-4.72829442437188.447893095
262.85700539483.22820154282-0.3410654962714.03075740958-1.474158451693.08201252908-0.9324118298690.317330640828-0.841035178571-1.672272376680.3819967326340.1197169893750.610612831359-0.4827115699110.4948362412130.768548963877-0.258875487180.1072309622180.592652635970.01079077347480.9516351177081.1393698210215.8654944196182.11321492
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 194 )AA1 - 1941 - 194
22chain 'A' and (resid 195 through 325 )AA195 - 325195 - 325
33chain 'A' and (resid 326 through 421 )AA326 - 421326 - 421
44chain 'A' and (resid 422 through 499 )AA422 - 499422 - 499
55chain 'A' and (resid 500 through 553 )AA500 - 553500 - 553
66chain 'B' and (resid 4 through 83 )BE4 - 831 - 80
77chain 'B' and (resid 84 through 167 )BE84 - 16781 - 164
88chain 'B' and (resid 168 through 253 )BE168 - 253165 - 240
99chain 'B' and (resid 254 through 325 )BE254 - 325241 - 312
1010chain 'B' and (resid 326 through 428 )BE326 - 428313 - 415
1111chain 'C' and (resid 1 through 155 )CL1 - 1551 - 155
1212chain 'C' and (resid 156 through 296 )CL156 - 296156 - 296
1313chain 'C' and (resid 297 through 553 )CL297 - 553297 - 553
1414chain 'D' and (resid 6 through 83 )DQ6 - 831 - 78
1515chain 'D' and (resid 84 through 104 )DQ84 - 10479 - 99
1616chain 'D' and (resid 105 through 185 )DQ105 - 185100 - 180
1717chain 'D' and (resid 186 through 269 )DQ186 - 269181 - 252
1818chain 'D' and (resid 270 through 363 )DQ270 - 363253 - 348
1919chain 'D' and (resid 364 through 427 )DQ364 - 427349 - 414
2020chain 'F' and (resid 0 through 11 )FV0 - 11
2121chain 'F' and (resid 12 through 16 )FV12 - 16
2222chain 'F' and (resid 17 through 21 )FV17 - 21
2323chain 'F' and (resid 22 through 33 )FV22 - 33
2424chain 'E' and (resid 0 through 11 )EX0 - 11
2525chain 'E' and (resid 12 through 21 )EX12 - 21
2626chain 'E' and (resid 22 through 33 )EX22 - 33

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