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- PDB-7lri: Structure of HIV-1 Reverse Transcriptase in complex with DNA, dTT... -

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Basic information

Entry
Database: PDB / ID: 7lri
TitleStructure of HIV-1 Reverse Transcriptase in complex with DNA, dTTP, and CA(2+) ion
Components
  • (Reverse transcriptase ...) x 2
  • DNA/RNA (38-MER)
KeywordsTRANSFERASE/DNA / Human immunodeficiency virus 1 / Protein/dsDNA / Aptamer / dNTP / TRANSFERASE / TRANSFERASE-DNA complex
Function / homology
Function and homology information


HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency ...HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / symbiont-mediated suppression of host gene expression / viral penetration into host nucleus / RNA stem-loop binding / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / host cell / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / Hydrolases; Acting on ester bonds / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / symbiont entry into host cell / viral translational frameshifting / lipid binding / host cell nucleus / host cell plasma membrane / structural molecule activity / virion membrane / proteolysis / DNA binding / zinc ion binding / membrane
Similarity search - Function
Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain ...Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain / Integrase, C-terminal, retroviral / Integrase DNA binding domain profile. / Immunodeficiency lentiviral matrix, N-terminal / gag gene protein p17 (matrix protein) / RNase H / Integrase core domain / Integrase, catalytic core / Integrase catalytic domain profile. / Retropepsin-like catalytic domain / RNase H type-1 domain profile. / Ribonuclease H domain / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Retroviral nucleocapsid Gag protein p24, C-terminal domain / Gag protein p24 C-terminal domain / Reverse transcriptase domain / Reverse transcriptase (RT) catalytic domain profile. / Retropepsins / Retroviral aspartyl protease / Aspartyl protease, retroviral-type family profile. / Peptidase A2A, retrovirus, catalytic / Reverse transcriptase (RNA-dependent DNA polymerase) / Retroviral matrix protein / Retrovirus capsid, C-terminal / Retrovirus capsid, N-terminal / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Aspartic peptidase, active site / Eukaryotic and viral aspartyl proteases active site. / Ribonuclease H superfamily / Aspartic peptidase domain superfamily / Ribonuclease H-like superfamily / Reverse transcriptase/Diguanylate cyclase domain / DNA/RNA polymerase superfamily
Similarity search - Domain/homology
THYMIDINE-5'-TRIPHOSPHATE / DNA / DNA (> 10) / Gag-Pol polyprotein
Similarity search - Component
Biological speciesHuman immunodeficiency virus type 1
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.05 Å
AuthorsHoang, A. / Ruiz, F.X. / Arnold, E.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R37 AI027690 United States
CitationJournal: Drug Discov Today / Year: 2022
Title: Structural basis of HIV inhibition by L-nucleosides: Opportunities for drug development and repurposing.
Authors: Ruiz, F.X. / Hoang, A. / Dilmore, C.R. / DeStefano, J.J. / Arnold, E.
History
DepositionFeb 16, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 23, 2022Provider: repository / Type: Initial release
Revision 1.1Mar 9, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.name
Revision 1.2Jul 6, 2022Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title
Revision 1.3Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Reverse transcriptase p66
B: Reverse transcriptase p51
C: Reverse transcriptase p66
D: Reverse transcriptase p51
E: DNA/RNA (38-MER)
F: DNA/RNA (38-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)251,27417
Polymers249,5776
Non-polymers1,69711
Water1,74797
1
A: Reverse transcriptase p66
B: Reverse transcriptase p51
E: DNA/RNA (38-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,86711
Polymers124,7893
Non-polymers1,0798
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: Reverse transcriptase p66
D: Reverse transcriptase p51
F: DNA/RNA (38-MER)
hetero molecules


Theoretical massNumber of molelcules
Total (without water)125,4076
Polymers124,7893
Non-polymers6183
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)89.423, 131.073, 129.847
Angle α, β, γ (deg.)90.000, 101.549, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Reverse transcriptase ... , 2 types, 4 molecules ACBD

#1: Protein Reverse transcriptase p66


Mass: 63874.133 Da / Num. of mol.: 2 / Mutation: C280S, D498N
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 / Production host: Escherichia coli (E. coli)
References: UniProt: P03366, RNA-directed DNA polymerase, DNA-directed DNA polymerase, retroviral ribonuclease H
#2: Protein Reverse transcriptase p51


Mass: 50096.539 Da / Num. of mol.: 2 / Mutation: C280S
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 / Production host: Escherichia coli (E. coli) / References: UniProt: P03366

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DNA chain , 1 types, 2 molecules EF

#3: DNA chain DNA/RNA (38-MER)


Mass: 10817.920 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Non-polymers , 5 types, 108 molecules

#4: Chemical ChemComp-TTP / THYMIDINE-5'-TRIPHOSPHATE


Mass: 482.168 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H17N2O14P3 / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca
#6: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C3H8O3
#7: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 97 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.99 Å3/Da / Density % sol: 58.83 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.2
Details: 10-12% PEG 8000, 50 MM BISTRIS-PROPANE PH 7.2, 50 MM AMMONIUM SULFATE, 5% GLYCEROL, 5% SUCROSE

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.977 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 25, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.977 Å / Relative weight: 1
ReflectionResolution: 3.05→50 Å / Num. obs: 55508 / % possible obs: 99.1 % / Redundancy: 6.6 % / Biso Wilson estimate: 77.96 Å2 / CC1/2: 0.912 / Rmerge(I) obs: 0.121 / Rpim(I) all: 0.052 / Rrim(I) all: 0.127 / Net I/σ(I): 14.22
Reflection shellResolution: 3.05→3.1 Å / Redundancy: 5.3 % / Rmerge(I) obs: 0.01 / Mean I/σ(I) obs: 1.44 / Num. unique obs: 2697 / CC1/2: 0.597 / Rpim(I) all: 0.464 / Rrim(I) all: 0.01 / % possible all: 96.5

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Processing

Software
NameVersionClassification
PHENIX1.18.2_3874refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5D3G

5d3g


Resolution: 3.05→47.18 Å / SU ML: 0.4255 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 26.1829
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2436 1999 3.61 %
Rwork0.2107 53428 -
obs0.2119 55427 98.87 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 102.47 Å2
Refinement stepCycle: LAST / Resolution: 3.05→47.18 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15818 1440 100 97 17455
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002817951
X-RAY DIFFRACTIONf_angle_d0.566424689
X-RAY DIFFRACTIONf_chiral_restr0.04342684
X-RAY DIFFRACTIONf_plane_restr0.0042852
X-RAY DIFFRACTIONf_dihedral_angle_d19.35986860
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.05-3.120.35791380.3213671X-RAY DIFFRACTION95.54
3.12-3.210.33141410.29723773X-RAY DIFFRACTION98.49
3.21-3.30.3251420.28273825X-RAY DIFFRACTION98.76
3.3-3.410.29981410.26863752X-RAY DIFFRACTION98.53
3.41-3.530.32491430.24753811X-RAY DIFFRACTION99.1
3.53-3.670.25491420.23123801X-RAY DIFFRACTION99.05
3.67-3.840.22461430.22223837X-RAY DIFFRACTION99.23
3.84-4.040.21431430.20653810X-RAY DIFFRACTION99.32
4.04-4.30.22221440.18883843X-RAY DIFFRACTION99.35
4.3-4.630.21761420.17623825X-RAY DIFFRACTION99.32
4.63-5.090.21471440.18263834X-RAY DIFFRACTION99.52
5.09-5.830.25021450.19923874X-RAY DIFFRACTION99.65
5.83-7.340.24451440.21743866X-RAY DIFFRACTION99.78
7.34-47.180.21361470.18183906X-RAY DIFFRACTION98.61
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.70107301693-3.050131899570.839317553185.55751370894-2.861959398036.60580447324-0.01199342954010.896353839570.318597752531-1.18378140193-0.100466429539-0.108340781341-0.651655282504-0.1258621863030.08216623305151.64283231408-0.209555692208-0.08704281016210.7785954141160.1276492089610.6203072436941.8753305011226.1810123135143.496185758
22.191569223960.0290514098171-0.4928505917762.480792188560.03401582637222.11678967460.0428012293136-0.1310057935670.577042924885-0.9198831452760.215266035745-0.126471634011-0.5908062716980.266120792536-0.2196160616720.980422815128-0.1987749686340.09118748190850.507792111497-0.0862933154490.7878192084917.9229694531325.0654206542165.810968062
35.836307667441.30710402479-2.178344148741.47780154533-0.8888829386641.6239808579-0.0819331487173-0.6259265999330.0449387552565-0.009632336206670.06579435161360.147192291752-0.1096807164260.09999120860940.01056969047340.3166477924530.0112199265646-0.01317634721960.658907391381-0.08059865802890.467554096056-13.39079458733.79719178819192.906970558
42.72161422832-2.95957003834-2.964634343695.54722556312.39352476283.98208389373-0.0430077454079-0.5383236664-0.397904804992-0.415069451909-0.4712070308010.9859705952440.161370418637-0.574956131680.5337919313320.4273548269550.0231442696718-0.002002713150590.7482663527220.04937618715950.921670101064-29.2988311922-5.82640126339196.685875818
54.91261681417-0.5699451095161.053104175572.76945382761-0.5502241607866.85643544176-0.07525612007990.0241481948054-0.16646412391-0.5679129929730.150172716478-0.362837962367-0.2081654768290.604978496315-0.08652314437090.552270778972-0.1126917770970.1897638611960.378047103745-0.08075559807020.6383778844511.876359154-4.35093879451162.950325519
61.628246057440.734902641591-0.4280858516976.47145762788-0.5478667500861.81051868548-0.2626374522780.171587120013-0.397978859233-1.048438331960.32058825472-0.2804006599190.4007600383920.444506006154-0.08288500611660.779797677443-0.08439626208780.2110725967690.587355903643-0.150315905690.83232809368711.9938020592-15.6788819317160.427091206
73.06908324009-3.05631088154-0.2881613272494.253551901512.011347689282.35399934463-0.384271515988-0.835239045156-0.42328880931-0.4212111348020.201189432456-0.9218935145760.7000405185771.599563932190.2434256725030.5955066947180.23191520280.07390586972321.034844839110.1040809867880.94973701724611.0635426364-28.4648905623176.956119749
83.995640409290.102432022572-3.666569425990.7735057442691.00104052754.13286362167-0.454304470194-0.0883087971523-1.114096049650.15702090179-0.1655569339230.4589168657360.673519258643-0.4762247831870.7687707026330.69008302270.0663694287190.2768209345030.7347973626160.06376702575621.15698306653-22.6203228524-27.5963932994191.126415003
94.346804920141.339013552131.425783810616.52874137943.859729202545.17535967711-0.162592036386-0.470015537595-0.372210308540.00172894893142-0.1402941075590.4501366360650.335709605392-0.04174042399870.2487469856830.3147680450670.05734736990150.05491219697050.4476936376290.07311270801520.647639586906-2.62663084633-14.4084348158181.194857473
102.687950813130.759332205573-1.310974617275.47936823529-0.412805976068.53200340491-0.3212155287751.64734800076-0.0544297791753-0.6410609978670.5359235090271.246768860730.0889893263094-1.84871621265-0.2030445896061.45684742069-0.0124722599277-0.3149511820532.12376165292-0.1154285803420.924493975422-15.9013114846-55.108731461106.274616411
113.02772625505-1.9445691713-0.8555067440423.97668885405-2.115587584552.511423608280.5256547574291.64339846704-0.114847389921-1.10633018603-0.07974348721741.1567962860.0723677139212-1.11053008078-0.4167752286871.219863445240.163733935636-0.05477541364621.701579974530.1486269827740.85343583386-9.27018714204-49.5170937233113.09472866
124.266423504850.114961439611-1.606179860976.24951106301-1.941508080071.356811605620.813523502752.018632939190.919525089492-1.17363422721-0.2894980433750.137795333217-0.974240207749-0.603881281482-0.2212700950371.683906527480.4662092201180.2869156332841.702925253750.5051360721780.866319049203-0.466542589533-37.9951958726115.447527328
135.75980054082.06438551099-1.495382076284.02164220962-1.015169506513.818759244040.6527789019771.057481045831.22356642362-0.334101847102-0.2150987675930.751584523491-0.894585876739-1.3755890122-0.4445771963660.9194832653330.4465249337370.152687294371.302424507850.2185675262081.05204814109-23.594960704-37.4210041933137.335630085
143.94496461723-0.566951430621-2.257814657754.47480546385-2.340941726438.409331500050.08485388461840.07757520066820.4827917920740.06090988481880.0748557240865-0.350798642575-0.671027979251-0.296698837605-0.1050041814890.436707571786-0.0399011498960.08417468018350.337662665742-0.05774027039080.643788748341-9.4472461566-41.7963678135153.61334625
153.33740757533-0.114701523914-0.04749284442062.8024945562-0.4767692648184.22987375408-0.102835925905-0.143915955063-0.0149519002102-0.1225916680990.1329622061130.1681834263740.0948819331585-0.354254734714-0.03267656241310.220165355431-0.05068374618-0.005551970972160.5735826981590.01221441194670.522204447015-24.6401988817-62.9418014278171.607561647
166.486363258450.274781468421-0.3422467203482.48870265542-1.490194826785.650302663170.0704374196004-0.189558755569-0.5767410170450.0416266207526-0.1144432090640.3991488539430.370168047396-0.421795954664-0.02267507914470.312623282979-0.0107154056838-0.006947413585460.6653110715640.01130424944730.621252088311-23.1937386355-65.0495391733173.97932366
177.5959555482-0.346164426844-0.9951798642296.577259663641.281639599327.3669294052-0.0221928420050.405673435304-0.188181350855-0.4402155979670.125277637473-0.7077964313270.5943311765150.534630904327-0.1575461958390.6435514081460.09680747786580.1776138277170.618456523090.03597775589760.5754519610810.2484790669-60.0157348445133.842884809
181.16433503705-0.1881213221130.5659868839451.40135700987-1.548822482324.465556989550.10640853852-0.0741683859629-0.0661533764011-0.375871297491-0.10340576821-0.4114068298480.4557761533761.21407979933-0.0063884968520.7830656319420.2072977832530.2801868664341.138176916940.05711982883490.83901065055222.5202621494-66.0227510198140.793429575
195.47674254290.930417138801-4.816559095290.848769609007-1.506554330847.38321798052-1.13747837492-0.9451199906350.162942849736-0.4990046621250.256126514203-0.3200471931741.918540451631.87612055640.6416869862490.682380329824-0.03385487047360.1437567257540.980690245060.008753140198250.8782203808713.1024946414-65.9154538515167.661471777
203.34328505627-0.0408630043617-1.105171236222.05010535542-2.072064960364.56558934060.0756586179154-0.257833006418-0.422545686103-0.278735964762-0.190920639828-0.0187030293020.3497316962460.1056091048040.2201094449770.381475040834-0.0306356161355-0.02221459243680.499810327583-0.008955478912370.601068302368-0.733725581142-72.8241927569172.772452331
216.38698418231-3.849980581820.7262635556158.14298310836-1.461595902377.87573513125-0.273613964082-0.0847990383603-0.4594255884870.100221822129-0.1426412685810.3324363347530.4503957840640.05782054735510.406856404010.415889486034-0.06098438209150.08408591166660.391494479354-0.02370201663820.5221518489283.82934098472-61.9781921736154.983250034
227.40189894724-3.46504782723-3.768819057362.308751441240.2807845086819.32383842404-0.1373649097180.0631185405110.869832059284-1.189220130090.1960259817840.706225864107-0.557278943027-0.078136452058-0.07182533888090.999479341006-0.20653489422-0.2548668474050.417593898618-0.02532260102250.911273769039-7.001244738218.5202233521165.697734801
234.97356418373-4.35748990887-5.776370248884.461639512545.507948253047.0284431798-1.715028169650.276205999737-2.11186504813-0.898849007514-0.1311324595230.439250551551.44766510731-0.4291979041741.651097044751.38888025127-0.3630477326020.09128660946151.14888628668-0.0106439544921.12602815108-23.8040196072-2.83600843906173.839472273
244.616559601316.25638893896-2.294213030438.72316147944-1.767978640945.00688471208-1.17512146095-0.118898137862-0.34962130318-2.762325944430.44900595747-0.06390024883730.506298797942-0.4780698965520.871102200811.09425580415-0.1259399877740.04822711920090.7068922887620.1077462885561.04446144086-8.420725586215.6801709108169.111976486
254.769279591022.16336359825-1.021487469894.881229128770.1293527143590.229301627908-0.1702865335441.47222090240.456766051495-0.2215277672810.8582629289040.8063096389560.3441819972750.0586821963175-0.6132870546890.7747166665720.103319940135-0.06442637238541.421423464270.008429132917760.792256227051-18.1375870799-58.8980350034135.960763083
263.16649848423-1.33521814517-3.592583196934.313724563476.140667136149.788054822281.26929439640.0761822174139-1.416191423964.78115904878-2.56368587868-5.625331314081.836507456932.183399668750.7744671814861.23779158765-0.105741181587-0.4959133231671.238720685280.2706451259551.98587559423-19.5944640215-73.3232141274157.084037371
272.96078866428-3.578809960750.4916664454847.26321003169-1.11265407672.372791391590.9579398641871.26528107634-0.160057073765-1.24203034806-0.8777040353171.07009246050.4985788142230.5020459184340.1027967229860.973043013886-0.24865892463-0.05862656897941.549974641050.121308787790.930763878529-18.3556407413-54.1718364441135.497369723
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 83 )AA1 - 831 - 83
22chain 'A' and (resid 84 through 325 )AA84 - 32584 - 325
33chain 'A' and (resid 326 through 515 )AA326 - 515326 - 515
44chain 'A' and (resid 516 through 554 )AA516 - 554516 - 554
55chain 'B' and (resid 4 through 83 )BC4 - 831 - 80
66chain 'B' and (resid 84 through 174 )BC84 - 17481 - 171
77chain 'B' and (resid 175 through 253 )BC175 - 253172 - 237
88chain 'B' and (resid 254 through 325 )BC254 - 325238 - 309
99chain 'B' and (resid 326 through 428 )BC326 - 428310 - 412
1010chain 'C' and (resid 1 through 83 )CD1 - 831 - 83
1111chain 'C' and (resid 84 through 143 )CD84 - 14384 - 143
1212chain 'C' and (resid 144 through 226 )CD144 - 226144 - 226
1313chain 'C' and (resid 227 through 325 )CD227 - 325227 - 325
1414chain 'C' and (resid 326 through 421 )CD326 - 421326 - 421
1515chain 'C' and (resid 422 through 515 )CD422 - 515422 - 515
1616chain 'C' and (resid 516 through 554 )CD516 - 554516 - 554
1717chain 'D' and (resid 4 through 90 )DE4 - 901 - 87
1818chain 'D' and (resid 91 through 212 )DE91 - 21288 - 209
1919chain 'D' and (resid 213 through 253 )DE213 - 253210 - 237
2020chain 'D' and (resid 254 through 363 )DE254 - 363238 - 347
2121chain 'D' and (resid 364 through 428 )DE364 - 428348 - 412
2222chain 'E' and (resid 0 through 11 )EF0 - 11
2323chain 'E' and (resid 12 through 21 )EF12 - 21
2424chain 'E' and (resid 22 through 34 )EF22 - 34
2525chain 'F' and (resid 0 through 16 )FG0 - 16
2626chain 'F' and (resid 17 through 21 )FG17 - 21
2727chain 'F' and (resid 22 through 34 )FG22 - 34

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