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- PDB-7c7i: Crystal structure of SHANK3 SPN domain in complex with GTP-bound ... -

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Basic information

Entry
Database: PDB / ID: 7c7i
TitleCrystal structure of SHANK3 SPN domain in complex with GTP-bound Rap1b(E30D,K31E)
Components
  • Ras-related protein Rap-1b
  • SH3 and multiple ankyrin repeat domains protein 3
KeywordsPROTEIN BINDING / SHANK3 / Rap1b / SPN / Ras
Function / homology
Function and homology information


guanylate kinase-associated protein clustering / striatal medium spiny neuron differentiation / synaptic receptor adaptor activity / positive regulation of glutamate receptor signaling pathway / postsynaptic density assembly / Rap protein signal transduction / NMDA glutamate receptor clustering / positive regulation of synapse structural plasticity / modification of postsynaptic structure / negative regulation of actin filament bundle assembly ...guanylate kinase-associated protein clustering / striatal medium spiny neuron differentiation / synaptic receptor adaptor activity / positive regulation of glutamate receptor signaling pathway / postsynaptic density assembly / Rap protein signal transduction / NMDA glutamate receptor clustering / positive regulation of synapse structural plasticity / modification of postsynaptic structure / negative regulation of actin filament bundle assembly / vocal learning / negative regulation of cell volume / regulation of cell junction assembly / positive regulation of long-term neuronal synaptic plasticity / AMPA glutamate receptor clustering / negative regulation of calcium ion-dependent exocytosis / positive regulation of integrin activation / vocalization behavior / negative regulation of synaptic vesicle exocytosis / neuron spine / calcium-ion regulated exocytosis / regulation of dendritic spine morphogenesis / dendritic spine morphogenesis / Rap1 signalling / brain morphogenesis / Neurexins and neuroligins / establishment of endothelial barrier / regulation of establishment of cell polarity / regulation of long-term synaptic potentiation / MET activates RAP1 and RAC1 / positive regulation of AMPA receptor activity / positive regulation of dendritic spine development / ciliary membrane / azurophil granule membrane / regulation of long-term synaptic depression / adult behavior / p130Cas linkage to MAPK signaling for integrins / social behavior / positive regulation of excitatory postsynaptic potential / GRB2:SOS provides linkage to MAPK signaling for Integrins / RET signaling / cellular response to cAMP / synapse assembly / ionotropic glutamate receptor binding / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / lipid droplet / Integrin signaling / positive regulation of synaptic transmission, glutamatergic / small monomeric GTPase / G protein activity / learning / positive regulation of long-term synaptic potentiation / establishment of localization in cell / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / memory / SH3 domain binding / GDP binding / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / : / MAPK cascade / cell-cell junction / Signaling by BRAF and RAF1 fusions / actin binding / scaffold protein binding / cell population proliferation / dendritic spine / postsynaptic density / positive regulation of ERK1 and ERK2 cascade / neuron projection / GTPase activity / glutamatergic synapse / Neutrophil degranulation / protein-containing complex binding / GTP binding / extracellular exosome / zinc ion binding / membrane / plasma membrane / cytosol
Similarity search - Function
Ras-related protein Rap1 / PDZ domain 6 / PDZ domain / Variant SH3 domain / SAM domain (Sterile alpha motif) / Small GTPase, Ras-type / small GTPase Ras family profile. / SAM domain profile. / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Sterile alpha motif. ...Ras-related protein Rap1 / PDZ domain 6 / PDZ domain / Variant SH3 domain / SAM domain (Sterile alpha motif) / Small GTPase, Ras-type / small GTPase Ras family profile. / SAM domain profile. / Ran (Ras-related nuclear proteins) /TC4 subfamily of small GTPases / Sterile alpha motif. / Sterile alpha motif domain / Sterile alpha motif/pointed domain superfamily / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / PDZ domain profile. / Domain present in PSD-95, Dlg, and ZO-1/2. / PDZ domain / Rab subfamily of small GTPases / Ankyrin repeats (3 copies) / PDZ superfamily / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Ankyrin repeat-containing domain superfamily / Src homology 3 domains / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Small GTP-binding protein domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
GUANOSINE-5'-TRIPHOSPHATE / Ras-related protein Rap-1b / SH3 and multiple ankyrin repeat domains protein 3
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.28 Å
AuthorsXiao, L.X. / Pan, L.F.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)21822705 China
Citation
Journal: Chin.J.Chem. / Year: 2020
Title: Mechanistic Insights into the Interactions of Ras Subfamily GTPases with the SPN Domain of Autism-associated SHANK3.
Authors: Xu, X.L. / Liu, J.P. / Wang, Y.L. / Wang, Y. / Gong, X. / Pan, L.F.
#1: Journal: Acta Crystallogr. D Biol. Crystallogr. / Year: 2012
Title: Towards automated crystallographic structure refinement with phenix.refine.
Authors: Afonine, P.V. / Grosse-Kunstleve, R.W. / Echols, N. / Headd, J.J. / Moriarty, N.W. / Mustyakimov, M. / Terwilliger, T.C. / Urzhumtsev, A. / Zwart, P.H. / Adams, P.D.
#2: Journal: Acta Crystallogr. D Biol. Crystallogr. / Year: 2010
Title: PHENIX: a comprehensive Python-based system for macromolecular structure solution.
Authors: Adams, P.D. / Afonine, P.V. / Bunkoczi, G. / Chen, V.B. / Davis, I.W. / Echols, N. / Headd, J.J. / Hung, L.W. / Kapral, G.J. / Grosse-Kunstleve, R.W. / McCoy, A.J. / Moriarty, N.W. / ...Authors: Adams, P.D. / Afonine, P.V. / Bunkoczi, G. / Chen, V.B. / Davis, I.W. / Echols, N. / Headd, J.J. / Hung, L.W. / Kapral, G.J. / Grosse-Kunstleve, R.W. / McCoy, A.J. / Moriarty, N.W. / Oeffner, R. / Read, R.J. / Richardson, D.C. / Richardson, J.S. / Terwilliger, T.C. / Zwart, P.H.
History
DepositionMay 25, 2020Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0May 5, 2021Provider: repository / Type: Initial release
Revision 1.1Nov 29, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ras-related protein Rap-1b
B: Ras-related protein Rap-1b
C: SH3 and multiple ankyrin repeat domains protein 3
D: SH3 and multiple ankyrin repeat domains protein 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)61,5369
Polymers60,4014
Non-polymers1,1355
Water3,387188
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: equilibrium centrifugation
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)48.502, 55.619, 189.884
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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Protein , 2 types, 4 molecules ABCD

#1: Protein Ras-related protein Rap-1b / GTP-binding protein smg p21B


Mass: 19006.416 Da / Num. of mol.: 2 / Mutation: E30D,K31E
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: RAP1B, OK/SW-cl.11 / Production host: Escherichia coli (E. coli) / References: UniProt: P61224
#2: Protein SH3 and multiple ankyrin repeat domains protein 3 / Shank3 / Proline-rich synapse-associated protein 2 / ProSAP2


Mass: 11193.857 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BYB0

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Non-polymers , 4 types, 193 molecules

#3: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE / Guanosine triphosphate


Mass: 523.180 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: C10H16N5O14P3 / Feature type: SUBJECT OF INVESTIGATION / Comment: GTP, energy-carrying molecule*YM
#4: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: Ca
#5: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#6: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 188 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.22 Å3/Da / Density % sol: 44.51 %
Crystal growTemperature: 289 K / Method: vapor diffusion, sitting drop
Details: 14.4% (w/v) PEG 8000, Sodium cacodylate/Hydrochloric acid (pH 6.5), 160 mM Calcium acetate, 20% (v/v) Glycerol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.97891 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 5, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97891 Å / Relative weight: 1
ReflectionResolution: 2.28→50 Å / Num. obs: 23777 / % possible obs: 98.4 % / Redundancy: 8.8 % / Biso Wilson estimate: 31.93 Å2 / Rmerge(I) obs: 0.153 / Net I/σ(I): 13.9
Reflection shellResolution: 2.3→2.34 Å / Rmerge(I) obs: 0.835 / Num. unique obs: 1181

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Processing

Software
NameVersionClassification
PHENIX1.15.2_3472refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5G4X, 4DXA

5g4x

4dxa


Resolution: 2.28→29.9 Å / SU ML: 0.2591 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 24.6199
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2465 1125 4.78 %
Rwork0.1716 22430 -
obs0.1752 23555 96.6 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 37.51 Å2
Refinement stepCycle: LAST / Resolution: 2.28→29.9 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4049 0 67 188 4304
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00724183
X-RAY DIFFRACTIONf_angle_d0.97045671
X-RAY DIFFRACTIONf_chiral_restr0.0548629
X-RAY DIFFRACTIONf_plane_restr0.005735
X-RAY DIFFRACTIONf_dihedral_angle_d10.49862546
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.28-2.380.28921180.20792466X-RAY DIFFRACTION86.05
2.38-2.510.30181450.21182774X-RAY DIFFRACTION97.85
2.51-2.660.29841300.19732805X-RAY DIFFRACTION98
2.66-2.870.29851510.19412782X-RAY DIFFRACTION97.41
2.87-3.160.27441510.18672830X-RAY DIFFRACTION99
3.16-3.610.25031360.16412861X-RAY DIFFRACTION98.26
3.61-4.550.19781350.13512873X-RAY DIFFRACTION97.47
4.55-29.90.21751590.17133039X-RAY DIFFRACTION98.61
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.41356974469-0.216568652601-0.6393889160653.33736815684-0.5282219917172.406134658720.0568162271030.213022187072-0.250138563636-0.137790953489-0.004748266391960.145648141310.0272443999691-0.201200989167-0.05894977676010.17097303632-0.014914605345-0.02901040184760.23832130084-0.03707464026250.1761248713130.402853593416-7.28719804536-20.4947089126
28.203232067371.03121899121.381547724831.363002052450.8261656830263.0287816856-0.09165113997430.186212837086-0.771910398259-0.2168782637040.0530339640358-0.0610895321467-0.09000624037030.1092139129240.02329284634010.2512055046170.0307281847002-0.01335201809580.213635279076-0.03045171329420.2514953644949.15832573359-12.4317742-23.4357123049
33.77754253752-1.134748073015.270232751832.57132955391-3.112981682939.290832205340.093152398214-0.943743734913-1.267049403680.6139070638310.3668789939130.5124393472960.123145021889-1.04229452712-0.4781527423970.452554160159-0.05280267201010.05964655968730.3909932121640.02048258800650.475887712684-6.07423328525-20.2832485802-15.3603542571
42.04777330836-2.75449916775-0.6342596286447.31330733879-0.01808563171522.54281781103-0.0747438928993-0.0850418235182-0.09426764477890.1549820837010.1076625118480.1179639623080.110409182147-0.15347496618-0.03175091625440.180139938946-0.00511786070801-0.01765031377810.198336567317-0.004871937732160.194285077697-0.0457449757289-11.6248371653-6.46879393166
54.86858964649-2.279641888180.7161908895725.319530603-0.9067507875454.5012173976-0.0865918080861-0.4639458829230.2478375678550.520617755546-0.0309865679388-0.248618118392-0.0827693046054-0.1271251623660.1968040434640.2321722856130.00312022835292-0.001400777954540.27818459379-0.06880342375290.2055775632793.09112033273-2.74491936939-0.817863297239
62.50183786515-1.62793319318-1.341000655935.491156199320.7903583003022.3303990120.03679439827020.0656430281187-0.106800252420.235885417783-0.1421008868750.0723886194794-0.1597811060720.2759101737280.09535593973070.181689969403-0.0426992264094-0.03220323714280.241689772237-0.0314842002460.20719420388410.410573437-5.80285792138-14.3809485825
76.80128771729-1.433798779831.664084444773.448073931-0.4440755016622.68875958408-0.1428801081520.2102086433650.373261828536-0.373740906589-0.112636790229-0.243233237277-0.1321995614530.04880052765430.2616516529170.2495711761020.002035052550940.03012989914090.1556073705020.02190368462070.13635194707120.446112103510.2217107706-23.6233505314
87.724813529896.22066651439-0.331820738145.10412621295-0.02977161027220.562542107089-0.07325727556450.240661372102-0.813812878489-0.1356025689190.458932934333-0.716109719185-0.1995099666120.228445690754-0.3420525371670.198265602161-0.01295232980160.006092589894670.2857549442-0.05338119120230.35173296360930.71847250011.70494630125-23.7425701006
95.697974707660.771451138065-0.7714178220721.69868022220.1910802378293.32951016723-0.1020625156620.5680368536570.314304994846-0.121021059651-0.03118390918680.138269044710.166714707787-0.276047037730.104829565330.2413920713680.006586016147320.01641531311660.2810784319560.03863509909890.25638624939215.040970113711.0060394074-28.3492450914
104.15363364195-1.25255706168-0.09453476737767.20762730518-1.020942463714.598464246260.3188681815850.4147928349811.26780906564-0.08219032318750.0513393025376-0.763878023677-0.9096327848050.6802342576380.2032197540110.562776540987-0.09213794391930.01640319401780.4153744097540.0281788836550.51182820955230.441286084721.6873153835-24.1165365753
113.1868331556-0.9541263877571.112387878123.72892775743-0.543260030864.17631027398-0.182579937014-0.07177481512360.5547721066690.247146980940.0162884569769-0.475929412127-0.6670773221310.1001092499650.07705545210970.254170574929-0.01491125210570.01486876944570.183873548196-0.02175103101450.28994395400424.506015408217.1500397473-12.6691035416
124.83907391723-3.82002620610.08334455711073.62320793467-1.207001933232.16801037059-0.401602261654-0.422060641968-0.1451431699261.127242526580.3859005606570.1872450686260.0808767161727-0.0809878039694-0.02654851303060.4092098274880.0351078245605-0.003228298337530.314799439058-0.05308944724020.28536932242423.47665922849.24004978935-1.87639847954
132.641512589760.3531248861550.4269401213971.52223900352-0.2665801975014.5266736701-0.0239656139718-0.1672429300320.06267582478920.115956848958-0.09204537522610.0138940075477-0.291048338076-0.4273689335330.07370181860080.23668315560.0185468940.01869860518880.2143494962880.01554204912690.18776253190714.669057814210.5498383243-14.7837428063
147.98517777948-2.21567995884-1.05074604564.508574737690.7828820594924.51196371418-0.1544505786490.1194580430960.227204643415-0.0936132719340.0797143986723-0.166095542484-0.2094178001420.09753973074740.0965862845430.209791865230.01542733110480.02329902132860.2306499821930.03871549323930.1359878442132.532280693110.327321001-37.6716338853
156.04708396002-3.75660907507-3.619615320083.836137084671.392407780544.697253114450.1582547998-0.247211098774-0.236837424997-0.179899939027-0.0582302037689-0.0752880896024-0.188715494940.190673556529-0.0799042278250.2040079155140.0060849358156-0.05295707679570.184942855834-0.03108816765260.21083560744836.07256600755.61185544749-38.4176689395
166.584923172522.80949249122-3.332860594322.57500786629-0.2680443207842.74103495525-0.1745114818630.243112923076-1.01249985689-0.0935546990470.2235108435650.2688865952750.717803309282-0.0999697177268-0.05933802066480.3719290748870.0133865015447-0.05061108609380.2893787561160.02511990445590.44542428760927.7058875224-0.900330403061-42.1777678847
170.715513901174-2.237845845-0.1022334074732.00323477311-2.043273233181.83637262439-0.232839362782-0.2495968861790.656868968086-0.4221697682580.167895556029-1.271123050150.6224164691420.380831456779-0.1346056405190.3690960559270.0532749732422-0.0606374718780.279626329854-0.008084050532340.44374432179929.803240349218.8370749472-50.6570450976
186.109394447492.167265276723.770505505626.461739211340.7179511894086.37314912468-0.03608026565910.823029840921-0.370980812552-0.9297008588-0.0795720990462-0.474308000040.01258318483730.9702230762740.1116598865670.2560094834450.06064696160670.06404904786120.3890393757930.00653548075720.22040561275440.30182414238.23609529744-50.0618908935
192.42752383545-1.78829090697-2.737958280161.910919735813.211766694485.47755533617-0.19147232180.277569017945-0.0334435001379-0.1478119403620.08301000868110.1146095369030.0863707039315-0.6605526366580.3038724402120.612955136757-0.06816653989880.04157851860770.3748601494340.06292976705070.37963082793734.749734358520.1678949479-43.491619172
202.728897576063.780430318292.258917599466.264391078933.794672168438.73333641935-0.1764513926840.5369691368910.192097227684-0.3858650143360.04790875449161.064003017330.0724422883637-0.4247966237860.2799745794420.281752025913-0.01799276941760.008113616172070.1794014102890.02419056341630.37823546075728.05220368068.34171965633-44.9776077629
219.234417251551.464718532120.2357764253093.498372818130.1232831484876.513929404020.147492717839-0.38166328688-0.122872737604-0.128987630627-0.2091043122890.05248218831070.144971247414-0.273267436743-0.06222861069450.2627389574610.0471818182344-0.03074514726820.117027679861-0.02694438614850.18091071154939.6444189294-14.7885930695-31.1822033062
226.929212833980.3147265426181.458862134761.468452624720.7541213760414.80303507442-0.2760758453940.001927758531370.448313624801-0.56565638283-0.1944801259890.157440590097-0.315247478729-0.4354107616070.03488686009630.2506371537220.0521128435634-0.01155535008720.2145019779110.01348999694340.29212500086733.1246749588-11.0611912657-37.8389338204
237.672204946311.191806812523.145051541123.25153688426-0.1353495571372.14193475830.321922373228-0.2574217445041.91580548560.06006334844950.0210665829778-0.0832100265247-1.997053040230.784524334313-0.1409141262160.785388304956-0.1239513162540.04860524654080.3546936840210.126086682030.77816795162946.4705255244-2.94336378455-37.1361253798
247.346534756622.63789924295-1.501488988522.61122752940.8161194788145.704804301260.01880686176310.509306016398-0.6031713149390.3499024367520.280546873158-0.2681226823670.6726581609480.0166174279193-0.1292164200640.3744437024860.0818019108236-0.1040353707820.270122715819-0.07186140243420.39546322778538.3747479352-21.4629769488-40.6030230546
259.52520647252.6623196577-3.204128586065.51535138692-0.581805413349.444500548190.2486984393240.34884706983-0.417729351682-0.2206007221980.0517500962367-0.5096713555350.2994796983310.678881019085-0.3941136407150.3064069316640.03050806145710.004136786968120.329049410967-0.0812914883140.26750316564744.9771255176-16.1386928639-40.949749813
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 36 )
2X-RAY DIFFRACTION2chain 'A' and (resid 37 through 58 )
3X-RAY DIFFRACTION3chain 'A' and (resid 59 through 74 )
4X-RAY DIFFRACTION4chain 'A' and (resid 75 through 120 )
5X-RAY DIFFRACTION5chain 'A' and (resid 121 through 145 )
6X-RAY DIFFRACTION6chain 'A' and (resid 146 through 167 )
7X-RAY DIFFRACTION7chain 'B' and (resid 1 through 25 )
8X-RAY DIFFRACTION8chain 'B' and (resid 26 through 36 )
9X-RAY DIFFRACTION9chain 'B' and (resid 37 through 58 )
10X-RAY DIFFRACTION10chain 'B' and (resid 59 through 74 )
11X-RAY DIFFRACTION11chain 'B' and (resid 75 through 120 )
12X-RAY DIFFRACTION12chain 'B' and (resid 121 through 137 )
13X-RAY DIFFRACTION13chain 'B' and (resid 138 through 167 )
14X-RAY DIFFRACTION14chain 'C' and (resid 6 through 18 )
15X-RAY DIFFRACTION15chain 'C' and (resid 19 through 43 )
16X-RAY DIFFRACTION16chain 'C' and (resid 44 through 56 )
17X-RAY DIFFRACTION17chain 'C' and (resid 57 through 66 )
18X-RAY DIFFRACTION18chain 'C' and (resid 67 through 76 )
19X-RAY DIFFRACTION19chain 'C' and (resid 77 through 85 )
20X-RAY DIFFRACTION20chain 'C' and (resid 86 through 93 )
21X-RAY DIFFRACTION21chain 'D' and (resid 4 through 26 )
22X-RAY DIFFRACTION22chain 'D' and (resid 27 through 42 )
23X-RAY DIFFRACTION23chain 'D' and (resid 43 through 49 )
24X-RAY DIFFRACTION24chain 'D' and (resid 50 through 85 )
25X-RAY DIFFRACTION25chain 'D' and (resid 86 through 94 )

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