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- PDB-7a0w: Structure of dimeric sodium proton antiporter NhaA, at pH 8.5, cr... -

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Basic information

Entry
Database: PDB / ID: 7a0w
TitleStructure of dimeric sodium proton antiporter NhaA, at pH 8.5, crystallized with chimeric Fab antibodies
Components
  • (chimeric antibody Fab-F6, ...) x 2
  • Na(+)/H(+) antiporter NhaA
KeywordsMEMBRANE PROTEIN / sodium proton antiporter / transporter / antibody / chimeric Fab
Function / homology
Function and homology information


sodium:proton antiporter activity / regulation of pH / plasma membrane
Similarity search - Function
Na+/H+ antiporter NhaA / Na+/H+ antiporter domain superfamily / Na+/H+ antiporter 1
Similarity search - Domain/homology
1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / CARDIOLIPIN / PHOSPHATE ION / Na(+)/H(+) antiporter NhaA
Similarity search - Component
Biological speciesSalmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
Gallus gallus (chicken)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.04 Å
AuthorsFippel, A. / Gross, N.M. / Mir, S.H. / Wirth, C. / Hunte, C.
Funding support Germany, European Union, 5items
OrganizationGrant numberCountry
German Research Foundation (DFG)BIOSS - EXC-294 Germany
German Research Foundation (DFG)CIBSS - EXC-2189 - Project ID 390939984 Germany
German Research Foundation (DFG)CRC - 1381 - Project ID 403222702 Germany
German Research Foundation (DFG)CRC - 746 Germany
European Communitys Seventh Framework ProgrammeEDICT - HEALTH-F4-2007-201924European Union
CitationJournal: To Be Published
Title: Molecular determinants for substrate uptake in electrogenic sodium/proton antiporters
Authors: Fippel, A. / Mir, S.H. / Wirth, C. / Gross, N.M. / Lentes, C.J. / Bialas, E. / Frank, F. / Ulbrich, M.H. / Andrade, S.L.A. / Kulik, A. / Hunte, C.
History
DepositionAug 11, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 23, 2022Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Na(+)/H(+) antiporter NhaA
B: Na(+)/H(+) antiporter NhaA
C: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
D: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
E: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
F: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)189,38418
Polymers184,4156
Non-polymers4,96912
Water4,306239
1
A: Na(+)/H(+) antiporter NhaA
B: Na(+)/H(+) antiporter NhaA
hetero molecules


  • defined by author
  • Evidence: gel filtration, assessed by SEC-MALLS (Lentes, C. J. et al. Molecular characterization of the Na+/H+-antiporter NhaA from Salmonella Typhimurium. PloS one 9, e101575, doi:10.1371/journal.pone.0101575 (2014))
  • 91.9 kDa, 2 polymers
  • Search similar-shape structures of this assembly by Omokage search (details)
Theoretical massNumber of molelcules
Total (without water)91,88011
Polymers87,0172
Non-polymers4,8629
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
D: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,8055
Polymers48,6992
Non-polymers1063
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
E: chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain
F: chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain


Theoretical massNumber of molelcules
Total (without water)48,6992
Polymers48,6992
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)112.788, 92.163, 139.105
Angle α, β, γ (deg.)90.000, 109.885, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Na(+)/H(+) antiporter NhaA / Sodium/proton antiporter NhaA


Mass: 43508.680 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 (bacteria)
Gene: nhaA, STM0039 / Production host: Escherichia coli (E. coli) / Variant (production host): NM554 / References: UniProt: Q8ZRZ3

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Antibody , 2 types, 4 molecules CEDF

#2: Antibody chimeric antibody Fab-F6, heavy chain,chimeric antibody Fab-F6, heavy chain


Mass: 26305.029 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: chimeric Fab with avian VH and human CH1 regions,chimeric Fab with avian VH and human CH1 regions
Source: (gene. exp.) Gallus gallus (chicken), (gene. exp.) Homo sapiens (human)
Production host: Escherichia coli (E. coli) / Variant (production host): JM83
#3: Antibody chimeric antibody Fab-F6, light chain,chimeric antibody Fab-F6, light chain


Mass: 22393.760 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: chimeric Fab with avian VL and human CL regions,chimeric Fab with avian VL and human CL regions
Source: (gene. exp.) Gallus gallus (chicken), (gene. exp.) Homo sapiens (human)
Production host: Escherichia coli (E. coli) / Variant (production host): JM83

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Non-polymers , 6 types, 251 molecules

#4: Chemical ChemComp-CDL / CARDIOLIPIN / DIPHOSPHATIDYL GLYCEROL / BIS-(1,2-DIACYL-SN-GLYCERO-3-PHOSPHO)-1',3'-SN-GLYCEROL


Mass: 1464.043 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C81H156O17P2 / Comment: phospholipid*YM
#5: Chemical
ChemComp-3PH / 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / PHOSPHATIDIC ACID


Mass: 704.998 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C39H77O8P
#6: Chemical ChemComp-PO4 / PHOSPHATE ION


Mass: 94.971 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: PO4
#7: Chemical ChemComp-PG4 / TETRAETHYLENE GLYCOL


Mass: 194.226 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H18O5 / Comment: precipitant*YM
#8: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Cl
#9: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 239 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.2 Å3/Da / Density % sol: 66.64 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 100 mM Tris pH 8.5, 100 mM MgCl2, 100 mM NaCl, 32 % PEG400

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID30B / Wavelength: 0.979 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 13, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2.04→24.86 Å / Num. obs: 94893 / % possible obs: 94.1 % / Redundancy: 13.1 % / Biso Wilson estimate: 52.71 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.087 / Net I/σ(I): 16
Reflection shellResolution: 2.04→2.34 Å / Redundancy: 11.1 % / Rmerge(I) obs: 1.68 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 4739 / CC1/2: 0.428 / % possible all: 67.6

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Processing

Software
NameVersionClassification
MxCuBEdata collection
PHENIX1.13_2998refinement
STARANISOdata scaling
XDSdata reduction
PHASERphasing
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1zcd, 2yk1

1zcd

2yk1


Resolution: 2.04→24.86 Å / SU ML: 0.2395 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 37.5384 / Stereochemistry target values: GeoStd + Monomer Library
RfactorNum. reflection% reflection
Rfree0.2347 2414 2.54 %
Rwork0.1982 92469 -
obs0.1991 94883 56.06 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 78.38 Å2
Refinement stepCycle: LAST / Resolution: 2.04→24.86 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11858 0 185 239 12282
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.003812363
X-RAY DIFFRACTIONf_angle_d0.654916829
X-RAY DIFFRACTIONf_chiral_restr0.04491966
X-RAY DIFFRACTIONf_plane_restr0.00452087
X-RAY DIFFRACTIONf_dihedral_angle_d10.45737264
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.04-2.120.408690.3049193X-RAY DIFFRACTION1.2
2.12-2.20.32250.28571018X-RAY DIFFRACTION6.21
2.2-2.30.2921590.2972277X-RAY DIFFRACTION13.85
2.3-2.420.3181060.28714073X-RAY DIFFRACTION24.82
2.42-2.580.29361910.27677132X-RAY DIFFRACTION43.35
2.58-2.770.28432930.262812156X-RAY DIFFRACTION73.84
2.77-3.050.28274650.243515745X-RAY DIFFRACTION95.87
3.05-3.490.24874180.206516499X-RAY DIFFRACTION99.99
3.49-4.40.20664100.173416618X-RAY DIFFRACTION99.98
4.4-24.860.22054380.183216758X-RAY DIFFRACTION99.8
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.998478039920.7720079808991.579689349142.96728585457-0.2220253381941.17425463364-0.1490632127190.6129827572550.0997372267759-0.1188276925120.176467775352-0.127290286501-0.1391033780580.126503778888-0.01327785725920.5085079073930.08198083524430.07388620153910.731042928555-0.006222635712720.2027014478267.49518734466.643276546432.5908589108
21.94749946999-0.3584227904520.1387010182252.53690479751.072119509993.081939783850.115695025521.39068671444-0.274536290073-0.967703401138-0.220127580499-0.6830502384330.3027173469980.557506110436-0.003512074498810.7194087304870.2080048379070.2334721662861.13478466471-0.1957239249490.64253818130726.0001408467-5.4259699638230.0968682409
33.464512259611.082152189941.480128237281.551519948011.29321063751.689333395190.005878706180040.645902890821-0.976972978205-0.3389082038150.154237724818-0.5678919447110.3267834324290.102581206474-0.07312898711520.8254271124310.1638244034590.0670799083090.962341459007-0.3169775440410.79243731924610.1619675809-11.395370325827.3710058275
41.71248965546-0.4081830167260.04293366861231.11164772768-0.1083297776931.86551320604-0.02910376108961.07618128118-0.334022912385-0.603258166623-0.0984080468866-0.280163055973-0.0189630530320.2106097087630.07798873133690.6566599088110.06033917417540.2762511461220.932550805347-0.1605992362980.4064474911414.2196846591-2.2178929477725.892781703
50.7972159959960.441287973992-0.2301544723842.562815715690.279343849861.89635939362-0.09757068886991.40321205752-0.172184350866-1.0841939599-0.309312593289-0.5600268402540.09139506985220.6271706620440.03788970426440.6429445818290.1597784005360.3504544159821.10964164254-0.01647547445130.36952385507826.78440373370.5275316302131.6090585742
63.01144343053-0.0248014504088-0.1299590955373.65946294841.587357361690.849452915977-0.05369507100061.07572490771-0.346108816643-0.167697105108-0.1471554934180.3253139120130.186482795415-0.275224053520.2406976628110.6359742994880.04465255562010.09258970524821.14776907833-0.1241159794290.272109369996-19.91485946780.49108975939523.0350755837
71.26087871029-1.02218371227-1.730621202241.69631226736-0.1574635561366.937457478630.2251353524921.74004888363-0.0330973463546-0.457702734013-0.5492978569680.114133465666-0.824376651052-0.300323785990.08400000184640.9689383150170.0152662831335-0.1994203393121.947397139970.07947930073190.511804642428-32.459033184312.15339712929.00366462165
81.73779533418-0.127188449649-0.2252344576711.389257524170.417518351521.25782123477-0.2871723850971.516315900190.537749933674-0.6963215940480.131907827420.181277916786-0.189773500868-0.3101269829330.1717011554390.926242410696-0.04687222849160.0358254498681.472647422570.1205455186090.374046475529-15.90972434311.264509608315.6105686781
91.507590995530.0344562240160.1574214629332.023291530260.3274360363182.57298601111-0.09642961604631.726045554890.47605490378-1.05355595622-0.03134618922490.279618069499-0.253878320821-0.6235472165380.05736183850461.08067577245-0.0477573803663-0.1379491606272.11327381912-0.1110187965480.0302398838701-36.37177406267.359354679738.58552249141
102.93073305179-0.073413846654-0.2967579927524.53006478322-0.738676476233.983492573270.051577398295-0.0456465404649-0.2601392448250.08546658084930.003490284245510.257325779816-0.05667058856050.0656985193485-0.03522656117970.168842348549-0.01012329766020.02525744880030.0587479463063-0.03884940068430.29492139676712.291666647-8.0941302822766.7338261601
116.04943531163-3.20492479746-4.8720178894.339837438621.418314849876.23208848844-0.857158138633-0.980786154866-0.9857406154261.403757064320.3711115046751.58538316469-0.117711468905-0.1791887527590.4640790522351.006977752330.1679273797020.3450911160410.6839327915790.08007923518710.931996663564-1.3539461932-2.6625196580893.2112977998
122.05067741819-1.25707203778-0.814644036593.20198441170.3182371239632.05952412514-0.0415119744363-0.03767057476560.5394596389530.377924206232-0.04425204314060.00993267054297-0.355834601440.2770055670180.0440081670.383245149721-0.0397217651580.008734389873370.0954731960577-0.03296768145770.39519090554412.08383457415.355274278565.4138681004
133.91151700383-1.13110660737-1.023477521635.776854409591.29542834314.81247783980.1696016193460.2511443434740.1347735795230.0674799027832-0.3043774431560.560069250003-0.420925838001-0.4712458581580.1195206145510.2931816902640.0201384562525-0.01138416855450.169706685499-0.05200815375950.452132858575.29316091211.626525343561.574243656
140.5427368802090.5015891033570.6077445018582.4109769137-0.4702199522382.58472700841-0.058954048705-0.08965987818380.2447423379010.5179127348730.07517302171540.264426045232-0.522908084583-0.175419631221-0.01980343591420.460937642660.02299721691230.06167337329360.128326892414-0.06329431746120.4156340810127.9782662043714.124693310170.7885910095
157.1798500102-4.093520951294.449491721214.25797726731-1.264819474023.608258549910.381500279956-1.58664837161-0.29864760951-0.7014770882950.3277113618920.2167442282061.06624430164-0.803055580599-0.2326475710942.263270885080.4599250782530.3419214658461.235978333460.1594912589040.574189320793-0.1844155122664.00309828939105.688117596
160.04589405111670.353399224479-0.5964466093583.07330308467-5.383067460149.46357725517-0.213282025823-0.5026101155570.00769930485571.693244435920.125796963395-0.0455287782468-1.544677427530.1557800402860.1474970942521.500533892590.1679209279550.1334467369270.815550310481-0.2525299493040.6543255502724.2553788019114.825216482495.9595339587
171.051837039510.007643605809140.0300549847954.415204770074.649647576674.888365490970.349243951262-1.109460813840.3492509882961.414891956740.428648660295-0.965415355763-1.059617046290.34343887304-0.7062991485781.75684516679-0.129242717284-0.1480681221061.01984121858-0.1339972395080.5055086900411.240124251412.8274447162101.895319787
182.433165064122.03549471481-0.9227522447432.07574168520.6974166385876.44518386681-0.303982418366-1.07706810457-0.1200854585491.482424609520.2760170764210.442807664802-0.2158545313330.5584160228540.1443481826031.3892240320.3864708109850.1480617965660.964339315954-0.1405829965450.6925531436933.402323661719.4064446834195.9483819345
192.429092898791.007982223032.324767588570.5537224096390.01918277404877.912982764050.0781141950305-0.2097697259110.02587134978271.002724586740.4910054249990.194191017555-0.1239293596971.2734912398-0.16116328652.041307574420.166029916720.1677773827091.28755956451-0.2671977211370.5695908786312.7674631611719.1356991273103.904804157
208.25574043773-1.26085096168-1.734973261394.524771642150.2339131495563.44700600068-0.1087405945050.4000545766630.335185001404-0.192374293389-0.0210108051031-0.371512736668-0.5869768769460.32180593310.1023849357990.379219338158-0.0658600815349-0.04442533032750.2273615794450.02881577770050.159794397287-44.596036270319.613807378145.7336522138
212.611984992810.480244239523-1.746532731580.9047317263630.1033280366983.70477857277-0.136633802457-0.014046402628-0.06085242625940.02931335585950.0021668059057-0.1084622425350.03924903822120.09236487425770.07591602302560.30206694011-0.00672745396638-0.0433984619160.128724348339-0.0004617626104840.227148760267-54.454487328511.413888383660.91456706
223.58997012084-1.318375723261.05035573864.66595525132-0.5855115142553.597899573940.0694837185279-0.5597226406180.2039271424321.07226269086-0.0382924962652-0.363704439365-0.0393939105127-0.091434193528-0.04132746194310.489839000813-0.048208645548-0.1227448947750.1906544713740.01145790719570.316846665457-58.688519816510.346240770878.8192607624
233.280691893361.06182449035-0.7924540646796.17485377971-1.254225563744.85269494334-0.3545511104430.333698556201-0.976780086539-0.166794562701-0.0805307430151-0.1697984807350.6964714352630.2149652185370.3731505748370.3046050047660.007338599664880.1563453478420.284291420463-0.01281260367370.52689649337-39.1972032103-3.2206584013347.3906922898
246.96099294277-1.583045555-0.1981824424219.75676647131-7.335019135618.05618258314-0.292278070433-0.516957146601-1.167316711430.665685539034-0.100949423166-0.589104066123-0.424981372060.5750341337620.2648395018520.334900048484-0.02548346860490.06672073655930.306572553978-0.003819711992340.603969266114-39.9718184425-3.7366189068654.0684923626
258.669513525561.667547461851.950655426447.773952746462.587571436271.09664896725-0.5510197351911.63928878278-0.0537757416542-0.9727961593920.4496435675270.791039581064-0.2179292362710.045081476521-0.0429419183920.378809580468-0.01363697751460.05577556215470.403612813832-0.1408774186830.337804918736-48.06649050544.3811709957740.0224014894
260.1561284500660.46883485689-0.6207751303491.39626992949-1.84589646412.41899740747-0.1631129129660.002195455797920.173880758659-0.257308472016-0.0894910918601-0.8677522500840.4254469039970.3404667346330.3314615927910.4828146525850.1723660802330.1176645117050.106645960390.2314146908690.825769160002-47.299757984-11.316111767265.4641140811
274.28469254115-5.655673998041.016247659197.48403019259-0.7642816242985.86140916166-0.417348472272-0.2017252609080.3743910644851.466383365730.05870902093920.626708807537-0.487036334181-0.3993803368420.3394402360580.399687631634-0.04273391166740.1060731194820.250703853753-0.03384453552270.603180428979-71.04211118793.987226915578.4757164509
288.72089159047-6.88608602189-0.02200771696598.369014953970.4207281935751.47857752244-0.008298586579920.234252559345-0.118437291318-0.107047985957-0.0168650375634-0.0710528964479-0.01720952807-0.07174602054660.03293558313010.379335814383-0.04157812389350.01643348136060.2192224431070.07241649044470.260131680594-62.9121267652-5.4340910216869.5523765227
294.121431221885.73824587021.981549427388.865433245212.229431824192.906542037410.172552352060.209255943499-0.380451249794-0.432660362613-0.2858658220560.5171990688710.599893560621-0.5179222557910.1409456134050.546567107744-0.00129839145125-0.09626263338650.318589358267-0.05186610124530.336851856605-70.7675551681-3.1148644798665.3726128867
305.180906450444.673702657415.031130398465.205124522815.773914339746.432409170260.2897603116330.8276621109411.480602959381.15377868583-0.0942128067503-2.67139513869-0.1961740196872.26792343672-0.2578628495670.459725952547-0.0392136621811-0.1421641024790.629141852430.03124198855720.966295580616-47.4669866748-3.2121709049171.0163806465
315.94642578551-3.687154773780.07372493105026.854924294990.7872101874143.274542806060.3575889387290.144415708634-0.18840538238-0.227162924216-0.563250582110.941744597520.101396867653-0.4194112545230.2089064228010.225872261098-0.0273029245746-0.07309309413480.204156878261-0.03544474375210.343627165993-73.9124758018-2.2749763337871.4887602198
323.22368763452-1.16754195272.422244524684.58323404582-1.634682953241.95559803794-0.260112602405-0.612881271121-1.374246488890.6186864648630.3587180303220.7103188055040.0276849397335-0.01305429267480.02061187837010.443001274718-0.1077035104090.03807291930360.2341381191740.05785475731140.467452395866-69.0716511179-11.304978013475.4701714659
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 12 through 84 )
2X-RAY DIFFRACTION2chain 'A' and (resid 85 through 149 )
3X-RAY DIFFRACTION3chain 'A' and (resid 150 through 199 )
4X-RAY DIFFRACTION4chain 'A' and (resid 200 through 313 )
5X-RAY DIFFRACTION5chain 'A' and (resid 314 through 379 )
6X-RAY DIFFRACTION6chain 'B' and (resid 11 through 84 )
7X-RAY DIFFRACTION7chain 'B' and (resid 85 through 149 )
8X-RAY DIFFRACTION8chain 'B' and (resid 150 through 286 )
9X-RAY DIFFRACTION9chain 'B' and (resid 287 through 383 )
10X-RAY DIFFRACTION10chain 'C' and (resid 1 through 112 )
11X-RAY DIFFRACTION11chain 'C' and (resid 113 through 223 )
12X-RAY DIFFRACTION12chain 'D' and (resid 1 through 32 )
13X-RAY DIFFRACTION13chain 'D' and (resid 33 through 70 )
14X-RAY DIFFRACTION14chain 'D' and (resid 71 through 109 )
15X-RAY DIFFRACTION15chain 'D' and (resid 110 through 124 )
16X-RAY DIFFRACTION16chain 'D' and (resid 125 through 146 )
17X-RAY DIFFRACTION17chain 'D' and (resid 147 through 159 )
18X-RAY DIFFRACTION18chain 'D' and (resid 160 through 184 )
19X-RAY DIFFRACTION19chain 'D' and (resid 185 through 207 )
20X-RAY DIFFRACTION20chain 'E' and (resid 1 through 83 )
21X-RAY DIFFRACTION21chain 'E' and (resid 84 through 154 )
22X-RAY DIFFRACTION22chain 'E' and (resid 155 through 223 )
23X-RAY DIFFRACTION23chain 'F' and (resid 1 through 70 )
24X-RAY DIFFRACTION24chain 'F' and (resid 71 through 86 )
25X-RAY DIFFRACTION25chain 'F' and (resid 87 through 98 )
26X-RAY DIFFRACTION26chain 'F' and (resid 99 through 109 )
27X-RAY DIFFRACTION27chain 'F' and (resid 110 through 124 )
28X-RAY DIFFRACTION28chain 'F' and (resid 125 through 146 )
29X-RAY DIFFRACTION29chain 'F' and (resid 147 through 159 )
30X-RAY DIFFRACTION30chain 'F' and (resid 160 through 170 )
31X-RAY DIFFRACTION31chain 'F' and (resid 171 through 194 )
32X-RAY DIFFRACTION32chain 'F' and (resid 195 through 209 )

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