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Yorodumi- PDB-6xpr: Human antibody D2 H1-1/H3-1 H3 in complex with the influenza hema... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6xpr | ||||||||||||
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Title | Human antibody D2 H1-1/H3-1 H3 in complex with the influenza hemagglutinin head domain of A/Texas/50/2012(H3N2) | ||||||||||||
Components |
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Keywords | VIRAL PROTEIN/IMMUNE SYSTEM / human antibody antigen complex / hemagglutinin / influenza / VIRAL PROTEIN-IMMUNE SYSTEM complex | ||||||||||||
Function / homology | Function and homology information viral budding from plasma membrane / clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / virion membrane Similarity search - Function | ||||||||||||
Biological species | Influenza A virus Homo sapiens (human) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.092 Å | ||||||||||||
Authors | McCarthy, K.R. / Harrison, S.C. / Lee, J. | ||||||||||||
Funding support | United States, 3items
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Citation | Journal: Mbio / Year: 2021 Title: A Prevalent Focused Human Antibody Response to the Influenza Virus Hemagglutinin Head Interface. Authors: McCarthy, K.R. / Lee, J. / Watanabe, A. / Kuraoka, M. / Robinson-McCarthy, L.R. / Georgiou, G. / Kelsoe, G. / Harrison, S.C. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6xpr.cif.gz | 288.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6xpr.ent.gz | 231.5 KB | Display | PDB format |
PDBx/mmJSON format | 6xpr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xp/6xpr ftp://data.pdbj.org/pub/pdb/validation_reports/xp/6xpr | HTTPS FTP |
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-Related structure data
Related structure data | 6xpoC 6xpqC 6xpxC 6xpyC 6xpzC 6xq0C 6xq2C 6xq4C 4y5vS 5w08S 6e4xS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 1 types, 2 molecules AD
#1: Protein | Mass: 32618.846 Da / Num. of mol.: 2 / Fragment: head domain (UNP residues 53-335) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus (A/Texas/50/2012(H3N2)) Strain: A/Texas/50/2012(H3N2) / Gene: HA, L998_47834gpHA / Cell line (production host): BTI-Tn-5B1-4 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: R4L1D1 |
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-Antibody , 2 types, 4 molecules BECF
#2: Antibody | Mass: 25692.869 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): 293F / Production host: Homo sapiens (human) #3: Antibody | Mass: 23252.756 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): 293F / Production host: Homo sapiens (human) |
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-Sugars , 3 types, 6 molecules
#4: Polysaccharide | Source method: isolated from a genetically manipulated source #5: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #6: Sugar | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.9 Å3/Da / Density % sol: 74.91 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 0.1 M lithium sulfate, 30% v/v PEG400, 0.1 M trisodium citrate, pH 5.6 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9791 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 23, 2018 |
Radiation | Monochromator: Cryo-Cooled double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 4.092→49.12 Å / Num. obs: 25192 / % possible obs: 98.41 % / Redundancy: 3.4 % / Biso Wilson estimate: 97.78 Å2 / CC1/2: 0.965 / CC star: 0.991 / Rmerge(I) obs: 0.3059 / Rpim(I) all: 0.1897 / Rrim(I) all: 0.3614 / Net I/σ(I): 4.53 |
Reflection shell | Resolution: 4.092→4.238 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.855 / Mean I/σ(I) obs: 1.6 / Num. unique obs: 8466 / CC1/2: 0.776 / CC star: 0.935 / Rpim(I) all: 0.5332 / Rrim(I) all: 1.012 / % possible all: 98.07 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entries 5W08, 4Y5V, & 6E4X Resolution: 4.092→49.117 Å / SU ML: 0.59 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 32.58 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 255.6 Å2 / Biso mean: 131.0644 Å2 / Biso min: 26.12 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 4.092→49.117 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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