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- PDB-6x90: Structure of the guanine nucleotide exchange factor Sec12 bound t... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6x90 | ||||||
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Title | Structure of the guanine nucleotide exchange factor Sec12 bound to the small GTPase Sar1 | ||||||
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![]() | PROTEIN TRANSPORT / Endoplasmic reticulum / GTPase / exchange factor / membrane trafficking | ||||||
Function / homology | ![]() Antigen Presentation: Folding, assembly and peptide loading of class I MHC / Cargo concentration in the ER / regulation of COPII vesicle coating / positive regulation of ER to Golgi vesicle-mediated transport / mitochondria-associated endoplasmic reticulum membrane contact site / COPII-mediated vesicle transport / nuclear envelope organization / vesicle organization / COPII vesicle coat / positive regulation of protein exit from endoplasmic reticulum ...Antigen Presentation: Folding, assembly and peptide loading of class I MHC / Cargo concentration in the ER / regulation of COPII vesicle coating / positive regulation of ER to Golgi vesicle-mediated transport / mitochondria-associated endoplasmic reticulum membrane contact site / COPII-mediated vesicle transport / nuclear envelope organization / vesicle organization / COPII vesicle coat / positive regulation of protein exit from endoplasmic reticulum / membrane organization / mitochondrial fission / mitochondrial membrane organization / endoplasmic reticulum exit site / protein secretion / endoplasmic reticulum to Golgi vesicle-mediated transport / GTPase activator activity / guanyl-nucleotide exchange factor activity / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / intracellular protein transport / Golgi membrane / GTPase activity / endoplasmic reticulum membrane / GTP binding / Golgi apparatus / endoplasmic reticulum / mitochondrion Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Joiner, A.M.N. / Fromme, J.C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for the initiation of COPII vesicle biogenesis. Authors: Joiner, A.M.N. / Fromme, J.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 233 KB | Display | ![]() |
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PDB format | ![]() | 165 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.3 KB | Display | ![]() |
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Full document | ![]() | 432.6 KB | Display | |
Data in XML | ![]() | 18.7 KB | Display | |
Data in CIF | ![]() | 25.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39369.695 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: SEC12, SED2, YNR026C, N3244 / Production host: ![]() ![]() |
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#2: Protein | Mass: 19596.312 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: SAR1, YPL218W / Production host: ![]() ![]() References: UniProt: P20606, Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement |
#3: Chemical | ChemComp-K / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.31 Å3/Da / Density % sol: 46.66 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / Details: 5mg/mL protein, 100mM MES pH6.5, 30% PEG 5000 MME |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 25, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.26→57.71 Å / Num. obs: 20215 / % possible obs: 95.3 % / Redundancy: 6.5 % / Biso Wilson estimate: 49.22 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.039 / Rpim(I) all: 0.017 / Rrim(I) all: 0.043 / Net I/σ(I): 20.1 |
Reflection shell | Resolution: 2.26→2.33 Å / Redundancy: 6.4 % / Rmerge(I) obs: 0.741 / Mean I/σ(I) obs: 2.4 / Num. unique obs: 1012 / CC1/2: 0.854 / Rpim(I) all: 0.313 / Rrim(I) all: 0.806 / % possible all: 98.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4H5I, 1F6B Resolution: 2.26→57.71 Å / SU ML: 0.3257 / Cross valid method: FREE R-VALUE / Phase error: 32.2202 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 66.16 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.26→57.71 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group | Refine-ID: X-RAY DIFFRACTION
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