- PDB-1f6b: CRYSTAL STRUCTURE OF SAR1-GDP COMPLEX -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 1f6b
Title
CRYSTAL STRUCTURE OF SAR1-GDP COMPLEX
Components
SAR1
Keywords
PROTEIN TRANSPORT / GTPASES / N-TERMINAL HELIX / MG-CONTAINING COMPLEX
Function / homology
Function and homology information
lipid export from cell / regulation of lipid transport / regulation of COPII vesicle coating / lipoprotein transport / COPII-coated vesicle cargo loading / COPII vesicle coat / positive regulation of protein exit from endoplasmic reticulum / COPII vesicle coating / lipid homeostasis / Golgi cisterna membrane ...lipid export from cell / regulation of lipid transport / regulation of COPII vesicle coating / lipoprotein transport / COPII-coated vesicle cargo loading / COPII vesicle coat / positive regulation of protein exit from endoplasmic reticulum / COPII vesicle coating / lipid homeostasis / Golgi cisterna membrane / endoplasmic reticulum exit site / endoplasmic reticulum to Golgi vesicle-mediated transport / small monomeric GTPase / G protein activity / intracellular protein transport / lysosomal membrane / endoplasmic reticulum membrane / GTP binding / metal ion binding / cytosol Similarity search - Function
small GTPase SAR1 family profile. / Small GTPase superfamily, SAR1-type / Sar1p-like members of the Ras-family of small GTPases / Small GTPase superfamily, ARF/SAR type / ADP-ribosylation factor family / ARF-like small GTPases; ARF, ADP-ribosylation factor / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase ...small GTPase SAR1 family profile. / Small GTPase superfamily, SAR1-type / Sar1p-like members of the Ras-family of small GTPases / Small GTPase superfamily, ARF/SAR type / ADP-ribosylation factor family / ARF-like small GTPases; ARF, ADP-ribosylation factor / Small GTP-binding protein domain / P-loop containing nucleotide triphosphate hydrolases / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology
The missing residues in remark 465 are either in N-terminal or are part of flexible switch regions ... The missing residues in remark 465 are either in N-terminal or are part of flexible switch regions (switch I: 48-59; switch II: 77-94
Mass: 18.015 Da / Num. of mol.: 297 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.49 Å3/Da / Density % sol: 50.57 %
Crystal grow
Temperature: 295.5 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: 30% PEG 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate at pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K
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Data collection
Diffraction
ID
Mean temperature (K)
Crystal-ID
1
173
1
2
173
1
3
1
4
1
Diffraction source
Source
Site
Beamline
ID
Wavelength
SYNCHROTRON
SSRL
BL7-1
1
1.08
SYNCHROTRON
SSRL
BL7-1
2
1.08
SYNCHROTRON
SSRL
BL9-2
3
0.9796
SYNCHROTRON
SSRL
BL9-2
4
0.9795
Detector
ID
Detector
Date
1
IMAGE PLATE
Feb 13, 1999
2
IMAGE PLATE
Jun 1, 1999
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
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