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- PDB-1rrg: NON-MYRISTOYLATED RAT ADP-RIBOSYLATION FACTOR-1 COMPLEXED WITH GD... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1rrg | ||||||
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Title | NON-MYRISTOYLATED RAT ADP-RIBOSYLATION FACTOR-1 COMPLEXED WITH GDP, DIMERIC CRYSTAL FORM | ||||||
![]() | RAT ADP-RIBOSYLATION FACTOR-1 | ||||||
![]() | TRANSPORT PROTEIN / GDP-BINDING / MEMBRANE TRAFFICKING / HOMODIMER | ||||||
Function / homology | ![]() Glycosphingolipid transport / Synthesis of PIPs at the Golgi membrane / synaptic vesicle budding / lysosomal membrane organization / positive regulation of late endosome to lysosome transport / regulation of phospholipid metabolic process / Synthesis of PIPs at the plasma membrane / Golgi to transport vesicle transport / phospholipase D activator activity / Intra-Golgi traffic ...Glycosphingolipid transport / Synthesis of PIPs at the Golgi membrane / synaptic vesicle budding / lysosomal membrane organization / positive regulation of late endosome to lysosome transport / regulation of phospholipid metabolic process / Synthesis of PIPs at the plasma membrane / Golgi to transport vesicle transport / phospholipase D activator activity / Intra-Golgi traffic / COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / trans-Golgi Network Vesicle Budding / mitotic cleavage furrow ingression / positive regulation of ER to Golgi vesicle-mediated transport / Lysosome Vesicle Biogenesis / regulation of receptor internalization / very-low-density lipoprotein particle assembly / Golgi Associated Vesicle Biogenesis / regulation of Arp2/3 complex-mediated actin nucleation / MHC class II antigen presentation / postsynaptic actin cytoskeleton organization / positive regulation of calcium ion-dependent exocytosis / dendritic spine organization / long-term synaptic depression / positive regulation of sodium ion transmembrane transport / positive regulation of dendritic spine development / cell leading edge / positive regulation of endocytosis / intracellular copper ion homeostasis / endomembrane system / vesicle-mediated transport / sarcomere / small monomeric GTPase / positive regulation of protein secretion / actin filament organization / intracellular protein transport / GDP binding / late endosome / postsynaptic density / neuron projection / protein domain specific binding / Golgi membrane / GTPase activity / glutamatergic synapse / GTP binding / Golgi apparatus / magnesium ion binding / protein-containing complex / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Greasley, S.E. / Jhoti, H. / Bax, B. | ||||||
![]() | ![]() Title: The structure of rat ADP-ribosylation factor-1 (ARF-1) complexed to GDP determined from two different crystal forms. Authors: Greasley, S.E. / Jhoti, H. / Teahan, C. / Solari, R. / Fensome, A. / Thomas, G.M. / Cockcroft, S. / Bax, B. #1: ![]() Title: Crystallization and Preliminary X-Ray Diffraction Studies on Adp-Ribosylation Factor 1 Authors: Greasley, S. / Jhoti, H. / Fensome, A.C. / Cockcroft, S. / Thomas, G.M. / Bax, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 89.2 KB | Display | ![]() |
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PDB format | ![]() | 67.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 18.3 KB | Display | |
Data in CIF | ![]() | 25.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 20721.742 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Compound details | SECONDARY STRUCTURAL | Nonpolymer details | MAGNESIUM MAY NOT BE FULLY OCCUPIED OR MAY BE A WATER MOLECULE. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 45 % | |||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging dropDetails: protein solution is mixed in a 1:1 ratio with well solution | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 30, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→30 Å / Num. obs: 13030 / % possible obs: 90 % / Observed criterion σ(I): 0 / Redundancy: 3.01 % / Rmerge(I) obs: 0.081 |
Reflection | *PLUS Num. measured all: 40351 |
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Processing
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Refinement | Resolution: 2.4→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 42.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |