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Open data
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Basic information
Entry | Database: PDB / ID: 6wkx | ||||||
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Title | Cryo-EM of Form 1 related peptide filament, 15-10-3 | ||||||
![]() | peptide 15-10-3 | ||||||
![]() | PROTEIN FIBRIL / ![]() ![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() | ||||||
![]() | Wang, F. / Gnewou, O.M. / Egelman, E.H. / Conticello, V.P. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM of Form 1 related peptide filament, 15-10-3 Authors: Wang, F. / Gnewou, O.M. / Egelman, E.H. / Conticello, V.P. | ||||||
Validation Report | ![]() ![]() ![]() | ||||||
History |
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Structure visualization
Movie |
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Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmcif format | ![]() ![]() ![]() |
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PDBML Plus | ![]() |
Others | ![]() |
-Related structure data
Related structure data | ![]() 21812CM C: citing same article ( M: map data used to model this data |
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Similar-shape strucutres |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Symmetry | Helical symmetry: (Circular symmetry: 5 / Dyad axis: no / N subunits divisor: 1 / Num. of operations: 105 / Rise per n subunits: 9.23 Å / Rotation per n subunits: 9.31 °) |
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Components
#1: Protein/peptide | Mass: 1747.004 Da / Num. of mol.: 105 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
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Sample preparation
Component | Name: self-assembly peptide filament, 15-10-3 / Type: COMPLEX / Details: synthetic peptide / Entity ID: #1 / Source: NATURAL |
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Source (natural) | Organism: synthetic construct (others) |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() |
Specimen support | Details: unspecified |
Vitrification![]() | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Microscopy | Model: FEI TALOS ARCTICA |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Image recording | Electron dose: 54 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
Software | Name: PHENIX / Version: 1.15.2_3472: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
Helical symmerty | Angular rotation/subunit: 9.31 ° / Axial rise/subunit: 9.23 Å / Axial symmetry: C5 | ||||||||||||||||||||||||
3D reconstruction![]() | Resolution: 4.2 Å / Resolution method: OTHER / Num. of particles: 14700 / Details: Model:Map FSC 0.38 cut off and d99 / Symmetry type: HELICAL | ||||||||||||||||||||||||
Refine LS restraints |
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