+
Open data
-
Basic information
Entry | Database: PDB / ID: 6wl8 | ||||||
---|---|---|---|---|---|---|---|
Title | Cryo-EM of Form 2 peptide filament | ||||||
![]() | Form 2 peptide | ||||||
![]() | PROTEIN FIBRIL / ![]() ![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() | ||||||
![]() | Wang, F. / Gnewou, O.M. / Xu, C. / Su, Z. / Egelman, E.H. / Conticello, V.P. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Cryo-EM of Form 2 peptide filament Authors: Wang, F. / Gnewou, O.M. / Xu, C. / Su, Z. / Egelman, E.H. / Conticello, V.P. | ||||||
Validation Report | ![]() ![]() ![]() | ||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | Molecule: ![]() ![]() |
-
Downloads & links
-
Download
PDBx/mmcif format | ![]() ![]() ![]() |
---|---|
PDBML Plus | ![]() |
Others | ![]() |
-Related structure data
Related structure data | ![]() 21817CM C: citing same article ( M: map data used to model this data |
---|---|
Similar-shape strucutres |
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
Symmetry | Helical symmetry: (Circular symmetry: 1 / Dyad axis: no / N subunits divisor: 1 / Num. of operations: 106 / Rise per n subunits: 1.93 Å / Rotation per n subunits: 124.36 °) |
-
Components
#1: Protein/peptide | Mass: 3197.741 Da / Num. of mol.: 106 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|---|
EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
-
Sample preparation
Component | Name: self-assembly Form 2 peptide filament / Type: COMPLEX / Details: synthetic peptide / Entity ID: #1 / Source: NATURAL |
---|---|
Source (natural) | Organism: synthetic construct (others) |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() |
Vitrification![]() | Cryogen name: ETHANE |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Image recording | Electron dose: 51 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-
Processing
Software | Name: PHENIX / Version: 1.14_3260: / Classification: refinement |
---|---|
CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
Helical symmerty | Angular rotation/subunit: 124.36 ° / Axial rise/subunit: 1.93 Å / Axial symmetry: C1 |
3D reconstruction![]() | Resolution: 4.1 Å / Resolution method: OTHER / Num. of particles: 408751 / Details: Model:Map FSC 0.38 cut off and d99 / Symmetry type: HELICAL |