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- PDB-6vze: C-terminal domain of mouse surfactant protein B crystallized at low pH -

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Basic information

Entry
Database: PDB / ID: 6vze
TitleC-terminal domain of mouse surfactant protein B crystallized at low pH
ComponentsPulmonary surfactant-associated protein B
KeywordsSURFACTANT PROTEIN / Saposin-like
Function / homology
Function and homology information


Surfactant metabolism / sphingolipid metabolic process / respiratory gaseous exchange by respiratory system / : / lysosome / extracellular region / membrane / cytoplasm
Similarity search - Function
Saposin A-type domain / Saposin / : / Saposin A-type domain / Saposin A-type domain profile. / Saposin/surfactant protein-B A-type DOMAIN / Saposin-like type B, region 1 / Saposin-like type B, region 1 / Saposin B type, region 2 / Saposin-like type B, region 2 ...Saposin A-type domain / Saposin / : / Saposin A-type domain / Saposin A-type domain profile. / Saposin/surfactant protein-B A-type DOMAIN / Saposin-like type B, region 1 / Saposin-like type B, region 1 / Saposin B type, region 2 / Saposin-like type B, region 2 / Saposin (B) Domains / Saposin B type domain / Saposin-like / Saposin B type domain profile.
Similarity search - Domain/homology
Pulmonary surfactant-associated protein B
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å
AuthorsRapoport, T.A. / Bodnar, N.O.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) United States
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: Mol.Cell / Year: 2021
Title: Mechanism of Lamellar Body Formation by Lung Surfactant Protein B.
Authors: Sever, N. / Milicic, G. / Bodnar, N.O. / Wu, X. / Rapoport, T.A.
History
DepositionFeb 28, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 25, 2020Provider: repository / Type: Initial release
Revision 1.1Dec 2, 2020Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.title / _citation.year
Revision 1.2Dec 9, 2020Group: Database references / Category: citation / Item: _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.3Jan 20, 2021Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.year
Revision 1.4Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id
Revision 1.5Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
B: Pulmonary surfactant-associated protein B
E: Pulmonary surfactant-associated protein B
H: Pulmonary surfactant-associated protein B
D: Pulmonary surfactant-associated protein B
F: Pulmonary surfactant-associated protein B
G: Pulmonary surfactant-associated protein B
C: Pulmonary surfactant-associated protein B
A: Pulmonary surfactant-associated protein B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)70,31211
Polymers70,0248
Non-polymers2883
Water3,009167
1
B: Pulmonary surfactant-associated protein B
D: Pulmonary surfactant-associated protein B
C: Pulmonary surfactant-associated protein B
A: Pulmonary surfactant-associated protein B
hetero molecules


  • defined by author&software
  • Evidence: The total buried surface area between the two dimers that form the apparent tetramer is approximately 1000 square angstroms, suggesting that the tetramer could represent a physiologic ...Evidence: The total buried surface area between the two dimers that form the apparent tetramer is approximately 1000 square angstroms, suggesting that the tetramer could represent a physiologic complex. In addition, the tetramer crystallizes at low pH and the protein is found in vivo in the lysosome-like lamellar body organelle, which also has a low pH.
  • 35.2 kDa, 4 polymers
  • Search similar-shape structures of this assembly by Omokage search (details)
Theoretical massNumber of molelcules
Total (without water)35,2046
Polymers35,0124
Non-polymers1922
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7940 Å2
ΔGint-85 kcal/mol
Surface area15680 Å2
MethodPISA
2
E: Pulmonary surfactant-associated protein B
H: Pulmonary surfactant-associated protein B
F: Pulmonary surfactant-associated protein B
G: Pulmonary surfactant-associated protein B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)35,1085
Polymers35,0124
Non-polymers961
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8150 Å2
ΔGint-76 kcal/mol
Surface area15160 Å2
MethodPISA
Unit cell
Length a, b, c (Å)52.193, 79.008, 71.917
Angle α, β, γ (deg.)90.000, 106.428, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
51
61
71
81

NCS domain segments:

Ens-ID: 1 / Beg auth comp-ID: THR / Beg label comp-ID: THR / End auth comp-ID: GLU / End label comp-ID: GLU / Auth seq-ID: 3 - 73 / Label seq-ID: 3 - 73

Dom-IDComponent-IDSelection detailsAuth asym-IDLabel asym-ID
11(chain 'A' and resid 3 through 73)AH
22(chain 'B' and resid 3 through 73)BA
33(chain 'C' and resid 3 through 73)CG
44(chain 'D' and resid 3 through 73)DD
55(chain 'E' and resid 3 through 73)EB
66(chain 'F' and resid 3 through 73)FE
77(chain 'G' and resid 3 through 73)GF
88(chain 'H' and resid 3 through 73)HC

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Components

#1: Protein
Pulmonary surfactant-associated protein B / SP-B / Pulmonary surfactant-associated proteolipid SPL(Phe)


Mass: 8752.949 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Sftpb, Sftp3 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta-gami 2 / References: UniProt: P50405
#2: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: SO4
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 167 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.03 Å3/Da / Density % sol: 39.44 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 4.5
Details: 170 mM ammonium sulfate, 21-23% PEG 4000, 15% glycerol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.979 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Nov 16, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 1.9→68.98 Å / Num. obs: 43545 / % possible obs: 97.42 % / Redundancy: 3.5 % / Biso Wilson estimate: 36.19 Å2 / CC1/2: 0.991 / Rmerge(I) obs: 0.1057 / Net I/σ(I): 9.54
Reflection shellResolution: 1.9→1.968 Å / Redundancy: 3.5 % / Rmerge(I) obs: 1.102 / Mean I/σ(I) obs: 1.09 / Num. unique obs: 4071 / CC1/2: 0.211 / % possible all: 93.14

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
XDSdata scaling
Cootmodel building
PHENIXphasing
XDSdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6VZ0, residues A26-V45

6vz0


Resolution: 1.9→68.98 Å / SU ML: 0.2798 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.0944
RfactorNum. reflection% reflection
Rfree0.2398 2129 4.95 %
Rwork0.2102 --
obs0.2117 43047 97.23 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 49.21 Å2
Refinement stepCycle: LAST / Resolution: 1.9→68.98 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4680 0 15 167 4862
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00414799
X-RAY DIFFRACTIONf_angle_d0.73996502
X-RAY DIFFRACTIONf_chiral_restr0.0406698
X-RAY DIFFRACTIONf_plane_restr0.0045860
X-RAY DIFFRACTIONf_dihedral_angle_d19.01381756
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.9-1.940.38321220.37742477X-RAY DIFFRACTION88.22
1.94-1.990.3531320.31852730X-RAY DIFFRACTION97.88
1.99-2.050.33131350.2952727X-RAY DIFFRACTION97.98
2.05-2.110.31511320.27852693X-RAY DIFFRACTION95.83
2.11-2.170.28511400.26722727X-RAY DIFFRACTION97.32
2.17-2.250.27341220.25492751X-RAY DIFFRACTION97.56
2.25-2.340.30741340.22492735X-RAY DIFFRACTION98.19
2.34-2.450.22961500.21932763X-RAY DIFFRACTION98.78
2.45-2.580.27331480.22012705X-RAY DIFFRACTION97.24
2.58-2.740.28051640.2092778X-RAY DIFFRACTION99.06
2.74-2.950.2541610.21162733X-RAY DIFFRACTION98.77
2.95-3.250.27241590.20632778X-RAY DIFFRACTION98.89
3.25-3.720.18981220.18022777X-RAY DIFFRACTION97.77
3.72-4.680.18661540.1632756X-RAY DIFFRACTION97.91
4.68-68.980.22851540.21722788X-RAY DIFFRACTION97.1
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.977235062783-0.0175095882827-0.232304029380.1403493123320.1747529625021.57457924153-0.08654302479890.3725489147510.0468611134018-0.1375172191890.237637515292-0.0322254892536-0.0350791446407-0.33782621095-0.0001785601518750.275550790169-0.00265560413676-0.03076404146820.3618571725230.05713279434820.32423538578-7.7066745333871.0102681885-11.6485706527
20.2615461615240.0955989695832-0.1549113243640.376254511161-0.1667122691260.18636252068-0.1670194500580.276725586017-0.242093885075-0.4873910240050.384897278653-0.802285066868-0.01439993445990.2052123520956.88806192845E-70.347466687274-0.02661467072940.04131994106630.316332937068-0.01932123729160.31712208881514.939931435580.7167635149-13.9318700218
30.153491935347-0.002190129558010.0513134913440.1209579958260.1299810608990.1199530625030.249202019004-0.273167058328-0.832181490911-0.168076435655-0.187553315492-0.2035463730080.0526138965018-0.09021781530393.58964914617E-60.338851327218-0.00115946005361-5.45874150991E-50.390042191250.03292490315610.4305491571064.0108455600866.4344654664-12.7753933822
40.264337600419-0.10860273253-0.2882956064640.1184614300270.008816235048620.4596814275320.01910849702490.279929186346-1.38154280663-0.333429117287-0.2107465282620.5515388728440.530545309571-0.687553615633-0.007234417732110.387840787793-0.0320073487244-0.1083029845040.4416214867420.01988919459850.523389792992-12.640621322359.0256010295-3.56416035943
51.3117117341-0.2131927213710.9028138620651.072996431870.5084497248751.159827692730.1074115974130.296626613448-0.17164549626-0.037943141893-0.02938986214220.2430230460520.06466852296050.2085334008630.01261246281820.3062892394410.03575325643360.003320058510030.363844919727-0.04615835147510.29096274049110.801707235151.965751993222.2208831353
60.9341241201290.1557362148630.6468113242781.13132487853-0.3541165676420.68762867131-0.742534261964-0.595083669267-0.9011742433590.741705735640.91369320231.566738562370.69715027897-1.12603061496-0.08674505475530.565421880948-0.01303810427840.1443176249850.450178353660.1065388477750.538070021424-5.0139350100843.859013880937.4730290076
70.277811434446-0.0804935973020.2027073516650.0765166396754-0.1721651442680.3029632474540.370534491363-0.6033976217150.541195307021-0.114373641941-0.171077778370.104407616146-0.3572799012430.2643997318410.0004829510851190.3852219506960.01935417370880.06042163358450.329419611604-0.05913763107930.3370373320023.1944147956857.706187747229.3914023579
80.08558656082820.0847267495592-0.03688943139270.274115842328-0.1641289150350.3274750634550.04493335628210.4837041144520.877086174645-0.04337587601910.045381056158-0.0932141227205-0.887313170598-0.36064620273-0.0002744828442770.4883990305740.02861965503050.09088040392960.41610159767-0.008854053041280.40616460331119.242277978164.422851599323.1833785192
92.96574991649-0.1523217943412.894506677542.23323435554-0.05749100581792.853436613670.4975401614220.7754530359240.296543946997-1.17515326795-0.03114603189-1.02770373645-0.1753701634650.8133798831610.335793576880.475502001219-0.04737921299670.1738216320640.3804810355120.03830578796750.46321282636927.126432105467.656479358120.9483106452
100.4012387146030.454762764732-0.4267929793571.07530348489-0.03055639943590.597341804748-0.310993865333-0.647157139429-0.097699726763-0.02888184879420.349699920820.06020457033740.06917677689270.238530174651-1.50473886202E-60.314957342170.0338090648327-0.03155120911790.351956874326-0.006040655500320.29290780403524.331519222965.398638662943.2110798933
110.6204369320480.01797873601520.2263182240950.3602352225850.237915087690.237069742758-0.3696121918420.7812150509710.402218772624-0.3746210812730.01164978629020.173541680446-0.2579823661230.043247569642-0.03730988280240.439865758204-0.0071782909922-0.08677612699120.31742848051-0.0303499946380.4134152258812.2459526516283.644529347341.9569199047
120.8821067731270.5456285002720.1332741655440.902307858446-0.1845783816330.2099984550180.1915864808670.09081680734290.1730050696460.844349256449-0.4248858421170.9627597869210.344487351484-0.407901652291-0.09969728201870.588344197871-0.1129494531920.1616928903230.406717215039-0.1174830244240.4492018318161.2576117974671.474608062343.8366805692
131.537770014770.5128559374950.4425700206050.191885441810.1633751408910.1440984521520.00856959962237-0.267812633685-1.093918572010.2647882673710.09550648614510.1795892573020.523462307157-0.7062457891080.04386340512950.372704717887-0.0150826836616-0.02723788053770.4384831175880.07672296235960.40312659007924.542153312256.702180992937.2811607069
140.6594729987770.309789880443-0.9367303047270.368072411423-0.5608031097951.317219098420.3622764045410.1866620146080.08827767068980.0309763383035-0.03665685243890.0122889792935-0.137148666679-0.06926884228360.005159622221610.286863292535-0.01280963812910.0113149571730.2784909179320.009933480464080.312995361315-2.1786975697676.1545659832-8.25329733344
150.1997427533890.0955990482788-0.02058574830460.146431698805-0.01724965790760.1728369701350.0926760008966-0.3431866082850.3292491408710.0962225856972-0.2337290949010.803072726386-0.155907459389-0.9738788928110.03220123842240.328489331221-0.0362423061663-0.0302259704060.5824811411230.08075048398550.597772971172-25.894298729670.1067736656-8.07253082608
160.1583833235760.1331070389030.02497020543360.08545797374450.0398569313610.3013648721690.0253958604969-0.145608954499-0.16718699042-0.5718214254040.072750230823-0.390745241837-0.01325499367830.1936363394551.41670359659E-50.363442475315-0.00741708665849-0.008902674606730.373606384190.04924657439010.312010120118-10.147947988372.21516646150.159077681783
170.2071779544550.1865588615560.0006494359285190.4391906495570.1933600194840.08235438397020.600636423825-0.6919080300570.3770568637430.172856132341-0.322625649079-0.731214868397-0.4535022703770.426060800243.31895996799E-60.338755819664-0.0621070293543-0.04252629898480.4287617186110.0416025491580.4656672276810.393446291269.9543756341-0.885383476949
182.697779763850.04025585516890.06891369064040.989947177125-0.3729811622880.228322375567-0.01414940957950.263738020507-0.630815040594-0.0441952005888-0.102952932108-0.1354892513460.3233456880830.202453509208-0.00566197421410.3038066940960.0277784036269-0.04557852622750.336935564534-0.1086738374740.45760494107716.893022100349.370264408223.9237964702
190.06977194504430.0593566070408-0.164733179960.234364972569-0.2529698034980.3089953290740.509325472048-1.00085282629-0.2353982335220.334630737987-0.298020373910.173592600115-0.2251703243190.121198048215-2.89821911351E-60.419444682386-0.06701539037910.0386512113490.423932376310.009235788695910.3673496935837.6760664931854.97357529142.4126744524
200.6327205052670.09549074543150.7588032495530.169845217931-0.03836393307810.6557820869540.0232225006169-0.4472004684980.5298770986780.0569137846629-0.102893157574-0.132099200711-0.131411633972-0.150800812406-4.20236640201E-80.3749354487070.0326295023315-0.01876321151560.27423632312-0.056422399610.36434163526615.975013204273.408104712438.1580726155
210.403920544858-0.5634821932340.0302278811661.295581307510.5641978954840.729029223083-0.2202381432210.2909618570350.2446292790850.02471509366740.171904710613-0.184251419002-0.3849051007670.397631193523-2.02586752207E-80.404202052832-0.0193945081921-0.02210661878080.384419072425-0.02668771446490.38657544717633.972925937467.421115542635.9155463578
222.01092052262-0.08210375814970.04430738915460.202744711078-0.1921285390040.187944297294-0.04320184085050.8907520886830.689447702049-0.395000078709-0.005203874847220.4576980837380.0990093483628-0.416442016283-0.03495772867110.472060116848-0.007178870486750.01490686785760.437401596096-0.07337704707280.2957444045413.2122010266167.143531735829.5468889833
230.827949179129-0.176791672891-0.5774601769911.22452191955-0.226325318930.441883251076-0.119204169625-0.0206101204461-0.131486570928-0.06229997121470.07233021314040.01391451918670.445368912371-0.01056268400444.49150124473E-70.3283660053280.028701471357-0.03526432979530.296002603829-0.0004905988537230.316156772513.0324497785651.18330087843.51049137139
240.233673886469-0.3382923910430.07270196977260.6973860808010.06215873938630.1258489622740.0904074637314-0.876856023512-0.4043210800960.2887521651960.300638942617-0.2588620345820.131212841926-1.573530011060.01197429451240.4425175503430.0259370641495-0.05504475900890.6804321729130.09893866266640.493118035844-11.973301570258.024288174821.634850576
250.114590814649-0.1239281883410.1578446093210.245954564272-0.05112128585720.22784560237-0.0463198304493-0.4565496409030.246572343297-0.01293050616380.434479657553-0.03119715790750.268315951654-0.003498344291771.63233320654E-60.3706398460480.0108451696262-0.05083198836290.3238246983630.08132573149420.426167269168-8.1867391957656.17463041664.38489171068
260.117857182563-0.063617194342-0.05271074723860.0571320674314-0.007960226124820.260634694468-0.1414444415470.711933279707-0.244352069053-1.128817819270.1872177790210.9007757719190.683780984157-0.6988087921997.66380271471E-60.401878803382-0.0592109163695-0.09407080219790.421388777896-0.008685625506060.431569987852.9711114160656.477343831-9.90726678401
273.485155551962.51331573862-1.954138931912.01249019064-0.6726208756893.81411534474-0.450494365879-0.512034799555-0.01195211606430.528585240553-0.6289058673840.04036983121170.493980118360.512277485998-1.138799315220.4454875189670.02208796359510.2095154081910.1738163846820.1299692115340.65203242797710.287261162858.7971359938-15.0236264468
281.101670133880.745702394278-0.2459556656290.85487400669-0.3550368544611.102506784270.220856315589-0.3032346623060.139862382705-0.350155906430.0739025653698-0.100755024536-0.2144788261180.2592330748090.0002429055855160.3143714049160.0326915896211-0.04565602247110.3034218263480.04549978675590.2760099612274.4268758341554.4281758887.54044000806
290.433655148122-0.0296623752877-0.01612922107250.0818102536204-0.1796257072150.3152685295490.136139749048-0.06634618345370.123567135078-0.406229557155-0.00203216169683-0.5989022239580.1322948748680.4169598428391.12615977562E-70.3558970780050.04860836722780.04838472781080.3838885914630.00746522677420.39512283060717.090357353153.6451049857-3.14414958552
300.598504781434-0.214110321671-0.2408102900010.51883811875-0.2674025984450.541491391209-0.529475814722-0.8393856577071.238795218050.3540524845350.5408411164740.029085167077-0.2575506655890.324066696198-0.005256360019580.4277482757870.0179214317303-0.02918355306160.385518625417-0.001635983644230.461903949259-4.6113846778868.48848701338.49984352485
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'B' and (resid 1 through 33 )
2X-RAY DIFFRACTION2chain 'B' and (resid 34 through 47 )
3X-RAY DIFFRACTION3chain 'B' and (resid 48 through 58 )
4X-RAY DIFFRACTION4chain 'B' and (resid 59 through 73 )
5X-RAY DIFFRACTION5chain 'E' and (resid 3 through 33 )
6X-RAY DIFFRACTION6chain 'E' and (resid 34 through 47 )
7X-RAY DIFFRACTION7chain 'E' and (resid 48 through 58 )
8X-RAY DIFFRACTION8chain 'E' and (resid 59 through 69 )
9X-RAY DIFFRACTION9chain 'E' and (resid 70 through 75 )
10X-RAY DIFFRACTION10chain 'H' and (resid 2 through 28 )
11X-RAY DIFFRACTION11chain 'H' and (resid 29 through 37 )
12X-RAY DIFFRACTION12chain 'H' and (resid 38 through 55 )
13X-RAY DIFFRACTION13chain 'H' and (resid 56 through 73 )
14X-RAY DIFFRACTION14chain 'D' and (resid 2 through 33 )
15X-RAY DIFFRACTION15chain 'D' and (resid 34 through 47 )
16X-RAY DIFFRACTION16chain 'D' and (resid 48 through 58 )
17X-RAY DIFFRACTION17chain 'D' and (resid 59 through 73 )
18X-RAY DIFFRACTION18chain 'F' and (resid 2 through 58 )
19X-RAY DIFFRACTION19chain 'F' and (resid 59 through 73 )
20X-RAY DIFFRACTION20chain 'G' and (resid 1 through 33 )
21X-RAY DIFFRACTION21chain 'G' and (resid 34 through 58 )
22X-RAY DIFFRACTION22chain 'G' and (resid 59 through 76 )
23X-RAY DIFFRACTION23chain 'C' and (resid 2 through 33 )
24X-RAY DIFFRACTION24chain 'C' and (resid 34 through 47 )
25X-RAY DIFFRACTION25chain 'C' and (resid 48 through 58 )
26X-RAY DIFFRACTION26chain 'C' and (resid 59 through 68 )
27X-RAY DIFFRACTION27chain 'C' and (resid 69 through 75 )
28X-RAY DIFFRACTION28chain 'A' and (resid 1 through 37 )
29X-RAY DIFFRACTION29chain 'A' and (resid 38 through 55 )
30X-RAY DIFFRACTION30chain 'A' and (resid 56 through 74 )

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