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Open data
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Basic information
Entry | Database: PDB / ID: 6vjl | ||||||
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Title | Streptavidin mutant M112 (G26C/A46C) | ||||||
![]() | Streptavidin | ||||||
![]() | Biotin-binding protein | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Marangoni, J.M. / Wu, S.C. / Fogen, D. / Wong, S.L. / Ng, K.K.S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity. Authors: Marangoni, J.M. / Wu, S.C. / Fogen, D. / Wong, S.L. / Ng, K.K.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 45.3 KB | Display | ![]() |
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PDB format | ![]() | 28 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 321.6 KB | Display | ![]() |
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Full document | ![]() | 321.5 KB | Display | |
Data in XML | ![]() | 1.1 KB | Display | |
Data in CIF | ![]() | 3.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6vjkC ![]() 1sweS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 16714.205 Da / Num. of mol.: 1 / Mutation: G26C, A46C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-BTN / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.56 % |
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Crystal grow | Temperature: 294.15 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 28% PEG 4000, 0.15 M ammonium sulfate, 50 mM Bis-Tris |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Apr 5, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97946 Å / Relative weight: 1 |
Reflection | Resolution: 1.3→50 Å / Num. obs: 39166 / % possible obs: 99.9 % / Redundancy: 11 % / CC1/2: 1 / Rmerge(I) obs: 0.073 / Rrim(I) all: 0.076 / Rsym value: 0.073 / Net I/σ(I): 19.6 |
Reflection shell | Resolution: 1.3→1.33 Å / Redundancy: 5.6 % / Rmerge(I) obs: 0.0101 / Num. unique obs: 2809 / CC1/2: 0.577 / Rrim(I) all: 1.11 / Rsym value: 0.0101 / % possible all: 99.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1SWE Resolution: 1.3→37.35 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.954 / SU B: 0.625 / SU ML: 0.027 / Cross valid method: THROUGHOUT / ESU R: 0.046 / ESU R Free: 0.046 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.813 Å2
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Refinement step | Cycle: LAST / Resolution: 1.3→37.35 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20
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