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- PDB-6uri: HIV-1 Nef in complex with the CD4 cytoplasmic domain and the AP2 ... -

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Basic information

Entry
Database: PDB / ID: 6uri
TitleHIV-1 Nef in complex with the CD4 cytoplasmic domain and the AP2 clathrin adaptor complex
Components
  • (AP-2 complex subunit ...) x 4
  • Protein Nef
  • cDNA FLJ50658, highly similar to T-cell surface glycoprotein CD4
KeywordsVIRAL PROTEIN / CD4
Function / homology
Function and homology information


Nef mediated downregulation of CD28 cell surface expression / : / WNT5A-dependent internalization of FZD2, FZD5 and ROR2 / LDL clearance / Retrograde neurotrophin signalling / Nef Mediated CD8 Down-regulation / VLDLR internalisation and degradation / WNT5A-dependent internalization of FZD2, FZD5 and ROR2 / Trafficking of GluR2-containing AMPA receptors / WNT5A-dependent internalization of FZD4 ...Nef mediated downregulation of CD28 cell surface expression / : / WNT5A-dependent internalization of FZD2, FZD5 and ROR2 / LDL clearance / Retrograde neurotrophin signalling / Nef Mediated CD8 Down-regulation / VLDLR internalisation and degradation / WNT5A-dependent internalization of FZD2, FZD5 and ROR2 / Trafficking of GluR2-containing AMPA receptors / WNT5A-dependent internalization of FZD4 / protein trimerization / extrinsic component of presynaptic endocytic zone membrane / clathrin adaptor complex / WNT5A-dependent internalization of FZD4 / symbiont-mediated suppression of host antigen processing and presentation of peptide antigen via MHC class I / MHC class II antigen presentation / AP-2 adaptor complex / helper T cell enhancement of adaptive immune response / interleukin-16 binding / interleukin-16 receptor activity / symbiont-mediated suppression of host antigen processing and presentation of peptide antigen via MHC class II / regulation of vesicle size / postsynaptic neurotransmitter receptor internalization / clathrin coat assembly / peptidase activator activity / maintenance of protein location in cell / symbiont-mediated suppression of host apoptosis / Recycling pathway of L1 / Retrograde neurotrophin signalling / T cell selection / symbiont-mediated suppression of host autophagy / clathrin-coated endocytic vesicle / positive regulation of synaptic vesicle endocytosis / Cargo recognition for clathrin-mediated endocytosis / clathrin adaptor activity / Clathrin-mediated endocytosis / LDL clearance / MHC class II protein binding / Formation of annular gap junctions / membrane coat / vesicle budding from membrane / Gap junction degradation / clathrin-dependent endocytosis / positive regulation of kinase activity / signal sequence binding / cellular response to granulocyte macrophage colony-stimulating factor stimulus / interleukin-15-mediated signaling pathway / CD4 receptor binding / coronary vasculature development / thioesterase binding / positive regulation of monocyte differentiation / Nef Mediated CD4 Down-regulation / Alpha-defensins / endolysosome membrane / regulation of T cell activation / Other interleukin signaling / aorta development / ventricular septum development / extracellular matrix structural constituent / Neutrophil degranulation / clathrin binding / low-density lipoprotein particle receptor binding / T cell receptor complex / enzyme-linked receptor protein signaling pathway / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / MHC class I protein binding / Trafficking of GluR2-containing AMPA receptors / Recycling pathway of L1 / regulation of endocytosis / host cell Golgi membrane / macrophage differentiation / positive regulation of receptor internalization / regulation of calcium ion transport / synaptic vesicle endocytosis / Generation of second messenger molecules / T cell differentiation / EPH-ephrin mediated repulsion of cells / Co-inhibition by PD-1 / positive regulation of protein kinase activity / Binding and entry of HIV virion / negative regulation of protein localization to plasma membrane / coreceptor activity / vesicle-mediated transport / clathrin-coated pit / regulation of calcium-mediated signaling / protein serine/threonine kinase binding / viral life cycle / phosphatidylinositol binding / MHC class II antigen presentation / positive regulation of interleukin-2 production / positive regulation of calcium-mediated signaling / VLDLR internalisation and degradation / T cell activation / cell surface receptor protein tyrosine kinase signaling pathway / protein tyrosine kinase binding / kidney development / intracellular protein transport / Vpu mediated degradation of CD4 / clathrin-coated endocytic vesicle membrane
Similarity search - Function
Nef Regulatory Factor / Nef Regulatory Factor / Beta-Lactamase - #60 / AP-2 complex subunit sigma / Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain / Adaptor protein complex AP-2, alpha subunit / Alpha adaptin AP2, C-terminal domain / Mu2, C-terminal domain / AP-2 complex subunit mu, N-terminal / HIV-1 Nef protein, anchor domain superfamily ...Nef Regulatory Factor / Nef Regulatory Factor / Beta-Lactamase - #60 / AP-2 complex subunit sigma / Clathrin adaptor, alpha-adaptin, appendage, C-terminal subdomain / Adaptor protein complex AP-2, alpha subunit / Alpha adaptin AP2, C-terminal domain / Mu2, C-terminal domain / AP-2 complex subunit mu, N-terminal / HIV-1 Nef protein, anchor domain superfamily / Clathrin adaptor, beta-adaptin, appendage, Ig-like subdomain / Beta2-adaptin appendage, C-terminal sub-domain / HIV negative factor Nef / AP-1/2/4 complex subunit beta / Adaptor protein complex, sigma subunit / HIV-1 Nef protein, core domain superfamily / Negative factor, (F-Protein) or Nef / : / Beta-adaptin appendage, C-terminal subdomain / Beta2-adaptin appendage, C-terminal sub-domain / CD4, extracellular / T cell CD4 receptor C-terminal region / CD4, extracellular / T cell CD4 receptor C terminal region / T-cell surface antigen CD4 / AP complex subunit beta / Clathrin adaptor complex, small chain / Clathrin adaptor complexes small chain signature. / Clathrin adaptor complexes medium chain signature 1. / Clathrin adaptor, mu subunit / Immunoglobulin C2-set / Clathrin adaptor, mu subunit, conserved site / Clathrin adaptor complexes medium chain signature 2. / : / Immunoglobulin C2-set domain / Coatomer/calthrin adaptor appendage, C-terminal subdomain / Clathrin adaptor, alpha/beta/gamma-adaptin, appendage, Ig-like subdomain / Adaptin C-terminal domain / Adaptin C-terminal domain / AP complex, mu/sigma subunit / Clathrin adaptor complex small chain / Adaptor complexes medium subunit family / AP-2 complex subunit mu, C-terminal superfamily / Clathrin/coatomer adaptor, adaptin-like, N-terminal / Adaptin N terminal region / Mu homology domain / Mu homology domain (MHD) profile. / Clathrin adaptor, appendage, Ig-like subdomain superfamily / Longin-like domain superfamily / TBP domain superfamily / Armadillo/beta-catenin-like repeats / Armadillo / Leucine-rich Repeat Variant / Immunoglobulin / Immunoglobulin domain / Leucine-rich Repeat Variant / Beta-Lactamase / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin V-Type / Immunoglobulin V-set domain / Armadillo-like helical / Alpha Horseshoe / Immunoglobulin subtype / Immunoglobulin / Armadillo-type fold / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold / 2-Layer Sandwich / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
cDNA FLJ50658, highly similar to T-cell surface glycoprotein CD4 / T-cell surface glycoprotein CD4 / Protein Nef / AP-2 complex subunit alpha-2 / AP-2 complex subunit sigma / AP-2 complex subunit beta / AP-2 complex subunit alpha / Protein Nef / AP-2 complex subunit mu
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Homo sapiens (human)
Human immunodeficiency virus 1
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3 Å
AuthorsJia, X. / Kwon, Y.
CitationJournal: Nat.Struct.Mol.Biol. / Year: 2020
Title: Structural basis of CD4 downregulation by HIV-1 Nef.
Authors: Kwon, Y. / Kaake, R.M. / Echeverria, I. / Suarez, M. / Karimian Shamsabadi, M. / Stoneham, C. / Ramirez, P.W. / Kress, J. / Singh, R. / Sali, A. / Krogan, N. / Guatelli, J. / Jia, X.
History
DepositionOct 23, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 29, 2020Provider: repository / Type: Initial release
Revision 1.1Aug 12, 2020Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name
Revision 1.2Sep 23, 2020Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: AP-2 complex subunit alpha
S: AP-2 complex subunit sigma
N: Protein Nef
B: AP-2 complex subunit beta
M: AP-2 complex subunit mu
D: cDNA FLJ50658, highly similar to T-cell surface glycoprotein CD4


Theoretical massNumber of molelcules
Total (without water)202,0436
Polymers202,0436
Non-polymers00
Water362
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area18590 Å2
ΔGint-74 kcal/mol
Surface area65210 Å2
MethodPISA
Unit cell
Length a, b, c (Å)109.154, 109.154, 178.915
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number76
Space group name H-MP41
Space group name HallP4w
Symmetry operation#1: x,y,z
#2: -y,x,z+1/4
#3: y,-x,z+3/4
#4: -x,-y,z+1/2

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Components

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AP-2 complex subunit ... , 4 types, 4 molecules ASBM

#1: Protein AP-2 complex subunit alpha


Mass: 72069.844 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Ap2a2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q66HM2, UniProt: P18484*PLUS
#2: Protein AP-2 complex subunit sigma / Adaptor protein complex AP-2 subunit sigma / Adaptor-related protein complex 2 subunit sigma / ...Adaptor protein complex AP-2 subunit sigma / Adaptor-related protein complex 2 subunit sigma / Clathrin assembly protein 2 sigma small chain / Clathrin coat assembly protein AP17 / Clathrin coat-associated protein AP17 / HA2 17 kDa subunit / Plasma membrane adaptor AP-2 17 kDa protein / Sigma2-adaptin


Mass: 17066.701 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: AP2S1, AP17, CLAPS2 / Production host: Escherichia coli (E. coli) / References: UniProt: P53680
#4: Protein AP-2 complex subunit beta / AP105B / Adaptor protein complex AP-2 subunit beta / Adaptor-related protein complex 2 subunit beta ...AP105B / Adaptor protein complex AP-2 subunit beta / Adaptor-related protein complex 2 subunit beta / Beta-2-adaptin / Beta-adaptin / Clathrin assembly protein complex 2 beta large chain / Plasma membrane adaptor HA2/AP2 adaptin beta subunit


Mass: 69473.812 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: AP2B1, ADTB2, CLAPB1 / Production host: Escherichia coli (E. coli) / References: UniProt: P63010
#5: Protein AP-2 complex subunit mu / AP-2 mu chain / Adaptin-mu2 / Adaptor protein complex AP-2 subunit mu / Adaptor-related protein ...AP-2 mu chain / Adaptin-mu2 / Adaptor protein complex AP-2 subunit mu / Adaptor-related protein complex 2 subunit mu / Clathrin assembly protein complex 2 mu medium chain / Clathrin coat assembly protein AP50 / Clathrin coat-associated protein AP50 / HA2 50 kDa subunit / Plasma membrane adaptor AP-2 50 kDa protein


Mass: 15518.836 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: AP2M1, CLAPM1, KIAA0109 / Production host: Escherichia coli (E. coli) / References: UniProt: Q96CW1

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Protein , 2 types, 2 molecules ND

#3: Protein Protein Nef / 3'ORF / Negative factor / F-protein


Mass: 21032.518 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus 1 / Gene: nef / Production host: Escherichia coli (E. coli) / References: UniProt: Q90VU7, UniProt: P03406*PLUS
#6: Protein cDNA FLJ50658, highly similar to T-cell surface glycoprotein CD4 / cDNA / FLJ79360 / highly similar to T-cell surface glycoprotein CD4 / FLJ79361


Mass: 6881.758 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: B4DT49, UniProt: P01730*PLUS

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Non-polymers , 1 types, 2 molecules

#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3 Å3/Da / Density % sol: 58.94 % / Description: Rods
Crystal growTemperature: 298 K / Method: microbatch / pH: 6.9
Details: 15% Peg 4000, 0.2M potassium chloride, ~10% 1,6-hexanediol, 0.1M HEPES

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NSLS-II / Beamline: 17-ID-2 / Wavelength: 0.9793 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: May 8, 2019
RadiationMonochromator: Si 111 double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9793 Å / Relative weight: 1
ReflectionResolution: 3→50 Å / Num. obs: 41865 / % possible obs: 100 % / Redundancy: 16 % / Biso Wilson estimate: 90.23 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.287 / Rpim(I) all: 0.074 / Rrim(I) all: 0.296 / Χ2: 1.01 / Net I/σ(I): 11.8
Reflection shellResolution: 3→3.11 Å / Redundancy: 15.9 % / Rmerge(I) obs: 0.081 / Mean I/σ(I) obs: 1.4 / Num. unique obs: 4189 / CC1/2: 0.366 / Rpim(I) all: 0.021 / Rrim(I) all: 0.084 / Χ2: 0.999 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX1.15.2_3472refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2XA7, 4EMZ

2xa7

4emz


Resolution: 3→48.82 Å / SU ML: 0.3956 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 30.5837
RfactorNum. reflection% reflection
Rfree0.2768 1018 2.43 %
Rwork0.241 --
obs0.2419 41859 99.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 118.3 Å2
Refinement stepCycle: LAST / Resolution: 3→48.82 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12261 0 0 2 12263
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.004712488
X-RAY DIFFRACTIONf_angle_d0.875416921
X-RAY DIFFRACTIONf_chiral_restr0.05031957
X-RAY DIFFRACTIONf_plane_restr0.00562164
X-RAY DIFFRACTIONf_dihedral_angle_d4.06447634
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3-3.160.33841470.31485806X-RAY DIFFRACTION99.97
3.16-3.360.32631450.30655842X-RAY DIFFRACTION100
3.36-3.620.28251340.26045812X-RAY DIFFRACTION100
3.62-3.980.25931500.23645819X-RAY DIFFRACTION100
3.98-4.550.24281440.21185847X-RAY DIFFRACTION100
4.55-5.740.26471490.23095830X-RAY DIFFRACTION100
5.74-48.820.28781490.23535885X-RAY DIFFRACTION99.88
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.907670864640.0535619606010.9817012224941.875486648730.6973038004594.08531217673-0.07806291126860.297394703843-0.6049505844580.06553101819380.483758811548-0.5167650305270.8652613447661.12762575358-0.336858440980.7729278458520.2381722995310.1454047176620.975499345212-0.2537411041880.71824524361150.0230199156-25.0641292765-3.3266586192
24.870803734213.31605073558-2.862594350884.27058336941-1.821333726434.721231092050.5746886358610.4748070838060.4450201013620.256788610931-0.134583974270.107625352887-0.919698915796-0.939690244166-0.3581066798970.9350563029930.28145267530.1059922817850.546720875206-0.02812784778970.49485519927247.271658651411.3436054737-41.0925620413
33.35078275118-1.19481048052-4.780294934927.915127541414.267020531738.29015600056-0.5374479758520.6310695775880.289658680823-0.4805103883480.403080833739-1.158934464890.2201995922531.495845486070.1003977163920.4754888917520.05732295247820.08024190467691.13876010275-0.1543653812250.66610905184749.6387465026-10.4816873783-0.479235097963
42.07453592943-2.823237229322.481679516943.71389884232-0.04187379023723.947351193390.4965129607840.7701570496210.726080568081-0.147125121552-0.42267126914-0.0384183976717-0.08076722473660.295983107972-0.0507044635870.9566295966330.01765508975410.2456468161720.887922677283-0.1895561198830.56413251058644.1049684535-2.93871415693-5.08217895448
58.419018683120.876942086032-9.284756573094.66722125512-3.559554111422.01309670839-0.2311244028182.18842673988-0.530985199683-0.9080086216870.932362052439-2.618376846770.3778163459131.17095376762-0.8806854872780.9314349524790.07084776022810.1956300211221.83762065578-0.2885518193391.1018186195545.6212155412-7.02014842814-13.2781135428
63.18158283291-5.65729486225-2.574234554882.069431382056.320905618335.99968836569-0.305702544587-0.606168692337-0.195663631284-0.3626069030780.6028143546240.448290961839-0.1504662122631.25724840354-0.2499592766290.6089788148110.0676236472970.1609566634240.798220159713-0.1607653886410.55163341520540.0016637193-10.9110460265-3.70388230413
78.526742573788.05486675052-3.248647542729.85031473014-5.159347030393.15550241963-0.5715429631642.09963042285-0.700184471127-2.890079139820.626854605675-0.9670339112831.25199846120.938063972133-0.1496301565771.13197498179-0.08199749770510.2388149064161.48149710912-0.4864414637320.58661391769946.0737358164-17.4328441586-15.6925149286
84.970051277340.5202913657362.044057529975.555295042692.536852813937.07261653017-0.520766928103-0.79224823731-0.5190963797540.6056188061590.2858159741060.02998731213150.7528949782360.4765082715150.2554558449690.6583743882270.2216756564070.1056117290010.793426284925-0.09125429019990.42381581278540.871994409-19.40849194516.44057873666
99.597433305612.919163237066.01566427068.41529953953-1.647002223525.199361674420.770699605776-0.514946660407-1.215970454470.588003452499-0.502399691283-0.03975060170690.7265163219810.376118144221-0.2347295133270.9587174825520.2829053557670.2124554575980.789884927691-0.1446667558170.66322947242345.3306701567-29.694650496.23850102816
102.017014033165.48525299649-7.049499981981.995946410951.913233762031.98316868530.0732336033739-0.9954478431380.761119588309-0.8480179025181.334628434790.553239843626-1.248798295212.57501083168-1.412176732262.43422899211-0.11591811232-0.4929482581222.0510489886-0.1363118201151.56546392086.78900331387-34.8517832239-15.7796015493
117.353382103033.480279123925.568426350227.00049565264-1.868630984366.579900378030.187704512984-0.559695940132-0.432694170478-0.04166090348650.067083086925-0.07799019282590.6804567910190.0144255494657-0.3896010874760.6249404472880.02840494833950.1340385203610.836690664159-0.02854370687180.6501716976548.77905449495-24.59058090321.84825232368
128.740793225374.788403627844.230838338254.651849151371.708976882564.7510922514-0.599978651019-0.1355285889290.287653074998-0.3923589368170.3952127569390.346607901789-0.1037576730080.007050306116840.2326839228140.5419414403790.07269370930040.1016158156090.639425639806-0.07824947874330.5052308474315.2705374358-13.2134043755-0.302475357449
135.679834542295.429900282090.07109256544219.086522403425.738547487888.55533358898-0.5405297226960.255442717380.606636836844-1.738675961251.070335152541.05237201185-0.676540621309-0.471958228481-0.2617554664490.8678791383890.168345972445-0.1039368871191.636384359610.2811593001281.081129243334.49734038436-10.2807865846-15.1898724635
141.067648292910.484215082910.04672501941217.19391919986-7.609100436655.90486532355-0.160871472099-0.02927867153080.5002390860380.870578113090.5043453724950.745928974529-0.137963596219-1.19081540714-0.2904962481771.719293705340.1796541925980.2200981345951.52238965123-0.4281881756011.0909521566310.302962214716.7968586954-9.90011559888
153.512931993040.5335856292490.01773581336442.21882365727-1.29752355861-0.0925676491338-0.1943566300370.1809050834750.4506815749730.9162950864870.1462263328220.2153504180620.0187512824898-0.348327584832-0.0262576071013.10838936027-0.3061644240750.5930155580461.48041644067-0.3763051659871.1783134025441.276821227642.1670388323-17.5354192091
162.638471246570.565034414718-0.324084956644.05227432063-0.2152136405973.852393381260.90671178493-0.8344426582180.4276240057731.92395214426-0.740968159645-0.283684786548-0.4778822720750.680698985469-0.2344146993731.78148954574-0.4679172759180.05114972413780.727441460501-0.1082736504080.68982425376974.463946885820.2367720198-28.9328961427
174.29896784796-0.945672742582-3.95161341991.063160206670.9681401090924.546588801480.949175033464-0.06904136723730.6558741490031.03990847008-0.3749973216350.299238727849-1.82618538813-0.351643162964-0.6610723332652.96030492296-0.1450518190720.8124540502270.952358213715-0.115035331731.368246109357.950524597430.8886669234-32.0126476512
184.54548777899-0.4388620469563.221089212822.006521546571.990373870279.54129855013.02025658021.35954067763-0.607419352296-1.91789660401-2.526268472870.3302911310510.8699547736041.36732534068-0.003990439488452.791207821620.9526320795711.390036517811.613792525210.2247427434731.7904181277533.448190844520.5403908503-9.01992618237
197.150741732541.675917269827.819599730126.330451808641.049929010858.816260019160.603424310306-2.770241951081.243772320551.90142666766-0.572191743416-0.648910181357-1.18148679928-2.22507339845-0.07280185905431.69759070236-0.2147071897030.3794727806691.39294675347-0.1952880585191.1867249330534.362763816813.1734423362-1.93390166812
205.794309929496.555245953342.632542849647.641114372974.372540451249.371774576070.998149568859-0.638302231989-1.51438674656-2.68573887142-0.612289491298-0.2104271777861.63465914922-0.6111036919830.1129489980051.96162675288-0.05237602731340.1324730892141.17467781352-0.5927158887492.209220135734.781050573313.2836546604-13.2425790849
211.209683283121.00996994969-0.126224532770.938442752809-0.0975609064340.00192148015193-0.07237917616370.0503972011576-1.082825105190.387016280181.16120037093-0.916404168235-0.5227341701121.32417951859-0.9587556702332.82493005376-0.1346672564890.4325480727061.4176900346-0.5288331663691.5232216397446.24593600417.5179771761-12.6525904741
226.0735803871-2.604604094118.003058382783.66222404386-2.664032924811.97696470144-1.336558027290.0696316889141-1.95383121522-0.457188851036-1.04868163426-0.80822810794-0.388068248751-2.592049458221.929660574062.144754397180.403573929020.8631141881671.31339059387-0.0766390509951.6950554379441.809118691121.8952045887-7.83189405955
238.40645177788-2.43456157897-0.6291141015162.15145737815-0.1763987990040.0956596118841-0.157252054275-0.434404158773-0.3012851774520.3823871794261.26895454736-0.480040842906-3.1597173050.569838482748-1.00878822282.3591881073-0.1547704511590.3882157390090.926789787504-0.3154649996630.97492875372638.610547177122.5326423696-9.86783280532
245.36688790822-2.574901346192.572060045426.8830641163-5.584017115654.601635696960.63217858494-3.0749130811-0.720888886817-1.570603797911.37957700831-0.827290299423-1.828312377280.0286400655763-1.082821815452.78521621114-0.004002830023990.8657239620840.968891357849-0.4567845352091.1030120499733.988852279425.26062863481.8180006403
251.51539878022.581149414740.4045689107966.684031881030.9146383237570.110307320477-0.350676281768-0.307939990454-0.269716464757-1.450346570590.1199529427761.78302817174-0.372938631078-0.6848511094830.4141138338193.662791376970.8366908804480.7489561778091.48473821393-0.07078760438721.8512911191724.741419584627.5028505641-5.85015106684
261.998340521247.507726390047.052538089254.64646884424.47227385516.6544125414-0.9791568964364.01536251605-2.89832278027-0.7513863784860.1603439115450.0923218446775-0.6634196667870.5185713331270.8016327696791.020432451220.352428991867-0.1492485316291.72684722907-0.6241462017741.717315705430.0443316528099-22.182847505-11.1027422557
271.99214724569-2.516245634119.870451744560.113079565853-0.5397010563532.1077689786-0.9843672590682.03887820822-0.0476450184931-1.253322733841.98087662992-1.432106848290.09194541471491.54540229713-0.8709520917990.8545626539050.600653781465-1.795898994272.73678264388-2.240597140462.83278266811.63566539187-29.4065786668-12.8253805113
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 9 through 358 )
2X-RAY DIFFRACTION2chain 'A' and (resid 359 through 619 )
3X-RAY DIFFRACTION3chain 'S' and (resid 1 through 24 )
4X-RAY DIFFRACTION4chain 'S' and (resid 25 through 48 )
5X-RAY DIFFRACTION5chain 'S' and (resid 49 through 54 )
6X-RAY DIFFRACTION6chain 'S' and (resid 55 through 71 )
7X-RAY DIFFRACTION7chain 'S' and (resid 72 through 76 )
8X-RAY DIFFRACTION8chain 'S' and (resid 77 through 117 )
9X-RAY DIFFRACTION9chain 'S' and (resid 118 through 142 )
10X-RAY DIFFRACTION10chain 'N' and (resid 34 through 52 )
11X-RAY DIFFRACTION11chain 'N' and (resid 53 through 103 )
12X-RAY DIFFRACTION12chain 'N' and (resid 104 through 191 )
13X-RAY DIFFRACTION13chain 'N' and (resid 192 through 203 )
14X-RAY DIFFRACTION14chain 'B' and (resid 13 through 134 )
15X-RAY DIFFRACTION15chain 'B' and (resid 135 through 349 )
16X-RAY DIFFRACTION16chain 'B' and (resid 350 through 532 )
17X-RAY DIFFRACTION17chain 'B' and (resid 533 through 582 )
18X-RAY DIFFRACTION18chain 'M' and (resid 1 through 8 )
19X-RAY DIFFRACTION19chain 'M' and (resid 9 through 19 )
20X-RAY DIFFRACTION20chain 'M' and (resid 20 through 35 )
21X-RAY DIFFRACTION21chain 'M' and (resid 36 through 52 )
22X-RAY DIFFRACTION22chain 'M' and (resid 53 through 60 )
23X-RAY DIFFRACTION23chain 'M' and (resid 61 through 74 )
24X-RAY DIFFRACTION24chain 'M' and (resid 75 through 105 )
25X-RAY DIFFRACTION25chain 'M' and (resid 106 through 124 )
26X-RAY DIFFRACTION26chain 'D' and (resid 406 through 411 )
27X-RAY DIFFRACTION27chain 'D' and (resid 412 through 419 )

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