A: Kanamycin B dioxygenase B: Kanamycin B dioxygenase C: Kanamycin B dioxygenase D: Kanamycin B dioxygenase E: Kanamycin B dioxygenase F: Kanamycin B dioxygenase hetero molecules
Mass: 18.015 Da / Num. of mol.: 757 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 5.3 Å3/Da / Density % sol: 76.83 %
Crystal grow
Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1:1 of 28% PEG4000 (w/v), 0.5 M lithium sulfate, 0.1 M HEPES, 0.1 M sodium acetate and 15mg/ml protein in 0.05 M bis-tris methane, 0.15 M NaCl
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N