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Open data
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Basic information
Entry | Database: PDB / ID: 4u5i | |||||||||
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Title | Complex structure of mutant CtCel5E (E314A) with xylobiose | |||||||||
![]() | Endoglucanase H | |||||||||
![]() | HYDROLASE / bi-functional cellulase/xylanase | |||||||||
Function / homology | ![]() cellulase / cellulase activity / beta-glucosidase activity / cellulose catabolic process / cell surface / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Guo, R.T. / Huang, C.H. / Wu, T.H. | |||||||||
![]() | ![]() Title: Biochemical Characterization and Structural Analysis of a Bifunctional Cellulase/Xylanase from Clostridium thermocellum Authors: Yuan, S.F. / Wu, T.H. / Lee, H.L. / Hsieh, H.Y. / Lin, W.L. / Yang, B. / Chang, C.K. / Li, Q. / Gao, J. / Huang, C.H. / Ho, M.C. / Guo, R.T. / Liang, P.H. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 144.4 KB | Display | ![]() |
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PDB format | ![]() | 110.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 972.1 KB | Display | ![]() |
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Full document | ![]() | 987.2 KB | Display | |
Data in XML | ![]() | 27.6 KB | Display | |
Data in CIF | ![]() | 38.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4u3aSC ![]() 4u5kC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 46192.414 Da / Num. of mol.: 2 / Fragment: UNP residues 290-654 / Mutation: E314A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: celH, Cthe_1472 / Plasmid: pHTPP13 / Production host: ![]() ![]() #2: Polysaccharide | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 36.94 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: 0.2 M ammonium acetate, 0.1 M sodium acetate and 21% (W/V) PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 24, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→25 Å / Num. obs: 26220 / % possible obs: 98.8 % / Redundancy: 4.9 % / Rsym value: 0.082 / Net I/σ(I): 23.1 |
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 5.2 % / Rmerge(I) obs: 0.513 / Mean I/σ(I) obs: 3.3 / % possible all: 100 |
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Processing
Software | Name: CNS / Version: 1.21 / Classification: refinement | ||||||||||||||||||||
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Refinement | Method to determine structure: ![]() Starting model: 4U3A Resolution: 2.5→25 Å / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 2.5→25 Å
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