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- PDB-6rxt: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaet... -

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Entry
Database: PDB / ID: 6rxt
TitleCryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state A
Components
  • (40S ribosomal protein ...) x 10
  • (Putative U3 small nucleolar ...) x 2
  • 35S ribosomal RNA
  • Bms1
  • Emg1
  • Enp1
  • Faf1
  • Fcf2
  • Imp3
  • KRR1 small subunit processome component
  • Kre33
  • Noc4
  • Nop1
  • Nop58
  • Periodic tryptophan protein 2-like protein
  • Pre-rRNA-processing protein PNO1
  • Putative U3 snoRNP protein
  • Putative nucleolar proteinNucleolus
  • Putative ribosomal protein
  • RNA 3'-terminal phosphate cyclase-like protein
  • Rrp9
  • Sof1
  • U3 small nucleolar RNA-associated protein 11
  • U3 snoRNA
  • UTP10
  • Utp12
  • Utp13"
  • Utp14
  • Utp15
  • Utp16
  • Utp17
  • Utp18
  • Utp2
  • Utp24
  • Utp30
  • Utp3
  • Utp4
  • Utp5"
  • Utp6
  • Utp7
  • Utp8
  • snu13NHP2L1
KeywordsRIBOSOME / ribosome biogenesis / rRNA
Function / homology
Function and homology information


rRNA cytidine N-acetyltransferase activity / tRNA acetylation / mRNA modification / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear microtubule / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex ...rRNA cytidine N-acetyltransferase activity / tRNA acetylation / mRNA modification / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear microtubule / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / box C/D RNP complex / snRNA binding / rRNA primary transcript binding / rRNA base methylation / protein localization to nucleolus / sno(s)RNA-containing ribonucleoprotein complex / U3 snoRNA binding / rRNA export from nucleus / 90S preribosome / preribosome, small subunit precursor / snoRNA binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / RNA processing / small-subunit processome / ribosomal small subunit biogenesis / nuclear periphery / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / catalytic activity / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / small ribosomal subunit rRNA binding / rRNA processing / mRNA processing / ribosome biogenesis / methyltransferase activity / ribonucleoprotein complex / cytosolic small ribosomal subunit / small ribosomal subunit / tRNA binding / ribosome / methylation / rRNA binding / structural constituent of ribosome / protein ubiquitination / translation / GTPase activity / GTP binding / nucleolus / RNA binding / membrane / ATP binding / identical protein binding / nucleus / cytoplasm
Ribosomal protein L1p/L10e family / RNA cytidine acetyltransferase NAT10 / Possible tRNA binding domain / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein S28e conserved site / Helix hairpin bin domain superfamily / tRNA (guanine-N1-)-methyltransferase, N-terminal / Alpha/beta knot methyltransferases / S-adenosyl-L-methionine-dependent methyltransferase / 50S ribosomal protein L30e-like ...Ribosomal protein L1p/L10e family / RNA cytidine acetyltransferase NAT10 / Possible tRNA binding domain / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein S28e conserved site / Helix hairpin bin domain superfamily / tRNA (guanine-N1-)-methyltransferase, N-terminal / Alpha/beta knot methyltransferases / S-adenosyl-L-methionine-dependent methyltransferase / 50S ribosomal protein L30e-like / Bms1/Tsr1-type G domain / TmcA/NAT10/Kre33 / Ribosomal protein S8 superfamily / 30s ribosomal protein S13, C-terminal / Nop domain superfamily / WD40-repeat-containing domain superfamily / RNA 3'-terminal phosphate cyclase, insert domain superfamily / K Homology domain, type 1 superfamily / Ribosomal protein S7 domain superfamily / Ribosomal protein S11 superfamily / RNA-binding S4 domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / Ribosome biogenesis protein Bms1, N-terminal / Ribosomal RNA-processing protein Rrp9-like / Ribosome biogenesis protein Bms1/Tsr1 / 40S ribosomal protein S1/3, eukaryotes / P-loop containing nucleoside triphosphate hydrolase / Pre-rRNA-processing protein PNO1-like / WD40 repeat, conserved site / Ribosomal protein L1, 3-layer alpha/beta-sandwich / RNA 3'-terminal phosphate cyclase type 2 / WD40-repeat-containing domain / Ribosomal protein S4, conserved site / Ribosomal S11, conserved site / Ribosomal protein S13, conserved site / Ribosomal protein S3Ae, conserved site / Pyrrolo-quinoline quinone beta-propeller repeat / Ribosomal protein L7Ae/L8/Nhp2 family / Sas10 C-terminal domain / U3 small nucleolar RNA-associated protein 15, C-terminal / G-protein beta WD-40 repeat / Nucleolar complex protein 4 / Ribosomal protein S5 domain 2-type fold / Ribosomal protein S9, conserved site / Ribosomal protein S7, conserved site / Fibrillarin, conserved site / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosomal protein S4/S9 / Ribosomal protein S15P / Ribosomal protein L1-like / RNA 3'-terminal phosphate cyclase domain / Ribosomal RNA assembly KRR1 / Periodic tryptophan protein 2 / Fcf2/DNTTIP2 / U3 small nucleolar RNA-associated protein 10 / WD40/YVTN repeat-like-containing domain superfamily / Sas10/Utp3/C1D family / Ribosomal protein S15 / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S11 / Ribosomal protein S9/S16 / Ribosomal protein S13/S18 / Ribosomal protein S8 / Ribosomal protein S12/S23 / Ribosomal protein S28e / Dip2/Utp12 Family / Nop14-like family / Sas10 C-terminal domain / Ribosomal protein S7p/S5e / U3 small nucleolar RNA-associated protein 6 / BING4CT (NUC141) domain / BP28CT (NUC211) domain / U3 small nucleolar RNA-associated protein 10 / Utp11 protein / Utp13 specific WD40 associated domain / Utp14 protein / UTP15 C terminal / U3 snoRNA associated / CBF/Mak21 family / Utp21 specific WD40 associated putative domain / Ribosomal protein S7e / Ribosomal S3Ae family / WD repeat-containing protein WDR46/Utp7 / AARP2CN (NUC121) domain / Krr1, KH1 domain / Nop, C-terminal domain / U3 snoRNP protein/Ribosome production factor 1 / Fibrillarin / snoRNA binding domain, fibrillarin / NOP5NT (NUC127) domain / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / WD domain, G-beta repeat / RNA 3'-terminal phosphate cyclase / RNA 3'-terminal phosphate cyclase (RTC), insert domain / 40S ribosome biogenesis protein Tsr1 and BMS1 C-terminal / Ribosomal protein S4/S9 N-terminal domain / Bystin / S4 domain / Brix domain / Mpp10 protein
CBF domain-containing protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / RNA cytidine acetyltransferase / Utp12 domain-containing protein / 40S ribosomal protein S13-like protein / Nucleolar protein 58 / DDB1- and CUL4-associated factor 13 / 40S ribosomal protein s23-like protein / RNA 3'-terminal phosphate cyclase-like protein ...CBF domain-containing protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / RNA cytidine acetyltransferase / Utp12 domain-containing protein / 40S ribosomal protein S13-like protein / Nucleolar protein 58 / DDB1- and CUL4-associated factor 13 / 40S ribosomal protein s23-like protein / RNA 3'-terminal phosphate cyclase-like protein / 40S ribosomal protein s9-like protein / 40S ribosomal protein s5-like protein / Putative ribosomal protein / Uncharacterized protein / Nucleolar protein 58 / WD_REPEATS_REGION domain-containing protein / Putative U3 small nucleolar ribonucleoprotein / Uncharacterized protein / S4 RNA-binding domain-containing protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / 40S ribosomal protein S16-like protein / Uncharacterized protein / U3 small nucleolar RNA-associated protein 11 / 40S ribosomal protein S28-like protein / Putative U3 small nucleolar ribonucleoprotein protein / Uncharacterized protein / 40S ribosomal protein S7 / Putative U3 snoRNP protein / Uncharacterized protein / WD_REPEATS_REGION domain-containing protein / Periodic tryptophan protein 2-like protein / 40S ribosomal protein S1 / U3 small nucleolar RNA-associated protein 10 / Pre-rRNA-processing protein PNO1 / KRR1 small subunit processome component / Bms1-type G domain-containing protein / Fcf2 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S14-like protein / Uncharacterized protein CTHT_0071780 / 40S ribosomal protein S22-like protein / Histone-glutamine methyltransferase / Ribonucloprotein / WD_REPEATS_REGION domain-containing protein / Uncharacterized protein / Sas10 domain-containing protein
Biological speciesChaetomium thermophilum (fungus)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 7 Å
AuthorsCheng, J. / Kellner, N. / Griesel, S. / Berninghausen, O. / Beckmann, R. / Hurt, E.
CitationJournal: Mol Cell / Year: 2019
Title: Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.
Authors: Jingdong Cheng / Jochen Baßler / Paulina Fischer / Benjamin Lau / Nikola Kellner / Ruth Kunze / Sabine Griesel / Martina Kallas / Otto Berninghausen / Daniela Strauss / Roland Beckmann / Ed Hurt /
Abstract: Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome ...Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors.
Validation Report
SummaryFull reportAbout validation report
History
DepositionJun 10, 2019Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 14, 2019Provider: repository / Type: Initial release
Revision 1.1Oct 2, 2019Group: Data collection / Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last

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Structure visualization

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Structure viewerMolecule:
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Assembly

Deposited unit
UA: Periodic tryptophan protein 2-like protein
UB: Utp2
UC: Utp3
UD: Utp4
UF: Utp6
UG: Utp7
UJ: UTP10
UK: U3 small nucleolar RNA-associated protein 11
UL: Utp12
UM: Utp13"
UN: Utp14
UO: Utp15
UQ: Utp17
UR: Utp18
UU: Putative U3 snoRNP protein
UX: Utp24
UZ: Utp30
CA: Nop1
CB: Nop1
CC: Putative nucleolar protein
CD: Nop58
CE: snu13
CF: snu13
CG: Rrp9
CH: RNA 3'-terminal phosphate cyclase-like protein
CI: Bms1
CJ: Imp3
CK: Putative U3 small nucleolar ribonucleoprotein
CL: Putative U3 small nucleolar ribonucleoprotein protein
CM: Sof1
CN: Emg1
CO: Emg1
CP: KRR1 small subunit processome component
CQ: Pre-rRNA-processing protein PNO1
CR: Kre33
CS: Kre33
CT: Fcf2
Ca: 40S ribosomal protein S1
Cc: 40S ribosomal protein s5-like protein
Ce: 40S ribosomal protein S7
Cg: 40S ribosomal protein s9-like protein
Ch: 40S ribosomal protein S13-like protein
Ci: 40S ribosomal protein S14-like protein
Cj: 40S ribosomal protein S16-like protein
Cm: 40S ribosomal protein S22-like protein
Cn: 40S ribosomal protein s23-like protein
Cp: 40S ribosomal protein S28-like protein
CU: Faf1
C1: 35S ribosomal RNA
C2: U3 snoRNA
UH: Utp8
UE: Utp5"
US: Noc4
Cl: Putative ribosomal protein
CX: Enp1
UI: Utp5"
UP: Utp16
hetero molecules


Theoretical massNumber of molelcules
Total (without water)3,905,71460
Polymers3,905,10157
Non-polymers6133
Water0
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: immunoprecipitation
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area406150 Å2
ΔGint-2424 kcal/mol
Surface area900240 Å2
MethodPISA

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Components

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Protein , 38 types, 43 molecules UAUBUCUDUFUGUJUKULUMUNUOUQURUUUXUZCACBCCCDCECFCGCHCICJCMCNCO...

#1: Protein Periodic tryptophan protein 2-like protein


Mass: 100817.508 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SF93
#2: Protein Utp2


Mass: 103960.703 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SDW0
#3: Protein Utp3 /


Mass: 73214.633 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S4H3
#4: Protein Utp4


Mass: 97696.523 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SCT7
#5: Protein Utp6 /


Mass: 46924.773 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S387
#6: Protein Utp7


Mass: 63629.379 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RZ99
#7: Protein UTP10


Mass: 198508.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S5L1
#8: Protein U3 small nucleolar RNA-associated protein 11 / U3 snoRNA-associated protein 11


Mass: 31379.650 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SF32
#9: Protein Utp12


Mass: 107573.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RZL9
#10: Protein Utp13"


Mass: 100430.352 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SG95
#11: Protein Utp14


Mass: 104741.391 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S8Y4
#12: Protein Utp15 /


Mass: 60524.145 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SEI5
#13: Protein Utp17


Mass: 105039.695 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SCS8
#14: Protein Utp18 /


Mass: 69055.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SBN9
#15: Protein Putative U3 snoRNP protein


Mass: 115122.008 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S872
#16: Protein Utp24


Mass: 22135.992 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#17: Protein Utp30


Mass: 43700.527 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S7X0
#18: Protein Nop1


Mass: 32960.844 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SGY2
#19: Protein Putative nucleolar protein / Nucleolus


Mass: 57830.699 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S2U4
#20: Protein Nop58 /


Mass: 64292.113 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RYF6
#21: Protein snu13 / NHP2L1


Mass: 13831.854 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SGM0
#22: Protein Rrp9 /


Mass: 69610.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SG51
#23: Protein RNA 3'-terminal phosphate cyclase-like protein


Mass: 43399.766 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S0B1
#24: Protein Bms1


Mass: 134172.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S445
#25: Protein Imp3 /


Mass: 21802.396 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SDL4
#28: Protein Sof1


Mass: 50869.828 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RY94
#29: Protein Emg1 /


Mass: 27936.164 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SAY9
#30: Protein KRR1 small subunit processome component / / KRR-R motif-containing protein 1


Mass: 37500.777 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S3V7
#31: Protein Pre-rRNA-processing protein PNO1


Mass: 28715.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S3T2
#32: Protein Kre33


Mass: 119795.125 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
References: UniProt: G0S273, Transferases; Acyltransferases; Transferring groups other than aminoacyl groups
#33: Protein Fcf2


Mass: 22569.129 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S4E3
#44: Protein Faf1 /


Mass: 34576.586 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S5F1
#47: Protein Utp8


Mass: 102421.133 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P0CT47
#48: Protein Utp5"


Mass: 44663.355 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RYX2
#49: Protein Noc4


Mass: 63331.664 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RZL4
#50: Protein Putative ribosomal protein /


Mass: 17826.707 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S6J7
#51: Protein Enp1


Mass: 54319.605 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S5J4
#52: Protein Utp16


Mass: 40726.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S612

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Putative U3 small nucleolar ... , 2 types, 2 molecules CKCL

#26: Protein Putative U3 small nucleolar ribonucleoprotein


Mass: 34216.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SE90
#27: Protein Putative U3 small nucleolar ribonucleoprotein protein


Mass: 86082.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S9I7

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40S ribosomal protein ... , 10 types, 10 molecules CaCcCeCgChCiCjCmCnCp

#34: Protein 40S ribosomal protein S1 /


Mass: 29245.158 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S7T8
#35: Protein 40S ribosomal protein s5-like protein / Ribosome


Mass: 23679.225 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S1Z0
#36: Protein 40S ribosomal protein S7 /


Mass: 23084.650 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S8C4
#37: Protein 40S ribosomal protein s9-like protein / Ribosome


Mass: 22029.836 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S0Z4
#38: Protein 40S ribosomal protein S13-like protein / Ribosome


Mass: 16912.891 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RZM9
#39: Protein 40S ribosomal protein S14-like protein / Ribosome


Mass: 16071.464 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SFL1
#40: Protein 40S ribosomal protein S16-like protein / Ribosome


Mass: 15962.771 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SBR7
#41: Protein 40S ribosomal protein S22-like protein / Ribosome


Mass: 14905.471 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SHI0
#42: Protein 40S ribosomal protein s23-like protein / Ribosome


Mass: 15934.653 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RY17
#43: Protein 40S ribosomal protein S28-like protein / Ribosome


Mass: 7741.980 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S9M9

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RNA chain , 2 types, 2 molecules C1C2

#45: RNA chain 35S ribosomal RNA /


Mass: 758475.312 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#46: RNA chain U3 snoRNA


Mass: 73966.539 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)

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Non-polymers , 3 types, 3 molecules

#53: Chemical ChemComp-ZN / ZINC ION / Zinc


Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn
#54: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE / Guanosine triphosphate


Mass: 523.180 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#55: Chemical ChemComp-MG / MAGNESIUM ION / Magnesium


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 90S pre-ribosome, state A / Type: RIBOSOME / Entity ID: #1-#52 / Source: NATURAL
Source (natural)Organism: Chaetomium thermophilum (fungus)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 28 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)

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Processing

EM software
IDNameCategory
4RELIONCTF correction
10RELIONinitial Euler assignment
11RELIONfinal Euler assignment
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 8415 / Symmetry type: POINT

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