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- PDB-6rxt: Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaet... -

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Basic information

Entry
Database: PDB / ID: 6rxt
TitleCryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state A
Components
  • (40S ribosomal protein ...) x 10
  • (Putative U3 small nucleolar ...) x 2
  • 35S ribosomal RNA
  • Bms1
  • Emg1
  • Enp1
  • Faf1
  • Fcf2
  • Imp3
  • KRR1 small subunit processome component
  • Kre33
  • Noc4
  • Nop1
  • Nop58
  • Periodic tryptophan protein 2-like protein
  • Pre-rRNA-processing protein PNO1
  • Putative U3 snoRNP protein
  • Putative nucleolar proteinNucleolus
  • Putative ribosomal protein
  • RNA 3'-terminal phosphate cyclase-like protein
  • Rrp9
  • Sof1
  • U3 small nucleolar RNA-associated protein 11
  • U3 snoRNA
  • UTP10
  • Utp12
  • Utp13"
  • Utp14
  • Utp15
  • Utp16
  • Utp17
  • Utp18
  • Utp2
  • Utp24
  • Utp30
  • Utp3
  • Utp4
  • Utp5"
  • Utp6
  • Utp7
  • Utp8
  • snu13
KeywordsRIBOSOME / ribosome biogenesis / rRNA
Function / homology
Function and homology information


rRNA cytidine N-acetyltransferase activity / tRNA acetylation / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / nuclear microtubule / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / snRNA binding / box C/D snoRNP complex ...rRNA cytidine N-acetyltransferase activity / tRNA acetylation / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / nuclear microtubule / rRNA modification / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / snRNA binding / box C/D snoRNP complex / rRNA base methylation / U3 snoRNA binding / protein localization to nucleolus / rRNA export from nucleus / small nucleolar ribonucleoprotein complex / preribosome, small subunit precursor / 90S preribosome / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / snoRNA binding / Transferases, Acyltransferases, Transferring groups other than aminoacyl groups / RNA processing / small-subunit processome / ribosomal small subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / nuclear periphery / small ribosomal subunit rRNA binding / methyltransferase activity / small ribosomal subunit / rRNA processing / catalytic activity / ribosome biogenesis / cytosolic small ribosomal subunit / ribonucleoprotein complex / ribosome / rRNA binding / structural constituent of ribosome / translation / GTPase activity / GTP binding / nucleolus / RNA binding / ATP binding / identical protein binding / nucleus / cytoplasm
Utp14 protein / WD repeat-containing protein WDR46/Utp7 / K Homology domain, type 1 superfamily / Ribosomal protein S7 domain superfamily / Ribosomal protein S11 superfamily / RNA-binding S4 domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / Ribosome biogenesis protein Bms1, N-terminal / Ribosomal RNA-processing protein Rrp9-like / Ribosome biogenesis protein Bms1/Tsr1 ...Utp14 protein / WD repeat-containing protein WDR46/Utp7 / K Homology domain, type 1 superfamily / Ribosomal protein S7 domain superfamily / Ribosomal protein S11 superfamily / RNA-binding S4 domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / Ribosome biogenesis protein Bms1, N-terminal / Ribosomal RNA-processing protein Rrp9-like / Ribosome biogenesis protein Bms1/Tsr1 / Fcf2/DNTTIP2 / Pre-rRNA-processing protein PNO1-like / U3 small nucleolar RNA-associated protein 10 / Krr1, KH1 domain / WD40-repeat-containing domain superfamily / Nop, C-terminal domain / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S12/S23 / Ribosomal protein S7p/S5e / Ribosomal protein S15 / Ribosomal protein S9/S16 / WD domain, G-beta repeat / Ribosomal protein S8 / Ribosomal protein S11 / Ribosomal protein S13/S18 / Ribosomal protein L1p/L10e family / Ribosomal S3Ae family / RNA 3'-terminal phosphate cyclase, insert domain superfamily / Nop domain superfamily / Ribosomal protein S28e / P-loop containing nucleoside triphosphate hydrolase / Ribosomal protein S9, conserved site / Ribosomal protein S7, conserved site / Fibrillarin, conserved site / U3 small nucleolar RNA-associated protein 10, N-terminal / Ribosomal protein S4/S9 / Ribosomal protein S15P / Ribosomal protein L1-like / RNA 3'-terminal phosphate cyclase domain / Ribosomal protein S7 domain / Ribosomal RNA assembly KRR1 / Periodic tryptophan protein 2 / Nucleolar complex protein 4 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S8 superfamily / 40S ribosomal protein S1/3, eukaryotes / Protein of unknown function DUF4602 / Possible tRNA binding domain / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein S28e conserved site / Helix hairpin bin domain superfamily / tRNA (guanine-N1-)-methyltransferase, N-terminal / Alpha/beta knot methyltransferases / 50S ribosomal protein L30e-like / Bms1/Tsr1-type G domain / TmcA/NAT10/Kre33 / RNA cytidine acetyltransferase NAT10 / RNA 3'-terminal phosphate cyclase / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family / G-protein beta WD-40 repeat / Ribosomal protein S4 signature. / GNAT acetyltransferase 2 / Possible tRNA binding domain / Domain of unknown function (DUF4602) / Krr1 KH1 domain / Ribosomal protein S7 signature. / Ribosomal protein S8 signature. / Ribosomal protein S11 signature. / Ribosomal protein S12 signature. / Ribosomal protein S9 signature. / Ribosomal protein S15 signature. / Fibrillarin signature. / Ribosomal protein S13 signature. / UTP15 C terminal / Trp-Asp (WD) repeats signature. / Ribosomal protein S7e signature. / Ribosomal protein S28e signature. / Ribosomal protein L7Ae signature. / Ribosomal protein S3Ae signature. / Trp-Asp (WD) repeats profile. / Ribosomal protein S13 family profile. / Trp-Asp (WD) repeats circular profile. / Brix domain profile. / S4 RNA-binding domain profile. / Nop domain profile. / Bms1-type guanine nucleotide-binding (G) domain profile. / U3 small nucleolar RNA-associated protein 10 / Sas10 C-terminal domain / Ribosomal protein S7e / Brix domain / Fibrillarin / S4 domain / snoRNA binding domain, fibrillarin / EMG1/NEP1 methyltransferase / CBF/Mak21 family / Utp11 protein / Sas10/Utp3/C1D family / Dip2/Utp12 Family / Mpp10 protein / Nop14-like family
S4 RNA-binding domain-containing protein / Putative U3 small nucleolar ribonucleoprotein protein / 40S ribosomal protein S28-like protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S16-like protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / Uncharacterized protein / Periodic tryptophan protein 2-like protein ...S4 RNA-binding domain-containing protein / Putative U3 small nucleolar ribonucleoprotein protein / 40S ribosomal protein S28-like protein / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein S16-like protein / WD_REPEATS_REGION domain-containing protein / WD_REPEATS_REGION domain-containing protein / Uncharacterized protein / Periodic tryptophan protein 2-like protein / Uncharacterized protein / Putative U3 small nucleolar ribonucleoprotein / WD_REPEATS_REGION domain-containing protein / U3 small nucleolar RNA-associated protein 11 / Putative U3 snoRNP protein / 40S ribosomal protein S14-like protein / Uncharacterized protein / WD_REPEATS_REGION domain-containing protein / Ribonucloprotein / Uncharacterized protein / 40S ribosomal protein S22-like protein / Uncharacterized protein CTHT_0071780 / 40S ribosomal protein S7 / Uncharacterized protein / Uncharacterized protein / 40S ribosomal protein s5-like protein / 40S ribosomal protein s23-like protein / WD_REPEATS_REGION domain-containing protein / Nop domain-containing protein / Utp12 domain-containing protein / WD_REPEATS_REGION domain-containing protein / CBF domain-containing protein / WD_REPEATS_REGION domain-containing protein / 40S ribosomal protein S13-like protein / RNA 3'-terminal phosphate cyclase-like protein / 40S ribosomal protein s9-like protein / RNA cytidine acetyltransferase / 40S ribosomal protein S1 / Putative nucleolar protein / Pre-rRNA-processing protein PNO1 / KRR1 small subunit processome component / Bms1-type G domain-containing protein / Fcf2 domain-containing protein / Sas10 domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Putative ribosomal protein / Uncharacterized protein
Biological speciesChaetomium thermophilum (fungus)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 7 Å
AuthorsCheng, J. / Kellner, N. / Griesel, S. / Berninghausen, O. / Beckmann, R. / Hurt, E.
CitationJournal: Mol. Cell / Year: 2019
Title: Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.
Authors: Jingdong Cheng / Jochen Baßler / Paulina Fischer / Benjamin Lau / Nikola Kellner / Ruth Kunze / Sabine Griesel / Martina Kallas / Otto Berninghausen / Daniela Strauss / Roland Beckmann / Ed Hurt /
Abstract: Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome ...Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors.
Validation Report
SummaryFull reportAbout validation report
History
DepositionJun 10, 2019Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 14, 2019Provider: repository / Type: Initial release

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Structure visualization

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Structure viewerMolecule:
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Assembly

Deposited unit
UA: Periodic tryptophan protein 2-like protein
UB: Utp2
UC: Utp3
UD: Utp4
UF: Utp6
UG: Utp7
UJ: UTP10
UK: U3 small nucleolar RNA-associated protein 11
UL: Utp12
UM: Utp13"
UN: Utp14
UO: Utp15
UQ: Utp17
UR: Utp18
UU: Putative U3 snoRNP protein
UX: Utp24
UZ: Utp30
CA: Nop1
CB: Nop1
CC: Putative nucleolar protein
CD: Nop58
CE: snu13
CF: snu13
CG: Rrp9
CH: RNA 3'-terminal phosphate cyclase-like protein
CI: Bms1
CJ: Imp3
CK: Putative U3 small nucleolar ribonucleoprotein
CL: Putative U3 small nucleolar ribonucleoprotein protein
CM: Sof1
CN: Emg1
CO: Emg1
CP: KRR1 small subunit processome component
CQ: Pre-rRNA-processing protein PNO1
CR: Kre33
CS: Kre33
CT: Fcf2
Ca: 40S ribosomal protein S1
Cc: 40S ribosomal protein s5-like protein
Ce: 40S ribosomal protein S7
Cg: 40S ribosomal protein s9-like protein
Ch: 40S ribosomal protein S13-like protein
Ci: 40S ribosomal protein S14-like protein
Cj: 40S ribosomal protein S16-like protein
Cm: 40S ribosomal protein S22-like protein
Cn: 40S ribosomal protein s23-like protein
Cp: 40S ribosomal protein S28-like protein
CU: Faf1
C1: 35S ribosomal RNA
C2: U3 snoRNA
UH: Utp8
UE: Utp5"
US: Noc4
Cl: Putative ribosomal protein
CX: Enp1
UI: Utp5"
UP: Utp16
hetero molecules


Theoretical massNumber of molelcules
Total (without water)3,905,71460
Polymers3,905,10157
Non-polymers6133
Water0
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: immunoprecipitation
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area406150 Å2
ΔGint-2424 kcal/mol
Surface area900240 Å2
MethodPISA

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Components

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Protein/peptide , 38 types, 43 molecules UAUBUCUDUFUGUJUKULUMUNUOUQURUUUXUZCACBCCCDCECFCGCHCICJCMCNCO...

#1: Protein/peptide Periodic tryptophan protein 2-like protein


Mass: 100817.508 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SF93
#2: Protein/peptide Utp2


Mass: 103960.703 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SDW0
#3: Protein/peptide Utp3 /


Mass: 73214.633 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S4H3
#4: Protein/peptide Utp4


Mass: 97696.523 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SCT7
#5: Protein/peptide Utp6 /


Mass: 46924.773 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S387
#6: Protein/peptide Utp7


Mass: 63629.379 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RZ99
#7: Protein/peptide UTP10


Mass: 198508.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S5L1
#8: Protein/peptide U3 small nucleolar RNA-associated protein 11 / U3 snoRNA-associated protein 11


Mass: 31379.650 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SF32
#9: Protein/peptide Utp12


Mass: 107573.039 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RZL9
#10: Protein/peptide Utp13"


Mass: 100430.352 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SG95
#11: Protein/peptide Utp14


Mass: 104741.391 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S8Y4
#12: Protein/peptide Utp15 /


Mass: 60524.145 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SEI5
#13: Protein/peptide Utp17


Mass: 105039.695 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SCS8
#14: Protein/peptide Utp18 /


Mass: 69055.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SBN9
#15: Protein/peptide Putative U3 snoRNP protein


Mass: 115122.008 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S872
#16: Protein/peptide Utp24


Mass: 22135.992 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#17: Protein/peptide Utp30


Mass: 43700.527 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S7X0
#18: Protein/peptide Nop1


Mass: 32960.844 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SGY2
#19: Protein/peptide Putative nucleolar protein / Nucleolus


Mass: 57830.699 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S2U4
#20: Protein/peptide Nop58 /


Mass: 64292.113 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RYF6
#21: Protein/peptide snu13


Mass: 13831.854 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SGM0
#22: Protein/peptide Rrp9 /


Mass: 69610.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SG51
#23: Protein/peptide RNA 3'-terminal phosphate cyclase-like protein


Mass: 43399.766 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S0B1
#24: Protein/peptide Bms1


Mass: 134172.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S445
#25: Protein/peptide Imp3 /


Mass: 21802.396 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SDL4
#28: Protein/peptide Sof1


Mass: 50869.828 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RY94
#29: Protein/peptide Emg1 /


Mass: 27936.164 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SAY9
#30: Protein/peptide KRR1 small subunit processome component / / KRR-R motif-containing protein 1


Mass: 37500.777 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S3V7
#31: Protein/peptide Pre-rRNA-processing protein PNO1


Mass: 28715.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S3T2
#32: Protein/peptide Kre33


Mass: 119795.125 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
References: UniProt: G0S273, Transferases, Acyltransferases, Transferring groups other than aminoacyl groups
#33: Protein/peptide Fcf2


Mass: 22569.129 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S4E3
#44: Protein/peptide Faf1 /


Mass: 34576.586 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S5F1
#47: Protein/peptide Utp8


Mass: 102421.133 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: P0CT47
#48: Protein/peptide Utp5"


Mass: 44663.355 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RYX2
#49: Protein/peptide Noc4


Mass: 63331.664 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RZL4
#50: Protein/peptide Putative ribosomal protein /


Mass: 17826.707 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S6J7
#51: Protein/peptide Enp1


Mass: 54319.605 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S5J4
#52: Protein/peptide Utp16


Mass: 40726.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S612

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Putative U3 small nucleolar ... , 2 types, 2 molecules CKCL

#26: Protein/peptide Putative U3 small nucleolar ribonucleoprotein


Mass: 34216.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SE90
#27: Protein/peptide Putative U3 small nucleolar ribonucleoprotein protein


Mass: 86082.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S9I7

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40S ribosomal protein ... , 10 types, 10 molecules CaCcCeCgChCiCjCmCnCp

#34: Protein/peptide 40S ribosomal protein S1 /


Mass: 29245.158 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S7T8
#35: Protein/peptide 40S ribosomal protein s5-like protein / Ribosome


Mass: 23679.225 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S1Z0
#36: Protein/peptide 40S ribosomal protein S7 /


Mass: 23084.650 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S8C4
#37: Protein/peptide 40S ribosomal protein s9-like protein / Ribosome


Mass: 22029.836 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S0Z4
#38: Protein/peptide 40S ribosomal protein S13-like protein / Ribosome


Mass: 16912.891 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RZM9
#39: Protein/peptide 40S ribosomal protein S14-like protein / Ribosome


Mass: 16071.464 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SFL1
#40: Protein/peptide 40S ribosomal protein S16-like protein / Ribosome


Mass: 15962.771 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SBR7
#41: Protein/peptide 40S ribosomal protein S22-like protein / Ribosome


Mass: 14905.471 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0SHI0
#42: Protein/peptide 40S ribosomal protein s23-like protein / Ribosome


Mass: 15934.653 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0RY17
#43: Protein/peptide 40S ribosomal protein S28-like protein / Ribosome


Mass: 7741.980 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus) / References: UniProt: G0S9M9

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RNA chain , 2 types, 2 molecules C1C2

#45: RNA chain 35S ribosomal RNA /


Mass: 758475.312 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)
#46: RNA chain U3 snoRNA


Mass: 73966.539 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Chaetomium thermophilum (fungus)

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Non-polymers , 3 types, 3 molecules

#53: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn / Zinc
#54: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Guanosine triphosphate / Comment: GTP (energy-carrying molecule) *YM
#55: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg / Magnesium

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: 90S pre-ribosome, state A / Type: RIBOSOME
Entity ID: 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52
Source: NATURAL
Source (natural)Organism: Chaetomium thermophilum (fungus)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 28 e/Å2 / Film or detector model: FEI FALCON II (4k x 4k)

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Processing

EM software
IDNameCategory
4RELIONCTF correction
10RELIONinitial Euler assignment
11RELIONfinal Euler assignment
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 8415 / Symmetry type: POINT

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