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- PDB-6p4f: Crystal structure of the XPB-Bax1-forked DNA ternary complex -

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Basic information

Entry
Database: PDB / ID: 6p4f
TitleCrystal structure of the XPB-Bax1-forked DNA ternary complex
Components
  • DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*AP*AP*CP*AP*TP*CP*CP*TP*TP*TP*GP*CP*TP*AP*CP*AP*A)-3')
  • DNA (5'-D(P*TP*GP*TP*AP*GP*GP*TP*TP*TP*CP*CP*AP*TP*GP*TP*TP*GP*AP*GP*TP*CP*A)-3')
  • DNA-dependent ATPase XPBII
  • Endonuclease Bax1
KeywordsHYDROLASE/DNA / StXPB:Bax1 complex / helicase / endonuclease / ATPase / HYDROLASE-DNA complex
Function / homology
Function and homology information


catalytic activity, acting on DNA / DNA conformation change / DNA 3'-5' helicase / isomerase activity / helicase activity / endonuclease activity / Hydrolases; Acting on ester bonds / hydrolase activity / DNA repair / DNA binding / ATP binding
Similarity search - Function
Protein of unknown function DUF790, endonuclease-like / Protein of unknown function (DUF790) / Xeroderma pigmentosum group B helicase, damage recognition domain / Xeroderma pigmentosum group B helicase damage recognition domain / : / Helicase/UvrB, N-terminal / Type III restriction enzyme, res subunit / Helicase conserved C-terminal domain / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. ...Protein of unknown function DUF790, endonuclease-like / Protein of unknown function (DUF790) / Xeroderma pigmentosum group B helicase, damage recognition domain / Xeroderma pigmentosum group B helicase damage recognition domain / : / Helicase/UvrB, N-terminal / Type III restriction enzyme, res subunit / Helicase conserved C-terminal domain / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
PHOSPHATE ION / DNA / DNA (> 10) / Endonuclease Bax1 / DNA 3'-5' translocase XPB2
Similarity search - Component
Biological speciesSulfurisphaera tokodaii (archaea)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.55 Å
AuthorsHe, F. / Hilario, E. / Fan, L.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01GM108893 United States
CitationJournal: Nucleic Acids Res. / Year: 2020
Title: Structural basis of the XPB helicase-Bax1 nuclease complex interacting with the repair bubble DNA.
Authors: He, F. / DuPrez, K. / Hilario, E. / Chen, Z. / Fan, L.
History
DepositionMay 27, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 17, 2020Provider: repository / Type: Initial release
Revision 1.1Dec 30, 2020Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA-dependent ATPase XPBII
B: Endonuclease Bax1
C: DNA-dependent ATPase XPBII
D: Endonuclease Bax1
E: DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*AP*AP*CP*AP*TP*CP*CP*TP*TP*TP*GP*CP*TP*AP*CP*AP*A)-3')
F: DNA (5'-D(P*TP*GP*TP*AP*GP*GP*TP*TP*TP*CP*CP*AP*TP*GP*TP*TP*GP*AP*GP*TP*CP*A)-3')
G: DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*AP*AP*CP*AP*TP*CP*CP*TP*TP*TP*GP*CP*TP*AP*CP*AP*A)-3')
H: DNA (5'-D(P*TP*GP*TP*AP*GP*GP*TP*TP*TP*CP*CP*AP*TP*GP*TP*TP*GP*AP*GP*TP*CP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)220,09547
Polymers218,8128
Non-polymers1,28339
Water00
1
A: DNA-dependent ATPase XPBII
B: Endonuclease Bax1
E: DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*AP*AP*CP*AP*TP*CP*CP*TP*TP*TP*GP*CP*TP*AP*CP*AP*A)-3')
F: DNA (5'-D(P*TP*GP*TP*AP*GP*GP*TP*TP*TP*CP*CP*AP*TP*GP*TP*TP*GP*AP*GP*TP*CP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)110,28030
Polymers109,4064
Non-polymers87426
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10570 Å2
ΔGint-146 kcal/mol
Surface area45570 Å2
MethodPISA
2
C: DNA-dependent ATPase XPBII
D: Endonuclease Bax1
G: DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*AP*AP*CP*AP*TP*CP*CP*TP*TP*TP*GP*CP*TP*AP*CP*AP*A)-3')
H: DNA (5'-D(P*TP*GP*TP*AP*GP*GP*TP*TP*TP*CP*CP*AP*TP*GP*TP*TP*GP*AP*GP*TP*CP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)109,81517
Polymers109,4064
Non-polymers40913
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area9940 Å2
ΔGint-129 kcal/mol
Surface area45970 Å2
MethodPISA
Unit cell
Length a, b, c (Å)214.687, 92.150, 172.128
Angle α, β, γ (deg.)90.000, 132.240, 90.000
Int Tables number5
Space group name H-MC121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 1 through 2 or (resid 3...
21(chain C and (resid 1 through 29 or (resid 30...
12(chain B and (resid 0 through 49 or resid 51...
22(chain D and (resid 0 through 18 or (resid 19...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111SERSERVALVAL(chain A and (resid 1 through 2 or (resid 3...AA1 - 22 - 3
121TYRTYRARGARG(chain A and (resid 1 through 2 or (resid 3...AA3 - 54 - 6
131GLYGLYSERSER(chain A and (resid 1 through 2 or (resid 3...AA0 - 4341 - 435
211SERSERTYRTYR(chain C and (resid 1 through 29 or (resid 30...CC1 - 292 - 30
221SERSERALAALA(chain C and (resid 1 through 29 or (resid 30...CC30 - 3131 - 32
231GLYGLYARGARG(chain C and (resid 1 through 29 or (resid 30...CC0 - 4351 - 436
241GLYGLYARGARG(chain C and (resid 1 through 29 or (resid 30...CC0 - 4351 - 436
251GLYGLYARGARG(chain C and (resid 1 through 29 or (resid 30...CC0 - 4351 - 436
261GLYGLYARGARG(chain C and (resid 1 through 29 or (resid 30...CC0 - 4351 - 436
112METMETILEILE(chain B and (resid 0 through 49 or resid 51...BB0 - 491 - 50
122TYRTYRGLUGLU(chain B and (resid 0 through 49 or resid 51...BB51 - 7552 - 76
132LEULEUASPASP(chain B and (resid 0 through 49 or resid 51...BB76 - 7777 - 78
142METMETGLUGLU(chain B and (resid 0 through 49 or resid 51...BB0 - 3721 - 373
152METMETGLUGLU(chain B and (resid 0 through 49 or resid 51...BB0 - 3721 - 373
162METMETGLUGLU(chain B and (resid 0 through 49 or resid 51...BB0 - 3721 - 373
172METMETGLUGLU(chain B and (resid 0 through 49 or resid 51...BB0 - 3721 - 373
212METMETPROPRO(chain D and (resid 0 through 18 or (resid 19...DD0 - 181 - 19
222HISHISHISHIS(chain D and (resid 0 through 18 or (resid 19...DD1920
232METMETGLUGLU(chain D and (resid 0 through 18 or (resid 19...DD0 - 3721 - 373
242METMETGLUGLU(chain D and (resid 0 through 18 or (resid 19...DD0 - 3721 - 373
252METMETGLUGLU(chain D and (resid 0 through 18 or (resid 19...DD0 - 3721 - 373
262METMETGLUGLU(chain D and (resid 0 through 18 or (resid 19...DD0 - 3721 - 373

NCS ensembles :
ID
1
2

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Components

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Protein , 2 types, 4 molecules ACBD

#1: Protein DNA-dependent ATPase XPBII


Mass: 50728.812 Da / Num. of mol.: 2 / Mutation: M1G
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (archaea)
Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7 / Gene: xpb2, ST1613, STK_16130 / Plasmid: pET-28a / Production host: Escherichia coli (E. coli) / Variant (production host): Rosetta(DE3)pLys-S / References: UniProt: Q970I2
#2: Protein Endonuclease Bax1


Mass: 44321.129 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (archaea)
Strain: DSM 16993 / JCM 10545 / NBRC 100140 / 7 / Gene: bax1, ST1614, STK_16140 / Plasmid: pET-15b / Production host: Escherichia coli (E. coli) / Variant (production host): Rosetta(DE3)pLys-S / References: UniProt: Q970I1

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DNA chain , 2 types, 4 molecules EGFH

#3: DNA chain DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*AP*AP*CP*AP*TP*CP*CP*TP*TP*TP*GP*CP*TP*AP*CP*AP*A)-3')


Mass: 7263.716 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#4: DNA chain DNA (5'-D(P*TP*GP*TP*AP*GP*GP*TP*TP*TP*CP*CP*AP*TP*GP*TP*TP*GP*AP*GP*TP*CP*A)-3')


Mass: 7092.579 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: TTGTAGGTTTCCATGTTGAGTCA / Source: (synth.) synthetic construct (others)

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Non-polymers , 3 types, 39 molecules

#5: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 25 / Source method: obtained synthetically / Formula: Mg
#6: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 11 / Source method: obtained synthetically / Formula: Cl
#7: Chemical ChemComp-PO4 / PHOSPHATE ION


Mass: 94.971 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: PO4

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.91 Å3/Da / Density % sol: 57.74 % / Description: 40 microns plate-like crystal
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.3 / Details: 50mM MES, pH 5.3; 10mM MgCl2; 26% MPD / PH range: 5.30-5.80 / Temp details: constant

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Data collection

DiffractionMean temperature: 100 K / Ambient temp details: constant / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 1.7883 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Apr 3, 2019 / Details: Bent cylinders, Stripes of Pt, Rh and clear
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.7883 Å / Relative weight: 1
ReflectionResolution: 3.55→127.432 Å / Num. all: 28493 / Num. obs: 28493 / % possible obs: 94.1 % / Redundancy: 3 % / Rpim(I) all: 0.148 / Rrim(I) all: 0.266 / Rsym value: 0.22 / Net I/av σ(I): 2.8 / Net I/σ(I): 4.9 / Num. measured all: 85678
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured allNum. unique obsRpim(I) allRrim(I) allRsym valueNet I/σ(I) obs% possible all
3.55-3.7431.4510.41251241050.9731.7551.4511.394.3
3.74-3.973.10.9920.71227040150.661.1970.9921.896.1
3.97-4.2430.5351.21119237540.360.6480.5352.895.4
4.24-4.582.80.2882.3910632330.2010.3530.2884.289.1
4.58-5.023.10.2143.21014232670.1420.2580.2145.597.2
5.02-5.6130.1923.6898029470.1290.2330.1925.896.6
5.61-6.482.80.1684.1712225010.1160.2060.1686.292
6.48-7.943.10.1096.3650720910.0730.1320.1098.792.6
7.94-11.2330.0599.8521717130.0390.0710.05912.595.8
11.23-39.73530.059.426308670.0340.0610.0514.185.8

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation3.55 Å39.74 Å
Translation3.55 Å39.74 Å

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Processing

Software
NameVersionClassification
MOSFLM7.2.2data reduction
SCALA3.3.22data scaling
PHASER2.8.3phasing
PHENIX1.15.2-3472refinement
PDB_EXTRACT3.25data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5TNU and 6P4W

5tnu

6p4w


Resolution: 3.55→39.735 Å / SU ML: 0.66 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 31.94
RfactorNum. reflection% reflection
Rfree0.2716 1389 4.9 %
Rwork0.2502 --
obs0.2513 28335 93.47 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 306.89 Å2 / Biso mean: 108.8579 Å2 / Biso min: 31.38 Å2
Refinement stepCycle: final / Resolution: 3.55→39.735 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12309 1848 51 0 14208
Biso mean--76.73 --
Num. residues----1708
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A2540X-RAY DIFFRACTION5.653TORSIONAL
12C2540X-RAY DIFFRACTION5.653TORSIONAL
21B2090X-RAY DIFFRACTION5.653TORSIONAL
22D2090X-RAY DIFFRACTION5.653TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3.5501-3.67690.48091330.40892509264288
3.6769-3.8240.3641340.36992739287396
3.824-3.99790.35891410.33772774291596
3.9979-4.20840.31261430.3032712285596
4.2084-4.47180.27321290.26472696282593
4.4718-4.81650.2681350.23472586272190
4.8165-5.30020.26741490.23142803295297
5.3002-6.06480.2861470.24712763291096
6.0648-7.63210.29631340.2392597273189
7.6321-39.73760.16021440.16922767291193
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.0170.0673-0.02930.06360.00570.0661-0.20690.30730.2638-0.18540.16040.05730.352-0.1031-0.00210.7799-0.064-0.03921.1257-0.07920.665222.585711.1998-16.4857
20.04860.048-0.01180.0779-0.04170.1922-0.08740.2107-0.0123-0.15840.1203-0.2159-0.073-0.404300.53450.0483-0.05320.8869-0.05140.483923.099723.49310.305
30.07240.1304-0.17570.0771-0.16710.1089-0.081-0.0399-0.3123-0.64640.421-0.1803-0.30510.39160.0928-0.01460.413-0.06240.3934-0.28491.242957.484527.080716.6133
40.0833-0.02010.02720.15980.07480.1757-0.18570.11560.05460.32620.17380.0878-0.0861-0.024300.3760.0960.09530.37990.04560.597235.073635.321823.3905
50.0094-0.19340.139-0.05350.1922-0.06750.14790.4264-0.7190.96460.0126-0.47250.05650.17620.0148-0.05010.6902-0.565-0.61810.7688-0.425543.85659.093530.5518
60.30220.03380.12080.21140.20330.53-0.18130.00910.15220.05440.00270.24630.1056-0.1663-0.41640.5473-0.01030.03640.5486-0.07730.805927.7621-19.05128.2804
70.60990.3087-0.13770.2064-0.27210.47630.23810.54050.07890.0646-0.17490.14380.0119-0.14650.50910.4530.0332-0.07990.7684-0.12130.5213-11.7392-19.703732.5623
80.3864-0.2420.1984-0.0146-0.10440.2496-0.2987-0.1205-0.1460.30550.05270.13160.25410.07-0.29850.70010.0949-0.00210.4208-0.02620.475213.7946-33.411258.4184
90.0758-0.0044-0.04520.05940.12090.0552-0.58050.384-0.44550.1135-0.2256-0.70780.359-0.3612-0.08170.21260.22710.436-0.4003-0.68-0.240415.7039-34.77241.5298
100.0409-0.08490.0196-0.0269-0.04260.104-0.2580.0065-0.10450.37250.2694-0.0397-0.24230.042600.6428-0.0661-0.190.4239-0.11720.519920.7129-4.905359.6951
110.11590.00220.0375-0.00590.0777-0.0079-0.1096-0.13670.03080.1706-0.1286-0.0880.14070.2857-0.00010.7053-0.0409-0.01320.8703-0.08470.906635.4775-24.265141.2803
120.1086-0.0836-0.01520.0622-0.02140.07660.1552-0.00980.1347-0.1356-0.0588-0.2347-0.0472-0.027700.8583-0.02790.13620.3286-0.10170.54357.212112.088149.4415
130.10260.041-0.090.14850.10360.13320.44020.01750.1725-0.1007-0.0781-0.3382-0.32450.00260.19471.02570.14010.06690.64160.09170.73660.600524.172231.071
140.01380.0037-0.0006-0.0092-0.00460.01130.25110.1843-0.029-0.1936-0.13830.04860.1217-0.1405-01.26090.2550.08811.1160.09470.5499-5.240719.538623.5081
150.0038-0.0005-0.00310.0052-0.00470.0066-0.123-0.04380.0383-0.027-0.03710.09010.04080.04940.00011.4819-0.0926-0.12441.8196-0.09631.728623.35138.334926.86
16-0.01240.04170.0255-0.0103-0.0361-0.0445-0.2936-0.15180.2568-0.12460.0466-0.07080.2370.1782-0.00040.7285-0.0243-0.02180.7394-0.0570.862242.834317.739311.9741
170.02450.0343-0.00560.01390.0145-0.0072-0.0669-0.0097-0.18-0.00170.1014-0.20050.12020.019301.9431-0.04850.45241.83360.17382.498452.781451.52747.6716
180.0066-0.00560.00830.0037-0.0027-0.0059-0.03670.03760.1156-0.0568-0.19070.0469-0.1017-0.04690.00011.6827-0.26970.44081.8927-0.21.752357.518211.8262-14.5928
190.01040.00240.01190.00580.00920.005-0.1429-0.06170.06540.0065-0.0790.0848-0.0545-0.06710.00021.0047-0.0234-0.15761.1841-0.10951.157552.19085.29762.6198
200.0173-0.04420.0456-0.0003-0.0265-0.01810.09280.0366-0.09010.1843-0.35710.11010.1631-0.0361-00.6872-0.0606-0.00770.68660.00970.710134.9611.058918.6328
210.00210.0028-0.00830.00640.00050.00260.114-0.0271-0.0529-0.155-0.01330.04190.01140.0597-01.90310.01960.2212.09950.12462.060419.7199-9.632430.6887
22-0.0030.0054-0.00250.00050.02230.0064-0.07770.0739-0.11960.110.00870.05190.06410.1843-00.7258-0.11960.05090.8956-0.10490.90798.775-10.656945.6237
230.01260.00890.0133-0.0073-0.00150.0040.17150.2150.09330.1360.124-0.13130.00920.0238-0.00040.9127-0.11280.19820.9233-0.04550.54844.0354-26.996356.9743
24-0.02450.0124-0.03730.00610.01080.01480.47260.1287-0.20390.10820.36310.0234-0.090.2165-0.00021.63810.02510.66031.45-0.07062.19015.0196-54.208464.8621
25-0.00430.01150.00970.0017-0.00220.008-0.0027-0.04680.02130.0002-0.0580.06330.01590.058701.27380.1753-0.17191.20110.08530.8947-13.3466-6.122668.4298
260.0093-0.0053-0.00190.00190.0004-0.0029-0.1907-0.054-0.0758-0.0072-0.15030.0503-0.06950.0984-0.00011.3827-0.26090.1310.96090.02831.23595.8358-11.945358.9108
270.01320.00880.0027-0.01660.00060.0075-0.0776-0.067-0.10490.0306-0.20430.1293-0.1332-0.0060.00020.6738-0.05590.01930.82630.08250.733311.9456-19.288244.8417
280.0056-0.01270.0060.00920.0039-0.00290.1889-0.1176-0.0179-0.05450.1545-0.07230.14260.0636-0.00031.1113-0.2589-0.04781.11290.07431.000513.4958-3.223834.6022
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 0 through 70 )A0 - 70
2X-RAY DIFFRACTION2chain 'A' and (resid 71 through 233 )A71 - 233
3X-RAY DIFFRACTION3chain 'A' and (resid 234 through 306 )A234 - 306
4X-RAY DIFFRACTION4chain 'A' and (resid 307 through 434 )A307 - 434
5X-RAY DIFFRACTION5chain 'B' and (resid 0 through 163 )B0 - 163
6X-RAY DIFFRACTION6chain 'B' and (resid 164 through 372 )B164 - 372
7X-RAY DIFFRACTION7chain 'C' and (resid 0 through 223 )C0 - 223
8X-RAY DIFFRACTION8chain 'C' and (resid 224 through 333 )C224 - 333
9X-RAY DIFFRACTION9chain 'C' and (resid 334 through 435 )C334 - 435
10X-RAY DIFFRACTION10chain 'D' and (resid 0 through 71 )D0 - 71
11X-RAY DIFFRACTION11chain 'D' and (resid 72 through 137 )D72 - 137
12X-RAY DIFFRACTION12chain 'D' and (resid 138 through 253 )D138 - 253
13X-RAY DIFFRACTION13chain 'D' and (resid 254 through 329 )D254 - 329
14X-RAY DIFFRACTION14chain 'D' and (resid 330 through 372 )D330 - 372
15X-RAY DIFFRACTION15chain 'E' and (resid 1 through 5 )E1 - 5
16X-RAY DIFFRACTION16chain 'E' and (resid 6 through 15 )E6 - 15
17X-RAY DIFFRACTION17chain 'E' and (resid 16 through 23 )E16 - 23
18X-RAY DIFFRACTION18chain 'F' and (resid 3 through 7 )F3 - 7
19X-RAY DIFFRACTION19chain 'F' and (resid 8 through 12 )F8 - 12
20X-RAY DIFFRACTION20chain 'F' and (resid 13 through 24 )F13 - 24
21X-RAY DIFFRACTION21chain 'G' and (resid 1 through 5 )G1 - 5
22X-RAY DIFFRACTION22chain 'G' and (resid 6 through 10 )G6 - 10
23X-RAY DIFFRACTION23chain 'G' and (resid 11 through 15 )G11 - 15
24X-RAY DIFFRACTION24chain 'G' and (resid 16 through 24 )G16 - 24
25X-RAY DIFFRACTION25chain 'H' and (resid 5 through 9 )H5 - 9
26X-RAY DIFFRACTION26chain 'H' and (resid 10 through 14 )H10 - 14
27X-RAY DIFFRACTION27chain 'H' and (resid 15 through 19 )H15 - 19
28X-RAY DIFFRACTION28chain 'H' and (resid 20 through 24 )H20 - 24

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