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- PDB-6p1a: Transcription antitermination factor Q21 in complex with Q21-bind... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6p1a | ||||||
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Title | Transcription antitermination factor Q21 in complex with Q21-binding-element DNA | ||||||
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![]() | GENE REGULATION / RNA polymerase / DNA Binding / transcription / Q-dependent antitermination / Q antitermination factor | ||||||
Function / homology | Bacteriophage 933W, GpQ / Phage antitermination protein Q / negative regulation of termination of DNA-templated transcription / DNA binding / DNA / DNA (> 10) / Q protein![]() | ||||||
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Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Yin, Z. / Ebright, R.H. | ||||||
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![]() | ![]() Title: Structural basis of Q-dependent antitermination. Authors: Zhou Yin / Jason T Kaelber / Richard H Ebright / ![]() Abstract: Lambdoid bacteriophage Q protein mediates the switch from middle to late bacteriophage gene expression by enabling RNA polymerase (RNAP) to read through transcription terminators preceding ...Lambdoid bacteriophage Q protein mediates the switch from middle to late bacteriophage gene expression by enabling RNA polymerase (RNAP) to read through transcription terminators preceding bacteriophage late genes. Q loads onto RNAP engaged in promoter-proximal pausing at a Q binding element (QBE) and adjacent sigma-dependent pause element (SDPE) to yield a Q-loading complex, and Q subsequently translocates with RNAP as a pausing-deficient, termination-deficient Q-loaded complex. Here, we report high-resolution structures of 4 states on the pathway of antitermination by Q from bacteriophage 21 (Q21): Q21, the Q21-QBE complex, the Q21-loading complex, and the Q21-loaded complex. The results show that Q21 forms a torus, a "nozzle," that narrows and extends the RNAP RNA-exit channel, extruding topologically linked single-stranded RNA and preventing the formation of pause and terminator hairpins. | ||||||
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Data in XML | ![]() | 18.9 KB | Display | |
Data in CIF | ![]() | 25.6 KB | Display | |
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-Related structure data
Related structure data | ![]() 6p18C ![]() 6p19C ![]() 6p1bSC ![]() 6p1cC S: Starting model for refinement C: citing same article ( |
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Assembly
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Noncrystallographic symmetry (NCS) | NCS domain:
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