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Open data
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Basic information
Entry | Database: PDB / ID: 4b3n | |||||||||
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Title | Crystal structure of rhesus TRIM5alpha PRY/SPRY domain | |||||||||
![]() | MALTOSE-BINDING PERIPLASMIC PROTEIN, TRIPARTITE MOTIF-CONTAINING PROTEIN 5 | |||||||||
![]() | SUGAR BINDING PROTEIN/LIGASE / SUGAR BINDING PROTEIN-LIGASE COMPLEX / SUGAR BINDING PROTEIN-LIGASE CHIMERA | |||||||||
Function / homology | ![]() regulation of viral entry into host cell / regulation of lipopolysaccharide-mediated signaling pathway / suppression of viral release by host / negative regulation of viral entry into host cell / negative regulation of viral transcription / pattern recognition receptor activity / negative regulation of viral genome replication / detection of maltose stimulus / maltose transport complex / carbohydrate transport ...regulation of viral entry into host cell / regulation of lipopolysaccharide-mediated signaling pathway / suppression of viral release by host / negative regulation of viral entry into host cell / negative regulation of viral transcription / pattern recognition receptor activity / negative regulation of viral genome replication / detection of maltose stimulus / maltose transport complex / carbohydrate transport / protein K63-linked ubiquitination / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / positive regulation of autophagy / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / activation of innate immune response / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / P-body / RING-type E3 ubiquitin transferase / autophagy / positive regulation of DNA-binding transcription factor activity / protein polyubiquitination / ubiquitin-protein transferase activity / regulation of protein localization / ubiquitin protein ligase activity / positive regulation of NF-kappaB transcription factor activity / outer membrane-bounded periplasmic space / positive regulation of canonical NF-kappaB signal transduction / defense response to virus / positive regulation of MAPK cascade / periplasmic space / innate immune response / DNA damage response / protein kinase binding / protein homodimerization activity / zinc ion binding / nucleoplasm / identical protein binding / membrane / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Yang, H. / Ji, X. / Zhao, Q. / Xiong, Y. | |||||||||
![]() | ![]() Title: Structural Insight Into HIV-1 Capsid Recognition by Rhesus Trim5Alpha Authors: Yang, H. / Ji, X. / Zhao, G. / Ning, J. / Zhao, Q. / Aiken, C. / Gronenborn, A.M. / Zhang, P. / Xiong, Y. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 459.2 KB | Display | ![]() |
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PDB format | ![]() | 380.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 44.9 KB | Display | |
Data in CIF | ![]() | 60.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1anfS ![]() 2vol ![]() 4auf ![]() 4aug ![]() 4auh S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 67269.273 Da / Num. of mol.: 2 Fragment: MBP RESIDUES 27-395, TRIM5ALPHA PRY/SPRY DOMAIN RESIDUES 275-493 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() Plasmid: PMAT9S / Production host: ![]() ![]() #2: Polysaccharide | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 59 % / Description: NONE |
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Crystal grow | pH: 6.2 Details: 6% (W/V) GLUCOSE, 6% (W/V) TREHALOSE, 100 MM MES PH 6.2 AND 25% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Apr 30, 2012 Details: HORIZONTAL FOCUSING SAGITTAL BEND SECOND MONO CRYSTAL AND VERTICALLY FOCUSING MIRROR |
Radiation | Monochromator: CRYOGENICALLY COOLED DOUBLE CRYSTAL MONOCHROMETER Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.075 Å / Relative weight: 1 |
Reflection | Resolution: 3.3→50 Å / Num. obs: 22400 / % possible obs: 96.5 % / Observed criterion σ(I): 1 / Redundancy: 3 % / Rmerge(I) obs: 0.17 / Net I/σ(I): 6.69 |
Reflection shell | Resolution: 3.3→3.42 Å / Redundancy: 3 % / Rmerge(I) obs: 0.89 / Mean I/σ(I) obs: 1.39 / % possible all: 97.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRIES 1ANF, 2VOL Resolution: 3.3→49.42 Å / Cor.coef. Fo:Fc: 0.923 / Cor.coef. Fo:Fc free: 0.875 / SU B: 59.229 / SU ML: 0.429 / Cross valid method: THROUGHOUT / ESU R Free: 0.546 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 85.292 Å2
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Refinement step | Cycle: LAST / Resolution: 3.3→49.42 Å
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