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- PDB-1lls: CRYSTAL STRUCTURE OF UNLIGANDED MALTOSE BINDING PROTEIN WITH XENON -
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Open data
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Basic information
Entry | Database: PDB / ID: 1lls | ||||||
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Title | CRYSTAL STRUCTURE OF UNLIGANDED MALTOSE BINDING PROTEIN WITH XENON | ||||||
![]() | Maltose-binding periplasmic protein | ||||||
![]() | SUGAR BINDING PROTEIN / Hydrophobic Cavities / Ligand-Protein Interactions / Xenon Binding / Xenon Derivative | ||||||
Function / homology | ![]() detection of maltose stimulus / maltose transport complex / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / cell chemotaxis ...detection of maltose stimulus / maltose transport complex / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / outer membrane-bounded periplasmic space / periplasmic space / DNA damage response / membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Rubin, S.M. / Lee, S.-Y. / Ruiz, E.J. / Pines, A. / Wemmer, D.E. | ||||||
![]() | ![]() Title: DETECTION AND CHARACTERIZATION OF XENON-BINDING SITES IN PROTEINS BY 129XE NMR SPECTROSCOPY Authors: Rubin, S.M. / Lee, S.-Y. / Ruiz, E.J. / Pines, A. / Wemmer, D.E. #1: ![]() Title: Detection of a Conformational Change in Maltose Binding Protein by 129Xe Nuclear Magnetic Resonance Authors: Rubin, S.M. / Spence, M.M. / Dimitrov, I.E. / Ruiz, E.J. / Pines, A. / Wemmer, D.E. #2: ![]() Title: The 2.3 A Structure of the Malto- or Maltodextrin-Binding Protein, a Primary Receptor of Bacterial Active Transport Authors: Spurlino, J.C. / Lu, G.-Y. / Quiocho, F.A. #3: ![]() Title: Crystallographic Evidence of a Large Ligand-Induced Hinge-Twist Motion Between the Two Domains of the Maltodextrin Binding Protein Involved in Active Transport and Chemotaxis Authors: Sharff, A.J. / Rodseth, L.E. / Spurlino, J.C. / Quiocho, F.A. #4: ![]() Title: Size Versus Polarizability in Protein-Ligand Interactions: Binding of Noble Gases within Engineered Cavities in Phage T4 Lysozyme Authors: Quillin, M.L. / Breyer, W.A. / Griswold, I.J. / Matthews, B.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 87 KB | Display | ![]() |
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PDB format | ![]() | 64.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1ompS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 40753.152 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-XE / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.31 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.6 Details: 10 mM Sodium Citrate, 23% PEG 8000, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC / Detector: CCD / Date: Jan 31, 2002 |
Radiation | Monochromator: Double Crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→17.07 Å / Num. all: 55908 / Num. obs: 29855 / % possible obs: 92.3 % / Observed criterion σ(F): 4 / Observed criterion σ(I): 4 / Biso Wilson estimate: 16.9 Å2 / Rsym value: 0.029 / Net I/σ(I): 19.9 |
Reflection shell | Resolution: 1.8→1.91 Å / Mean I/σ(I) obs: 10.4 / Num. unique all: 4127 / Rsym value: 0.105 / % possible all: 76 |
Reflection | *PLUS Num. measured all: 55908 / Rmerge(I) obs: 0.029 |
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Processing
Software |
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Refinement | Method to determine structure: Isomorphous with Structure in Database Starting model: PDB ENTRY 1OMP Resolution: 1.8→17.07 Å / Rfactor Rfree error: 0.006 / Isotropic thermal model: OVERALL / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 41.8917 Å2 / ksol: 0.387004 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.5 Å2
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Refine analyze | Luzzati coordinate error free: 0.24 Å / Luzzati sigma a free: 0.15 Å | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→17.07 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.91 Å / Rfactor Rfree error: 0.018 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Num. reflection obs: 29755 / Rfactor all: 0.2051 / Rfactor obs: 0.204 / Rfactor Rfree: 0.226 / Rfactor Rwork: 0.204 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.258 / Rfactor Rwork: 0.241 |