MALTODEXTRINBINDINGPERIPLASMICPROTEIN / MALTODEXTRIN BINDING PROTEIN
Mass: 40741.098 Da / Num. of mol.: 1 / Mutation: I28T Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Plasmid: PBR322 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P02928, UniProt: P0AEX9*PLUS
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
4D 13C/15N-separated NOESY
1
2
1
4D 15N-seperated NOESY
1
3
1
3D 13C-separated NOESY
1
4
1
(HM)CMCB(CMHM)-NOESY
1
5
1
TROSY-based HNCO for dipolar couplings
2
6
2
TROSY-based HNCO for dipolar couplings
NMR details
Text: TROSY based HNCO sequences: J.B.NMR 14:333-343(1999)
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1.4 mM maltodextrin binding protein U-15N,13C,2H, with 1H amide and methyl on Val, Leu, and Ile(delta1) 2mM beta-cyclodextrin, 20 mM sodium phosphate pH 7.2, 3uM NaN3, 100uM EDTA.
90% H2O/10% D2O
2
1.4 mM maltodextrin binding protein U-15N,13C,2H, with 1H amide and methyl on Val, Leu, and Ile(delta1) 2mM beta-cyclodextrin, 20 mM sodium phosphate pH 7.2, 3uM NaN3, 100uM EDTA. ~19mg/ml pf1 bacteriophage
90% H2O/10% D2O
Sample conditions
Conditions-ID
pH
Pressure (kPa)
Temperature (K)
1
7.2
ambient
310K
2
7.2
ambient
310K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
600
1
Varian INOVA
Varian
INOVA
600
2
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Processing
NMR software
Name
Version
Classification
NMRPipe
1.7
processing
NMRView
3
dataanalysis
CNS
0.5
structuresolution
CNS
0.5
refinement
Refinement
Method: simulated annealing from extended coordinates torsion angle dynamics, finish with cartesian dynamics Software ordinal: 1
NMR ensemble
Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 243 / Conformers submitted total number: 10
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