1EZP

GLOBAL FOLD OF MALTODEXTRIN BINDING PROTEIN COMPLEXED WITH BETA-CYCLODEXTRIN USING PEPTIDE ORIENTATIONS FROM DIPOLAR COUPLINGS

Summary for 1EZP

• Entry DOI: 10.2210/pdb1ezp/pdb
• Related: 1EZO
• Descriptor: MALTODEXTRIN BINDING PERIPLASMIC PROTEIN (1 entity in total)
• Functional Keywords: residual diplar couplings, deuteration, methyl labeling, sugar binding protein
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 40741.10

Authors

Mueller, G.A.,Choy, W.Y.,Yang, D.,Forman-Kay, J.D.,Venters, R.A.,Kay, L.E. (deposition date: 2000-05-11, release date: 2001-05-08, Last modification date: 2024-05-22)

Primary citation

Mueller, G.A.,Choy, W.Y.,Yang, D.,Forman-Kay, J.D.,Venters, R.A.,Kay, L.E.
Global folds of proteins with low densities of NOEs using residual dipolar couplings: application to the 370-residue maltodextrin-binding protein.
J.Mol.Biol., 300:197-212, 2000

PubMed Abstract: The global fold of maltose-binding protein in complex with the substrate beta-cyclodextrin was determined by solution NMR methods. The two-domain protein is comprised of a single polypeptide chain of 370 residues, with a molecular mass of 42 kDa. Distance information in the form of H(N)-H(N), H(N)-CH(3) and CH(3)-CH(3) NOEs was recorded on (15)N, (2)H and (15)N, (13)C, (2)H-labeled proteins with methyl protonation in Val, Leu, and Ile (C(delta1) only) residues. Distances to methyl protons, critical for the structure determination, comprised 77 % of the long-range restraints. Initial structures were calculated on the basis of 1943 NOEs, 48 hydrogen bond and 555 dihedral angle restraints. A global pair-wise backbone rmsd of 5.5 A was obtained for these initial structures with rmsd values for the N and C domains of 2.4 and 3.8 A, respectively. Direct refinement against one-bond (1)H(N)-(15)N, (13)C(alpha)-(13)CO, (15)N-(13)CO, two-bond (1)H(N)-(13)CO and three-bond (1)H(N)-(13)C(alpha) dipolar couplings resulted in structures with large numbers of dipolar restraint violations. As an alternative to direct refinement against measured dipolar couplings we have developed an approach where discrete orientations are calculated for each peptide plane on the basis of the dipolar couplings described above. The orientation which best matches that in initial NMR structures calculated from NOE and dihedral angle restraints exclusively is used to refine further the structures using a new module written for CNS. Modeling studies from four different proteins with diverse structural motifs establishes the utility of the methodology. When applied to experimental data recorded on MBP the precision of the family of structures generated improves from 5.5 to 2.2 A, while the rmsd with respect to the X-ray structure (1dmb) is reduced from 5.1 to 3.3 A.

PubMed: 10864509
DOI: 10.1006/jmbi.2000.3842

Experimental method

SOLUTION NMR