Calicivirus coat protein C-terminal / Calicivirus coat protein C-terminal / Calicivirus coat protein / Calicivirus coat protein / Picornavirus/Calicivirus coat protein / Viral coat protein subunit / Viral protein 1
ジャーナル: Proc Natl Acad Sci U S A / 年: 2019 タイトル: High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations. 著者: James Jung / Timothy Grant / Dennis R Thomas / Chris W Diehnelt / Nikolaus Grigorieff / Leemor Joshua-Tor / 要旨: Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available ...Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available in detail for only a single strain (GI.1). We present high-resolution (2.6- to 4.1-Å) cryoelectron microscopy (cryo-EM) structures of GII.4, GII.2, GI.7, and GI.1 human norovirus outbreak strain virus-like particles (VLPs). Although norovirus VLPs have been thought to exist in a single-sized assembly, our structures reveal polymorphism between and within genogroups, with small, medium, and large particle sizes observed. Using asymmetric reconstruction, we were able to resolve a Zn metal ion adjacent to the coreceptor binding site, which affected the structural stability of the shell. Our structures serve as valuable templates for facilitating vaccine formulations.
平均露光時間: 7 sec. / 電子線照射量: 69 e/Å2 / 検出モード: SUPER-RESOLUTION フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 実像数: 2809
電子光学装置
エネルギーフィルター名称: GIF Quantum LS
画像スキャン
動画フレーム数/画像: 35
-
解析
EMソフトウェア
ID
名称
バージョン
カテゴリ
1
cisTEM
1.0.0
粒子像選択
2
EPU
1.1
画像取得
4
cisTEM
1.0.0
CTF補正
7
MOLREP
CCPEM
モデルフィッティング
8
Coot
0.8.9
モデルフィッティング
10
PHENIX
1.14
モデル精密化
11
cisTEM
1.0.0
初期オイラー角割当
12
cisTEM
1.0.0
最終オイラー角割当
13
cisTEM
1.0.0
分類
14
cisTEM
1.0.0
3次元再構成
CTF補正
タイプ: PHASE FLIPPING ONLY
粒子像の選択
選択した粒子像数: 76405
対称性
点対称性: C1 (非対称)
3次元再構成
解像度: 2.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 1486080 / アルゴリズム: FOURIER SPACE 詳細: Symmetry expansion and signal subtraction of the icosahedral asymmetric units from the whole particle images, followed by asymmetric focused reconstruction towards the apex of the spike domain 対称性のタイプ: POINT