[English] 日本語
![](img/lk-miru.gif)
- EMDB-20205: Asymmetric focused reconstruction of human norovirus GI.1 Norwalk... -
+
Open data
-
Basic information
Entry | Database: EMDB / ID: EMD-20205 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Asymmetric focused reconstruction of human norovirus GI.1 Norwalk strain VLP asymmetric unit in T=3 symmetry | |||||||||
![]() | Asymmetric focused reconstruction of human norovirus GI.1 Norwalk strain VLP asymmetric unit in T=3 symmetry | |||||||||
![]() |
| |||||||||
![]() | Caliciviridae / Norovirus / GI.1 / Norwalk / VIRUS LIKE PARTICLE | |||||||||
Function / homology | ![]() T=3 icosahedral viral capsid / host cell cytoplasm / identical protein binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.6 Å | |||||||||
![]() | Jung J / Grant T | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations. Authors: James Jung / Timothy Grant / Dennis R Thomas / Chris W Diehnelt / Nikolaus Grigorieff / Leemor Joshua-Tor / ![]() Abstract: Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available ...Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available in detail for only a single strain (GI.1). We present high-resolution (2.6- to 4.1-Å) cryoelectron microscopy (cryo-EM) structures of GII.4, GII.2, GI.7, and GI.1 human norovirus outbreak strain virus-like particles (VLPs). Although norovirus VLPs have been thought to exist in a single-sized assembly, our structures reveal polymorphism between and within genogroups, with small, medium, and large particle sizes observed. Using asymmetric reconstruction, we were able to resolve a Zn metal ion adjacent to the coreceptor binding site, which affected the structural stability of the shell. Our structures serve as valuable templates for facilitating vaccine formulations. | |||||||||
History |
|
-
Structure visualization
Movie |
![]() |
---|---|
Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
-
Downloads & links
-EMDB archive
Map data | ![]() | 1 MB | ![]() | |
---|---|---|---|---|
Header (meta data) | ![]() ![]() | 16.8 KB 16.8 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.8 KB | Display | ![]() |
Images | ![]() | 154.4 KB | ||
Filedesc metadata | ![]() | 6.4 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 503.4 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 503 KB | Display | |
Data in XML | ![]() | 8.7 KB | Display | |
Data in CIF | ![]() | 11.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6outMC ![]() 6otfC ![]() 6ou9C ![]() 6oucC ![]() 6ouuC C: citing same article ( M: atomic model generated by this map |
---|---|
Similar structure data |
-
Links
EMDB pages | ![]() ![]() |
---|---|
Related items in Molecule of the Month |
-
Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Asymmetric focused reconstruction of human norovirus GI.1 Norwalk strain VLP asymmetric unit in T=3 symmetry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-
Sample components
-Entire : Norovirus Hu/1968/US
Entire | Name: ![]() |
---|---|
Components |
|
-Supramolecule #1: Norovirus Hu/1968/US
Supramolecule | Name: Norovirus Hu/1968/US / type: virus / ID: 1 / Parent: 0 / Macromolecule list: #1 / NCBI-ID: 524364 / Sci species name: Norovirus Hu/1968/US / Sci species strain: GI.1 / Virus type: VIRUS-LIKE PARTICLE / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: Yes |
---|---|
Host (natural) | Organism: ![]() |
Molecular weight | Theoretical: 10.19 MDa |
Virus shell | Shell ID: 1 / Name: VP1 / Diameter: 410.0 Å / T number (triangulation number): 3 |
-Macromolecule #1: Capsid protein VP1
Macromolecule | Name: Capsid protein VP1 / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: ![]() Strain: GI/Human/United States/Norwalk/1968 |
Molecular weight | Theoretical: 56.629828 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MMMASKDATS SVDGASGAGQ LVPEVNASDP LAMDPVAGSS TAVATAGQVN PIDPWIINNF VQAPQGEFTI SPNNTPGDVL FDLSLGPHL NPFLLHLSQM YNGWVGNMRV RIMLAGNAFT AGKIIVSCIP PGFGSHNLTI AQATLFPHVI ADVRTLDPIE V PLEDVRNV ...String: MMMASKDATS SVDGASGAGQ LVPEVNASDP LAMDPVAGSS TAVATAGQVN PIDPWIINNF VQAPQGEFTI SPNNTPGDVL FDLSLGPHL NPFLLHLSQM YNGWVGNMRV RIMLAGNAFT AGKIIVSCIP PGFGSHNLTI AQATLFPHVI ADVRTLDPIE V PLEDVRNV LFHNNDRNQQ TMRLVCMLYT PLRTGGGTGD SFVVAGRVMT CPSPDFNFLF LVPPTVEQKT RPFTLPNLPL SS LSNSRAP LPISSMGISP DNVQSVQFQN GRCTLDGRLV GTTPVSLSHV AKIRGTSNGT VINLTELDGT PFHPFEGPAP IGF PDLGGC DWHINMTQFG HSSQTQYDVD TTPDTFVPHL GSIQANGIGS GNYVGVLSWI SPPSHPSGSQ VDLWKIPNYG SSIT EATHL APSVYPPGFG EVLVFFMSKM PGPGAYNLPC LLPQEYISHL ASEQAPTVGE AALLHYVDPD TGRNLGEFKA YPDGF LTCV PNGASSGPQQ LPINGVFVFV SWVSRFYQLK PVGTASSARG RLGLRR UniProtKB: Capsid protein VP1 |
-Macromolecule #2: water
Macromolecule | Name: water / type: ligand / ID: 2 / Number of copies: 226 / Formula: HOH |
---|---|
Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ![]() ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
![]() | single particle reconstruction |
Aggregation state | particle |
-
Sample preparation
Concentration | 4 mg/mL |
---|---|
Buffer | pH: 5.75 |
Grid | Material: COPPER / Support film - Material: CARBON / Support film - topology: LACEY / Pretreatment - Type: GLOW DISCHARGE / Details: unspecified |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 295 K / Instrument: LEICA EM GP |
-
Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Specialist optics | Energy filter - Name: GIF Quantum LS |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Number real images: 1820 / Average exposure time: 7.0 sec. / Average electron dose: 78.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 130000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
+
Image processing
-Atomic model buiding 1
Initial model |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
Refinement | Space: REAL / Protocol: AB INITIO MODEL | ||||||||
Output model | ![]() PDB-6out: |