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TitleHigh-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 116, Issue 26, Page 12828-12832, Year 2019
Publish dateJun 25, 2019
AuthorsJames Jung / Timothy Grant / Dennis R Thomas / Chris W Diehnelt / Nikolaus Grigorieff / Leemor Joshua-Tor /
PubMed AbstractNoroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available ...Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available in detail for only a single strain (GI.1). We present high-resolution (2.6- to 4.1-Å) cryoelectron microscopy (cryo-EM) structures of GII.4, GII.2, GI.7, and GI.1 human norovirus outbreak strain virus-like particles (VLPs). Although norovirus VLPs have been thought to exist in a single-sized assembly, our structures reveal polymorphism between and within genogroups, with small, medium, and large particle sizes observed. Using asymmetric reconstruction, we were able to resolve a Zn metal ion adjacent to the coreceptor binding site, which affected the structural stability of the shell. Our structures serve as valuable templates for facilitating vaccine formulations.
External linksProc Natl Acad Sci U S A / PubMed:31182604 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 4.1 Å
Structure data

20195

6otf

EMDB-20195, PDB-6otf:
Symmetric reconstruction of human norovirus GII.2 Snow Mountain Virus Strain VLP in T=3 symmetry
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-20197:
Symmetric reconstruction of human norovirus GI.7 Houston strain VLP in T=3 symmetry
Method: EM (single particle) / Resolution: 2.9 Å

20198

6ou9

EMDB-20198, PDB-6ou9:
Asymmetric focused reconstruction of human norovirus GI.7 Houston strain VLP asymmetric unit in T=3 symmetry
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-20199:
Symmetric reconstruction of human norovirus GI.1 Norwalk strain VLP in T=3 symmetry
Method: EM (single particle) / Resolution: 2.7 Å

EMDB-20201:
Symmetric reconstruction of human norovirus GII.2 Snow Mountain Virus Strain VLP in T=1 symmetry
Method: EM (single particle) / Resolution: 2.7 Å

20202

6ouc

EMDB-20202, PDB-6ouc:
Asymmetric focsued reconstruction of human norovirus GII.2 Snow Mountain Virus strain VLP asymmetric unit in T=1 symmetry
Method: EM (single particle) / Resolution: 2.8 Å

20205

6out

EMDB-20205, PDB-6out:
Asymmetric focused reconstruction of human norovirus GI.1 Norwalk strain VLP asymmetric unit in T=3 symmetry
Method: EM (single particle) / Resolution: 2.6 Å

20206

6ouu

EMDB-20206, PDB-6ouu:
Symmetric reconstruction of human norovirus GII.4 Minerva strain VLP in T=4 symmetry
Method: EM (single particle) / Resolution: 4.1 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • snow mountain virus
  • norovirus hu/gi.7/tch-060/usa/2003
  • Norovirus Hu/1968/US
  • norwalk virus (strain gi/human/united states/norwalk/1968)
  • norovirus hu/gii.4/minerva/2006/usa
KeywordsVIRUS LIKE PARTICLE / Caliciviridae / Norovirus / GII.2 / Snow Mountain Virus / Calicivirus / GI.7 / GI.1 / Norwalk / GII.4

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