EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	High-resolution cryo-EM structures of outbreak strain human norovirus shells reveal size variations.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 116, Issue 26, Page 12828-12832, Year 2019
掲載日	2019年6月25日
著者	James Jung / Timothy Grant / Dennis R Thomas / Chris W Diehnelt / Nikolaus Grigorieff / Leemor Joshua-Tor /
PubMed 要旨	Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available ...Noroviruses are a leading cause of foodborne illnesses worldwide. Although GII.4 strains have been responsible for most norovirus outbreaks, the assembled virus shell structures have been available in detail for only a single strain (GI.1). We present high-resolution (2.6- to 4.1-Å) cryoelectron microscopy (cryo-EM) structures of GII.4, GII.2, GI.7, and GI.1 human norovirus outbreak strain virus-like particles (VLPs). Although norovirus VLPs have been thought to exist in a single-sized assembly, our structures reveal polymorphism between and within genogroups, with small, medium, and large particle sizes observed. Using asymmetric reconstruction, we were able to resolve a Zn metal ion adjacent to the coreceptor binding site, which affected the structural stability of the shell. Our structures serve as valuable templates for facilitating vaccine formulations.
リンク	Proc Natl Acad Sci U S A / PubMed:31182604 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 4.1 Å
構造データ	20195 6otf EMDB-20195, PDB-6otf: Symmetric reconstruction of human norovirus GII.2 Snow Mountain Virus Strain VLP in T=3 symmetry 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-20197: Symmetric reconstruction of human norovirus GI.7 Houston strain VLP in T=3 symmetry 手法: EM (単粒子) / 解像度: 2.9 Å 20198 6ou9 EMDB-20198, PDB-6ou9: Asymmetric focused reconstruction of human norovirus GI.7 Houston strain VLP asymmetric unit in T=3 symmetry 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-20199: Symmetric reconstruction of human norovirus GI.1 Norwalk strain VLP in T=3 symmetry 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-20201: Symmetric reconstruction of human norovirus GII.2 Snow Mountain Virus Strain VLP in T=1 symmetry 手法: EM (単粒子) / 解像度: 2.7 Å 20202 6ouc EMDB-20202, PDB-6ouc: Asymmetric focsued reconstruction of human norovirus GII.2 Snow Mountain Virus strain VLP asymmetric unit in T=1 symmetry 手法: EM (単粒子) / 解像度: 2.8 Å 20205 6out EMDB-20205, PDB-6out: Asymmetric focused reconstruction of human norovirus GI.1 Norwalk strain VLP asymmetric unit in T=3 symmetry 手法: EM (単粒子) / 解像度: 2.6 Å 20206 6ouu EMDB-20206, PDB-6ouu: Symmetric reconstruction of human norovirus GII.4 Minerva strain VLP in T=4 symmetry 手法: EM (単粒子) / 解像度: 4.1 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-HOH: WATER / 水
由来	snow mountain virus (ウイルス) norovirus hu/gi.7/tch-060/usa/2003 (ノロウイルス) Norovirus Hu/1968/US (ノロウイルス) norwalk virus (strain gi/human/united states/norwalk/1968) (ノロウイルス) norovirus hu/gii.4/minerva/2006/usa (ノロウイルス)
キーワード	VIRUS LIKE PARTICLE (ウイルス様粒子) / Caliciviridae (カリシウイルス科) / Norovirus (ノロウイルス) / GII.2 / Snow Mountain Virus / Calicivirus (カリシウイルス科) / GI.7 / GI.1 / Norwalk / GII.4