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- PDB-6npr: Crystal structure of H-2Dd with C84-C139 disulfide in complex wit... -

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Basic information

Entry
Database: PDB / ID: 6npr
TitleCrystal structure of H-2Dd with C84-C139 disulfide in complex with gp120 derived peptide P18-I10
Components
  • ARG-GLY-PRO-GLY-ARG-ALA-PHE-VAL-THR-ILE
  • Beta-2-microglobulinBeta-2 microglobulin
  • H-2 class I histocompatibility antigen, D-D alpha chain
KeywordsIMMUNE SYSTEM / major histocompatibility complex / antigen presentation
Function / homology
Function and homology information


Synthesis and processing of ENV and VPU / evasion of host immune response / TAP1 binding / TAP2 binding / Alpha-defensins / positive regulation of antibody-dependent cellular cytotoxicity / regulation of natural killer cell mediated immunity / positive regulation of TRAIL production / antigen processing and presentation of exogenous peptide antigen via MHC class Ib / MHC class Ib protein complex ...Synthesis and processing of ENV and VPU / evasion of host immune response / TAP1 binding / TAP2 binding / Alpha-defensins / positive regulation of antibody-dependent cellular cytotoxicity / regulation of natural killer cell mediated immunity / positive regulation of TRAIL production / antigen processing and presentation of exogenous peptide antigen via MHC class Ib / MHC class Ib protein complex / positive regulation of natural killer cell mediated immunity / positive regulation of natural killer cell cytokine production / natural killer cell tolerance induction / natural killer cell lectin-like receptor binding / negative regulation of natural killer cell activation / cis-Golgi network membrane / positive regulation of natural killer cell activation / negative regulation of natural killer cell mediated cytotoxicity / positive regulation of interleukin-13 production / positive regulation of natural killer cell mediated cytotoxicity / Dectin-2 family / positive regulation of natural killer cell proliferation / T cell mediated cytotoxicity directed against tumor cell target / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent / positive regulation of memory T cell activation / positive regulation of immunoglobulin production / TAP complex binding / antigen processing and presentation of exogenous peptide antigen via MHC class I / Golgi medial cisterna / positive regulation of CD8-positive, alpha-beta T cell activation / CD8-positive, alpha-beta T cell activation / positive regulation of interleukin-4 production / positive regulation of CD8-positive, alpha-beta T cell proliferation / CD8 receptor binding / MHC class I protein binding / Binding and entry of HIV virion / endoplasmic reticulum exit site / beta-2-microglobulin binding / TAP binding / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / protection from natural killer cell mediated cytotoxicity / negative regulation of T cell proliferation / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / positive regulation of establishment of T cell polarity / virus-mediated perturbation of host defense response / detection of bacterium / T cell receptor binding / host cell endosome membrane / 14-3-3 protein binding / positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / early endosome lumen / positive regulation of receptor binding / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / actin filament organization / lumenal side of endoplasmic reticulum membrane / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide binding / Assembly Of The HIV Virion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / cellular response to iron(III) ion / negative regulation of forebrain neuron differentiation / Budding and maturation of HIV virion / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / response to molecule of bacterial origin / regulation of erythrocyte differentiation / regulation of iron ion transport / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / cellular response to iron ion / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / positive regulation of T cell mediated cytotoxicity / peptide antigen assembly with MHC class II protein complex / negative regulation of neurogenesis / MHC class II protein complex / positive regulation of receptor-mediated endocytosis / cellular response to nicotine / recycling endosome membrane / phagocytic vesicle membrane / specific granule lumen / peptide antigen binding / positive regulation of cellular senescence / antigen processing and presentation of exogenous peptide antigen via MHC class II / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / negative regulation of epithelial cell proliferation / Modulation by Mtb of host immune system / positive regulation of T cell activation / positive regulation of type II interferon production
Similarity search - Function
Envelope glycoprotein Gp160 / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I-like antigen recognition-like / Murine Class I Major Histocompatibility Complex, H2-DB; Chain A, domain 1 ...Envelope glycoprotein Gp160 / Retroviral envelope protein / Retroviral envelope protein GP41-like / Gp120 core superfamily / Envelope glycoprotein GP120 / Human immunodeficiency virus 1, envelope glycoprotein Gp120 / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I-like antigen recognition-like / Murine Class I Major Histocompatibility Complex, H2-DB; Chain A, domain 1 / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
H-2 class I histocompatibility antigen, D-D alpha chain / Envelope glycoprotein gp160 / Beta-2-microglobulin
Similarity search - Component
Biological speciesMus musculus (house mouse)
Homo sapiens (human)
Human immunodeficiency virus 1
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.37 Å
AuthorsToor, J. / McShan, A.C. / Tripathi, S.M. / Sgourakis, N.G.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)1R35GM125034-01 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)1R01AI143997 United States
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2019
Title: Molecular determinants of chaperone interactions on MHC-I for folding and antigen repertoire selection.
Authors: McShan, A.C. / Devlin, C.A. / Overall, S.A. / Park, J. / Toor, J.S. / Moschidi, D. / Flores-Solis, D. / Choi, H. / Tripathi, S. / Procko, E. / Sgourakis, N.G.
History
DepositionJan 18, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 22, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: H-2 class I histocompatibility antigen, D-D alpha chain
B: Beta-2-microglobulin
C: H-2 class I histocompatibility antigen, D-D alpha chain
D: Beta-2-microglobulin
P: ARG-GLY-PRO-GLY-ARG-ALA-PHE-VAL-THR-ILE
R: ARG-GLY-PRO-GLY-ARG-ALA-PHE-VAL-THR-ILE


Theoretical massNumber of molelcules
Total (without water)90,0916
Polymers90,0916
Non-polymers00
Water3,225179
1
A: H-2 class I histocompatibility antigen, D-D alpha chain
B: Beta-2-microglobulin
R: ARG-GLY-PRO-GLY-ARG-ALA-PHE-VAL-THR-ILE


Theoretical massNumber of molelcules
Total (without water)45,0453
Polymers45,0453
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4420 Å2
ΔGint-18 kcal/mol
Surface area19190 Å2
MethodPISA
2
C: H-2 class I histocompatibility antigen, D-D alpha chain
D: Beta-2-microglobulin
P: ARG-GLY-PRO-GLY-ARG-ALA-PHE-VAL-THR-ILE


Theoretical massNumber of molelcules
Total (without water)45,0453
Polymers45,0453
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4420 Å2
ΔGint-16 kcal/mol
Surface area19020 Å2
MethodPISA
Unit cell
Length a, b, c (Å)66.990, 105.330, 117.270
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number18
Space group name H-MP22121

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Components

#1: Protein H-2 class I histocompatibility antigen, D-D alpha chain / H-2D(D)


Mass: 32090.672 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: H2-D1 / Production host: Escherichia coli (E. coli) / References: UniProt: P01900
#2: Protein Beta-2-microglobulin / Beta-2 microglobulin


Mass: 11879.356 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Production host: Escherichia coli (E. coli) / References: UniProt: P61769
#3: Protein/peptide ARG-GLY-PRO-GLY-ARG-ALA-PHE-VAL-THR-ILE


Mass: 1075.265 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Human immunodeficiency virus 1 / References: UniProt: P04578*PLUS
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 179 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.36 Å3/Da / Density % sol: 47.94 %
Crystal growTemperature: 295 K / Method: vapor diffusion, sitting drop
Details: 0.2M Ammonium acetate, 0.1M Bis-Tris (5.5) and 25%PEG 3350

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Data collection

DiffractionMean temperature: 63 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Nov 9, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.37→78.362 Å / Num. obs: 34489 / % possible obs: 100 % / Redundancy: 12.6 % / CC1/2: 0.99 / Rmerge(I) obs: 0.13 / Net I/σ(I): 13.2
Reflection shellResolution: 2.37→2.46 Å / Redundancy: 11.8 % / Rmerge(I) obs: 0.66 / Mean I/σ(I) obs: 4 / Num. unique obs: 3576 / CC1/2: 0.88 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX(1.13_2998: ???)refinement
MOSFLMdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1QO3

1qo3


Resolution: 2.37→78.362 Å / SU ML: 0.28 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 23.82
RfactorNum. reflection% reflection
Rfree0.2509 1719 4.99 %
Rwork0.1781 --
obs0.1817 34437 99.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.37→78.362 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6303 0 0 179 6482
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0086483
X-RAY DIFFRACTIONf_angle_d0.9788797
X-RAY DIFFRACTIONf_dihedral_angle_d6.7873821
X-RAY DIFFRACTIONf_chiral_restr0.052890
X-RAY DIFFRACTIONf_plane_restr0.0061154
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3701-2.43980.30571310.20452699X-RAY DIFFRACTION100
2.4398-2.51860.31051280.2012687X-RAY DIFFRACTION100
2.5186-2.60860.29091400.2022690X-RAY DIFFRACTION100
2.6086-2.7130.29351700.20342666X-RAY DIFFRACTION100
2.713-2.83650.27271410.19932702X-RAY DIFFRACTION100
2.8365-2.98610.26221350.1912701X-RAY DIFFRACTION100
2.9861-3.17320.30431560.18862692X-RAY DIFFRACTION100
3.1732-3.41820.25251430.18142694X-RAY DIFFRACTION100
3.4182-3.76220.23441300.17032755X-RAY DIFFRACTION100
3.7622-4.30650.22741350.15062750X-RAY DIFFRACTION100
4.3065-5.42560.22731560.14842769X-RAY DIFFRACTION100
5.4256-78.40470.21171540.19592913X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.8755-0.9483-1.9513.6416-2.2145.6645-0.0792-0.0014-0.0003-0.0817-0.18570.0240.33130.0360.22680.1423-0.03420.03710.2302-0.00410.226413.98682.715425.4685
24.45850.47070.0941.4489-0.73532.46890.3264-0.3808-0.64670.22320.0317-0.40960.7019-0.3149-0.33730.5503-0.0507-0.10140.25950.08340.473410.7043-5.362533.4625
31.0938-0.1573-0.51642.0396-1.32214.9232-0.0244-0.0130.08490.03780.10740.1147-0.0964-0.1605-0.09340.12580.00860.0380.20790.00750.2389.743812.191523.0055
42.3328-0.78540.84082.435-2.33882.78380.237-0.12370.46370.22430.38820.372-0.5626-1.0526-0.68980.3280.00270.16890.32920.03510.38464.259321.54623.4508
52.6143-0.0210.85270.13150.05580.8853-0.0961-0.8622-0.0990.11490.10370.00340.1507-0.06160.05090.5312-0.08660.01710.29250.00610.32626.7223.903640.1584
65.31790.8407-1.50962.671-1.10761.2629-0.0884-0.04340.26140.0250.0607-0.0655-0.13420.06570.05490.275-0.0243-0.02880.279-0.00680.176147.09626.299830.6305
77.5218-1.6849-3.74715.90871.46088.6604-0.4142-0.97130.25820.63630.07170.11080.0420.36460.38910.5154-0.05460.02830.2796-0.0410.292646.41415.916441.8379
84.7756-6.3783.13469.6734-4.04664.00170.11040.36640.2948-0.0026-0.3201-0.46720.05410.00860.19150.2059-0.02240.01870.34870.01990.230530.59023.517616.4443
93.1109-1.41410.00374.0940.89660.3425-0.0392-0.544-0.88190.76050.2457-0.25721.25890.2834-0.28160.73450.1318-0.1740.380.01370.363140.9324-13.530632.145
103.56-0.23581.10460.91730.4092.59690.1367-0.197-0.37870.0573-0.04310.19040.33770.09270.02560.29230.0650.01330.3183-0.02660.203131.8321-5.272523.7482
113.833-0.65823.98124.4593-4.21917.45150.71280.665-0.5455-0.606-0.43340.530.28840.3668-0.33040.20990.00970.00510.2234-0.01340.196426.8297-5.859514.4452
125.4255-5.2837-5.9896.29445.09998.0148-0.51030.754-0.86380.1705-0.28030.91071.1019-0.02310.73140.5218-0.0591-0.00140.3937-0.12020.337929.2565-15.950517.9319
131.5127-0.44210.1864.0122-4.34294.7220.1401-0.1301-0.1363-0.1715-0.1047-0.41390.1536-0.0065-0.16220.2335-0.0492-0.00740.2268-0.00310.185621.62846.585422.6779
147.6695-4.58321.59492.9116-0.92073.3668-0.0983-0.054-0.30620.29290.2855-0.0480.5549-0.0876-0.23650.3284-0.0084-0.00510.1865-0.06830.283228.7157-7.663924.2763
153.26452.519-2.32056.6006-3.10253.4804-0.5695-0.5459-0.15670.3214-0.471-0.47260.67221.68770.46880.6570.3905-0.22760.842-0.23180.34844.4275-18.590621.1317
163.9493-2.12982.11161.0941-1.42535.06390.79840.8143-0.3028-1.0501-0.69910.01050.89440.4585-0.13760.41450.05770.01150.27450.00880.189531.3872-4.355910.0643
172.9774-2.55773.01675.4931-2.20793.08330.19830.09290.61120.2387-0.3663-1.4670.36521.40620.3380.3793-0.0187-0.05660.4706-0.030.491242.0903-6.790219.3263
182.26830.51370.45764.43870.11652.0174-0.15410.30420.2402-0.51250.24110.1241-0.15560.0525-0.0910.2355-0.06560.01630.26830.04360.212539.42130.1065-2.5717
192.78491.1427-1.25992.9598-4.23376.1342-0.03530.3408-0.1109-0.5308-0.1876-0.61560.19240.52130.24350.2218-0.01840.03140.3464-0.060.269148.877223.0639-0.0882
201.4809-0.02350.39892.242-1.15941.3594-0.1107-0.17260.08080.34120.0572-0.037-0.1716-0.05390.02480.19920.0113-0.00730.2036-0.02810.169940.680723.335413.9163
213.098-0.90080.54537.3948-3.06452.41780.29630.01350.06611.0348-0.6947-0.9262-0.39970.63660.32750.2773-0.0985-0.03260.31080.02230.21150.052121.809917.0894
223.15611.5768-1.74651.741-0.23121.3377-0.1180.51210.993-0.08270.1262-0.0564-0.09260.0261-0.08740.4383-0.03540.05210.1770.00610.535237.546845.37185.3683
236.9805-1.4682-1.8312.7781-0.19083.1087-0.3169-0.1129-0.10480.18110.01580.256-0.0498-0.10310.25910.25330.00290.00690.1407-0.01380.2347.847536.3788.7125
246.63422.99260.17742.6286-0.96981.556-0.4871-0.08870.48580.08260.18490.4441-0.35110.16010.24470.43170.0874-0.00220.2826-0.09310.4177.817745.333512.7635
259.0037-6.07574.84078.457-6.36718.18630.0290.343-0.62490.15770.08350.4075-0.40780.1263-0.01930.2069-0.03090.01680.22420.01690.115220.964620.61642.9544
260.04680.22260.18911.14871.34042.0197-0.56170.25480.68790.0321-0.4781-0.2563-0.5433-0.1086-0.05950.07880.3619-0.24790.23950.40130.416510.523539.9538-9.4952
273.8558-2.34370.53962.38250.00190.18660.08720.64680.51460.1028-0.444-0.1353-0.14380.25640.34810.1777-0.0176-0.0350.2780.04340.238519.514629.2313-4.3159
286.2828-3.43384.42334.3367-3.43957.60240.3330.7047-0.1123-0.6963-0.18250.05850.15840.0882-0.11690.252-0.02750.00610.3375-0.00510.210421.110322.2351-11.2276
295.8639-0.58570.58386.0359-4.67753.72030.02510.22530.60410.01540.32050.1689-0.4848-0.4725-0.19870.2333-0.02940.0140.17050.01210.247531.144925.1155.0972
308.1527-3.14186.05471.5444-2.6524.9668-0.1650.60070.03170.37440.1780.1344-0.6829-0.17220.05020.26690.03450.04270.36310.04890.212122.174129.534-7.1678
312.80421.89842.11332.12832.10422.1289-0.07421.1695-0.4577-0.44820.11041.1022-0.0224-1.2346-0.17210.3879-0.0007-0.08850.56060.020.52914.732130.3265-14.943
325.7744-2.17491.17253.8753-1.79573.4270.3960.4959-0.1543-0.4252-0.28560.47910.3257-0.4605-0.10040.2148-0.036-0.02810.2991-0.02290.191313.609920.6917-4.8006
332.64782.3481-2.04063.1238-3.47424.2450.0995-0.31090.016-0.7242-0.7063-1.3757-0.19290.11950.61490.4021-0.07590.0540.2753-0.0280.441150.5424.37376.6956
341.1728-0.97470.97665.8975-3.33322.94260.5811-0.9199-0.4271-0.36220.84050.81651.4126-2.5426-1.40780.5032-0.1787-0.07120.81460.18650.69592.666311.071424.7682
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 37 )
2X-RAY DIFFRACTION2chain 'A' and (resid 38 through 56 )
3X-RAY DIFFRACTION3chain 'A' and (resid 57 through 137 )
4X-RAY DIFFRACTION4chain 'A' and (resid 138 through 162 )
5X-RAY DIFFRACTION5chain 'A' and (resid 163 through 193 )
6X-RAY DIFFRACTION6chain 'A' and (resid 194 through 262 )
7X-RAY DIFFRACTION7chain 'A' and (resid 263 through 275 )
8X-RAY DIFFRACTION8chain 'B' and (resid 1 through 12 )
9X-RAY DIFFRACTION9chain 'B' and (resid 13 through 20 )
10X-RAY DIFFRACTION10chain 'B' and (resid 21 through 31 )
11X-RAY DIFFRACTION11chain 'B' and (resid 32 through 42 )
12X-RAY DIFFRACTION12chain 'B' and (resid 43 through 52 )
13X-RAY DIFFRACTION13chain 'B' and (resid 53 through 62 )
14X-RAY DIFFRACTION14chain 'B' and (resid 63 through 72 )
15X-RAY DIFFRACTION15chain 'B' and (resid 73 through 78 )
16X-RAY DIFFRACTION16chain 'B' and (resid 79 through 91 )
17X-RAY DIFFRACTION17chain 'B' and (resid 92 through 100 )
18X-RAY DIFFRACTION18chain 'C' and (resid 2 through 56 )
19X-RAY DIFFRACTION19chain 'C' and (resid 57 through 84 )
20X-RAY DIFFRACTION20chain 'C' and (resid 85 through 137 )
21X-RAY DIFFRACTION21chain 'C' and (resid 138 through 162 )
22X-RAY DIFFRACTION22chain 'C' and (resid 163 through 185 )
23X-RAY DIFFRACTION23chain 'C' and (resid 186 through 253 )
24X-RAY DIFFRACTION24chain 'C' and (resid 254 through 275 )
25X-RAY DIFFRACTION25chain 'D' and (resid 1 through 12 )
26X-RAY DIFFRACTION26chain 'D' and (resid 13 through 20 )
27X-RAY DIFFRACTION27chain 'D' and (resid 21 through 31 )
28X-RAY DIFFRACTION28chain 'D' and (resid 32 through 52 )
29X-RAY DIFFRACTION29chain 'D' and (resid 53 through 62 )
30X-RAY DIFFRACTION30chain 'D' and (resid 63 through 72 )
31X-RAY DIFFRACTION31chain 'D' and (resid 73 through 78 )
32X-RAY DIFFRACTION32chain 'D' and (resid 79 through 100 )
33X-RAY DIFFRACTION33chain 'P' and (resid 1 through 10 )
34X-RAY DIFFRACTION34chain 'R' and (resid 1 through 10 )

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