PDB-6nca: HLA-A2 (A*02:01) bound to a peptide from the Epstein-Barr virus B...

Basic information

• Entry: Database: PDB / ID: 6nca
• Title: HLA-A2 (A*02:01) bound to a peptide from the Epstein-Barr virus BRLF1 protein

Components

• Beta-2-microglobulin
• HLA class I histocompatibility antigen, A-2 alpha chain
• Replication and transcription activator

• Keywords: IMMUNE SYSTEM / EBV viral peptide / BRLF1 / HLA-A2

Function / homology

Function:

release from viral latency / viral tegument / positive regulation of memory T cell activation / T cell mediated cytotoxicity directed against tumor cell target / TAP complex binding / Golgi medial cisterna / positive regulation of CD8-positive, alpha-beta T cell activation / CD8-positive, alpha-beta T cell activation / positive regulation of CD8-positive, alpha-beta T cell proliferation / CD8 receptor binding / antigen processing and presentation of exogenous peptide antigen via MHC class I / beta-2-microglobulin binding / endoplasmic reticulum exit site / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent / TAP binding / protection from natural killer cell mediated cytotoxicity / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / detection of bacterium / T cell receptor binding / : / : / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / negative regulation of receptor binding / DAP12 interactions / cellular response to iron ion / lumenal side of endoplasmic reticulum membrane / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide antigen assembly with MHC class II protein complex / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / MHC class II protein complex / negative regulation of forebrain neuron differentiation / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of erythrocyte differentiation / regulation of iron ion transport / MHC class I peptide loading complex / response to molecule of bacterial origin / HFE-transferrin receptor complex / T cell mediated cytotoxicity / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / antigen processing and presentation of exogenous peptide antigen via MHC class II / MHC class I protein complex / positive regulation of immune response / peptide antigen binding / negative regulation of neurogenesis / positive regulation of T cell mediated cytotoxicity / positive regulation of receptor-mediated endocytosis / multicellular organismal-level iron ion homeostasis / positive regulation of T cell activation / cellular response to nicotine / positive regulation of type II interferon production / specific granule lumen / recycling endosome membrane / phagocytic vesicle membrane / positive regulation of cellular senescence / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / negative regulation of epithelial cell proliferation / Interferon gamma signaling / MHC class II protein complex binding / Interferon alpha/beta signaling / positive regulation of protein binding / antibacterial humoral response / Modulation by Mtb of host immune system / late endosome membrane / sensory perception of smell / tertiary granule lumen / DAP12 signaling / E3 ubiquitin ligases ubiquitinate target proteins / T cell receptor signaling pathway / negative regulation of neuron projection development / iron ion transport / T cell differentiation in thymus / ER-Phagosome pathway / protein refolding / early endosome membrane / protein homotetramerization / amyloid fibril formation / intracellular iron ion homeostasis / learning or memory / defense response to Gram-positive bacterium / immune response / Amyloid fiber formation / endoplasmic reticulum lumen / external side of plasma membrane / Golgi membrane / signaling receptor binding / lysosomal membrane / innate immune response / focal adhesion / Neutrophil degranulation / endoplasmic reticulum membrane / regulation of DNA-templated transcription / host cell nucleus / SARS-CoV-2 activates/modulates innate and adaptive immune responses

Domain/homology:

Herpesvirus transcription activation factor / Herpesvirus transcription activation factor (transactivator) / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I-like antigen recognition-like / Murine Class I Major Histocompatibility Complex, H2-DB; Chain A, domain 1 / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold / Immunoglobulins / Immunoglobulin-like / Sandwich / 2-Layer Sandwich / Mainly Beta / Alpha Beta

Component:

HLA class I histocompatibility antigen, A alpha chain / HLA class I histocompatibility antigen, A alpha chain / Beta-2-microglobulin / Replication and transcription activator

• Biological species: Homo sapiens (human); Epstein-Barr virus (Epstein-Barr virus)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.30000082539 Å
• Authors: Stern, L.J. / Selin, L.K. / Song, I.Y.

Funding support

United States, 5items

Organization	Grant number	Country
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)	AI-49320	United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)	AI-046629	United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)	AI-109858	United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)	AI038996	United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	T32-AI-007349-17	United States

• Citation: Journal: Plos Pathog. / Year: 2019; Title: CDR3 alpha drives selection of the immunodominant Epstein Barr virus (EBV) BRLF1-specific CD8 T cell receptor repertoire in primary infection.; Authors: Kamga, L. / Gil, A. / Song, I. / Brody, R. / Ghersi, D. / Aslan, N. / Stern, L.J. / Selin, L.K. / Luzuriaga, K.

History

Deposition	Dec 11, 2018	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Oct 23, 2019	Provider: repository / Type: Initial release
Revision 1.1	Dec 18, 2019	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.2	Jan 29, 2020	Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.3	Nov 6, 2024	Group: Advisory / Data collection / Database references / Structure summary Category: chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature / pdbx_unobs_or_zero_occ_atoms Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Assembly

Deposited unit

Y: Replication and transcription activator

u: Replication and transcription activator

z: Replication and transcription activator

y: Replication and transcription activator

8: Replication and transcription activator

w: Replication and transcription activator

4: Replication and transcription activator

3: Replication and transcription activator

W: Replication and transcription activator

6: Replication and transcription activator

x: Replication and transcription activator

5: Replication and transcription activator

X: Replication and transcription activator

v: Replication and transcription activator

2: Replication and transcription activator

U: Replication and transcription activator

Z: Replication and transcription activator

V: Replication and transcription activator

7: Replication and transcription activator

A: HLA class I histocompatibility antigen, A-2 alpha chain

a: Beta-2-microglobulin

B: HLA class I histocompatibility antigen, A-2 alpha chain

b: Beta-2-microglobulin

C: HLA class I histocompatibility antigen, A-2 alpha chain

c: Beta-2-microglobulin

D: HLA class I histocompatibility antigen, A-2 alpha chain

d: Beta-2-microglobulin

E: HLA class I histocompatibility antigen, A-2 alpha chain

F: HLA class I histocompatibility antigen, A-2 alpha chain

f: Beta-2-microglobulin

G: HLA class I histocompatibility antigen, A-2 alpha chain

g: Beta-2-microglobulin

H: HLA class I histocompatibility antigen, A-2 alpha chain

h: Beta-2-microglobulin

S: HLA class I histocompatibility antigen, A-2 alpha chain

s: Beta-2-microglobulin

J: HLA class I histocompatibility antigen, A-2 alpha chain

j: Beta-2-microglobulin

K: HLA class I histocompatibility antigen, A-2 alpha chain

k: Beta-2-microglobulin

L: HLA class I histocompatibility antigen, A-2 alpha chain

l: Beta-2-microglobulin

M: HLA class I histocompatibility antigen, A-2 alpha chain

m: Beta-2-microglobulin

N: HLA class I histocompatibility antigen, A-2 alpha chain

n: Beta-2-microglobulin

O: HLA class I histocompatibility antigen, A-2 alpha chain

o: Beta-2-microglobulin

P: HLA class I histocompatibility antigen, A-2 alpha chain

p: Beta-2-microglobulin

Q: HLA class I histocompatibility antigen, A-2 alpha chain

q: Beta-2-microglobulin

R: HLA class I histocompatibility antigen, A-2 alpha chain

r: Beta-2-microglobulin

I: HLA class I histocompatibility antigen, A-2 alpha chain

i: Beta-2-microglobulin

T: HLA class I histocompatibility antigen, A-2 alpha chain

t: Beta-2-microglobulin

1: Replication and transcription activator

e: Beta-2-microglobulin

Summary:

• 896 kDa, 60 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	896,097	60
Polymers	896,097	60
Non-polymers	0	0
Water	0	0

1

Y: Replication and transcription activator

I: HLA class I histocompatibility antigen, A-2 alpha chain

i: Beta-2-microglobulin

Summary:

• defined by author&software
• Evidence: gel filtration
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4390 Å2
ΔGint	-25 kcal/mol
Surface area	19250 Å2
Method	PISA

2

u: Replication and transcription activator

B: HLA class I histocompatibility antigen, A-2 alpha chain

b: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4340 Å2
ΔGint	-23 kcal/mol
Surface area	19110 Å2
Method	PISA

3

z: Replication and transcription activator

L: HLA class I histocompatibility antigen, A-2 alpha chain

l: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4450 Å2
ΔGint	-25 kcal/mol
Surface area	18880 Å2
Method	PISA

4

y: Replication and transcription activator

J: HLA class I histocompatibility antigen, A-2 alpha chain

j: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4390 Å2
ΔGint	-24 kcal/mol
Surface area	19020 Å2
Method	PISA

5

8: Replication and transcription activator

T: HLA class I histocompatibility antigen, A-2 alpha chain

t: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4360 Å2
ΔGint	-24 kcal/mol
Surface area	19010 Å2
Method	PISA

6

w: Replication and transcription activator

F: HLA class I histocompatibility antigen, A-2 alpha chain

f: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4350 Å2
ΔGint	-23 kcal/mol
Surface area	19130 Å2
Method	PISA

7

4: Replication and transcription activator

P: HLA class I histocompatibility antigen, A-2 alpha chain

p: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4430 Å2
ΔGint	-24 kcal/mol
Surface area	19000 Å2
Method	PISA

8

3: Replication and transcription activator

O: HLA class I histocompatibility antigen, A-2 alpha chain

o: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4440 Å2
ΔGint	-23 kcal/mol
Surface area	19140 Å2
Method	PISA

9

W: Replication and transcription activator

E: HLA class I histocompatibility antigen, A-2 alpha chain

e: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4470 Å2
ΔGint	-19 kcal/mol
Surface area	19040 Å2
Method	PISA

10

6: Replication and transcription activator

R: HLA class I histocompatibility antigen, A-2 alpha chain

r: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4400 Å2
ΔGint	-23 kcal/mol
Surface area	19020 Å2
Method	PISA

11

x: Replication and transcription activator

H: HLA class I histocompatibility antigen, A-2 alpha chain

h: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4480 Å2
ΔGint	-23 kcal/mol
Surface area	18890 Å2
Method	PISA

12

5: Replication and transcription activator

Q: HLA class I histocompatibility antigen, A-2 alpha chain

q: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4500 Å2
ΔGint	-24 kcal/mol
Surface area	18940 Å2
Method	PISA

13

X: Replication and transcription activator

G: HLA class I histocompatibility antigen, A-2 alpha chain

g: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4480 Å2
ΔGint	-25 kcal/mol
Surface area	19010 Å2
Method	PISA

14

v: Replication and transcription activator

D: HLA class I histocompatibility antigen, A-2 alpha chain

d: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4450 Å2
ΔGint	-22 kcal/mol
Surface area	18970 Å2
Method	PISA

15

2: Replication and transcription activator

N: HLA class I histocompatibility antigen, A-2 alpha chain

n: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4450 Å2
ΔGint	-21 kcal/mol
Surface area	19030 Å2
Method	PISA

16

U: Replication and transcription activator

A: HLA class I histocompatibility antigen, A-2 alpha chain

a: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4540 Å2
ΔGint	-23 kcal/mol
Surface area	19130 Å2
Method	PISA

17

Z: Replication and transcription activator

K: HLA class I histocompatibility antigen, A-2 alpha chain

k: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4500 Å2
ΔGint	-22 kcal/mol
Surface area	19150 Å2
Method	PISA

18

V: Replication and transcription activator

C: HLA class I histocompatibility antigen, A-2 alpha chain

c: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4480 Å2
ΔGint	-22 kcal/mol
Surface area	19100 Å2
Method	PISA

19

7: Replication and transcription activator

S: HLA class I histocompatibility antigen, A-2 alpha chain

s: Beta-2-microglobulin

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4430 Å2
ΔGint	-23 kcal/mol
Surface area	18940 Å2
Method	PISA

20

M: HLA class I histocompatibility antigen, A-2 alpha chain

m: Beta-2-microglobulin

1: Replication and transcription activator

Summary:

• defined by author&software
• 44.8 kDa, 3 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	44,805	3
Polymers	44,805	3
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4390 Å2
ΔGint	-23 kcal/mol
Surface area	19050 Å2
Method	PISA

Unit cell

Length a, b, c (Å)	189.910, 100.170, 292.410
Angle α, β, γ (deg.)	90.000, 94.430, 90.000
Int Tables number	4
Space group name H-M	P1211
Space group name Hall	P2yb
Symmetry operation	#1: x,y,z #2: -x,y+1/2,-z

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
1	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
2	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
3	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
4	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
5	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
6	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
7	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
8	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
9	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
10	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
11	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
12	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
13	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
14	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
15	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
16	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
17	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
18	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
19	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
20	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...
1	2	CHAIN 1
2	2	CHAIN 2
3	2	CHAIN 3
4	2	CHAIN 4
5	2	CHAIN 5
6	2	CHAIN 6
7	2	CHAIN 7
8	2	CHAIN 8
9	2	CHAIN U
10	2	CHAIN V
11	2	CHAIN W
12	2	CHAIN X
13	2	CHAIN Y
14	2	CHAIN Z
15	2	CHAIN U
16	2	CHAIN V
17	2	CHAIN W
18	2	CHAIN X
19	2	CHAIN Y
20	2	CHAIN Z
1	3	(CHAIN K AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
2	3	(CHAIN A AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
3	3	(CHAIN B AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
4	3	(CHAIN C AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
5	3	(CHAIN D AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
6	3	(CHAIN E AND (RESSEQ 2:10 OR RESSEQ 310:357 OR RESSEQ 359:374 OR RESSEQ 376: 399))
7	3	(CHAIN F AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
8	3	(CHAIN G AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
9	3	(CHAIN H AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
10	3	(CHAIN I AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
11	3	(CHAIN J AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
12	3	(CHAIN L AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
13	3	(CHAIN M AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
14	3	(CHAIN N AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
15	3	(CHAIN O AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
16	3	(CHAIN P AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
17	3	(CHAIN Q AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
18	3	(CHAIN R AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
19	3	(CHAIN S AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))
20	3	(CHAIN T AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))

NCS domain segments:

Dom-ID	Component-ID	Ens-ID	Selection details	Auth asym-ID	Auth seq-ID
1	1	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	1 - 16
1	2	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	18 - 57
1	3	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	59 - 81
1	4	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	83 - 86
1	5	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	88
1	6	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	90 - 110
1	7	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	112 - 115
1	8	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	129 - 175
1	9	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	177 - 225
1	10	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	227 - 241
1	11	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	2 - 241
1	12	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	243 - 254
1	13	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	257 - 261
1	14	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	263 - 267
1	15	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	269 - 272
1	16	1	(CHAIN T AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	T	274
2	1	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	1 - 16
2	2	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	18 - 57
2	3	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	59 - 81
2	4	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	83 - 86
2	5	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	88
2	6	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	90 - 110
2	7	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	112 - 115
2	8	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	129 - 175
2	9	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	177 - 225
2	10	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	227 - 241
2	11	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	2 - 241
2	12	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	243 - 254
2	13	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	257 - 261
2	14	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	263 - 267
2	15	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	269 - 272
2	16	1	(CHAIN A AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	A	274
3	1	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	1 - 16
3	2	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	18 - 57
3	3	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	59 - 81
3	4	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	83 - 86
3	5	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	88
3	6	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	90 - 110
3	7	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	112 - 115
3	8	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	129 - 175
3	9	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	177 - 225
3	10	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	227 - 241
3	11	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	2 - 241
3	12	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	243 - 254
3	13	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	257 - 261
3	14	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	263 - 267
3	15	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	269 - 272
3	16	1	(CHAIN B AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	B	274
4	1	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	1 - 16
4	2	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	18 - 57
4	3	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	59 - 81
4	4	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	83 - 86
4	5	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	88
4	6	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	90 - 110
4	7	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	112 - 115
4	8	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	129 - 175
4	9	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	177 - 225
4	10	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	227 - 241
4	11	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	2 - 241
4	12	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	243 - 254
4	13	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	257 - 261
4	14	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	263 - 267
4	15	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	269 - 272
4	16	1	(CHAIN C AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	C	274
5	1	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	1 - 16
5	2	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	18 - 57
5	3	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	59 - 81
5	4	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	83 - 86
5	5	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	88
5	6	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	90 - 110
5	7	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	112 - 115
5	8	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	129 - 175
5	9	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	177 - 225
5	10	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	227 - 241
5	11	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	2 - 241
5	12	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	243 - 254
5	13	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	257 - 261
5	14	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	263 - 267
5	15	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	269 - 272
5	16	1	(CHAIN D AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	D	274
6	1	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	1 - 16
6	2	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	18 - 57
6	3	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	59 - 81
6	4	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	83 - 86
6	5	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	88
6	6	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	90 - 110
6	7	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	112 - 115
6	8	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	129 - 175
6	9	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	177 - 225
6	10	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	227 - 241
6	11	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	2 - 241
6	12	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	243 - 254
6	13	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	257 - 261
6	14	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	263 - 267
6	15	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	269 - 272
6	16	1	(CHAIN E AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	E	274
7	1	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	1 - 16
7	2	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	18 - 57
7	3	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	59 - 81
7	4	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	83 - 86
7	5	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	88
7	6	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	90 - 110
7	7	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	112 - 115
7	8	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	129 - 175
7	9	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	177 - 225
7	10	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	227 - 241
7	11	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	2 - 241
7	12	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	243 - 254
7	13	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	257 - 261
7	14	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	263 - 267
7	15	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	269 - 272
7	16	1	(CHAIN F AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	F	274
8	1	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	1 - 16
8	2	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	18 - 57
8	3	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	59 - 81
8	4	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	83 - 86
8	5	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	88
8	6	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	90 - 110
8	7	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	112 - 115
8	8	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	129 - 175
8	9	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	177 - 225
8	10	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	227 - 241
8	11	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	2 - 241
8	12	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	243 - 254
8	13	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	257 - 261
8	14	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	263 - 267
8	15	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	269 - 272
8	16	1	(CHAIN G AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	G	274
9	1	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	1 - 16
9	2	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	18 - 57
9	3	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	59 - 81
9	4	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	83 - 86
9	5	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	88
9	6	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	90 - 110
9	7	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	112 - 115
9	8	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	129 - 175
9	9	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	177 - 225
9	10	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	227 - 241
9	11	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	2 - 241
9	12	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	243 - 254
9	13	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	257 - 261
9	14	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	263 - 267
9	15	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	269 - 272
9	16	1	(CHAIN H AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	H	274
10	1	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	1 - 16
10	2	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	18 - 57
10	3	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	59 - 81
10	4	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	83 - 86
10	5	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	88
10	6	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	90 - 110
10	7	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	112 - 115
10	8	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	129 - 175
10	9	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	177 - 225
10	10	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	227 - 241
10	11	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	2 - 241
10	12	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	243 - 254
10	13	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	257 - 261
10	14	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	263 - 267
10	15	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	269 - 272
10	16	1	(CHAIN I AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	I	274
11	1	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	1 - 16
11	2	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	18 - 57
11	3	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	59 - 81
11	4	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	83 - 86
11	5	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	88
11	6	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	90 - 110
11	7	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	112 - 115
11	8	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	129 - 175
11	9	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	177 - 225
11	10	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	227 - 241
11	11	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	2 - 241
11	12	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	243 - 254
11	13	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	257 - 261
11	14	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	263 - 267
11	15	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	269 - 272
11	16	1	(CHAIN J AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	J	274
12	1	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	1 - 16
12	2	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	18 - 57
12	3	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	59 - 81
12	4	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	83 - 86
12	5	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	88
12	6	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	90 - 110
12	7	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	112 - 115
12	8	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	129 - 175
12	9	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	177 - 225
12	10	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	227 - 241
12	11	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	2 - 241
12	12	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	243 - 254
12	13	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	257 - 261
12	14	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	263 - 267
12	15	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	269 - 272
12	16	1	(CHAIN K AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	K	274
13	1	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	1 - 16
13	2	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	18 - 57
13	3	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	59 - 81
13	4	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	83 - 86
13	5	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	88
13	6	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	90 - 110
13	7	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	112 - 115
13	8	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	129 - 175
13	9	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	177 - 225
13	10	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	227 - 241
13	11	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	2 - 241
13	12	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	243 - 254
13	13	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	257 - 261
13	14	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	263 - 267
13	15	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	269 - 272
13	16	1	(CHAIN L AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	L	274
14	1	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	1 - 16
14	2	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	18 - 57
14	3	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	59 - 81
14	4	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	83 - 86
14	5	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	88
14	6	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	90 - 110
14	7	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	112 - 115
14	8	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	129 - 175
14	9	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	177 - 225
14	10	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	227 - 241
14	11	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	2 - 241
14	12	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	243 - 254
14	13	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	257 - 261
14	14	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	263 - 267
14	15	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	269 - 272
14	16	1	(CHAIN M AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	M	274
15	1	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	1 - 16
15	2	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	18 - 57
15	3	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	59 - 81
15	4	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	83 - 86
15	5	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	88
15	6	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	90 - 110
15	7	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	112 - 115
15	8	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	129 - 175
15	9	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	177 - 225
15	10	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	227 - 241
15	11	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	2 - 241
15	12	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	243 - 254
15	13	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	257 - 261
15	14	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	263 - 267
15	15	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	269 - 272
15	16	1	(CHAIN N AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	N	274
16	1	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	1 - 16
16	2	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	18 - 57
16	3	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	59 - 81
16	4	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	83 - 86
16	5	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	88
16	6	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	90 - 110
16	7	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	112 - 115
16	8	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	129 - 175
16	9	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	177 - 225
16	10	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	227 - 241
16	11	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	2 - 241
16	12	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	243 - 254
16	13	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	257 - 261
16	14	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	263 - 267
16	15	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	269 - 272
16	16	1	(CHAIN O AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	O	274
17	1	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	1 - 16
17	2	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	18 - 57
17	3	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	59 - 81
17	4	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	83 - 86
17	5	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	88
17	6	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	90 - 110
17	7	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	112 - 115
17	8	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	129 - 175
17	9	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	177 - 225
17	10	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	227 - 241
17	11	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	2 - 241
17	12	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	243 - 254
17	13	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	257 - 261
17	14	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	263 - 267
17	15	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	269 - 272
17	16	1	(CHAIN P AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	P	274
18	1	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	1 - 16
18	2	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	18 - 57
18	3	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	59 - 81
18	4	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	83 - 86
18	5	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	88
18	6	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	90 - 110
18	7	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	112 - 115
18	8	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	129 - 175
18	9	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	177 - 225
18	10	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	227 - 241
18	11	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	2 - 241
18	12	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	243 - 254
18	13	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	257 - 261
18	14	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	263 - 267
18	15	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	269 - 272
18	16	1	(CHAIN Q AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	Q	274
19	1	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	1 - 16
19	2	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	18 - 57
19	3	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	59 - 81
19	4	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	83 - 86
19	5	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	88
19	6	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	90 - 110
19	7	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	112 - 115
19	8	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	129 - 175
19	9	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	177 - 225
19	10	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	227 - 241
19	11	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	2 - 241
19	12	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	243 - 254
19	13	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	257 - 261
19	14	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	263 - 267
19	15	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	269 - 272
19	16	1	(CHAIN R AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	R	274
20	1	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	1 - 16
20	2	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	18 - 57
20	3	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	59 - 81
20	4	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	83 - 86
20	5	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	88
20	6	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	90 - 110
20	7	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	112 - 115
20	8	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	129 - 175
20	9	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	177 - 225
20	10	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	227 - 241
20	11	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	2 - 241
20	12	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	243 - 254
20	13	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	257 - 261
20	14	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	263 - 267
20	15	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	269 - 272
20	16	1	(CHAIN S AND (RESSEQ 1:16 OR RESSEQ 18:57 OR RESSEQ...	S	274
1	1	2	CHAIN 1	1	1 - 9
2	1	2	CHAIN 2	2	1 - 9
3	1	2	CHAIN 3	3	1 - 9
4	1	2	CHAIN 4	4	1 - 9
5	1	2	CHAIN 5	5	1 - 9
6	1	2	CHAIN 6	6	1 - 9
7	1	2	CHAIN 7	7	1 - 9
8	1	2	CHAIN 8	8	1 - 9
9	1	2	CHAIN U	U	1 - 9
10	1	2	CHAIN V	V	1 - 9
11	1	2	CHAIN W	W	1 - 9
12	1	2	CHAIN X	X	1 - 9
13	1	2	CHAIN Y	Y	1 - 9
14	1	2	CHAIN Z	Z	1 - 9
15	1	2	CHAIN U	U	1 - 9
16	1	2	CHAIN V	V	1 - 9
17	1	2	CHAIN W	W	1 - 9
18	1	2	CHAIN X	X	1 - 9
19	1	2	CHAIN Y	Y	1 - 9
20	1	2	CHAIN Z	Z	1 - 9
1	1	3	(CHAIN K AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	K	2 - 58
1	2	3	(CHAIN K AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	K	60 - 75
1	3	3	(CHAIN K AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	K	77 - 100
2	1	3	(CHAIN A AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	A	2 - 58
2	2	3	(CHAIN A AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	A	60 - 75
2	3	3	(CHAIN A AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	A	77 - 100
3	1	3	(CHAIN B AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	B	2 - 58
3	2	3	(CHAIN B AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	B	60 - 75
3	3	3	(CHAIN B AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	B	77 - 100
4	1	3	(CHAIN C AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	C	2 - 58
4	2	3	(CHAIN C AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	C	60 - 75
4	3	3	(CHAIN C AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	C	77 - 100
5	1	3	(CHAIN D AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	D	2 - 58
5	2	3	(CHAIN D AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	D	60 - 75
5	3	3	(CHAIN D AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	D	77 - 100
6	1	3	(CHAIN E AND (RESSEQ 2:10 OR RESSEQ 310:357 OR RESSEQ 359:374 OR RESSEQ 376: 399))	E	2 - 10
6	2	3	(CHAIN E AND (RESSEQ 2:10 OR RESSEQ 310:357 OR RESSEQ 359:374 OR RESSEQ 376: 399))	E	310 - 357
6	3	3	(CHAIN E AND (RESSEQ 2:10 OR RESSEQ 310:357 OR RESSEQ 359:374 OR RESSEQ 376: 399))	E	359 - 374
6	4	3	(CHAIN E AND (RESSEQ 2:10 OR RESSEQ 310:357 OR RESSEQ 359:374 OR RESSEQ 376: 399))	E	376 - 399
7	1	3	(CHAIN F AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	F	2 - 58
7	2	3	(CHAIN F AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	F	60 - 75
7	3	3	(CHAIN F AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	F	77 - 100
8	1	3	(CHAIN G AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	G	2 - 58
8	2	3	(CHAIN G AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	G	60 - 75
8	3	3	(CHAIN G AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	G	77 - 100
9	1	3	(CHAIN H AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	H	2 - 58
9	2	3	(CHAIN H AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	H	60 - 75
9	3	3	(CHAIN H AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	H	77 - 100
10	1	3	(CHAIN I AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	I	2 - 58
10	2	3	(CHAIN I AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	I	60 - 75
10	3	3	(CHAIN I AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	I	77 - 100
11	1	3	(CHAIN J AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	J	2 - 58
11	2	3	(CHAIN J AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	J	60 - 75
11	3	3	(CHAIN J AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	J	77 - 100
12	1	3	(CHAIN L AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	L	2 - 58
12	2	3	(CHAIN L AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	L	60 - 75
12	3	3	(CHAIN L AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	L	77 - 100
13	1	3	(CHAIN M AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	M	2 - 58
13	2	3	(CHAIN M AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	M	60 - 75
13	3	3	(CHAIN M AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	M	77 - 100
14	1	3	(CHAIN N AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	N	2 - 58
14	2	3	(CHAIN N AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	N	60 - 75
14	3	3	(CHAIN N AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	N	77 - 100
15	1	3	(CHAIN O AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	O	2 - 58
15	2	3	(CHAIN O AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	O	60 - 75
15	3	3	(CHAIN O AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	O	77 - 100
16	1	3	(CHAIN P AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	P	2 - 58
16	2	3	(CHAIN P AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	P	60 - 75
16	3	3	(CHAIN P AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	P	77 - 100
17	1	3	(CHAIN Q AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	Q	2 - 58
17	2	3	(CHAIN Q AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	Q	60 - 75
17	3	3	(CHAIN Q AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	Q	77 - 100
18	1	3	(CHAIN R AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	R	2 - 58
18	2	3	(CHAIN R AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	R	60 - 75
18	3	3	(CHAIN R AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	R	77 - 100
19	1	3	(CHAIN S AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	S	2 - 58
19	2	3	(CHAIN S AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	S	60 - 75
19	3	3	(CHAIN S AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	S	77 - 100
20	1	3	(CHAIN T AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	T	2 - 58
20	2	3	(CHAIN T AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	T	60 - 75
20	3	3	(CHAIN T AND (RESSEQ 2:58 OR RESSEQ 60:75 OR RESSEQ 77:100))	T	77 - 100

NCS ensembles :

ID
1
2
3

Components

#1: Protein/peptide

Y u z y 8 w 4 3 W 6 x 5 X v 2 U Z V 7 1
Replication and transcription activator / Rta / Immediate-early protein Rta

Details:

Mass: 1071.289 Da / Num. of mol.: 20 / Source method: obtained synthetically / Source: (synth.) Epstein-Barr virus (Epstein-Barr virus) / References: UniProt: Q3KSS7

#2: Protein

A B C D E F G H S J K L M N O P Q R I T
HLA class I histocompatibility antigen, A-2 alpha chain / MHC class I antigen A*2

Details:

Mass: 31854.203 Da / Num. of mol.: 20
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-A, HLAA
Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)
References: UniProt: P01892, UniProt: P04439*PLUS

#3: Protein

a b c d f g h s j k l m n o p q r i t e
Beta-2-microglobulin

Details:

Mass: 11879.356 Da / Num. of mol.: 20
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P
Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)
References: UniProt: P61769

• Has protein modification: Y

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 3.09 ų/Da / Density % sol: 60.25 %
• Crystal grow: Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8.5 ; Details: 10.5% (w/v) PEG 4000, 35 mM Tris base/ HCl (pH 8.5), 70 mM Li2SO4

Data collection

• Diffraction: Mean temperature: 100 K / Serial crystal experiment: N
• Diffraction source: Source: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.9793 Å
• Detector: Type: RAYONIX MX225HE / Detector: CCD / Date: Aug 20, 2015
• Radiation: Monochromator: Kohzu HLD-4 Double Crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.9793 Å / Relative weight: 1
• Reflection: Resolution: 3→111.42 Å / Num. obs: 217116 / % possible obs: 98.7 % / Redundancy: 3.3 % / B_iso Wilson estimate: 56.5236257247 Ų / CC_1/2: 0.915 / Net I/σ(I): 2.5
• Reflection shell: Resolution: 3→3.05 Å / Redundancy: 3.3 % / Num. unique obs: 10543 / CC_1/2: 0.161 / % possible all: 97.3

Processing

Software

Name	Version	Classification
PHENIX	1.10.1_2155	refinement
iMOSFLM		data reduction
SCALA		data scaling
PHASER		phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.30000082539→111.418903363 Å / SU ML: 0.456840245857 / Cross valid method: FREE R-VALUE / σ(F): 1.33702972307 / Phase error: 32.5017081896

	Rfactor	Num. reflection	% reflection
Rfree	0.302699764926	1516	0.926650366748 %
Rwork	0.285279623088	-	-
obs	0.285442550216	163600	98.7743766226 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
• Displacement parameters: B_iso mean: 58.1760972504 Ų

Refinement step

Cycle: LAST / Resolution: 3.30000082539→111.418903363 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	63200	0	0	0	63200

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.00279631690703	65040
X-RAY DIFFRACTION	f_angle_d	0.778874071584	88300
X-RAY DIFFRACTION	f_chiral_restr	0.0459529733274	9020
X-RAY DIFFRACTION	f_plane_restr	0.00531984510056	11500
X-RAY DIFFRACTION	f_dihedral_angle_d	14.7963020514	38320

Refine LS restraints NCS

Ens-ID	Dom-ID	Auth asym-ID	Number	Refine-ID	Type	Rms dev position (Å)
1	1	T	0	X-RAY DIFFRACTION	POSITIONAL	0
1	2	A	0	X-RAY DIFFRACTION	POSITIONAL	0
1	3	B	0	X-RAY DIFFRACTION	POSITIONAL	0
1	4	C	0	X-RAY DIFFRACTION	POSITIONAL	0
1	5	D	0	X-RAY DIFFRACTION	POSITIONAL	0
1	6	E	0	X-RAY DIFFRACTION	POSITIONAL	0
1	7	F	0	X-RAY DIFFRACTION	POSITIONAL	0
1	8	G	0	X-RAY DIFFRACTION	POSITIONAL	0
1	9	H	0	X-RAY DIFFRACTION	POSITIONAL	0
1	10	I	0	X-RAY DIFFRACTION	POSITIONAL	0
1	11	J	0	X-RAY DIFFRACTION	POSITIONAL	0
1	12	K	0	X-RAY DIFFRACTION	POSITIONAL	0
1	13	L	0	X-RAY DIFFRACTION	POSITIONAL	0
1	14	M	0	X-RAY DIFFRACTION	POSITIONAL	0
1	15	N	0	X-RAY DIFFRACTION	POSITIONAL	0
1	16	O	0	X-RAY DIFFRACTION	POSITIONAL	0
1	17	P	0	X-RAY DIFFRACTION	POSITIONAL	0
1	18	Q	0	X-RAY DIFFRACTION	POSITIONAL	0
1	19	R	0	X-RAY DIFFRACTION	POSITIONAL	0
1	20	S	0	X-RAY DIFFRACTION	POSITIONAL	0
2	1	1	0	X-RAY DIFFRACTION	POSITIONAL	0
2	2	2	0	X-RAY DIFFRACTION	POSITIONAL	0
2	3	3	0	X-RAY DIFFRACTION	POSITIONAL	0
2	4	4	0	X-RAY DIFFRACTION	POSITIONAL	0
2	5	5	0	X-RAY DIFFRACTION	POSITIONAL	0
2	6	6	0	X-RAY DIFFRACTION	POSITIONAL	0
2	7	7	0	X-RAY DIFFRACTION	POSITIONAL	0
2	8	8	0	X-RAY DIFFRACTION	POSITIONAL	0
2	9	U	0	X-RAY DIFFRACTION	POSITIONAL	0
2	10	V	0	X-RAY DIFFRACTION	POSITIONAL	0
2	11	W	0	X-RAY DIFFRACTION	POSITIONAL	0
2	12	X	0	X-RAY DIFFRACTION	POSITIONAL	0
2	13	Y	0	X-RAY DIFFRACTION	POSITIONAL	0
2	14	Z	0	X-RAY DIFFRACTION	POSITIONAL	0
2	15	U	0	X-RAY DIFFRACTION	POSITIONAL	0
2	16	V	0	X-RAY DIFFRACTION	POSITIONAL	0
2	17	W	0	X-RAY DIFFRACTION	POSITIONAL	0
2	18	X	0	X-RAY DIFFRACTION	POSITIONAL	0
2	19	Y	0	X-RAY DIFFRACTION	POSITIONAL	0
2	20	Z	0	X-RAY DIFFRACTION	POSITIONAL	0
3	1	K	0	X-RAY DIFFRACTION	POSITIONAL	0
3	2	A	0	X-RAY DIFFRACTION	POSITIONAL	0
3	3	B	0	X-RAY DIFFRACTION	POSITIONAL	0
3	4	C	0	X-RAY DIFFRACTION	POSITIONAL	0
3	5	D	0	X-RAY DIFFRACTION	POSITIONAL	0
3	6	E	0	X-RAY DIFFRACTION	POSITIONAL	0
3	7	F	0	X-RAY DIFFRACTION	POSITIONAL	0
3	8	G	0	X-RAY DIFFRACTION	POSITIONAL	0
3	9	H	0	X-RAY DIFFRACTION	POSITIONAL	0
3	10	I	0	X-RAY DIFFRACTION	POSITIONAL	0
3	11	J	0	X-RAY DIFFRACTION	POSITIONAL	0
3	12	L	0	X-RAY DIFFRACTION	POSITIONAL	0
3	13	M	0	X-RAY DIFFRACTION	POSITIONAL	0
3	14	N	0	X-RAY DIFFRACTION	POSITIONAL	0
3	15	O	0	X-RAY DIFFRACTION	POSITIONAL	0
3	16	P	0	X-RAY DIFFRACTION	POSITIONAL	0
3	17	Q	0	X-RAY DIFFRACTION	POSITIONAL	0
3	18	R	0	X-RAY DIFFRACTION	POSITIONAL	0
3	19	S	0	X-RAY DIFFRACTION	POSITIONAL	0
3	20	T	0	X-RAY DIFFRACTION	POSITIONAL	0

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
3.3-3.4065	0.336242375614	144	0.339366464636	14536	X-RAY DIFFRACTION	97.9777080691
3.4065-3.5283	0.40375942358	134	0.344939515024	14547	X-RAY DIFFRACTION	98.2729767722
3.5283-3.6696	0.351853089376	140	0.331043844656	14582	X-RAY DIFFRACTION	98.4946812069
3.6696-3.8366	0.345663086763	132	0.314964163063	14624	X-RAY DIFFRACTION	98.4718051385
3.8366-4.0389	0.332990354301	129	0.310353055621	14650	X-RAY DIFFRACTION	98.8826441857
4.0389-4.2919	0.307947979372	147	0.295751280802	14765	X-RAY DIFFRACTION	99.4199613308
4.2919-4.6233	0.309737179658	134	0.275477030866	14813	X-RAY DIFFRACTION	99.388257198
4.6233-5.0886	0.280362739466	134	0.260136844289	14777	X-RAY DIFFRACTION	99.2214532872
5.0886-5.8248	0.270337678412	142	0.262470704742	14846	X-RAY DIFFRACTION	99.1991528228
5.8248-7.3385	0.258102129725	138	0.270834333665	14919	X-RAY DIFFRACTION	99.3140294176
7.3385-111.4824	0.229864698053	142	0.222538924712	15025	X-RAY DIFFRACTION	97.8958239205