+
Open data
-
Basic information
Entry | Database: PDB / ID: 6in7 | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of AlgU in complex with MucA(cyto) | ||||||
![]() |
| ||||||
![]() | TRANSCRIPTION / Sigma factor | ||||||
Function / homology | ![]() positive regulation of cellular response to heat / regulation of polysaccharide biosynthetic process / positive regulation of response to oxidative stress / positive regulation of cell adhesion involved in single-species biofilm formation / positive regulation of single-species biofilm formation on inanimate substrate / negative regulation of bacterial-type flagellum-dependent cell motility / alginic acid biosynthetic process / sigma factor antagonist activity / cellular response to antibiotic / DNA-binding transcription activator activity ...positive regulation of cellular response to heat / regulation of polysaccharide biosynthetic process / positive regulation of response to oxidative stress / positive regulation of cell adhesion involved in single-species biofilm formation / positive regulation of single-species biofilm formation on inanimate substrate / negative regulation of bacterial-type flagellum-dependent cell motility / alginic acid biosynthetic process / sigma factor antagonist activity / cellular response to antibiotic / DNA-binding transcription activator activity / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / sigma factor activity / DNA-templated transcription initiation / protein-DNA complex / transcription cis-regulatory region binding / positive regulation of DNA-templated transcription / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Li, S. / Zhang, Q. / Bartlam, M. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Structural basis for the recognition of MucA by MucB and AlgU in Pseudomonas aeruginosa. Authors: Li, S. / Lou, X. / Xu, Y. / Teng, X. / Liu, R. / Zhang, Q. / Wu, W. / Wang, Y. / Bartlam, M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 72.4 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 52.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 450.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 452 KB | Display | |
Data in XML | ![]() | 15.2 KB | Display | |
Data in CIF | ![]() | 22.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6in8C ![]() 6in9C ![]() 1or7S S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 8908.094 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: mucA, PA0763 / Production host: ![]() ![]() |
---|---|
#2: Protein | Mass: 22227.156 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: algU, algT, PA0762 / Production host: ![]() ![]() |
#3: Chemical | ChemComp-NCA / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.65 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 2.0 M ammonium sulfate,0.1 M cacodylate pH 6.5, 0.2M sodium chloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 4, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.977853 Å / Relative weight: 1 |
Reflection | Resolution: 1.96→50 Å / Num. obs: 23934 / % possible obs: 99.5 % / Redundancy: 6.2 % / Rpim(I) all: 0.09 / Net I/σ(I): 27.9 |
Reflection shell | Resolution: 1.96→1.99 Å / Redundancy: 5.6 % / Mean I/σ(I) obs: 9.2 / Num. unique obs: 1053 / Rpim(I) all: 0.276 / % possible all: 90.6 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 1OR7 Resolution: 1.96→45.505 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 19.04
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.96→45.505 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|