+Open data
-Basic information
Entry | Database: PDB / ID: 6in7 | ||||||
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Title | Crystal structure of AlgU in complex with MucA(cyto) | ||||||
Components |
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Keywords | TRANSCRIPTION / Sigma factor | ||||||
Function / homology | Function and homology information positive regulation of cellular response to heat / regulation of polysaccharide biosynthetic process / positive regulation of response to oxidative stress / negative regulation of bacterial-type flagellum-dependent cell motility / positive regulation of cell adhesion involved in single-species biofilm formation / positive regulation of single-species biofilm formation on inanimate substrate / alginic acid biosynthetic process / sigma factor antagonist activity / cellular response to antibiotic / DNA-binding transcription activator activity ...positive regulation of cellular response to heat / regulation of polysaccharide biosynthetic process / positive regulation of response to oxidative stress / negative regulation of bacterial-type flagellum-dependent cell motility / positive regulation of cell adhesion involved in single-species biofilm formation / positive regulation of single-species biofilm formation on inanimate substrate / alginic acid biosynthetic process / sigma factor antagonist activity / cellular response to antibiotic / DNA-binding transcription activator activity / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / sigma factor activity / protein-DNA complex / DNA-templated transcription initiation / transcription cis-regulatory region binding / positive regulation of DNA-templated transcription / plasma membrane Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.96 Å | ||||||
Authors | Li, S. / Zhang, Q. / Bartlam, M. | ||||||
Funding support | China, 1items
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Citation | Journal: Febs J. / Year: 2019 Title: Structural basis for the recognition of MucA by MucB and AlgU in Pseudomonas aeruginosa. Authors: Li, S. / Lou, X. / Xu, Y. / Teng, X. / Liu, R. / Zhang, Q. / Wu, W. / Wang, Y. / Bartlam, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6in7.cif.gz | 72.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6in7.ent.gz | 52.5 KB | Display | PDB format |
PDBx/mmJSON format | 6in7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/in/6in7 ftp://data.pdbj.org/pub/pdb/validation_reports/in/6in7 | HTTPS FTP |
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-Related structure data
Related structure data | 6in8C 6in9C 1or7S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 8908.094 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria) Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: mucA, PA0763 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P38107 |
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#2: Protein | Mass: 22227.156 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (bacteria) Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: algU, algT, PA0762 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q06198 |
#3: Chemical | ChemComp-NCA / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.65 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 2.0 M ammonium sulfate,0.1 M cacodylate pH 6.5, 0.2M sodium chloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.977853 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 4, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.977853 Å / Relative weight: 1 |
Reflection | Resolution: 1.96→50 Å / Num. obs: 23934 / % possible obs: 99.5 % / Redundancy: 6.2 % / Rpim(I) all: 0.09 / Net I/σ(I): 27.9 |
Reflection shell | Resolution: 1.96→1.99 Å / Redundancy: 5.6 % / Mean I/σ(I) obs: 9.2 / Num. unique obs: 1053 / Rpim(I) all: 0.276 / % possible all: 90.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1OR7 Resolution: 1.96→45.505 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 19.04
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.96→45.505 Å
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Refine LS restraints |
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LS refinement shell |
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