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Open data
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Basic information
Entry | Database: PDB / ID: 6in9 | ||||||
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Title | Crystal structure of MucB in complex with MucA(peri) | ||||||
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![]() | MEMBRANE PROTEIN / Outer membrane protein | ||||||
Function / homology | ![]() regulation of polysaccharide biosynthetic process / antisigma factor binding / alginic acid biosynthetic process / sigma factor antagonist activity / outer membrane-bounded periplasmic space / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Li, S. / Zhang, Q. / Bartlam, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for the recognition of MucA by MucB and AlgU in Pseudomonas aeruginosa. Authors: Li, S. / Lou, X. / Xu, Y. / Teng, X. / Liu, R. / Zhang, Q. / Wu, W. / Wang, Y. / Bartlam, M. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 146.7 KB | Display | ![]() |
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PDB format | ![]() | 112.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 457 KB | Display | ![]() |
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Full document | ![]() | 464.6 KB | Display | |
Data in XML | ![]() | 28.7 KB | Display | |
Data in CIF | ![]() | 41.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6in7C ![]() 6in8C ![]() 2p4bS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32440.434 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: mucB, algN, PA0764 / Production host: ![]() ![]() #2: Protein | Mass: 9712.626 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: mucA, PA0763 / Production host: ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 37.86 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2 M Potassium citrate tribasic monohydrate, 20% w/v polyethylene glycol 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 30, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97853 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. obs: 61207 / % possible obs: 99.8 % / Redundancy: 3.3 % / Rpim(I) all: 0.044 / Net I/σ(I): 17.8 |
Reflection shell | Resolution: 1.8→1.83 Å / Redundancy: 3 % / Mean I/σ(I) obs: 2.1 / Num. unique obs: 3024 / Rpim(I) all: 0.262 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2P4B Resolution: 1.801→44.396 Å / SU ML: 0.2 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 22.22
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.801→44.396 Å
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Refine LS restraints |
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LS refinement shell |
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