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- PDB-6hoh: Structure of VPS34 LIR motif (S249E) bound to GABARAP -

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Basic information

Entry
Database: PDB / ID: 6hoh
TitleStructure of VPS34 LIR motif (S249E) bound to GABARAP
ComponentsPhosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein
KeywordsSIGNALING PROTEIN / Autophagy / ATG8 / LIR
Function / homology
Function and homology information


Toll Like Receptor 9 (TLR9) Cascade / protein lipidation / Synthesis of PIPs at the late endosome membrane / Synthesis of PIPs at the early endosome membrane / phosphatidylinositol 3-kinase complex, class III / phosphatidylinositol 3-kinase complex, class III, type II / phosphatidylinositol 3-kinase complex, class III, type I / positive regulation of protein K48-linked ubiquitination / positive regulation by host of viral genome replication / Synthesis of PIPs at the Golgi membrane ...Toll Like Receptor 9 (TLR9) Cascade / protein lipidation / Synthesis of PIPs at the late endosome membrane / Synthesis of PIPs at the early endosome membrane / phosphatidylinositol 3-kinase complex, class III / phosphatidylinositol 3-kinase complex, class III, type II / phosphatidylinositol 3-kinase complex, class III, type I / positive regulation of protein K48-linked ubiquitination / positive regulation by host of viral genome replication / Synthesis of PIPs at the Golgi membrane / regulation of Rac protein signal transduction / phosphatidylinositol kinase activity / protein localization to phagophore assembly site / GABA receptor binding / protein targeting to lysosome / early endosome to late endosome transport / cellular response to nitrogen starvation / phosphatidylethanolamine binding / phagophore assembly site / phosphatidylinositol-mediated signaling / Translation of Replicase and Assembly of the Replication Transcription Complex / TBC/RABGAPs / microtubule associated complex / autolysosome / phosphatidylinositol 3-kinase / phosphatidylinositol-3-phosphate biosynthetic process / 1-phosphatidylinositol-3-kinase activity / Macroautophagy / beta-tubulin binding / smooth endoplasmic reticulum / axoneme / autophagosome maturation / autophagosome membrane / phosphatidylinositol phosphate biosynthetic process / autophagosome assembly / extrinsic apoptotic signaling pathway via death domain receptors / RHO GTPases Activate NADPH Oxidases / PI3K Cascade / autophagosome / protein targeting / regulation of macroautophagy / cellular response to glucose starvation / sperm midpiece / regulation of cytokinesis / regulation of autophagy / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / macroautophagy / microtubule cytoskeleton organization / autophagy / peroxisome / endocytosis / phagocytic vesicle membrane / actin cytoskeleton / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / protein transport / late endosome / Translation of Replicase and Assembly of the Replication Transcription Complex / kinase activity / midbody / chemical synaptic transmission / cytoplasmic vesicle / microtubule binding / microtubule / lysosome / endosome / protein kinase activity / cell cycle / cell division / phosphorylation / Golgi membrane / synapse / ubiquitin protein ligase binding / SARS-CoV-2 activates/modulates innate and adaptive immune responses / ATP binding / membrane / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Phosphatidylinositol 3-kinase, Vps34 type / Autophagy protein Atg8 ubiquitin-like / Autophagy protein Atg8 ubiquitin like / Phosphoinositide 3-kinase C2 / Phosphoinositide 3-kinase, region postulated to contain C2 domain / C2 phosphatidylinositol 3-kinase-type domain / C2 phosphatidylinositol 3-kinase (PI3K)-type domain profile. / Phosphoinositide 3-kinase, accessory (PIK) domain superfamily / Phosphoinositide 3-kinase family, accessory domain (PIK domain) / Phosphoinositide 3-kinase family, accessory domain (PIK domain) ...Phosphatidylinositol 3-kinase, Vps34 type / Autophagy protein Atg8 ubiquitin-like / Autophagy protein Atg8 ubiquitin like / Phosphoinositide 3-kinase C2 / Phosphoinositide 3-kinase, region postulated to contain C2 domain / C2 phosphatidylinositol 3-kinase-type domain / C2 phosphatidylinositol 3-kinase (PI3K)-type domain profile. / Phosphoinositide 3-kinase, accessory (PIK) domain superfamily / Phosphoinositide 3-kinase family, accessory domain (PIK domain) / Phosphoinositide 3-kinase family, accessory domain (PIK domain) / Phosphoinositide 3-kinase, accessory (PIK) domain / Phosphatidylinositol kinase / PIK helical domain profile. / Phosphatidylinositol 3- and 4-kinases signature 1. / Phosphatidylinositol 3/4-kinase, conserved site / Phosphatidylinositol 3- and 4-kinases signature 2. / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / C2 domain superfamily / Ubiquitin-like (UB roll) / Armadillo-type fold / Ubiquitin-like domain superfamily / Roll / Protein kinase-like domain superfamily / Alpha Beta
Similarity search - Domain/homology
TRIETHYLENE GLYCOL / Gamma-aminobutyric acid receptor-associated protein / Phosphatidylinositol 3-kinase catalytic subunit type 3
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.25 Å
AuthorsMouilleron, S. / Birgisdottir, A.B. / Bhujbal, Z. / Wirth, M. / Sjottem, E. / Evjen, G. / Zhang, W. / Lee, R. / O'Reilly, N. / Tooze, S. ...Mouilleron, S. / Birgisdottir, A.B. / Bhujbal, Z. / Wirth, M. / Sjottem, E. / Evjen, G. / Zhang, W. / Lee, R. / O'Reilly, N. / Tooze, S. / Lamark, T. / Johansen, T.
Funding support Norway, 2items
OrganizationGrant numberCountry
Research Council of Norway214448 Norway
Research Council of Norway249884 Norway
CitationJournal: Autophagy / Year: 2019
Title: Members of the autophagy class III phosphatidylinositol 3-kinase complex I interact with GABARAP and GABARAPL1 via LIR motifs.
Authors: Birgisdottir, A.B. / Mouilleron, S. / Bhujabal, Z. / Wirth, M. / Sjottem, E. / Evjen, G. / Zhang, W. / Lee, R. / O'Reilly, N. / Tooze, S.A. / Lamark, T. / Johansen, T.
History
DepositionSep 17, 2018Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 27, 2019Provider: repository / Type: Initial release
Revision 1.1Mar 13, 2019Group: Data collection / Database references / Category: citation / citation_author / pdbx_database_proc
Item: _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.2Jul 10, 2019Group: Data collection / Database references / Category: citation / pdbx_database_proc
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Jan 24, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein
B: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein
C: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein
D: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)61,0845
Polymers60,9344
Non-polymers1501
Water2,882160
1
A: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein
hetero molecules

A: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein
hetero molecules

A: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)46,1516
Polymers45,7013
Non-polymers4513
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation5_555z,x,y1
crystal symmetry operation9_555y,z,x1
Buried area6650 Å2
ΔGint-26 kcal/mol
Surface area19790 Å2
MethodPISA
2
B: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein
C: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein

D: Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein


Theoretical massNumber of molelcules
Total (without water)45,7013
Polymers45,7013
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation11_556y+1/2,-z+1/2,-x+11
Buried area5870 Å2
ΔGint-43 kcal/mol
Surface area19930 Å2
MethodPISA
Unit cell
Length a, b, c (Å)119.502, 119.502, 119.502
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number198
Space group name H-MP213

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Components

#1: Protein
Phosphatidylinositol 3-kinase catalytic subunit type 3,Gamma-aminobutyric acid receptor-associated protein / PtdIns-3-kinase type 3 / Phosphatidylinositol 3-kinase p100 subunit / Phosphoinositide-3-kinase ...PtdIns-3-kinase type 3 / Phosphatidylinositol 3-kinase p100 subunit / Phosphoinositide-3-kinase class 3 / hVps34 / GABA(A) receptor-associated protein / MM46


Mass: 15233.520 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PIK3C3, VPS34, GABARAP, FLC3B, HT004 / Production host: Escherichia coli (E. coli)
References: UniProt: Q8NEB9, UniProt: O95166, phosphatidylinositol 3-kinase
#2: Chemical ChemComp-PGE / TRIETHYLENE GLYCOL / Polyethylene glycol


Mass: 150.173 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H14O4
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 160 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.4 Å3/Da / Density % sol: 48.77 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 20% PEG 3350, 200 mM magnesium formate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.97 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Mar 12, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97 Å / Relative weight: 1
ReflectionResolution: 2.25→53.443 Å / Num. obs: 27219 / % possible obs: 99.49 % / Redundancy: 5.8 % / Biso Wilson estimate: 39 Å2 / CC1/2: 0.99 / Rmerge(I) obs: 0.08 / Rpim(I) all: 0.03 / Rrim(I) all: 0.09 / Net I/σ(I): 10.4
Reflection shellResolution: 2.25→2.33 Å / Redundancy: 5.9 % / Rmerge(I) obs: 0.98 / Num. unique obs: 2654 / CC1/2: 0.65 / Rpim(I) all: 0.44 / Rrim(I) all: 1.07 / Χ2: 39 / % possible all: 99.21

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Processing

Software
NameVersionClassification
PHENIX(1.12_2829: ???)refinement
xia2data reduction
DIALSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1GNU

1gnu


Resolution: 2.25→53.44 Å / SU ML: 0.37 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 30.25
RfactorNum. reflection% reflection
Rfree0.2496 1272 4.7 %
Rwork0.2064 --
obs0.2084 27048 99.31 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.25→53.44 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4153 0 10 160 4323
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0024320
X-RAY DIFFRACTIONf_angle_d0.4225843
X-RAY DIFFRACTIONf_dihedral_angle_d16.4792636
X-RAY DIFFRACTIONf_chiral_restr0.044616
X-RAY DIFFRACTIONf_plane_restr0.003761
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.2504-2.34050.37711310.34392813X-RAY DIFFRACTION99
2.3405-2.4470.42951610.32822774X-RAY DIFFRACTION99
2.447-2.5760.35781500.30942812X-RAY DIFFRACTION99
2.576-2.73740.31871340.28712870X-RAY DIFFRACTION100
2.7374-2.94870.29761350.25762843X-RAY DIFFRACTION100
2.9487-3.24540.32021460.21742881X-RAY DIFFRACTION100
3.2454-3.71490.22111580.192855X-RAY DIFFRACTION100
3.7149-4.680.16881070.14372943X-RAY DIFFRACTION100
4.68-53.4580.1941500.17112985X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.4050.02170.8679-0.1138-0.02791.2621-1.08780.65570.02670.91210.61710.32540.97680.11590.00430.66480.00130.0380.37850.03730.62597.41422.672333.9808
20.12440.09940.1208-0.00190.12830.1905-0.5152-1.09120.16890.23550.58020.1467-0.11670.160300.41420.0280.00070.59-0.03460.456224.8456-4.694422.5499
30.1355-0.24080.18190.321-0.04970.12310.9355-0.2555-1.3505-0.1866-0.2422-0.05830.21450.03490.00040.4934-0.1048-0.11480.54310.08250.556434.464-8.289118.3085
40.3959-0.3565-0.22210.57180.15390.00610.0952-0.0429-0.0346-0.211-0.06890.0340.19750.5269-00.35570.0325-0.06290.52080.00870.316243.62491.373516.6996
5-0.01050.17090.2632-0.16110.30551.27850.1977-0.11130.19350.07-0.01480.3674-0.04970.0564-0.00010.29270.0052-0.0010.3718-0.02410.359734.48925.17211.2996
60.3748-0.85210.46620.6204-0.81320.2743-0.1502-0.2035-1.02070.30920.0073-0.0550.2758-0.1364-0.00010.4424-0.02420.0180.4574-0.06450.553420.8462-4.906510.3895
70.17160.0927-0.12710.02070.05780.07580.10660.52780.2134-0.08780.01490.4853-0.02970.560400.36250.0433-0.00260.4619-0.04170.441432.71019.30498.6733
80.0972-0.04470.2290.3243-0.05210.1940.04610.53670.42910.0663-0.2245-0.01420.1403-0.1976-00.3777-0.0004-0.03120.403-0.00220.40822.70637.92895.7285
9-0.0489-0.00760.2931-0.02310.133-0.2077-0.1953-0.28570.0175-0.15950.00630.1096-0.11530.0373-00.3496-0.0192-0.01440.4653-0.04180.420515.01962.74938.1452
100.1077-0.3770.19850.74880.43870.6164-0.2713-0.85430.34940.78680.08410.248-0.47780.2028-0.00010.42120.0897-0.02230.5012-0.06020.51623.238211.072618.9441
110.0567-0.174-0.04060.2954-0.04910.10770.2771-0.0220.74780.1722-0.49330.0219-0.1402-0.3791-0.00020.3484-0.08970.02720.4373-0.03710.422732.113412.312519.0682
120.4013-0.1728-0.26910.4358-1.58461.0711-0.05870.12780.14270.0048-0.01420.2137-0.20690.1039-0.00010.3189-0.0022-0.02660.3591-0.05060.340829.0572.269418.2668
130.07920.09150.04270.04380.0468-0.02940.5059-1.11761.43670.25110.3313-2.2177-1.18540.19110.00070.946-0.0828-0.09550.7131-0.01440.706454.572840.184363.0743
141.9121-0.3826-0.80540.1871-0.12990.55450.5076-0.5794-0.3435-0.57250.3168-1.19550.45090.8545-0.02880.53910.00430.1230.28280.02020.560457.520631.901151.003
150.01840.04370.04640.0562-0.07210.05871.20970.9462-0.00050.16480.04580.9348-0.8497-0.34930.00030.6967-0.0641-0.040.52730.0260.670864.73220.890737.2596
160.2455-0.2113-0.8122.5311.70713.7921-0.63512.21480.20070.3833-0.832-0.37851.5171.5196-0.10190.3547-0.1870.06540.95850.27960.633469.960516.42427.837
171.0057-0.6250.44070.41230.62850.595-0.0637-0.06780.0431-0.4405-0.4415-0.5261-0.33110.6252-1.01720.515-0.1308-0.04190.80470.19930.452161.289515.807918.0709
180.1056-0.1012-0.38471.0686-0.70950.576-0.14760.2682-0.03350.1626-0.04070.1865-0.53010.3707-00.2957-0.0276-0.02620.43640.02010.33356.274510.441626.4347
191.32941.6182-1.31890.2155-0.32051.08140.3606-0.0746-0.0741-0.1484-0.11070.038-0.07740.20380.00090.2909-0.0343-0.01870.3713-0.010.273159.2218.284434.9444
200.2592-0.42710.07350.1960.35470.5999-0.11360.09920.1468-0.4901-0.03150.07790.3869-0.03090.00010.2814-0.01750.01310.37990.02290.319852.31124.472838.1457
210.23640.3090.31210.1274-0.28430.27790.1835-0.12460.04810.1414-0.15140.0599-0.25950.2367-00.4172-0.00090.0260.3933-0.02450.35858.2796.885846.5229
22-0.02230.13230.11530.2654-0.5410.25070.3521-0.3650.7204-0.3337-0.1361-0.2496-0.5509-0.91330.00020.43120.13880.02360.50180.04260.520149.786917.966737.6124
230.1367-0.04380.14730.1890.0244-0.0341-0.18070.28670.50250.2137-0.16840.1904-0.226-0.30070.00020.48570.0217-0.09030.46840.05410.429849.472118.40228.4255
240.38780.0119-0.33020.0231-0.12150.0967-0.92031.19190.96140.77410.0758-0.5639-1.25740.5684-0.17650.7452-0.0895-0.07340.59990.29960.567358.738420.450724.0064
250.483-0.26630.50770.57370.34720.28240.19040.01950.21790.36770.1058-0.066-0.7360.8943-0.00020.4126-0.0673-0.08080.38170.03260.445459.300214.669239.8672
262.256-0.68870.63932.7834-1.1981.8508-0.4150.6090.3325-0.47060.5032-0.79210.7287-0.31940.19980.3216-0.03140.02080.5303-0.02930.135454.82592.035725.6825
270.07540.02820.06070.424-0.08260.28970.97911.3824-0.06170.39970.00940.208-0.99260.56620.00550.33160.0783-0.00290.57370.1010.506635.7802-5.863436.6418
280.1777-0.1163-0.08080.28970.21580.07060.29150.1569-0.14220.4352-0.4282-0.14360.5566-0.8321-0.00010.4982-0.0067-0.04760.5735-0.02570.66125.7298-9.003540.1774
290.655-0.0645-0.1818-0.15530.25160.36590.188-0.2281-0.0184-0.03860.12280.09590.2337-0.27380.00010.47790.0148-0.06510.61280.06240.491917.4040.874143.0649
300.7025-0.14540.678-0.13360.39190.568-0.1972-0.0761-0.0024-0.15860.2211-0.1552-0.1949-0.0267-0.00010.35480.0149-0.02310.41020.05250.340326.96423.607848.5095
311.04460.35841.31361.153-0.78673.8381-0.2311-0.10060.04290.35160.07280.41490.4179-0.1477-0.00010.2478-0.01270.01090.3673-0.04810.386634.4239-0.272749.8678
320.97880.27490.50610.6076-0.44750.289-0.1948-0.36590.54580.02350.2181-0.1169-0.0967-0.379100.41650.0013-0.02070.38530.01850.436239.04834.603253.8781
330.1811-0.24420.0261-0.0819-0.00630.5933-0.05230.19650.21960.2242-0.5112-0.16830.00230.466-0.00020.356-0.01330.03260.46910.06670.41746.4116-0.759251.2158
340.0809-0.26650.18250.6136-0.47810.3407-0.3060.26760.3022-0.95840.19740.1199-1.3120.7220.00010.4246-0.0493-0.0210.51880.12560.51538.71719.059740.9939
350.52240.44390.05050.21060.2210.59210.08220.3140.3522-0.4908-0.0774-0.0778-0.107-0.180300.38470.0821-0.040.50830.06930.419627.21887.682940.0303
360.1281-0.2144-0.0287-0.12070.3506-0.25360.0418-0.0494-0.1991-0.0802-0.3170.25690.30630.0246-0.00020.36360.0621-0.00260.41370.0540.338238.3997-0.989843.5645
370.62270.1465-0.3412-0.46-0.75680.1279-0.15760.1008-0.48830.00380.3116-0.066-0.4418-0.0836-0.00030.5227-0.08410.05210.4852-0.02120.707960.9829-30.789750.2174
380.0264-0.33830.17481.2337-0.29221.5697-0.10660.1521-0.4243-0.3395-0.0518-0.2767-0.0081-0.452900.5129-0.04350.08940.4462-0.04770.391259.8011-16.067921.4034
39-0.41631.11850.14213.3257-0.42243.2776-0.0832-0.0143-0.07880.17320.0556-0.0527-0.0768-0.1064-00.30880.0420.02650.454-0.02740.30461.2224-9.19938.5309
401.58980.8988-0.16112.39912.32612.7817-0.4689-0.1083-0.8315-0.19460.0714-0.30580.44950.4324-0.34370.39480.08060.17450.39630.09860.46566.2806-19.387832.9673
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid -13 through -2 )
2X-RAY DIFFRACTION2chain 'A' and (resid -1 through 3 )
3X-RAY DIFFRACTION3chain 'A' and (resid 4 through 10 )
4X-RAY DIFFRACTION4chain 'A' and (resid 11 through 24 )
5X-RAY DIFFRACTION5chain 'A' and (resid 25 through 35 )
6X-RAY DIFFRACTION6chain 'A' and (resid 36 through 47 )
7X-RAY DIFFRACTION7chain 'A' and (resid 48 through 56 )
8X-RAY DIFFRACTION8chain 'A' and (resid 57 through 67 )
9X-RAY DIFFRACTION9chain 'A' and (resid 68 through 79 )
10X-RAY DIFFRACTION10chain 'A' and (resid 80 through 90 )
11X-RAY DIFFRACTION11chain 'A' and (resid 91 through 98 )
12X-RAY DIFFRACTION12chain 'A' and (resid 99 through 112 )
13X-RAY DIFFRACTION13chain 'B' and (resid -16 through -11 )
14X-RAY DIFFRACTION14chain 'B' and (resid -10 through -2 )
15X-RAY DIFFRACTION15chain 'B' and (resid -1 through 3 )
16X-RAY DIFFRACTION16chain 'B' and (resid 4 through 10 )
17X-RAY DIFFRACTION17chain 'B' and (resid 11 through 24 )
18X-RAY DIFFRACTION18chain 'B' and (resid 25 through 35 )
19X-RAY DIFFRACTION19chain 'B' and (resid 36 through 56 )
20X-RAY DIFFRACTION20chain 'B' and (resid 57 through 67 )
21X-RAY DIFFRACTION21chain 'B' and (resid 68 through 79 )
22X-RAY DIFFRACTION22chain 'B' and (resid 80 through 90 )
23X-RAY DIFFRACTION23chain 'B' and (resid 91 through 98 )
24X-RAY DIFFRACTION24chain 'B' and (resid 99 through 104 )
25X-RAY DIFFRACTION25chain 'B' and (resid 105 through 112 )
26X-RAY DIFFRACTION26chain 'C' and (resid -14 through -2 )
27X-RAY DIFFRACTION27chain 'C' and (resid -1 through 3 )
28X-RAY DIFFRACTION28chain 'C' and (resid 4 through 10 )
29X-RAY DIFFRACTION29chain 'C' and (resid 11 through 24 )
30X-RAY DIFFRACTION30chain 'C' and (resid 25 through 35 )
31X-RAY DIFFRACTION31chain 'C' and (resid 36 through 56 )
32X-RAY DIFFRACTION32chain 'C' and (resid 57 through 67 )
33X-RAY DIFFRACTION33chain 'C' and (resid 68 through 79 )
34X-RAY DIFFRACTION34chain 'C' and (resid 80 through 90 )
35X-RAY DIFFRACTION35chain 'C' and (resid 91 through 104 )
36X-RAY DIFFRACTION36chain 'C' and (resid 105 through 112 )
37X-RAY DIFFRACTION37chain 'D' and (resid -13 through 3 )
38X-RAY DIFFRACTION38chain 'D' and (resid 4 through 35 )
39X-RAY DIFFRACTION39chain 'D' and (resid 36 through 76 )
40X-RAY DIFFRACTION40chain 'D' and (resid 77 through 112 )

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