[English] 日本語

- PDB-6h19: Crystal structure of ethyl-paraoxon inhibited recombinant human b... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 6h19 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Crystal structure of ethyl-paraoxon inhibited recombinant human bile salt activated lipase (aged form) | |||||||||
![]() | Bile salt-activated lipase | |||||||||
![]() | ![]() ![]() | |||||||||
Function / homology | ![]() protein esterification / intestinal lipid catabolic process / ceramide catabolic process / Digestion of dietary lipid / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Touvrey, C. / Brazzolotto, X. / Nachon, F. | |||||||||
Funding support | ![]()
| |||||||||
![]() | ![]() Title: X-ray structures of human bile-salt activated lipase conjugated to nerve agents surrogates. Authors: Touvrey, C. / Courageux, C. / Guillon, V. / Terreux, R. / Nachon, F. / Brazzolotto, X. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 121.5 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 94.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 304.9 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 308.8 KB | Display | |
Data in XML | ![]() | 22.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 60878.801 Da / Num. of mol.: 1 / Mutation: N186D, A298D, 534STOP Source method: isolated from a genetically manipulated source Details: SPT = serine residue inhibited by ethylparaoxon in its aged form Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: P19835, ![]() ![]() | ||||||
---|---|---|---|---|---|---|---|
#2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-ACT / ![]() #4: Chemical | ChemComp-NA / | #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.46 % |
---|---|
Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: PEG 8000, cacodylate, zinc acetate |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 X 2M / Detector: PIXEL / Date: Mar 14, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.89→44.7 Å / Num. obs: 49065 / % possible obs: 99.35 % / Redundancy: 3.8 % / CC1/2: 0.995 / Rmerge(I) obs: 0.1196 / Rrim(I) all: 0.1387 / Net I/σ(I): 8.21 |
Reflection shell | Resolution: 1.89→1.958 Å / Redundancy: 3.8 % / Mean I/σ(I) obs: 1.37 / Num. unique obs: 4813 / CC1/2: 0.639 / % possible all: 98.75 |
-
Processing
Software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.89→44.7 Å / Cross valid method: FREE R-VALUE
| ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.89→44.7 Å
|