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- PDB-1aql: CRYSTAL STRUCTURE OF BOVINE BILE-SALT ACTIVATED LIPASE COMPLEXED ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1aql | ||||||
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Title | CRYSTAL STRUCTURE OF BOVINE BILE-SALT ACTIVATED LIPASE COMPLEXED WITH TAUROCHOLATE | ||||||
![]() | BILE-SALT ACTIVATED LIPASE | ||||||
![]() | HYDROLASE / SERINE ESTERASE / LIPID DEGRADATION / GLYCOPROTEIN | ||||||
Function / homology | ![]() retinyl-palmitate esterase activity / acetylesterase / ceramide catabolic process / sterol esterase / sterol ester esterase activity / pancreatic juice secretion / postsynaptic membrane assembly / presynaptic membrane assembly / acetylesterase activity / neuron cell-cell adhesion ...retinyl-palmitate esterase activity / acetylesterase / ceramide catabolic process / sterol esterase / sterol ester esterase activity / pancreatic juice secretion / postsynaptic membrane assembly / presynaptic membrane assembly / acetylesterase activity / neuron cell-cell adhesion / neurexin family protein binding / triacylglycerol lipase / triacylglycerol lipase activity / synaptic vesicle endocytosis / modulation of chemical synaptic transmission / signaling receptor activity / chemical synaptic transmission / postsynaptic membrane / cell surface / extracellular region / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Wang, X. / Zhang, X. | ||||||
![]() | ![]() Title: The crystal structure of bovine bile salt activated lipase: insights into the bile salt activation mechanism. Authors: Wang, X. / Wang, C.S. / Tang, J. / Dyda, F. / Zhang, X.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 209.6 KB | Display | ![]() |
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PDB format | ![]() | 174.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 603.2 KB | Display | ![]() |
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Full document | ![]() | 661.9 KB | Display | |
Data in XML | ![]() | 30.9 KB | Display | |
Data in CIF | ![]() | 43.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1aknSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.352585, 0.46694, 0.810957), Vector: |
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Components
#1: Protein | Mass: 59065.461 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Sugar | #3: Chemical | ChemComp-TCH / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 61.5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: Mar 1, 1997 |
Radiation | Monochromator: CRYSTAL / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→17 Å / Num. obs: 33877 / % possible obs: 82.7 % / Observed criterion σ(I): 0.5 / Redundancy: 1.8 % / Biso Wilson estimate: 43.5 Å2 / Rsym value: 0.117 / Net I/σ(I): 9.1 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 1.3 % / Mean I/σ(I) obs: 2.4 / Rsym value: 0.206 / % possible all: 48.3 |
Reflection | *PLUS Rmerge(I) obs: 0.117 |
Reflection shell | *PLUS % possible obs: 48.3 % / Rmerge(I) obs: 0.206 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1AKN Resolution: 2.8→8 Å / σ(F): 1.5 Details: AT FINAL STEP, A RESTRAINED TEMPERATURE FACTOR REFINEMENT WAS CARRIED OUT BY TNT.
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Displacement parameters | Biso mean: 43.7 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→8 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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