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- PDB-6fg1: CRYSTAL STRUCTURE OF FAB OF NATALIZUMAB IN COMPLEX WITH FAB OF NAA32. -
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Open data
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Basic information
Entry | Database: PDB / ID: 6fg1 | ||||||
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Title | CRYSTAL STRUCTURE OF FAB OF NATALIZUMAB IN COMPLEX WITH FAB OF NAA32. | ||||||
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![]() | IMMUNE SYSTEM / ANTIBODY | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bertrand, T. / Pouzieux, S. | ||||||
![]() | ![]() Title: A single T cell epitope drives the neutralizing anti-drug antibody response to natalizumab in multiple sclerosis patients. Authors: Cassotta, A. / Mikol, V. / Bertrand, T. / Pouzieux, S. / Le Parc, J. / Ferrari, P. / Dumas, J. / Auer, M. / Deisenhammer, F. / Gastaldi, M. / Franciotta, D. / Silacci-Fregni, C. / Fernandez ...Authors: Cassotta, A. / Mikol, V. / Bertrand, T. / Pouzieux, S. / Le Parc, J. / Ferrari, P. / Dumas, J. / Auer, M. / Deisenhammer, F. / Gastaldi, M. / Franciotta, D. / Silacci-Fregni, C. / Fernandez Rodriguez, B. / Giacchetto-Sasselli, I. / Foglierini, M. / Jarrossay, D. / Geiger, R. / Sallusto, F. / Lanzavecchia, A. / Piccoli, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 185.7 KB | Display | ![]() |
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PDB format | ![]() | 145.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 464.2 KB | Display | ![]() |
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Full document | ![]() | 468.9 KB | Display | |
Data in XML | ![]() | 32.8 KB | Display | |
Data in CIF | ![]() | 46.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6fg2C ![]() 3x3fS ![]() 4irzS ![]() 4nm4S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Antibody , 4 types, 4 molecules ADHL
#1: Antibody | Mass: 26127.299 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Antibody | Mass: 23368.924 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Antibody | Mass: 25427.408 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#4: Antibody | Mass: 23579.217 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
-Non-polymers , 2 types, 256 molecules ![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.04 Å3/Da / Density % sol: 69.52 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / Details: Na2 Malonate 1.7M - pH6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Aug 25, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.966 Å / Relative weight: 1 |
Reflection | Resolution: 2.03→87.6 Å / Num. obs: 81847 / % possible obs: 99.8 % / Redundancy: 8 % / Biso Wilson estimate: 37.63 Å2 / Rmerge(I) obs: 0.132 / Net I/σ(I): 11.9 |
Reflection shell | Resolution: 2.03→2.07 Å |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: 4IRZ, 4NM4 and 3X3F Resolution: 2.03→87.26 Å / Cor.coef. Fo:Fc: 0.884 / Cor.coef. Fo:Fc free: 0.865 / SU R Cruickshank DPI: 0.28 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.279 / SU Rfree Blow DPI: 0.222 / SU Rfree Cruickshank DPI: 0.224
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Displacement parameters | Biso mean: 39.09 Å2
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Refine analyze | Luzzati coordinate error obs: 0.43 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.03→87.26 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.05 Å / Total num. of bins used: 20
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