根拠: mass spectrometry, Native Mass Spectrometry confirms the presence of the heterodimer in solution, gel filtration, Protein elutes as a monodisperse peak at the expected volume
構造決定の手法: 分子置換 開始モデル: Computational Design Model 解像度: 2.25→43.023 Å / SU ML: 0.31 / 交差検証法: FREE R-VALUE / σ(F): 0 / 位相誤差: 33.46 詳細: Iterative rounds of manual model building in Coot and refinement with Phenix Refine. Ultimately, resolution was trimmed to 2.25 from the high of the 1.83 diffraction data, as the high ...詳細: Iterative rounds of manual model building in Coot and refinement with Phenix Refine. Ultimately, resolution was trimmed to 2.25 from the high of the 1.83 diffraction data, as the high resolution data, as evidenced by the refinement R-factors, was deemed too weak to be useful.