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Open data
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Basic information
Entry | Database: PDB / ID: 6asd | ||||||
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Title | Zinc finger region of human TET1 in complex with CpG DNA | ||||||
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![]() | DNA BINDING PROTEIN/DNA / zinc finger / dna-binding / Structural Genomics / Structural Genomics Consortium / SGC / DNA BINDING PROTEIN-DNA complex | ||||||
Function / homology | ![]() : / : / 5-methylcytosine catabolic process / 5-methylcytosine dioxygenase activity / TET1,2,3 and TDG demethylate DNA / inner cell mass cell differentiation / : / negative regulation of DNA methylation-dependent heterochromatin formation / oxidative demethylation / protein O-linked glycosylation ...: / : / 5-methylcytosine catabolic process / 5-methylcytosine dioxygenase activity / TET1,2,3 and TDG demethylate DNA / inner cell mass cell differentiation / : / negative regulation of DNA methylation-dependent heterochromatin formation / oxidative demethylation / protein O-linked glycosylation / methyl-CpG binding / stem cell population maintenance / chromatin organization / iron ion binding / positive regulation of cell population proliferation / positive regulation of transcription by RNA polymerase II / DNA binding / zinc ion binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, K. / Xu, C. / Tempel, W. / Walker, J.R. / Arrowsmith, C.H. / Bountra, C. / Edwards, A.M. / Min, J. / Structural Genomics Consortium (SGC) | ||||||
![]() | ![]() Title: DNA Sequence Recognition of Human CXXC Domains and Their Structural Determinants. Authors: Xu, C. / Liu, K. / Lei, M. / Yang, A. / Li, Y. / Hughes, T.R. / Min, J. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 62 KB | Display | ![]() |
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PDB format | ![]() | 42.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 418.7 KB | Display | ![]() |
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Full document | ![]() | 418.7 KB | Display | |
Data in XML | ![]() | 5.8 KB | Display | |
Data in CIF | ![]() | 7.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4nw3C ![]() 4o64C ![]() 4pziC ![]() 4z3cC ![]() 5vc9C ![]() 5w9qC ![]() 5w9sC ![]() 6asbC ![]() 4hp3S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 3664.380 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: synthetic / Source: (synth.) synthetic construct (others) #2: Protein/peptide | | Mass: 5504.574 Da / Num. of mol.: 1 / Fragment: Zinc finger region (UNP residues 587-632) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q8NFU7, Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor #3: Chemical | ChemComp-UNX / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.2 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion / pH: 7.5 / Details: 25% PEG-3350, 0.2 M ammonium acetate, 0.1 M HEPES |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 20, 2013 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 1.85→36.72 Å / Num. obs: 11082 / % possible obs: 98.3 % / Redundancy: 3.6 % / CC1/2: 1 / Rmerge(I) obs: 0.038 / Rpim(I) all: 0.023 / Rrim(I) all: 0.044 / Net I/σ(I): 22.1 / Num. measured all: 40255 / Scaling rejects: 2 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 4hp3 Resolution: 1.85→36.72 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.95 / SU B: 5.58 / SU ML: 0.095 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.14 / ESU R Free: 0.131 Details: coot was used for interactive model building. Model geometry was assessed on the molprobity server.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 62.8 Å2 / Biso mean: 36.226 Å2 / Biso min: 22.32 Å2
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Refinement step | Cycle: final / Resolution: 1.85→36.72 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.85→1.898 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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