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Yorodumi- PDB-6a6h: Crystal Structure of Swine Major Histocompatibility Complex Class... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6a6h | ||||||
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Title | Crystal Structure of Swine Major Histocompatibility Complex Class I SLA-2*040202 For 2.3 Angstrom | ||||||
Components |
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Keywords | IMMUNE SYSTEM / MHC / Immunology / Immune system- transferase complex | ||||||
Function / homology | Function and homology information ER-Phagosome pathway / Endosomal/Vacuolar pathway / DAP12 interactions / DAP12 signaling / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / L-peptidase / IRES-dependent viral translational initiation / modulation by virus of host chromatin organization / Neutrophil degranulation ...ER-Phagosome pathway / Endosomal/Vacuolar pathway / DAP12 interactions / DAP12 signaling / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / L-peptidase / IRES-dependent viral translational initiation / modulation by virus of host chromatin organization / Neutrophil degranulation / antigen processing and presentation of peptide antigen via MHC class I / antigen processing and presentation / ribonucleoside triphosphate phosphatase activity / picornain 3C / T=pseudo3 icosahedral viral capsid / host cell cytoplasmic vesicle membrane / MHC class I protein complex / peptide antigen assembly with MHC class II protein complex / MHC class II protein complex / peptide antigen binding / : / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / positive regulation of T cell activation / nucleoside-triphosphate phosphatase / regulation of translation / protein complex oligomerization / MHC class II protein complex binding / late endosome membrane / monoatomic ion channel activity / clathrin-dependent endocytosis of virus by host cell / RNA helicase activity / membrane => GO:0016020 / viral protein processing / immune response / induction by virus of host autophagy / RNA-directed RNA polymerase / lysosomal membrane / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / DNA-templated transcription / virion attachment to host cell / structural molecule activity / proteolysis / RNA binding / extracellular region / ATP binding / membrane Similarity search - Function | ||||||
Biological species | Sus scrofa (pig) Foot-and-mouth disease virus - type A | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.31 Å | ||||||
Authors | Ning, S. / Wang, Z.B. | ||||||
Citation | Journal: Res. Vet. Sci. / Year: 2019 Title: Crystallization of SLA-2*04:02:02 complexed with a CTL epitope derived from FMDV. Authors: Ning, S. / Wang, Z.B. / Qi, P. / Xiao, J. / Wang, X.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6a6h.cif.gz | 172.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6a6h.ent.gz | 136.8 KB | Display | PDB format |
PDBx/mmJSON format | 6a6h.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a6/6a6h ftp://data.pdbj.org/pub/pdb/validation_reports/a6/6a6h | HTTPS FTP |
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-Related structure data
Related structure data | 5h94S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 31547.789 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sus scrofa (pig) / Gene: SLA-B, SLA-2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8MHU4 |
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#2: Protein | Mass: 11431.918 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sus scrofa (pig) / Gene: B2M / Production host: Escherichia coli (E. coli) / References: UniProt: Q07717 |
#3: Protein/peptide | Mass: 1031.247 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Foot-and-mouth disease virus - type A / References: UniProt: P49303*PLUS |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.73 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 0.1 M sodium acetate pH4.6 25% polyethylene glycol 4,000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1 Å |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Feb 15, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.31→88.16 Å / Num. obs: 19686 / % possible obs: 89.9 % / Redundancy: 11.1 % / Rmerge(I) obs: 0.01538 / Net I/σ(I): 22.96 |
Reflection shell | Resolution: 2.31→2.5 Å / Rmerge(I) obs: 0.099 / Num. unique obs: 19686 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5H94 Resolution: 2.31→88.16 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.879 / SU B: 17.316 / SU ML: 0.209 / Cross valid method: THROUGHOUT / ESU R: 0.456 / ESU R Free: 0.298 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 40.541 Å2
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Refinement step | Cycle: 1 / Resolution: 2.31→88.16 Å
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