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- PDB-5utz: Human IL-2/Fab complex -

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Open data


ID or keywords:

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Basic information

Entry
Database: PDB / ID: 5utz
TitleHuman IL-2/Fab complex
Components
  • Fab 5111 heavy chain
  • Fab 5111 light chain
  • Interleukin-2
KeywordsCYTOKINE/IMMUNE SYSTEM / cytokine / Fab / complex / CYTOKINE-IMMUNE SYSTEM complex
Function / homology
Function and homology information


kappa-type opioid receptor binding / response to tacrolimus / regulation of T cell homeostatic proliferation / regulation of CD4-positive, alpha-beta T cell proliferation / interleukin-2 receptor binding / glycosphingolipid binding / positive regulation of plasma cell differentiation / negative regulation of lymphocyte proliferation / positive regulation of tissue remodeling / negative regulation of T-helper 17 cell differentiation ...kappa-type opioid receptor binding / response to tacrolimus / regulation of T cell homeostatic proliferation / regulation of CD4-positive, alpha-beta T cell proliferation / interleukin-2 receptor binding / glycosphingolipid binding / positive regulation of plasma cell differentiation / negative regulation of lymphocyte proliferation / positive regulation of tissue remodeling / negative regulation of T-helper 17 cell differentiation / RUNX1 and FOXP3 control the development of regulatory T lymphocytes (Tregs) / leukocyte activation involved in immune response / positive regulation of isotype switching to IgG isotypes / interleukin-2-mediated signaling pathway / activated T cell proliferation / cell surface receptor signaling pathway via STAT / Interleukin-2 signaling / kinase activator activity / natural killer cell activation / positive regulation of regulatory T cell differentiation / negative regulation of B cell apoptotic process / positive regulation of immunoglobulin production / positive regulation of dendritic spine development / positive regulation of interleukin-17 production / positive regulation of activated T cell proliferation / T cell differentiation / Interleukin receptor SHC signaling / positive regulation of B cell proliferation / extrinsic apoptotic signaling pathway in absence of ligand / cytokine activity / growth factor activity / negative regulation of inflammatory response / positive regulation of type II interferon production / positive regulation of inflammatory response / cell-cell signaling / positive regulation of cytosolic calcium ion concentration / carbohydrate binding / RAF/MAP kinase cascade / positive regulation of cell growth / response to ethanol / phospholipase C-activating G protein-coupled receptor signaling pathway / adaptive immune response / transcription by RNA polymerase II / cell adhesion / immune response / positive regulation of cell population proliferation / negative regulation of apoptotic process / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region / plasma membrane
Similarity search - Function
Interleukin-2 / Interleukin-2, conserved site / Interleukin 2 / Interleukin-2 signature. / Interleukin-2 family / Growth Hormone; Chain: A; - #10 / Four-helical cytokine-like, core / Growth Hormone; Chain: A; / : / : ...Interleukin-2 / Interleukin-2, conserved site / Interleukin 2 / Interleukin-2 signature. / Interleukin-2 family / Growth Hormone; Chain: A; - #10 / Four-helical cytokine-like, core / Growth Hormone; Chain: A; / : / : / Immunoglobulin V-Type / Immunoglobulin V-set domain / Immunoglobulin V-set domain / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin subtype / Immunoglobulin / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold / Immunoglobulins / Up-down Bundle / Immunoglobulin-like / Sandwich / Mainly Beta / Mainly Alpha
Similarity search - Domain/homology
Interleukin-2 / IGH@ protein / IGL@ protein
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.747 Å
AuthorsJude, K.M. / Garcia, K.C.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01 AI51321 United States
CitationJournal: Nat. Med. / Year: 2018
Title: A human anti-IL-2 antibody that potentiates regulatory T cells by a structure-based mechanism.
Authors: Trotta, E. / Bessette, P.H. / Silveria, S.L. / Ely, L.K. / Jude, K.M. / Le, D.T. / Holst, C.R. / Coyle, A. / Potempa, M. / Lanier, L.L. / Garcia, K.C. / Crellin, N.K. / Rondon, I.J. / Bluestone, J.A.
History
DepositionFeb 15, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 11, 2018Provider: repository / Type: Initial release
Revision 1.1Jul 25, 2018Group: Data collection / Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 2.0Dec 11, 2019Group: Author supporting evidence / Polymer sequence / Category: entity_poly / pdbx_audit_support
Item: _entity_poly.pdbx_seq_one_letter_code_can / _pdbx_audit_support.funding_organization
Revision 2.1Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 2.2Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
H: Fab 5111 heavy chain
L: Fab 5111 light chain
D: Interleukin-2
B: Fab 5111 heavy chain
C: Fab 5111 light chain
A: Interleukin-2
F: Fab 5111 heavy chain
G: Fab 5111 light chain
E: Interleukin-2
J: Fab 5111 heavy chain
K: Fab 5111 light chain
I: Interleukin-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)249,70417
Polymers249,24412
Non-polymers4605
Water5,567309
1
H: Fab 5111 heavy chain
L: Fab 5111 light chain
D: Interleukin-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)62,4034
Polymers62,3113
Non-polymers921
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Fab 5111 heavy chain
C: Fab 5111 light chain
A: Interleukin-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)62,4955
Polymers62,3113
Non-polymers1842
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
F: Fab 5111 heavy chain
G: Fab 5111 light chain
E: Interleukin-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)62,4034
Polymers62,3113
Non-polymers921
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
J: Fab 5111 heavy chain
K: Fab 5111 light chain
I: Interleukin-2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)62,4034
Polymers62,3113
Non-polymers921
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)86.010, 145.730, 107.320
Angle α, β, γ (deg.)90.00, 95.38, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Antibody
Fab 5111 heavy chain


Mass: 23764.600 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q6GMX6
#2: Antibody
Fab 5111 light chain


Mass: 23110.301 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q6IPQ0
#3: Protein
Interleukin-2 / IL-2 / T-cell growth factor / TCGF


Mass: 15435.979 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: IL2 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P60568
#4: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C3H8O3
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 309 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.69 Å3/Da / Density % sol: 54.22 %
Crystal growTemperature: 295 K / Method: vapor diffusion, sitting drop / pH: 5 / Details: 100 mM sodium citrate, pH 5.0, 20% PEG6000

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 0.99987 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: May 11, 2015
RadiationMonochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.99987 Å / Relative weight: 1
ReflectionResolution: 2.747→90 Å / Num. obs: 67865 / % possible obs: 98.9 % / Redundancy: 3.3 % / CC1/2: 0.987 / Rsym value: 0.147 / Net I/σ(I): 8.44
Reflection shellResolution: 2.747→2.91 Å / Redundancy: 3.3 % / Mean I/σ(I) obs: 1 / Num. unique obs: 10720 / CC1/2: 0.396 / Rsym value: 1.163 / % possible all: 96.9

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Processing

Software
NameVersionClassification
PHENIX1.9_1692refinement
XDSMarch 1, 2015data reduction
XDSMarch 1, 2015data scaling
PHASER2.5.6phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entries 3U1S, 4NPY, 3N9G, 4HP0, 2B5I

3u1s

4npy

3n9g

4hp0

2b5i


Resolution: 2.747→86.168 Å / SU ML: 0.43 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.38
RfactorNum. reflection% reflectionSelection details
Rfree0.2475 1973 2.91 %random
Rwork0.1925 ---
obs0.1941 67852 98.85 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.747→86.168 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17235 0 30 309 17574
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00217762
X-RAY DIFFRACTIONf_angle_d0.67924171
X-RAY DIFFRACTIONf_dihedral_angle_d11.1126408
X-RAY DIFFRACTIONf_chiral_restr0.0262790
X-RAY DIFFRACTIONf_plane_restr0.0043048
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.7467-2.81540.40281320.36174417X-RAY DIFFRACTION93
2.8154-2.89160.34631410.29264728X-RAY DIFFRACTION100
2.8916-2.97660.34811430.26834756X-RAY DIFFRACTION100
2.9766-3.07270.2741410.24544754X-RAY DIFFRACTION100
3.0727-3.18260.29531420.23384708X-RAY DIFFRACTION100
3.1826-3.310.31311420.23744722X-RAY DIFFRACTION100
3.31-3.46060.24631410.2114761X-RAY DIFFRACTION100
3.4606-3.64310.27421420.1974680X-RAY DIFFRACTION100
3.6431-3.87130.25961430.18544725X-RAY DIFFRACTION99
3.8713-4.17020.2431400.17324728X-RAY DIFFRACTION99
4.1702-4.58990.18431400.14644705X-RAY DIFFRACTION99
4.5899-5.2540.17461420.14284726X-RAY DIFFRACTION99
5.254-6.61910.21771410.17114741X-RAY DIFFRACTION99
6.6191-86.21060.23741430.17264728X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.36190.2454-0.28071.785-0.02251.3166-0.0567-0.0489-0.07340.1891-0.0472-0.1661-0.10470.12820.07130.2836-0.0212-0.04930.21510.05640.2971-32.0551-3.3733-47.1698
28.35-0.55962.83443.1677-0.12868.52540.0731-0.15930.0501-0.08510.05170.0791-0.1943-0.4749-0.09410.2533-0.0110.0260.2249-0.01320.3137-71.6376-2.8332-57.0523
32.22020.89871.6913.07781.03433.75220.02350.23260.2639-0.0593-0.105-0.2107-0.0486-0.10760.10480.2714-0.01640.03050.43950.10180.3874-31.81814.033-68.0045
43.5132-0.2375-0.77821.05890.69480.5096-0.03510.140.35160.0437-0.0204-0.0502-0.1461-0.02460.0520.38620.01-0.06440.31380.08120.4252-44.14025.3185-65.6232
53.486-0.7898-3.11055.50192.52178.08070.11250.1030.59950.029-0.0009-0.1794-0.52140.083-0.14450.2340.0062-0.07060.34530.08160.5372-60.868510.122-60.7959
63.60990.1836-0.645.3606-1.7071.87430.1515-0.4852-0.12440.2867-0.3371-0.625-0.16550.13630.09910.3513-0.0397-0.12210.44250.03830.5953-7.063910.2134-42.0824
73.93760.7461-0.90533.55040.32165.618-0.0128-0.30140.00350.4318-0.1571-0.58370.11750.49290.09290.3983-0.0422-0.17450.44790.10050.6305-7.47499.9139-39.5401
84.00831.44240.37674.20321.59793.1521-0.1325-0.41580.127-0.29890.0919-0.28430.14230.0691-0.04140.37610.0892-0.0350.3250.0250.2881-33.299154.0218-60.8488
96.0923-0.36480.05071.90211.01332.5362-0.3938-0.3015-1.17260.04950.0391-0.14610.1219-0.07750.32950.3249-0.01330.01350.26850.02950.393-33.204945.4862-53.5845
101.4594-1.93111.5033.31570.15857.38950.22730.3586-0.1193-0.07120.0906-0.390.3809-0.1007-0.21850.29370.0173-0.03110.2194-0.04120.4457-25.861740.4945-60.1374
115.9442-0.3475-0.84971.70330.10411.0617-0.10350.0492-0.64710.00240.016-0.17880.0808-0.02150.04810.3120.0322-0.01910.24860.01190.3192-35.937848.4851-58.2111
125.9819-1.6366-1.86993.551-0.80747.7373-0.14140.0311-0.0761-0.02550.27910.10940.6394-0.1648-0.15910.3520.0214-0.0550.2599-0.0660.4113-68.749550.3181-46.2881
138.32670.0853-3.09974.7696-0.35252.1523-0.2332-0.2715-0.03660.8040.45430.9031-0.0099-0.2724-0.3680.46160.11570.07880.37290.04370.4478-77.181752.4112-44.669
144.2876-1.0613-1.20612.0691-0.12963.7826-0.4797-0.8519-0.16920.41980.2426-0.07970.33960.22070.23840.51660.2318-0.04630.55520.05660.3451-31.907345.2149-36.468
153.16610.0947-0.22271.74320.10080.4395-0.296-0.4374-0.30830.2520.14550.00290.35580.16390.12160.55990.16660.06650.44340.12260.254-46.891743.8761-37.6486
165.03033.59212.88926.86243.22393.5557-0.163-0.2179-0.6380.29230.268-0.18890.25490.1965-0.12280.53160.15220.05680.41130.03390.3939-61.228637.9142-39.2228
173.0628-0.0866-0.32855.8995-2.37665.6005-0.0582-0.18460.02270.15280.0811-0.261-0.03880.47420.03090.2032-0.0261-0.03780.3862-0.02890.5433-9.437134.2678-63.4187
186.34021.089-2.26641.3573-0.28190.8213-1.2807-1.7923-0.83470.50930.52710.9545-0.00930.17890.87860.65710.21420.020.5297-0.00330.7222-16.995836.0499-48.0785
196.2455-1.11991.07533.7734-0.4888.44420.15570.70180.2997-0.25180.0701-0.34160.01030.7732-0.22220.3153-0.019-0.00840.3424-0.03050.4894-8.563332.9789-68.4419
203.50390.0088-0.92792.46990.23092.0663-0.01380.15340.103-0.16480.0041-0.26790.02120.1032-0.06270.40950.0650.06750.29850.02490.3287.53970.0566-5.9074
212.68760.4031-0.09820.98820.05441.12090.07070.3151-0.2829-0.05250.0868-0.20410.0955-0.039-0.13430.44070.07050.03680.3601-0.02340.28073.45850.2871-5.9544
228.26010.8033-4.13242.5168-0.45027.3079-0.2317-0.09030.1602-0.11540.21460.27720.2012-0.0033-0.00090.34530.0817-0.0440.26330.01630.26-32.7326-0.53967.1675
233.55320.1579-2.3363.72210.1494.4626-0.0135-0.3823-0.0342-0.0181-0.0358-0.25270.0410.18120.0830.31960.1056-0.06490.42540.0140.27367.351-7.827814.5761
242.97920.3443-1.09232.63-0.67613.26090.0986-0.3606-0.11710.0691-0.0459-0.34150.0840.1434-0.06370.32730.0319-0.07510.37920.02210.31683.3285-8.198416.3534
256.25510.04593.04243.75290.56956.2497-0.2036-0.1476-0.63670.25090.1751-0.00950.54340.1679-0.04380.32250.07530.0710.29470.07440.4204-23.606-13.2998.655
263.6516-1.43450.99084.9979-1.11252.94110.26680.19720.0584-0.2818-0.0996-0.57070.1236-0.1755-0.08540.4607-0.00080.14150.3858-0.07770.559730.1382-13.2677-13.4629
275.944-0.8930.74015.2491-0.5776.27790.39710.68260.0756-0.1804-0.16-0.2846-0.40690.0887-0.13290.44680.04940.11910.3161-0.06370.408829.2919-12.7108-16.2986
283.9254-0.8321.13772.74260.33384.27090.1280.26960.4482-0.36890.01580.0322-0.3613-0.1121-0.19360.42670.11380.08280.36990.07970.3947-6.351821.9856-3.6835
294.4438-0.63840.7090.99720.49520.6554-0.05450.50040.7654-0.1563-0.0667-0.05460.0371-0.01260.03630.46940.060.11480.31350.11290.4132-1.311520.1177-2.3414
306.7493-0.64381.98034.0471-0.4498.7824-0.367-0.05370.16620.17080.2542-0.7721-0.18460.54620.17840.33740.0740.07170.32090.04270.631734.968618.66495.9177
313.584-0.16090.383.39860.4173.6527-0.3285-0.3259-0.28350.33730.162-0.10360.0689-0.06190.11430.38950.09060.13850.33830.08420.416-4.229424.462218.7467
325.0686-0.17520.372.735-0.07832.3459-0.1272-0.3505-0.10770.5673-0.06380.41620.46410.26010.12680.52780.11740.10530.4040.06160.3273-0.107124.438220.1958
336.43640.7526-3.46985.2235-3.63687.2357-0.4591-0.48090.20080.14160.2223-0.3238-0.19170.12890.25730.43960.1118-0.11170.3498-0.06510.55726.658231.283211.7563
344.24733.2941-0.31044.4072-0.08461.57850.72460.7842-0.6717-0.7275-0.65050.29040.50050.24570.14350.78590.3995-0.17530.7537-0.0830.4916-26.451826.7071-10.1124
353.2733-0.4212-2.10318.61385.25835.56480.1818-0.8207-0.78212.3319-0.41020.54271.37720.30040.08140.932-0.08140.15710.72060.25690.7864-31.053438.77159.6445
363.642-1.95111.66982.8176-2.01382.71781.09381.0160.308-1.6256-0.8817-0.9732-0.2613-0.04940.26611.01570.64970.08460.93950.24310.8082-30.291641.2138-9.0025
378.42563.29563.78295.35375.4215.54050.3285-0.23040.70340.0923-1.3952-0.57750.2767-0.84291.34960.55260.0873-0.0360.4876-0.00710.6751-20.040133.58358.4958
386.4214-1.83880.55551.859-1.39063.99980.94981.4048-0.0963-0.8181-0.90730.088-0.342-0.6414-0.05850.90730.4041-0.01380.93040.0590.5971-30.586735.6063-10.4104
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'H' and (resid 1 through 127 )
2X-RAY DIFFRACTION2chain 'H' and (resid 128 through 224 )
3X-RAY DIFFRACTION3chain 'L' and (resid 1 through 62 )
4X-RAY DIFFRACTION4chain 'L' and (resid 63 through 133 )
5X-RAY DIFFRACTION5chain 'L' and (resid 134 through 215 )
6X-RAY DIFFRACTION6chain 'D' and (resid 5 through 72 )
7X-RAY DIFFRACTION7chain 'D' and (resid 73 through 133 )
8X-RAY DIFFRACTION8chain 'B' and (resid 1 through 33 )
9X-RAY DIFFRACTION9chain 'B' and (resid 34 through 53 )
10X-RAY DIFFRACTION10chain 'B' and (resid 54 through 65 )
11X-RAY DIFFRACTION11chain 'B' and (resid 66 through 127 )
12X-RAY DIFFRACTION12chain 'B' and (resid 128 through 185 )
13X-RAY DIFFRACTION13chain 'B' and (resid 186 through 224 )
14X-RAY DIFFRACTION14chain 'C' and (resid 1 through 62 )
15X-RAY DIFFRACTION15chain 'C' and (resid 63 through 143 )
16X-RAY DIFFRACTION16chain 'C' and (resid 144 through 213 )
17X-RAY DIFFRACTION17chain 'A' and (resid 1 through 72 )
18X-RAY DIFFRACTION18chain 'A' and (resid 73 through 81 )
19X-RAY DIFFRACTION19chain 'A' and (resid 82 through 133 )
20X-RAY DIFFRACTION20chain 'F' and (resid 1 through 65 )
21X-RAY DIFFRACTION21chain 'F' and (resid 66 through 127 )
22X-RAY DIFFRACTION22chain 'F' and (resid 128 through 224 )
23X-RAY DIFFRACTION23chain 'G' and (resid 1 through 62 )
24X-RAY DIFFRACTION24chain 'G' and (resid 63 through 117 )
25X-RAY DIFFRACTION25chain 'G' and (resid 118 through 213 )
26X-RAY DIFFRACTION26chain 'E' and (resid 6 through 72 )
27X-RAY DIFFRACTION27chain 'E' and (resid 73 through 133 )
28X-RAY DIFFRACTION28chain 'J' and (resid 1 through 74 )
29X-RAY DIFFRACTION29chain 'J' and (resid 75 through 127 )
30X-RAY DIFFRACTION30chain 'J' and (resid 128 through 224 )
31X-RAY DIFFRACTION31chain 'K' and (resid 1 through 62 )
32X-RAY DIFFRACTION32chain 'K' and (resid 63 through 117 )
33X-RAY DIFFRACTION33chain 'K' and (resid 118 through 215 )
34X-RAY DIFFRACTION34chain 'I' and (resid 5 through 29 )
35X-RAY DIFFRACTION35chain 'I' and (resid 30 through 44 )
36X-RAY DIFFRACTION36chain 'I' and (resid 45 through 72 )
37X-RAY DIFFRACTION37chain 'I' and (resid 73 through 81 )
38X-RAY DIFFRACTION38chain 'I' and (resid 82 through 133 )

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