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- PDB-5u8q: Structure of the ectodomain of the human Type 1 insulin-like grow... -

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Basic information

Entry
Database: PDB / ID: 5u8q
TitleStructure of the ectodomain of the human Type 1 insulin-like growth factor receptor in complex with IGF-I
Components
  • (Insulin-like growth factor ...) x 2
  • Fv 24-60 heavy chain
  • Fv 24-60 light chain
KeywordsTRANSFERASE / Receptor tyrosine kinase / Type 1 insulin-like growth factor receptor / insulin-like growth-factor I
Function / homology
Function and homology information


glycolate metabolic process / muscle hypertrophy / negative regulation of oocyte development / positive regulation of trophectodermal cell proliferation / insulin-like growth factor binding protein complex / insulin-like growth factor ternary complex / positive regulation of glycoprotein biosynthetic process / cardiac atrium development / negative regulation of cholangiocyte apoptotic process / proteoglycan biosynthetic process ...glycolate metabolic process / muscle hypertrophy / negative regulation of oocyte development / positive regulation of trophectodermal cell proliferation / insulin-like growth factor binding protein complex / insulin-like growth factor ternary complex / positive regulation of glycoprotein biosynthetic process / cardiac atrium development / negative regulation of cholangiocyte apoptotic process / proteoglycan biosynthetic process / positive regulation of steroid hormone biosynthetic process / protein kinase complex / myotube cell development / negative regulation of neuroinflammatory response / positive regulation of transcription regulatory region DNA binding / insulin-like growth factor binding / Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) / skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration / protein transporter activity / bone mineralization involved in bone maturation / positive regulation of cell growth involved in cardiac muscle cell development / IRS-related events triggered by IGF1R / negative regulation of vascular associated smooth muscle cell apoptotic process / negative regulation of muscle cell apoptotic process / exocytic vesicle / cellular response to progesterone stimulus / positive regulation of DNA metabolic process / cellular response to zinc ion starvation / cell activation / cellular response to aldosterone / positive regulation of calcineurin-NFAT signaling cascade / cellular response to testosterone stimulus / insulin receptor complex / transmembrane receptor protein tyrosine kinase activator activity / transcytosis / insulin-like growth factor I binding / negative regulation of hepatocyte apoptotic process / positive regulation of protein-containing complex disassembly / alphav-beta3 integrin-IGF-1-IGF1R complex / response to alkaloid / cell surface receptor signaling pathway via STAT / myoblast differentiation / positive regulation of Ras protein signal transduction / regulation of JNK cascade / positive regulation of insulin-like growth factor receptor signaling pathway / dendritic spine maintenance / myoblast proliferation / insulin binding / growth hormone receptor signaling pathway / negative regulation of interleukin-1 beta production / positive regulation of DNA binding / cellular response to insulin-like growth factor stimulus / muscle organ development / response to L-glutamate / positive regulation of smooth muscle cell migration / establishment of cell polarity / positive regulation of axon regeneration / positive regulation of cardiac muscle hypertrophy / negative regulation of release of cytochrome c from mitochondria / positive regulation of osteoblast proliferation / negative regulation of amyloid-beta formation / positive regulation of cytokinesis / negative regulation of smooth muscle cell apoptotic process / positive regulation of activated T cell proliferation / amyloid-beta clearance / Respiratory syncytial virus (RSV) attachment and entry / insulin receptor substrate binding / negative regulation of tumor necrosis factor production / cellular response to angiotensin / response to vitamin E / Synthesis, secretion, and deacylation of Ghrelin / G-protein alpha-subunit binding / epithelial to mesenchymal transition / negative regulation of MAPK cascade / positive regulation of glycogen biosynthetic process / SHC-related events triggered by IGF1R / phosphatidylinositol 3-kinase binding / positive regulation of osteoblast differentiation / peptidyl-tyrosine autophosphorylation / activation of protein kinase B activity / estrous cycle / cellular response to transforming growth factor beta stimulus / positive regulation of vascular associated smooth muscle cell proliferation / insulin-like growth factor receptor binding / T-tubule / positive regulation of mitotic nuclear division / cerebellum development / cellular response to dexamethasone stimulus / axonogenesis / insulin-like growth factor receptor signaling pathway / platelet alpha granule lumen / hippocampus development / positive regulation of glycolytic process / positive regulation of epithelial cell proliferation / skeletal system development / negative regulation of extrinsic apoptotic signaling pathway / positive regulation of D-glucose import / positive regulation of protein secretion / phosphatidylinositol 3-kinase/protein kinase B signal transduction / response to nicotine
Similarity search - Function
Insulin-like, subunit E / Insulin-like / Insulin-like growth factor I / Insulin-like growth factor / 24 nucleotide stem-loop, u2 snrnp hairpin iv. U2 a'; Chain A / Receptor L-domain / Tyrosine-protein kinase, insulin-like receptor / Tyrosine-protein kinase, receptor class II, conserved site / Receptor tyrosine kinase class II signature. / Alpha-Beta Horseshoe ...Insulin-like, subunit E / Insulin-like / Insulin-like growth factor I / Insulin-like growth factor / 24 nucleotide stem-loop, u2 snrnp hairpin iv. U2 a'; Chain A / Receptor L-domain / Tyrosine-protein kinase, insulin-like receptor / Tyrosine-protein kinase, receptor class II, conserved site / Receptor tyrosine kinase class II signature. / Alpha-Beta Horseshoe / Insulin family / Insulin-like / Insulin/IGF/Relaxin family / Insulin / insulin-like growth factor / relaxin family. / Insulin, conserved site / Insulin family signature. / Insulin-like superfamily / Receptor L-domain / Furin-like cysteine-rich domain / Receptor L-domain superfamily / Furin-like cysteine rich region / Receptor L domain / Furin-like repeat / Furin-like repeats / Growth factor receptor cysteine-rich domain superfamily / : / Fibronectin type 3 domain / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Immunoglobulin-like fold / Protein kinase domain profile. / Protein kinase domain / Immunoglobulins / Protein kinase-like domain superfamily / Immunoglobulin-like / Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
D-MALATE / Insulin-like growth factor 1 / Insulin-like growth factor 1 receptor
Similarity search - Component
Biological speciesHomo sapiens (human)
Mus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.27104018461 Å
AuthorsLawrence, M. / Xu, Y.
CitationJournal: Nat Commun / Year: 2018
Title: How ligand binds to the type 1 insulin-like growth factor receptor.
Authors: Xu, Y. / Kong, G.K. / Menting, J.G. / Margetts, M.B. / Delaine, C.A. / Jenkin, L.M. / Kiselyov, V.V. / De Meyts, P. / Forbes, B.E. / Lawrence, M.C.
History
DepositionDec 14, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 28, 2018Provider: repository / Type: Initial release
Revision 1.1Mar 14, 2018Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title
Revision 2.0Jul 29, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _chem_comp.name / _chem_comp.type / _pdbx_struct_assembly_gen.asym_id_list / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_seq_id / _struct_conn.ptnr2_symmetry
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Oct 4, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag
Revision 2.2Oct 23, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Insulin-like growth factor 1 receptor
B: Insulin-like growth factor I
H: Fv 24-60 heavy chain
L: Fv 24-60 light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)137,01913
Polymers134,4324
Non-polymers2,5879
Water00
1
A: Insulin-like growth factor 1 receptor
B: Insulin-like growth factor I
H: Fv 24-60 heavy chain
L: Fv 24-60 light chain
hetero molecules

A: Insulin-like growth factor 1 receptor
B: Insulin-like growth factor I
H: Fv 24-60 heavy chain
L: Fv 24-60 light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)274,03826
Polymers268,8638
Non-polymers5,17518
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_555-x,-y,z1
Buried area21280 Å2
ΔGint-34 kcal/mol
Surface area107620 Å2
MethodPISA
Unit cell
Length a, b, c (Å)88.690, 197.660, 117.650
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212
Space group name HallP22ab

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Components

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Insulin-like growth factor ... , 2 types, 2 molecules AB

#1: Protein Insulin-like growth factor 1 receptor / Insulin-like growth factor I receptor / IGF-I receptor


Mass: 101085.461 Da / Num. of mol.: 1
Mutation: residues 718-741 are replaced with the sequence AGNN
Source method: isolated from a genetically manipulated source
Details: A17delta-beta construct, see Whitten et al., J. Mol. Biol., v394, pp878-92 (2009).
Source: (gene. exp.) Homo sapiens (human) / Gene: IGF1R / Plasmid: FIII-IGFR.ECD
Details (production host): see Whitten et al., J. Mol. Biol., v394, pp878-92 (2009).
Cell line (production host): Lec8 / Production host: Cricetulus griseus (Chinese hamster)
References: UniProt: P08069, receptor protein-tyrosine kinase
#2: Protein Insulin-like growth factor I / IGF-I / Mechano growth factor / MGF / Somatomedin-C


Mass: 7663.752 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Mature IGF-1 / Source: (gene. exp.) Homo sapiens (human) / Gene: IGF1, IBP1 / Production host: Escherichia coli (E. coli) / References: UniProt: P05019

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Antibody , 2 types, 2 molecules HL

#3: Antibody Fv 24-60 heavy chain


Mass: 13948.477 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: hybridoma / Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: pgpHFT-SUMO-scFV / Production host: Spodoptera frugiperda (fall armyworm)
#4: Antibody Fv 24-60 light chain


Mass: 11733.944 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: hybridoma / Source: (gene. exp.) Mus musculus (house mouse) / Plasmid: pgpHFT-SUMO-scFV / Production host: Spodoptera frugiperda (fall armyworm)

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Sugars , 3 types, 6 molecules

#5: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#6: Polysaccharide alpha-D-mannopyranose-(1-4)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-4)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 748.682 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-4DManpa1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1a_1-5]/1-1-2-2/a4-b1_b4-c1_c4-d1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][a-D-Manp]{[(4+1)][a-D-Manp]{}}}}}LINUCSPDB-CARE
#9: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 3 molecules

#7: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#8: Chemical ChemComp-MLT / D-MALATE / (2R)-2-HYDROXYBUTANEDIOIC ACID / 2-HYDROXY-SUCCINIC ACID


Mass: 134.087 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H6O5

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.84 Å3/Da / Density % sol: 67.94 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 1.2 M ammonium sulfate, 0.1 M imidazole-malate pH 7.0. IGF-1 was included by soaking directly into the mother crystal. For full details see the primary citation.

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.9537 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Aug 20, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9537 Å / Relative weight: 1
ReflectionResolution: 3.27→50 Å / Num. obs: 32228 / % possible obs: 98.6 % / Redundancy: 7.2 % / Biso Wilson estimate: 110.958573147 Å2 / CC1/2: 0.775 / Rmerge(I) obs: 0.19 / Net I/σ(I): 9.1
Reflection shellResolution: 3.27→3.46 Å / Redundancy: 6.7 % / Rmerge(I) obs: 2.07 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 4896 / CC1/2: 0.775 / % possible all: 92.5

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Processing

Software
NameVersionClassification
phenix.refine1.11.1-2575_1692refinement
PHENIX1.11.1-2575_1692refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5U8R

5u8r


Resolution: 3.27104018461→22.1725 Å / SU ML: 0.543083052018 / Cross valid method: FREE R-VALUE / σ(F): 1.32594454353 / Phase error: 38.9050389519
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.303184371053 1524 4.7461849891 %
Rwork0.259826709676 30586 -
obs0.261887829198 32110 98.5725249424 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 177.857907853 Å2
Refinement stepCycle: LAST / Resolution: 3.27104018461→22.1725 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8537 0 167 0 8704
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.001888328003078934
X-RAY DIFFRACTIONf_angle_d0.51966972220212148
X-RAY DIFFRACTIONf_chiral_restr0.04419986906341337
X-RAY DIFFRACTIONf_plane_restr0.003002686560651549
X-RAY DIFFRACTIONf_dihedral_angle_d11.59457349395364
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.271-3.37630.4306593320951310.4146208473552493X-RAY DIFFRACTION89.8322492297
3.3763-3.49650.4153946439581260.354111906282736X-RAY DIFFRACTION98.9968868903
3.4965-3.63590.395193229591490.3347725295162731X-RAY DIFFRACTION99.0712074303
3.6359-3.80060.3342212373461180.3273953984832799X-RAY DIFFRACTION98.9484396201
3.8006-3.99990.340131322961370.2993325354792751X-RAY DIFFRACTION99.1077556623
3.9999-4.24880.3141207008621410.252682472972785X-RAY DIFFRACTION99.3885869565
4.2488-4.57420.2589289923141460.2223510546842786X-RAY DIFFRACTION99.6262317363
4.5742-5.02970.2357703822271550.2023083509792804X-RAY DIFFRACTION99.8313090418
5.0297-5.74640.2546930106031410.2156402634972827X-RAY DIFFRACTION99.7647058824
5.7464-7.19850.3350429455031360.2706702584562879X-RAY DIFFRACTION99.8675057966
7.1985-22.1730.2993476661281440.2518898262882995X-RAY DIFFRACTION99.6824388695
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.260622382791.211649196490.5625063734531.127751345210.9176177161541.821907716020.04448273048522.78641590942-0.759989793321-0.409759345860.453253084058-0.3602628309490.3377980949150.646343069291-0.4663037076342.52285965106-0.03276974008570.207416733571.9333040848-0.3993916783551.2098348284526.242238147429.7959641751-49.9070965647
22.416224651011.28209347549-1.347760854983.05903772868-1.411746698262.99092303732-0.176918781321-0.382894877979-0.1549643930460.151093803521-0.011778922466-0.971817122121-0.2033484332260.116126425640.1401772907062.51834573730.0858730134607-0.05289049700330.850977729714-0.1358974879981.2505908347718.46897190913.2341461151.40061954234
38.34063969760.142141880748-1.233307291153.66353553053-1.072506174356.00277170237-0.0787508962612-0.3619554559091.02607186779-0.03003012453710.3659480826730.524849611954-0.863254506437-0.15465632657-0.2346440078833.15629989950.310328531202-0.02725318819170.8962340990760.09353215244030.6326946884019.53755747653-17.81746557716.43606342494
40.226106473073-0.363161573844-0.003147495487220.6511517018663.020781096912.260370509250.317221175448-0.0310575761470.147466672174-0.337862762643-0.0372439529166-0.02406905964840.05537508574110.289045329251-0.2994846285852.853773710310.08928394821720.09418889818741.5663410456-0.04498574138790.86865663179618.1668807835-23.3123327905-57.9279677721
55.530678545080.618537635538-1.084543918323.63681088215-1.61641643059.79140260422-0.1542449275492.422595742750.1152578714040.3532672851951.24020793598-0.595791678382-0.0652181824344-0.330228469346-0.7493048850612.37047385071-0.0539696845963-0.0696304941051.98659514759-0.204065290520.599162315325-2.1148671219624.6525890177-44.9978350675
68.063337220670.5279949846291.388026113924.60264369988-2.685668368272.795399024780.7178938814940.914804109386-1.179096971-1.47405852601-0.4348383902710.8086222467050.935952402686-0.437846436207-0.1594980430532.275184474310.4934743201730.2641543026661.28603664427-0.1151304008330.9263982041562.8837980373819.9558071461-35.6240861936
72.551026221350.6720856178621.388754060573.259214939854.01784462395.81783230787-0.203679629262-0.981448391784-0.8863960917741.26934451967-0.1187872303070.2376192552890.22744068290.990424026266-0.07069503524852.997962333230.52098051626-0.2308170585421.56819165015-0.171231027755-0.064749205703-3.7378014011427.0094624701-35.3262356704
83.67168017575-4.13357417997-0.8464567200224.908775934471.555644425063.716303928531.50323103572-0.8673656512390.697497802504-0.926392035296-0.3049894477920.372355091422-0.219723764017-0.325655367218-1.039267340311.91349119899-0.0780923608268-0.1595859038670.8563040424050.126214490960.7914233366414.252382156856.2685786603-17.5537103355
94.307254601373.604442184430.1548576521687.56650754083-1.182100347314.36856479844-1.30664301651-0.18019488615-0.441858395439-2.06114056158-0.6577234962920.3799887326431.07315338931-1.025903125031.622803432932.06498387545-0.1597490401310.03190997738490.96785069744-0.1846205871930.75735392765117.362358204452.8656903792-22.8101957998
104.69937147001-1.443364058271.848600283247.67418529505-2.434233727641.161208061410.814680662679-0.0277196663562-0.363877443601-1.52006676255-0.459219952145-0.917096694655-1.625961574571.29516316409-0.4422902030391.62937998998-0.4680518961930.08217803248050.864735046547-0.01975077838950.51607937947823.12332617746.7007145403-15.2554935925
112.192250874193.265513734521.064612901965.165765174452.743930963524.64326698576-0.2780408606860.115652108633-0.84752992448-0.213627044648-0.5280417150050.3948179759140.321601664124-0.2767782511430.1539512825011.79495825978-0.185406707480.465632249562-0.394382100688-0.5481297247591.2725931375422.861776010837.7849803173-27.5043551741
125.71893682585-5.05446378077-5.416542382864.479336206114.801430804055.156935550491.40689822514-1.18264107943-0.4935559385412.47724063974-1.170542680311.545242862241.06747366778-0.560075645948-0.2916029083131.96640095339-0.3543171549240.2642953980441.07132357043-0.06027117051861.0724662653116.125136164337.6774790677-12.2211524149
134.80133757993-6.49889877904-2.634394776999.535036707593.999855031051.72704771249-2.640832504670.237608061072-0.7783942137040.00330656411352-0.9191909279031.549889790810.189622067411-1.334943808923.445248455661.81354999234-0.5169116349570.02280233188041.07374130566-0.2302920702080.95845954547813.223816295742.6397193958-19.8966732133
147.33550462351-5.7067020739-4.613423350226.363852746874.886161005513.76647324662-0.9919080768542.50266386346-1.39020561064-1.61928083729-0.842407241643-0.4850914105011.76311442882-4.700674790861.567094033782.740765415960.116518508086-0.2438818228961.59014566634-0.2290806402360.77661907863116.133448835448.2377371474-25.2920410027
156.1189364877-4.221312124372.933442368741.994157285652.304558036493.938767657480.809227518464-2.26250773089-0.5481979347981.076375249851.032765057950.550004800814-1.22188533732-0.877695833141-0.978900382372.37325523461-0.1226958046120.3951940793761.638432405130.07890536751860.7499941230576.3357119191348.3619268252-8.02776403046
169.20683424338-0.265239992033-1.154451946598.853407360824.795503464012.713141225510.299237224499-1.590508326211.87082101210.896106881324-0.4202471832110.884596791876-0.958819581468-1.069166224530.351855012972.573428594820.202759152561-0.4087433949940.940768871535-0.06556469521050.61818625464419.382978685152.142970797-12.5903479309
172.75587784231.57617321588-2.018979853681.688956490320.8110054369968.92951369237-0.8787396799331.61523946004-0.211401271475-1.363922618680.735281662162-0.4736664258940.5307127416430.2340571234860.1440329401392.11752749853-0.4747615690140.1116851022620.997996966263-0.1120761687541.0332722732723.548349324950.1993779758-14.6840117964
183.59994447722-0.9141866628080.5218043870454.802107467833.680239955897.375768800420.788984510034-0.8626539691541.535159437742.632130582370.1581913846140.982993866745-0.958734934139-0.245782533787-0.9089669861872.49847772403-0.06916494564840.2332065794781.052526686480.2399555804141.0995352359329.92891545546.84620680964.49885506836
195.07763616335-3.44132087933-1.317027030653.60829938531-1.790063363417.346047505970.0577276711624-0.290543960421-0.5144649378131.949351297-0.6686138840680.3983238460861.452965088160.8662743664320.5489321091162.00178211268-0.115604891062-0.01968509823620.984672154820.1341594632520.88821316791835.774565826245.9405861506-0.66789993115
202.483045341990.2902958351940.5649683370372.508798843453.331169471435.02290042790.332051147674-0.283175567428-0.1620020816360.358700027014-0.210518161678-0.114468224809-2.611269488940.994238287873-0.2537199921721.9144494469-0.365398443984-0.1030209081371.372275547480.09813329518550.93321975142538.071976488351.0897134424-8.26682678688
216.395760072226.61803039594-5.713501005176.99756498005-5.966772510795.1645384316-1.1999303146-1.59640038616-1.632067478743.4884488385-1.08167549756-3.03618227932-0.0217548466642-0.7378341658662.104973457121.889379103060.2571068059160.2551183480550.9210115232070.09919996262731.7115673548639.164298537243.5312595221.33550982575
225.30018125456-2.179627410511.767188827279.346191214654.213395430373.9407802318-0.232049136997-0.318554189094-0.499240559316-0.1759442468990.158077871347-1.12480609285-0.1750068912171.202171176140.1645776516332.13153889605-0.1852501539090.01351501404440.9163627551210.02682944768260.77324609243735.842219986655.0995567319-2.07820363312
236.050841302113.53445886385-1.339716898828.581607026520.2655784771321.53681679791-0.0502098808607-1.01031479967-0.4333253441730.5478987017590.213122363980.387182298980.108074866614-0.0887561672515-0.5123310644462.3581989691-0.475074475022-0.02916983909270.9882567037060.4203554240820.72061915717828.834378198746.7010730024-2.1176483633
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 266 )
2X-RAY DIFFRACTION2chain 'A' and (resid 267 through 456 )
3X-RAY DIFFRACTION3chain 'A' and (resid 457 through 575 )
4X-RAY DIFFRACTION4chain 'A' and (resid 576 through 900 )
5X-RAY DIFFRACTION5chain 'B' and (resid 4 through 21 )
6X-RAY DIFFRACTION6chain 'B' and (resid 22 through 53 )
7X-RAY DIFFRACTION7chain 'B' and (resid 54 through 63 )
8X-RAY DIFFRACTION8chain 'H' and (resid 1 through 17 )
9X-RAY DIFFRACTION9chain 'H' and (resid 18 through 25 )
10X-RAY DIFFRACTION10chain 'H' and (resid 26 through 51 )
11X-RAY DIFFRACTION11chain 'H' and (resid 52 through 57 )
12X-RAY DIFFRACTION12chain 'H' and (resid 58 through 64 )
13X-RAY DIFFRACTION13chain 'H' and (resid 65 through 73 )
14X-RAY DIFFRACTION14chain 'H' and (resid 74 through 83 )
15X-RAY DIFFRACTION15chain 'H' and (resid 84 through 91 )
16X-RAY DIFFRACTION16chain 'H' and (resid 92 through 97 )
17X-RAY DIFFRACTION17chain 'H' and (resid 98 through 118 )
18X-RAY DIFFRACTION18chain 'L' and (resid 1 through 13 )
19X-RAY DIFFRACTION19chain 'L' and (resid 14 through 38 )
20X-RAY DIFFRACTION20chain 'L' and (resid 39 through 67 )
21X-RAY DIFFRACTION21chain 'L' and (resid 68 through 75 )
22X-RAY DIFFRACTION22chain 'L' and (resid 76 through 90 )
23X-RAY DIFFRACTION23chain 'L' and (resid 91 through 107 )

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