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Yorodumi- PDB-5t51: Structure of the MIND Complex Shows a Regulatory Focus of Yeast K... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5t51 | |||||||||
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Title | Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly | |||||||||
Components |
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Keywords | CELL CYCLE / kinetochore / complex / chromosome / segregation / MIND / Mis12 | |||||||||
Function / homology | Function and homology information MIS12/MIND type complex / chromosome segregation / kinetochore / mitotic cell cycle / cell division / nucleus Similarity search - Function | |||||||||
Biological species | Kluyveromyces lactis (yeast) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.2007 Å | |||||||||
Authors | Dimitrova, Y. / Jenni, S. / Valverde, R. / Khin, Y. / Harrison, S.C. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Cell / Year: 2016 Title: Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly. Authors: Dimitrova, Y.N. / Jenni, S. / Valverde, R. / Khin, Y. / Harrison, S.C. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5t51.cif.gz | 86.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5t51.ent.gz | 66.3 KB | Display | PDB format |
PDBx/mmJSON format | 5t51.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t5/5t51 ftp://data.pdbj.org/pub/pdb/validation_reports/t5/5t51 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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Details | Dimer confirmed by gel filtration |
-Components
#1: Protein | Mass: 13816.932 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast) Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 Gene: KLLA0_F02343g / Production host: Escherichia coli (E. coli) / References: UniProt: Q6CLK3 |
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#2: Protein | Mass: 12100.684 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (yeast) Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 Gene: KLLA0_E05809g / Production host: Escherichia coli (E. coli) / References: UniProt: Q6CPD1 |
#3: Chemical | ChemComp-SO4 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.22 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop Details: 0.1 M cacodylate, pH 6.5, 2.0 M ammonium sulfate, 0.2 M sodium chloride |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Dec 12, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→35.78 Å / Num. obs: 12402 / % possible obs: 97 % / Redundancy: 6.5 % / CC1/2: 1 / Rmerge(I) obs: 0.119 / Net I/σ(I): 9 |
Reflection shell | Resolution: 2.2007→2.28 Å / Redundancy: 5.5 % / Mean I/σ(I) obs: 1.5 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.2007→35.78 Å / SU ML: 0.24 / Cross valid method: FREE R-VALUE / σ(F): 0.14 / Phase error: 25.59
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Solvent computation | Shrinkage radii: 0.6 Å / VDW probe radii: 0.9 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2007→35.78 Å
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Refine LS restraints |
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LS refinement shell |
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