- PDB-6csv: The structure of the Cep63-Cep152 heterotetrameric complex -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 6csv
Title
The structure of the Cep63-Cep152 heterotetrameric complex
Components
Centrosomal protein of 63 kDa,Centrosomal protein of 152 kDa
Keywords
CELL CYCLE / Centrosome / Complex / Hydrophobic
Function / homology
Function and homology information
negative regulation of protein K63-linked ubiquitination / de novo centriole assembly involved in multi-ciliated epithelial cell differentiation / procentriole / deuterosome / procentriole replication complex / cell projection organization / pericentriolar material / centriole replication / centrosome duplication / centriolar satellite ...negative regulation of protein K63-linked ubiquitination / de novo centriole assembly involved in multi-ciliated epithelial cell differentiation / procentriole / deuterosome / procentriole replication complex / cell projection organization / pericentriolar material / centriole replication / centrosome duplication / centriolar satellite / spindle assembly / signal transduction in response to DNA damage / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / centriole / negative regulation of innate immune response / AURKA Activation by TPX2 / DNA damage checkpoint signaling / spindle pole / Regulation of PLK1 Activity at G2/M Transition / molecular adaptor activity / protein stabilization / cell division / centrosome / protein kinase binding / nucleoplasm / cytosol Similarity search - Function
A: Centrosomal protein of 63 kDa,Centrosomal protein of 152 kDa B: Centrosomal protein of 63 kDa,Centrosomal protein of 152 kDa C: Centrosomal protein of 63 kDa,Centrosomal protein of 152 kDa D: Centrosomal protein of 63 kDa,Centrosomal protein of 152 kDa
Type: DECTRIS PILATUS3 R CdTe 300K-W / Detector: PIXEL / Date: Mar 4, 2016
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.9793 Å / Relative weight: 1
Reflection
Resolution: 2.5→19.93 Å / Num. obs: 19496 / % possible obs: 93.17 % / Redundancy: 4.9 % / Net I/σ(I): 15.82
Reflection shell
Resolution: 2.5→2.54 Å
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0189
refinement
HKL-2000
datacollection
HKL-2000
datareduction
HKL-2000
datascaling
PHENIX
phasing
Coot
modelbuilding
Refinement
Method to determine structure: SAD / Resolution: 2.5→19.926 Å / Cor.coef. Fo:Fc: 0.904 / Cor.coef. Fo:Fc free: 0.867 / SU B: 13.934 / SU ML: 0.3 / Cross valid method: THROUGHOUT / ESU R: 0.798 / ESU R Free: 0.388 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.32079
1555
10 %
RANDOM
Rwork
0.25854
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obs
0.26475
14033
95.71 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å