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- PDB-5t58: Structure of the MIND Complex Shows a Regulatory Focus of Yeast K... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5t58 | ||||||
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Title | Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly | ||||||
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![]() | CELL CYCLE / kinetochore / complex / chromosome / segregation / MIND / Mis12 / Mtw1 complex | ||||||
Function / homology | ![]() MIS12/MIND type complex => GO:0000444 / MIS12/MIND type complex / kinetochore assembly / mitotic sister chromatid segregation / chromosome segregation / kinetochore / spindle pole / cell division / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Dimitrova, Y. / Jenni, S. / Valverde, R. / Khin, Y. / Harrison, S.C. | ||||||
![]() | ![]() Title: Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly. Authors: Dimitrova, Y.N. / Jenni, S. / Valverde, R. / Khin, Y. / Harrison, S.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 319.3 KB | Display | ![]() |
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PDB format | ![]() | 263.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27331.416 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Residue Ala0 is left over from the TEV cleavage site. Source: (gene. exp.) ![]() Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 Gene: KLLA0_F02343g / Production host: ![]() ![]() |
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#2: Protein | Mass: 23916.932 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 Gene: KLLA0_E05809g / Production host: ![]() ![]() |
#3: Protein | Mass: 27843.467 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 Gene: KLLA0_D15741g / Production host: ![]() ![]() |
#4: Protein | Mass: 25183.092 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37 Gene: KLLA0_C15939g / Production host: ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.17 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop Details: 0.1 M Tris-Cl, pH 8.0, 18-22% PEG 550 MME, 0.5-4.5 % poly-gamma-glutamic acid low molecular weight (PGA-LM), 0.2 M potassium thiocyanate, 0.2 M potassium bromide |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Sep 11, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 3.21→86.29 Å / Num. obs: 13484 / % possible obs: 74.4 % / Redundancy: 11.8 % / Rmerge(I) obs: 0.207 / Net I/σ(I): 9.5 |
Reflection shell | Resolution: 3.21→3.33 Å |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.8 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.2131→86.285 Å
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Refine LS restraints |
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LS refinement shell |
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