Entry | Database: PDB / ID: 5t03 |
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Title | Crystal structure of heparan sulfate 6-O-sulfotransferase with bound PAP and glucuronic acid containing hexasaccharide substrate |
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Components | maltose binding protein - heparan sulfate 6-O-sulfotransferase isoform 3 fusion protein |
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Keywords | TRANSFERASE / heparan sulfate / sulfotransferase / complex |
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Function / homology | Function and homology information
heparan sulfate 6-O-sulfotransferase activity / HS-GAG biosynthesis / Transferases; Transferring sulfur-containing groups; Sulfotransferases / heparan sulfate proteoglycan biosynthetic process, enzymatic modification / 3'-phosphoadenosine 5'-phosphosulfate binding / sulfotransferase activity / oligosaccharide binding / detection of maltose stimulus / maltose transport complex / carbohydrate transport ...heparan sulfate 6-O-sulfotransferase activity / HS-GAG biosynthesis / Transferases; Transferring sulfur-containing groups; Sulfotransferases / heparan sulfate proteoglycan biosynthetic process, enzymatic modification / 3'-phosphoadenosine 5'-phosphosulfate binding / sulfotransferase activity / oligosaccharide binding / detection of maltose stimulus / maltose transport complex / carbohydrate transport / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / ATP-binding cassette (ABC) transporter complex / cell chemotaxis / outer membrane-bounded periplasmic space / periplasmic space / DNA damage response / membraneSimilarity search - Function Heparan sulphate 6-sulfotransferase/Protein-tyrosine sulfotransferase / Sulfotransferase / Sulfotransferase family / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / P-loop containing nucleoside triphosphate hydrolaseSimilarity search - Domain/homology alpha-maltotetraose / ADENOSINE-3'-5'-DIPHOSPHATE / P-NITROPHENOL / L(+)-TARTARIC ACID / Heparan-sulfate 6-O-sulfotransferase 3-B / Maltose/maltodextrin-binding periplasmic protein / Maltose/maltodextrin-binding periplasmic proteinSimilarity search - Component |
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Biological species | ![](img/tx_bacteria.gif) Escherichia coli O157:H7 (bacteria)
![](img/tx_fish.gif) Danio rerio (zebrafish) |
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Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å |
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Authors | Pedersen, L.C. / Moon, A.F. / Krahn, J.M. / Liu, J. |
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Funding support | United States, 2items Organization | Grant number | Country |
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National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | GM102137 | United States | National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) | HL094463 | United States |
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Citation | Journal: ACS Chem. Biol. / Year: 2017 Title: Structure Based Substrate Specificity Analysis of Heparan Sulfate 6-O-Sulfotransferases. Authors: Xu, Y. / Moon, A.F. / Xu, S. / Krahn, J.M. / Liu, J. / Pedersen, L.C. |
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History | Deposition | Aug 15, 2016 | Deposition site: RCSB / Processing site: RCSB |
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Revision 1.0 | Feb 1, 2017 | Provider: repository / Type: Initial release |
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Revision 1.1 | Sep 20, 2017 | Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization |
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Revision 1.2 | Dec 4, 2019 | Group: Author supporting evidence / Data collection / Category: chem_comp / pdbx_audit_support Item: _chem_comp.type / _pdbx_audit_support.funding_organization |
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Revision 2.0 | Jul 29, 2020 | Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Structure summary Category: atom_site / chem_comp ...atom_site / chem_comp / entity / entity_name_com / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_molecule_features / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_struct_conn_angle / struct_asym / struct_conn / struct_conn_type / struct_site / struct_site_gen Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_alt_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.occupancy / _atom_site.type_symbol / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_ptnr1_label_alt_id / _struct_conn.pdbx_ptnr2_label_alt_id / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn_type.id Description: Carbohydrate remediation / Provider: repository / Type: Remediation |
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