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- PDB-5o37: Pseudomonas stutzeri PtxB in complex with methylphosphonate (MPn)... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5o37 | ||||||
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Title | Pseudomonas stutzeri PtxB in complex with methylphosphonate (MPn) to 1.37 A resolution | ||||||
![]() | Probable phosphite transport system-binding protein PtxB | ||||||
![]() | TRANSPORT PROTEIN / phosphite / ABC transporters / marine bacteria / PBP / SBP | ||||||
Function / homology | Phosphate/phosphite/phosphonate ABC transporter, periplasmic binding protein / ABC transporter, phosphonate, periplasmic substrate-binding protein / ATP-binding cassette (ABC) transporter complex / transmembrane transport / METHYLPHOSPHONIC ACID ESTER GROUP / Probable phosphite transport system-binding protein PtxB![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bisson, C. / Hitchcock, A. | ||||||
![]() | ![]() Title: The molecular basis of phosphite and hypophosphite recognition by ABC-transporters. Authors: Bisson, C. / Adams, N.B.P. / Stevenson, B. / Brindley, A.A. / Polyviou, D. / Bibby, T.S. / Baker, P.J. / Hunter, C.N. / Hitchcock, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 124.6 KB | Display | ![]() |
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PDB format | ![]() | 96.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.7 KB | Display | ![]() |
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Full document | ![]() | 427 KB | Display | |
Data in XML | ![]() | 13.6 KB | Display | |
Data in CIF | ![]() | 20 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5jvbC ![]() 5lq1C ![]() 5lq5C ![]() 5lq8C ![]() 5lv1C ![]() 5me4C ![]() 5o2jC ![]() 5o2kC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 30173.084 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: C-terminal 6x His-tag / Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-GB / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 46.92 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 4 / Details: 0.1 M MMT buffer pH 4 and 25 % (w/v) PEG 1500 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 5, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92819 Å / Relative weight: 1 |
Reflection | Resolution: 1.37→37.61 Å / Num. obs: 59851 / % possible obs: 99.9 % / Redundancy: 11.2 % / CC1/2: 1 / Rpim(I) all: 0.022 / Net I/σ(I): 16.8 |
Reflection shell | Resolution: 1.37→1.39 Å / Redundancy: 7.4 % / Mean I/σ(I) obs: 1.2 / Num. unique obs: 2940 / CC1/2: 0.485 / Rpim(I) all: 0.615 / % possible all: 98.8 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.115 Å2
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Refinement step | Cycle: 1 / Resolution: 1.37→37.61 Å
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Refine LS restraints |
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