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- PDB-5mmm: Structure of the 70S chloroplast ribosome -

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Basic information

Entry
Database: PDB / ID: 5mmm
TitleStructure of the 70S chloroplast ribosome
DescriptorUncharacterized protein
Ribosome-binding factor PSRP1,chloroplastic/RNA Complex
(50S ribosomal protein ...) x 17
(plastid ribosomal protein ...) x 14
(30S ribosomal protein ...) x 16
KeywordsRIBOSOME / chloroplast / translation / ribosome / cryo-EM
Specimen sourceSpinacia oleracea / plant / spinach / Spinach / ホウレンソウ
MethodElectron microscopy (3.4 Å resolution / Particle / Single particle)
AuthorsBieri, P. / Leibundgut, M. / Saurer, M. / Boehringer, D. / Ban, N.
CitationEMBO J., 2017, 36, 475-486

EMBO J., 2017, 36, 475-486 StrPapers
The complete structure of the chloroplast 70S ribosome in complex with translation factor pY.
Philipp Bieri / Marc Leibundgut / Martin Saurer / Daniel Boehringer / Nenad Ban

Validation Report
SummaryFull reportAbout validation report
DateDeposition: Dec 11, 2016 / Release: Jan 11, 2017
RevisionDateData content typeGroupProviderType
1.0Jan 11, 2017Structure modelrepositoryInitial release
1.1Mar 1, 2017Structure modelDatabase references

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Assembly

Deposited unit
0: 50S ribosomal protein L31
1: 50S ribosomal protein L32, chloroplastic
2: 50S ribosomal protein L33, chloroplastic
3: 50S ribosomal protein L34, chloroplastic
4: 50S ribosomal protein L35, chloroplastic
5: 50S ribosomal protein L36, chloroplastic
6: plastid ribosomal protein cL37, PSRP5
7: 50S ribosomal protein 6, chloroplastic
A: 23S ribosomal RNA
B: 5S ribosomal RNA
C: 50S ribosomal protein L2, chloroplastic
D: plastid ribosomal protein uL3c
E: plastid ribosomal protein uL4c
F: plastid ribosomal protein uL5c
G: plastid ribosomal protein uL6c
H: plastid ribosomal protein bL9c
I: plastid ribosomal protein uL10c
J: 50S ribosomal protein L11, chloroplastic
K: 50S ribosomal protein L13, chloroplastic
L: 50S ribosomal protein L14, chloroplastic
M: plastid ribosomal protein uL15c
N: 50S ribosomal protein L16, chloroplastic
O: plastid ribosomal protein uL14c
P: plastid ribosomal protein uL18c
Q: 50S ribosomal protein L19, chloroplastic
R: 50S ribosomal protein L20, chloroplastic
S: 50S ribosomal protein L21, chloroplastic
T: 50S ribosomal protein L22, chloroplastic
U: 50S ribosomal protein L23, chloroplastic
V: plastid ribosomal protein uL24c
W: 4.5S ribosomal RNA
X: plastid ribosomal protein bL27c
Y: plastid ribosomal protein bL28c
Z: plastid ribosomal protein uL29c
z: tRNA-Phe
8: plastid ribosomal protein bS1c
a: 16S ribosomal RNA
b: 30S ribosomal protein S2, chloroplastic
c: 30S ribosomal protein S3, chloroplastic
d: 30S ribosomal protein S4, chloroplastic
e: 30S ribosomal protein S5, chloroplastic
f: plastid ribosomal protein bS6c
g: 30S ribosomal protein S7, chloroplastic
h: 30S ribosomal protein S8, chloroplastic
i: plastid ribosomal protein uS9c
j: plastid ribosomal protein uS10c
k: 30S ribosomal protein S11, chloroplastic
l: 30S ribosomal protein S12, chloroplastic
m: plastid ribosomal protein uS13c
n: 30S ribosomal protein S14, chloroplastic
o: 30S ribosomal protein S15, chloroplastic
p: 30S ribosomal protein S16, chloroplastic
q: plastid ribosomal protein uS17c
r: 30S ribosomal protein S18, chloroplastic
s: 30S ribosomal protein S19 alpha, chloroplastic
t: plastid ribosomal protein bS20c
u: plastid ribosomal protein bS21c
v: 30S ribosomal protein 2, chloroplastic
w: 30S ribosomal protein 3, chloroplastic
x: 30S ribosomal protein S31, chloroplastic
y: Ribosome-binding factor PSRP1, chloroplastic
hetero molecules


Theoretical massNumber of molelcules
Total (without water)2,618,916842
Polyers2,599,85161
Non-polymers19,064781
Water0
#1


  • idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area (Å2)306490
ΔGint (kcal/M)-2466
Surface area (Å2)837060
MethodPISA

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Components

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50S ribosomal protein ... , 17 types, 17 molecules 0123457CJK...

#1: Polypeptide(L)50S ribosomal protein L31 / plastid ribosomal protein bL31c


Mass: 14748.201 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9R0R6

Cellular component

Molecular function

Biological process

#2: Polypeptide(L)50S ribosomal protein L32, chloroplastic / plastid ribosomal protein bL32c


Mass: 6650.969 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P28804

Cellular component

Molecular function

Biological process

#3: Polypeptide(L)50S ribosomal protein L33, chloroplastic / plastid ribosomal protein bL33c


Mass: 7668.121 Da / Num. of mol.: 1 / Details: Zn-binding protein / Source: (natural) Spinacia oleracea / References: UniProt: P28805

Cellular component

Molecular function

Biological process

#4: Polypeptide(L)50S ribosomal protein L34, chloroplastic / plastid ribosomal protein bL34c / CL34


Mass: 16126.633 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P82244

Cellular component

Molecular function

Biological process

#5: Polypeptide(L)50S ribosomal protein L35, chloroplastic / plastid ribosomal protein bL35c / CL35


Mass: 17376.322 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P23326

Cellular component

Molecular function

Biological process

#6: Polypeptide(L)50S ribosomal protein L36, chloroplastic / plastid ribosomal protein bL36c


Mass: 4414.462 Da / Num. of mol.: 1 / Details: Zn-binding protein / Source: (natural) Spinacia oleracea / References: UniProt: P12230

Cellular component

Molecular function

Biological process

#8: Polypeptide(L)50S ribosomal protein 6, chloroplastic / plastid ribosomal protein cL38 / PSRP6 / CL25 / Plastid-specific 50S ribosomal protein 6 / PSRP-6


Mass: 12080.688 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P82411

Cellular component

Molecular function

Biological process

#11: Polypeptide(L)50S ribosomal protein L2, chloroplastic / plastid ribosomal protein uL2c / Ribosomal protein CS-L4


Mass: 29853.623 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P06509

Cellular component

Molecular function

Biological process

#18: Polypeptide(L)50S ribosomal protein L11, chloroplastic / plastid ribosomal protein uL11c / CL11


Mass: 23689.980 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P31164

Cellular component

Molecular function

Biological process

#19: Polypeptide(L)50S ribosomal protein L13, chloroplastic / plastid ribosomal protein uL13c / CL13


Mass: 28211.629 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P12629

Cellular component

Molecular function

Biological process

#20: Polypeptide(L)50S ribosomal protein L14, chloroplastic / Ribosomal protein CS-L29


Mass: 13484.741 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P09596

Cellular component

Molecular function

Biological process

#22: Polypeptide(L)50S ribosomal protein L16, chloroplastic / plastid ribosomal protein uL15c / Ribosomal protein CS-L24


Mass: 15328.068 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P17353

Cellular component

Molecular function

Biological process

#25: Polypeptide(L)50S ribosomal protein L19, chloroplastic / plastid ribosomal protein bL19c / CL19


Mass: 26121.254 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P82413

Cellular component

Molecular function

Biological process

#26: Polypeptide(L)50S ribosomal protein L20, chloroplastic / plastid ribosomal protein bL20c


Mass: 15725.687 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P28803

Cellular component

Molecular function

Biological process

#27: Polypeptide(L)50S ribosomal protein L21, chloroplastic / plastid ribosomal protein bL21c / CL21 / CS-L7


Mass: 28441.730 Da / Num. of mol.: 1
Details: Residues 68-81 were built as poly-alanine and deposited as UNK.
Source: (natural) Spinacia oleracea / References: UniProt: P24613

Cellular component

Molecular function

Biological process

#28: Polypeptide(L)50S ribosomal protein L22, chloroplastic / plastid ribosomal protein uL22c / Ribosomal protein CS-L13


Mass: 23292.676 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P09594

Cellular component

Molecular function

Biological process

#29: Polypeptide(L)50S ribosomal protein L23, chloroplastic / plastid ribosomal protein uL23c / PRPL23


Mass: 21832.164 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: Q9LWB5

Cellular component

Molecular function

Biological process

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Plastid ribosomal protein ... , 22 types, 22 molecules 6DEFGHIMOP...

#7: Polypeptide(L)plastid ribosomal protein cL37, PSRP5


Mass: 15694.817 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9S2M7

Cellular component

Molecular function

#12: Polypeptide(L)plastid ribosomal protein uL3c


Mass: 33034.750 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9QEC7

Cellular component

Molecular function

Biological process

#13: Polypeptide(L)plastid ribosomal protein uL4c


Mass: 32541.645 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9RVQ9

Cellular component

Molecular function

Biological process

#14: Polypeptide(L)plastid ribosomal protein uL5c


Mass: 28182.688 Da / Num. of mol.: 1
Details: Residues 39-52 were built as poly-alanine and deposited as UNK.
Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9RJX4

Cellular component

Molecular function

Biological process

#15: Polypeptide(L)plastid ribosomal protein uL6c


Mass: 24516.748 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9R4N9

Cellular component

Molecular function

Biological process

#16: Polypeptide(L)plastid ribosomal protein bL9c


Mass: 22038.904 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9RQ91

Cellular component

Molecular function

Biological process

#17: Polypeptide(L)plastid ribosomal protein uL10c


Mass: 26128.230 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9R3N5

Cellular component

Molecular function

Biological process

#21: Polypeptide(L)plastid ribosomal protein uL15c


Mass: 29081.514 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9QHT0

Cellular component

Molecular function

Biological process

#23: Polypeptide(L)plastid ribosomal protein uL14c


Mass: 14577.172 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9RLJ4

Cellular component

Molecular function

Biological process

#24: Polypeptide(L)plastid ribosomal protein uL18c


Mass: 18410.205 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9QQ60

Cellular component

Molecular function

Biological process

#30: Polypeptide(L)plastid ribosomal protein uL24c


Mass: 21417.945 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9QFU9

Cellular component

Molecular function

Biological process

#32: Polypeptide(L)plastid ribosomal protein bL27c


Mass: 21366.447 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9R4I2

Cellular component

Molecular function

Biological process

#33: Polypeptide(L)plastid ribosomal protein bL28c


Mass: 16460.488 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9RD02

Cellular component

Molecular function

Biological process

#34: Polypeptide(L)plastid ribosomal protein uL29c


Mass: 19083.385 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9R7W8

Cellular component

Molecular function

Biological process

#36: Polypeptide(L)plastid ribosomal protein bS1c


Mass: 14826.253 Da / Num. of mol.: 1
Details: Residues 1001-1068 (OB-fold) and residues 1101-1143 and 1201-1263 (extensions) were built as poly-alanine and deposited as UNK.
Source: (natural) Spinacia oleracea
#42: Polypeptide(L)plastid ribosomal protein bS6c


Mass: 23510.664 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9R0H2

Cellular component

Molecular function

Biological process

#45: Polypeptide(L)plastid ribosomal protein uS9c


Mass: 22415.057 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9RY17

Cellular component

Molecular function

Biological process

#46: Polypeptide(L)plastid ribosomal protein uS10c


Mass: 21440.539 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9RWE7

Cellular component

Molecular function

Biological process

#49: Polypeptide(L)plastid ribosomal protein uS13c


Mass: 19310.641 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9R024

Cellular component

Molecular function

Biological process

#53: Polypeptide(L)plastid ribosomal protein uS17c


Mass: 18151.936 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9RRR0

Cellular component

Molecular function

Biological process

#56: Polypeptide(L)plastid ribosomal protein bS20c


Mass: 20147.291 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea
#57: Polypeptide(L)plastid ribosomal protein bS21c


Mass: 20616.150 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: A0A0K9RHF9

Cellular component

Molecular function

Biological process

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RNA chain , 5 types, 5 molecules ABWza

#9: RNA chain23S ribosomal RNA / plastid 23S rRNA


Mass: 911344.250 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: GenBank: 7636084
#10: RNA chain5S ribosomal RNA / plastid 5S rRNA


Mass: 39014.184 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea
#31: RNA chain4.5S ribosomal RNA / plastid 4.5S rRNA


Mass: 34334.488 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: GenBank: 12299
#35: RNA chaintRNA-Phe


Mass: 24485.539 Da / Num. of mol.: 1
Details: An idealized full-length E-site E.coli tRNA-Phe (76 residues) from PDB 2J00 was fitted into the density and adjusted prior to minimization.
Source: (natural) Spinacia oleracea
#37: RNA chain16S ribosomal RNA / plastid 16S rRNA


Mass: 483466.500 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: GenBank: 7636084

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30S ribosomal protein ... , 16 types, 16 molecules bcdeghklno...

#38: Polypeptide(L)30S ribosomal protein S2, chloroplastic / plastid ribosomal protein uS2c


Mass: 26736.141 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P08242

Cellular component

Molecular function

Biological process

#39: Polypeptide(L)30S ribosomal protein S3, chloroplastic / plastid ribosomal protein uS3c


Mass: 24965.156 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P09595

Cellular component

Molecular function

Biological process

#40: Polypeptide(L)30S ribosomal protein S4, chloroplastic / plastid ribosomal protein uS4c


Mass: 23454.531 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P13788

Cellular component

Molecular function

Biological process

#41: Polypeptide(L)30S ribosomal protein S5, chloroplastic / plastid ribosomal protein uS5c


Mass: 33626.363 Da / Num. of mol.: 1
Details: Residues 122-150 were built as poly-alanine and deposited as UNK.
Source: (natural) Spinacia oleracea / References: UniProt: Q9ST69

Cellular component

Molecular function

Biological process

#43: Polypeptide(L)30S ribosomal protein S7, chloroplastic / plastid ribosomal protein uS7c


Mass: 17378.309 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P82129

Cellular component

Molecular function

Biological process

#44: Polypeptide(L)30S ribosomal protein S8, chloroplastic / plastid ribosomal protein uS8c


Mass: 15527.256 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P09597

Cellular component

Molecular function

Biological process

#47: Polypeptide(L)30S ribosomal protein S11, chloroplastic / plastid ribosomal protein uS11c


Mass: 14927.551 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P06506

Cellular component

Molecular function

Biological process

#48: Polypeptide(L)30S ribosomal protein S12, chloroplastic / plastid ribosomal protein uS12c


Mass: 13794.261 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P62128

Cellular component

Molecular function

Biological process

#50: Polypeptide(L)30S ribosomal protein S14, chloroplastic / plastid ribosomal protein uS14c


Mass: 11809.832 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P06507

Cellular component

Molecular function

Biological process

#51: Polypeptide(L)30S ribosomal protein S15, chloroplastic / plastid ribosomal protein uS15c


Mass: 10778.763 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: Q9M3I4

Cellular component

Molecular function

Biological process

#52: Polypeptide(L)30S ribosomal protein S16, chloroplastic / plastid ribosomal protein bS16c


Mass: 10454.237 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P28807

Cellular component

Molecular function

Biological process

#54: Polypeptide(L)30S ribosomal protein S18, chloroplastic / plastid ribosomal protein bS18c


Mass: 12079.137 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: Q9M3K7

Cellular component

Molecular function

Biological process

#55: Polypeptide(L)30S ribosomal protein S19 alpha, chloroplastic / plastid ribosomal protein uS19c / CS-S23


Mass: 10632.500 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P06508

Cellular component

Molecular function

Biological process

#58: Polypeptide(L)30S ribosomal protein 2, chloroplastic / plastid ribosomal protein cS22 / Plastid-specific 30S ribosomal protein 2 / PSRP-2


Mass: 28327.863 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P82277

Cellular component

Molecular function

#59: Polypeptide(L)30S ribosomal protein 3, chloroplastic / Pplastid ribosomal protein cS23 / plastid-specific 30S ribosomal protein 3 / PSRP-3


Mass: 20312.750 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P82412

Cellular component

Molecular function

Biological process

#60: Polypeptide(L)30S ribosomal protein S31, chloroplastic / plastid ribosomal protein bTHXc / Plastid-specific 30S ribosomal protein 4 / PSRP-4


Mass: 10602.076 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P47910

Cellular component

Molecular function

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Polypeptide(L) , 1 types, 1 molecules y

#61: Polypeptide(L)Ribosome-binding factor PSRP1, chloroplastic / plastid pY / 30S ribosomal protein 1 / CS-S5 / CS5 / Plastid-specific 30S ribosomal protein 1 / PSrp-1 / Ribosomal protein 1 / S22


Mass: 33799.266 Da / Num. of mol.: 1 / Source: (natural) Spinacia oleracea / References: UniProt: P19954

Cellular component

Molecular function

Biological process

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Non-polymers , 2 types, 781 molecules

#62: ChemicalChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Formula: Zn
#63: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 779 / Formula: Mg

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: SINGLE PARTICLE

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Sample preparation

ComponentName: Chloroplast 70S ribosome / Type: RIBOSOME
Entity ID: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61
Source: NATURAL
Molecular weightValue: 2.4 deg. / Units: MEGADALTONS / Experimental flag: NO
Source (natural)Organelle: chloroplast / Organism: Spinacia oleracea / Tissue: leaf
Buffer solutionpH: 7.6
Buffer component
IDConc.Conc. unitsNameFormulaBuffer ID
125mMTris hydrochlorideTris-HCl1
225mMPotassium chlorideKCl1
325mMMagnesium acetateMg(CH3COO)21
42mMDithiothreitolDTT1
50.05mMSpermine1
62mMSpermidine1
SpecimenConc.: 0.12 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid type: Quantifoil R2/2
VitrificationInstrument: FEI VITROBOT MARK I / Cryogen name: ETHANE-PROPANE / Humidity: 100 % / Chamber temperature: 278 kelvins

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal magnification: 59000 / Nominal defocus max: 3500 nm / Nominal defocus min: 800 nm / Cs: 2.7 mm
Specimen holderSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingAverage exposure time: 0.77 sec. / Electron dose: 20 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON II (4k x 4k) / Number of real images: 2796
Image scansMovie frames/image: 8 / Used frames/image: 2-8

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Processing

EM software
IDNameVersionCategoryDetailsImage processing IDImaging IDFitting ID
1EMAN1.9PARTICLE SELECTIONBatchboxer1
2FEI EPU SoftwareIMAGE ACQUISITION1
4CTFFIND3CTF CORRECTIONCTF estimation1
5RELION1.4CTF CORRECTIONAmplitude correction1
8Chimera1.11.2MODEL FITTING1
10RELION1.4INITIAL EULER ASSIGNMENT1
11RELION1.4FINAL EULER ASSIGNMENT1
12RELION1.4CLASSIFICATION1
13RELION1.4RECONSTRUCTION1
14PHENIX1.11.1MODEL REFINEMENT1
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNumber of particles selected: 326094
SymmetryPoint symmetry: C1
3D reconstructionResolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 140583 / Symmetry type: POINT
Atomic model buildingOverall b value: 90.8 / Ref protocol: OTHER / Ref space: RECIPROCAL

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