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Yorodumi- PDB-5h43: Structural and mechanistical studies of the nuclear import by Imp... -
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-Basic information
Entry | Database: PDB / ID: 5h43 | ||||||
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Title | Structural and mechanistical studies of the nuclear import by Importin-alpha | ||||||
Components |
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Keywords | PROTEIN TRANSPORT/TRANSFERASE / importin alpha / nuclear import / PROTEIN TRANSPORT-TRANSFERASE complex | ||||||
Function / homology | Function and homology information Sensing of DNA Double Strand Breaks / MSL complex / regulation of DNA recombination / histone H4K16 acetyltransferase activity / entry of viral genome into host nucleus through nuclear pore complex via importin / regulation of mRNA processing / positive regulation of viral life cycle / NS1 Mediated Effects on Host Pathways / myeloid cell differentiation / NLS-dependent protein nuclear import complex ...Sensing of DNA Double Strand Breaks / MSL complex / regulation of DNA recombination / histone H4K16 acetyltransferase activity / entry of viral genome into host nucleus through nuclear pore complex via importin / regulation of mRNA processing / positive regulation of viral life cycle / NS1 Mediated Effects on Host Pathways / myeloid cell differentiation / NLS-dependent protein nuclear import complex / host cell / NSL complex / : / nuclear import signal receptor activity / CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde / nuclear localization sequence binding / DNA metabolic process / peptide-lysine-N-acetyltransferase activity / CaMK IV-mediated phosphorylation of CREB / NLS-bearing protein import into nucleus / Formation of WDR5-containing histone-modifying complexes / MLL1 complex / histone acetyltransferase complex / histone acetyltransferase / transcription initiation-coupled chromatin remodeling / neurogenesis / regulation of autophagy / transcription coregulator activity / ISG15 antiviral mechanism / kinetochore / nuclear matrix / protein import into nucleus / histone deacetylase binding / SARS-CoV-1 activates/modulates innate immune responses / HATs acetylate histones / nuclear membrane / Estrogen-dependent gene expression / RNA polymerase II-specific DNA-binding transcription factor binding / transcription coactivator activity / Golgi membrane / negative regulation of DNA-templated transcription / endoplasmic reticulum membrane / SARS-CoV-2 activates/modulates innate and adaptive immune responses / positive regulation of DNA-templated transcription / enzyme binding / positive regulation of transcription by RNA polymerase II / RNA binding / nucleoplasm / membrane / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Wang, R. / Li, Y. | ||||||
Funding support | China, 1items
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Citation | Journal: Traffic / Year: 2018 Title: Structural insights into the nuclear import of the histone acetyltransferase males-absent-on-the-first by importin alpha 1 Authors: Zheng, W. / Wang, R. / Liu, X. / Tian, S. / Yao, B. / Chen, A. / Jin, S. / Li, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5h43.cif.gz | 103.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5h43.ent.gz | 78.5 KB | Display | PDB format |
PDBx/mmJSON format | 5h43.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h4/5h43 ftp://data.pdbj.org/pub/pdb/validation_reports/h4/5h43 | HTTPS FTP |
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-Related structure data
Related structure data | 3kndS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 46359.945 Da / Num. of mol.: 1 / Fragment: UNP residues 70-497 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KPNA2, RCH1, SRP1 / Plasmid: pET24a / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P52292 |
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#2: Protein/peptide | Mass: 1328.500 Da / Num. of mol.: 1 / Fragment: UNP residues 140-149 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KAT8, MOF, MYST1 / Plasmid: pET24a / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9H7Z6, histone acetyltransferase |
#3: Protein/peptide | Mass: 1801.974 Da / Num. of mol.: 1 / Fragment: UNP residues 128-142 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KAT8, MOF, MYST1 / Plasmid: pET24a / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q9H7Z6, histone acetyltransferase |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.38 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop Details: 3%(v/v) Tacsimate pH5.0, 0.1M Sodium citrate tribasic dehydrate pH5.6, 16%(w/v) PEG 3350 PH range: 5.0-5.6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 1.005 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jun 21, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.005 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50 Å / Num. obs: 34657 / % possible obs: 99.9 % / Redundancy: 18.6 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 34 |
Reflection shell | Resolution: 2.31→2.37 Å / Mean I/σ(I) obs: 5.7 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3KND Resolution: 2.3→47.9 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.951 / SU B: 8.164 / SU ML: 0.182 / Cross valid method: THROUGHOUT / ESU R: 0.287 / ESU R Free: 0.215 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 68.447 Å2
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Refinement step | Cycle: 1 / Resolution: 2.3→47.9 Å
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Refine LS restraints |
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