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Open data
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Basic information
Entry | Database: PDB / ID: 6iw8 | ||||||
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Title | Crystal structure of Importin-alpha and wild-type GM130 | ||||||
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![]() | PROTEIN BINDING / Importin alpha / GM130 | ||||||
Function / homology | ![]() meiotic spindle assembly / Golgi cis cisterna / positive regulation of protein glycosylation / Golgi disassembly / asymmetric cell division / Golgi ribbon formation / Sensing of DNA Double Strand Breaks / entry of viral genome into host nucleus through nuclear pore complex via importin / positive regulation of viral life cycle / microtubule nucleation ...meiotic spindle assembly / Golgi cis cisterna / positive regulation of protein glycosylation / Golgi disassembly / asymmetric cell division / Golgi ribbon formation / Sensing of DNA Double Strand Breaks / entry of viral genome into host nucleus through nuclear pore complex via importin / positive regulation of viral life cycle / microtubule nucleation / importin-alpha family protein binding / NLS-dependent protein nuclear import complex / cis-Golgi network / postsynapse to nucleus signaling pathway / Golgi Cisternae Pericentriolar Stack Reorganization / centrosome cycle / COPII-mediated vesicle transport / syntaxin binding / nuclear import signal receptor activity / nuclear localization sequence binding / Golgi cisterna membrane / NLS-bearing protein import into nucleus / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / protein glycosylation / COPII-coated ER to Golgi transport vesicle / Golgi organization / mitotic spindle assembly / spindle assembly / endoplasmic reticulum to Golgi vesicle-mediated transport / COPI-mediated anterograde transport / negative regulation of autophagy / endoplasmic reticulum-Golgi intermediate compartment membrane / negative regulation of protein binding / mitotic spindle / spindle pole / cytoplasmic stress granule / protein import into nucleus / host cell / protein transport / microtubule binding / protein homotetramerization / DNA-binding transcription factor binding / microtubule / postsynaptic density / cadherin binding / Golgi membrane / glutamatergic synapse / protein kinase binding / Golgi apparatus / nucleoplasm / identical protein binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chang, C.-C. / Chen, C.-J. / Pien, Y.-C. / Tsai, S.-Y. / Hsia, K.-C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-alpha. Authors: Chang, C.-C. / Chen, C.-J. / Grauffel, C. / Pien, Y.-C. / Lim, C. / Tsai, S.-Y. / Hsia, K.-C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 187.5 KB | Display | ![]() |
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PDB format | ![]() | 146.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.7 KB | Display | ![]() |
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Full document | ![]() | 468.4 KB | Display | |
Data in XML | ![]() | 20.1 KB | Display | |
Data in CIF | ![]() | 27.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6iwaC ![]() 6k06C ![]() 1iq1S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 5769.612 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein | Mass: 46898.656 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.54 Å3/Da / Density % sol: 51.54 % |
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Crystal grow | Temperature: 293 K / Method: evaporation Details: 0.1M Sodium malonate, 12% w/v Polyethylene glycol 3350 (pH 7.0) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: Feb 24, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→20 Å / Num. obs: 13194 / % possible obs: 98.7 % / Redundancy: 5.5 % / CC1/2: 0.996 / Rsym value: 0.097 / Net I/σ(I): 16.7 |
Reflection shell | Resolution: 2.8→2.9 Å / Num. unique obs: 1355 / CC1/2: 0.909 / Rsym value: 0.546 / % possible all: 91.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1IQ1 Resolution: 2.8→20 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 2.8→20 Å
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LS refinement shell | Highest resolution: 2.8 Å
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Refinement TLS params. | Method: refined / Origin x: -0.6384 Å / Origin y: 11.1066 Å / Origin z: -22.5291 Å
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Refinement TLS group | Selection details: all |