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Open data
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Basic information
Entry | Database: PDB / ID: 5gs1 | |||||||||
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Title | Crystal structure of homo-specific diabody | |||||||||
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![]() | IMMUNE SYSTEM / diabody / antibody fragment | |||||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Kim, J.H. / Song, D.H. / Youn, S.J. / Kim, J.W. / Cho, G. / Lee, H. / Lee, J.O. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface. Authors: Kim, J.H. / Song, D.H. / Youn, S.J. / Kim, J.W. / Cho, G. / Kim, S.C. / Lee, H. / Jin, M.S. / Lee, J.O. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 633.5 KB | Display | ![]() |
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PDB format | ![]() | 519.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 575.5 KB | Display | ![]() |
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Full document | ![]() | 617.5 KB | Display | |
Data in XML | ![]() | 125.3 KB | Display | |
Data in CIF | ![]() | 177.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5gruC ![]() 5grvC ![]() 5grwC ![]() 5grxC ![]() 5gryC ![]() 5grzC ![]() 5gs0C ![]() 5gs2C ![]() 5gs3C ![]() 2v7nS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 11654.840 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Antibody | Mass: 13232.579 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #3: Antibody | Mass: 24869.445 Da / Num. of mol.: 10 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.07 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: 0.1 M Sodium citrate pH 4.6, 0.2 M Ammonium sulfate, 18% PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 20, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 248167 / % possible obs: 96.6 % / Redundancy: 5.1 % / Net I/σ(I): 18.6 |
Reflection shell | Resolution: 2→2.07 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2V7N Resolution: 2→20.046 Å / SU ML: 0.27 / Cross valid method: FREE R-VALUE / σ(F): 1.44 / Phase error: 35.45 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→20.046 Å
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Refine LS restraints |
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LS refinement shell |
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