RNA polymerase subunit, found in RNA polymerase complexes I, II, and III / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerase subunit beta / RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III / Transcription elongation factor SPT4 / RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / General transcription elongation factor TFIIS / RNA polymerase II subunit B32 / Transcription elongation factor SPT5 ...RNA polymerase subunit, found in RNA polymerase complexes I, II, and III / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerase subunit beta / RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III / Transcription elongation factor SPT4 / RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / General transcription elongation factor TFIIS / RNA polymerase II subunit B32 / Transcription elongation factor SPT5 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / RNA polymerase II subunit B12.5 / DNA-directed RNA polymerase subunit / RNA polymerase II third largest subunit B44, part of central core / DNA-directed RNA polymerase subunit Similarity search - Component
Journal: Science / Year: 2017 Title: Structure of the complete elongation complex of RNA polymerase II with basal factors. Authors: Haruhiko Ehara / Takeshi Yokoyama / Hideki Shigematsu / Shigeyuki Yokoyama / Mikako Shirouzu / Shun-Ichi Sekine / Abstract: In the early stage of transcription, eukaryotic RNA polymerase II (Pol II) exchanges initiation factors with elongation factors to form an elongation complex for processive transcription. Here we ...In the early stage of transcription, eukaryotic RNA polymerase II (Pol II) exchanges initiation factors with elongation factors to form an elongation complex for processive transcription. Here we report the structure of the Pol II elongation complex bound with the basal elongation factors Spt4/5, Elf1, and TFIIS. Spt4/5 (the Spt4/Spt5 complex) and Elf1 modify a wide area of the Pol II surface. Elf1 bridges the Pol II central cleft, completing a "DNA entry tunnel" for downstream DNA. Spt4 and the Spt5 NGN and KOW1 domains encircle the upstream DNA, constituting a "DNA exit tunnel." The Spt5 KOW4 and KOW5 domains augment the "RNA exit tunnel," directing the exiting nascent RNA. Thus, the elongation complex establishes a completely different transcription and regulation platform from that of the initiation complexes.
History
Deposition
May 29, 2017
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Header (metadata) release
Aug 16, 2017
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Map release
Aug 16, 2017
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Update
Mar 27, 2024
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Current status
Mar 27, 2024
Processing site: PDBj / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
MFFLKDLSLI LTLHPSYFGP QMNQYLREKL LTDVEGTCTG QFGYIVTVLD GMNIDVGKGR IIPGSGSAEF EVKYRAVVWK PFKGEVVDA IVSNVSPIGF FADVGPLNVF VSTRLIPDNL VYNPSNSPPA YMSNDELITK GSKVRLKVVG TRTDVNEIYA I GSIKEDFL GAI
UniProtKB: DNA-directed RNA polymerase subunit
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Macromolecule #8: RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, ...
Macromolecule
Name: RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Macromolecule #10: RNA polymerase subunit ABC10-beta, common to RNA polymerases I, I...
Macromolecule
Name: RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Macromolecule #12: RNA polymerase subunit, found in RNA polymerase complexes I, II, ...
Macromolecule
Name: RNA polymerase subunit, found in RNA polymerase complexes I, II, and III type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
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